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Gene information
General information
TC011548 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3780847..3784824
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011548
    iBeetle screen iB_06552

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011548 RA
      MNRYITLHQ LGDGTYGSV VLGQRKDTG EKVAIKRMK 
      RKYYSWDEA MNLREVKSL KKLHHSNVV KLKEVIREN 
      DVLYFVFEY MQENLYQLI KDRRVPLPE ATVRNMLYQ 
      ILQGLAFIH RHGFFHRDL KPENILCSG PDLIKIADF 
      GLVREIRSR PPYTDYVST RWYRAPEVL LHSTTYSSP 
      IDLWAVGCI AAEIYTFRP LFPGTTETD QLYKICAIL 
      GTPDRKQWP ECYQLAGAV GFKFPYFTK TPLGDVVPQ 
      ANAAAIDLM DSFLSWNPA HRPTAQSAL KHQYFQVGQ 
      QYNVIHSGV RQNQANQMQ TQPVVIKNE QMFPSKPSA 
      RTTRNQYNQ QMMAPPLQP SVQINIQSL IKPMQEKPQ 
      NPPIVYSFG QSDFANKFF PGNNKANTN NNNKKISWG 
      LDDTIDYKE DDFADILGS KIKTHATDK SKENKINLS 
      QYNLQDILE PLAKASAGT ARSQYLSVS RYVAGQPSG 
      VRRDSNISL MSSFSTSRK DSGLYSNKP SKVELPSIW 
      NDPLTSLTS SKQRPIENV NNIAADWLN K
    • >TC011548 RB
      MNRYITLHQ LGDGTYGSV VLGQRKDTG EKVAIKRMK 
      RKYYSWDEA MNLREVKSL KKLHHSNVV KLKEVIREN 
      DVLYFVFEY MQENLYQLI KDRRVPLPE ATVRNMLYQ 
      ILQGLAFIH RHGFFHRDL KPENILCSG PDLIKIADF 
      GLVREIRSR PPYTDYVST RWYRAPEVL LHSTTYSSP 
      IDLWAVGCI AAEIYTFRP LFPGTTETD QLYKICAIL 
      GTPDRKQWP ECYQLAGAV GFKFPYFTK TPLGDVVPQ 
      ANAAAIDLM DSFLSWNPA HRPTAQSAL KHQYFQVGQ 
      QYNVIHSGV RQNQANQMQ TQPVVIKNE QMFPSKPSA 
      RTTRNQYNQ QMMAPPLQP SVQINIQSL IKPMQEKPQ 
      NPPIVYSFG QSDFANKFF PGNNKANTN NNNKKISWG 
      LDDTIDYKE DDFADILGS KIKTHATDK SKENKINLS 
      QYNLQDILE PLAKASAGT ARSQYLSVS RKDSGLYSN 
      KPSKVELPS IWNDPLTSL TSSKQRPIE NVNNIAADW 
      LNK
    • >TC011548 RC
      MNRYITLHQ LGDGTYGSV VLGQRKDTG EKVAIKRMK 
      RKYYSWDEA MNLREVKSL KKLHHSNVV KLKEVIREN 
      DVLYFVFEY MQENLYQLI KDRRVPLPE ATVRNMLYQ 
      ILQGLAFIH RHGFFHRDL KPENILCSG PDLIKIADF 
      GLVREIRSR PPYTDYVST RWYRAPEVL LHSTTYSSP 
      IDLWAVGCI AAEIYTFRP LFPGTTETD QLYKICAIL 
      GTPDRKQWP ECYQLAGAV GFKFPYFTK TPLGDVVPQ 
      ANAAAIDLM DSFLSWNPA HRPTAQSAL KHQYFQVGQ 
      QYNVIHSGV RQNQANQMQ TQPVVIKNE QMFPSKPSA 
      RTTRNQYNQ QMMAPPLQP SVQINIQSL IKPMQEKPQ 
      NPPIVYSFG QSDFANKFF PGNNKANTN NNNKKISWG 
      LDDTIDYKE DDFADILGK IKTHATDKS KENKINLSQ 
      YNLQDILEP LAKASAGTA RSQYLSVSR YVAGQPSGV 
      RRDSNISLM SSFSTSRKD SGLYSNKPS KVELPSIWN 
      DPLTSLTSS KQRPIENVN NIAADWLNK
    CDS sequence
    • >TC011548 RA
      atgaatcgc tatattact cttcatcag ttaggagat 
      ggcacgtat gggtcggtg gttttggga caaagaaag 
      gatacggga gagaaggtg gcgatcaaa aggatgaaa 
      aggaaatac tactcctgg gatgaggcc atgaattta 
      agagaggtc aagtctcta aaaaaacta caccattcc 
      aatgttgtc aaactcaaa gaagtgata agagaaaat 
      gacgtcttg tactttgtt tttgagtac atgcaagaa 
      aatttgtac cagttgatc aaagatcgc cgagtgcct 
      ttaccagaa gcaacagtg aggaacatg ctttatcaa 
      attctacaa ggcctagcc ttcattcac aggcatggc 
      ttctttcac cgcgactta aaacccgaa aacatcctt 
      tgctcaggc cccgacttg atcaaaatc gccgatttt 
      ggtttagtc cgggaaatc cgctcccgt cctccttac 
      actgactac gtttcaacc cgatggtac cgcgcccct 
      gaagtgctc ctccattcc acgacctac agcagcccc 
      attgacctc tgggccgtc ggatgcata gcagccgaa 
      atatacacc ttccgaccg ttatttccc gggaccaca 
      gaaacagac caattgtac aaaatatgt gccattctt 
      ggaactcct gacaggaaa caatggccg gagtgttac 
      caactagcc ggcgctgtg gggttcaag ttcccgtat 
      ttcaccaaa acaccactt ggggatgtg gtgccccaa 
      gcaaatgcg gcagcgatt gatctaatg gacagcttc 
      ttgagctgg aatcctgcc cacagaccg actgctcaa 
      tccgcgctc aaacaccag tactttcag gtgggccaa 
      cagtacaac gtcatacac tctggagtt cgtcaaaac 
      caagccaat caaatgcaa acgcaaccc gttgtgata 
      aaaaatgaa caaatgttt ccgtccaag ccaagtgca 
      cggactact cgaaaccag tacaatcag caaatgatg 
      gcgcctccg cttcagccg tctgtgcaa atcaacatt 
      caatcgttg atcaaacca atgcaggag aagccgcaa 
      aatccgccc attgtttac agtttcggg cagtctgac 
      tttgcgaat aagtttttt cctgggaat aataaagca 
      aatacgaat aacaataat aagaaaata tcgtggggg 
      ttggatgat acaatagat tacaaagag gacgatttt 
      gcggatatt ttaggaagt aaaattaaa actcacgct 
      acggataaa tcaaaggaa aacaaaatt aacttgagc 
      caatacaat ctccaagat atcctggaa cccctagct 
      aaggcaagc gcagggact gcaaggagt caatatttg 
      agtgtttcg aggtatgta gctggacaa ccctctggt 
      gttcggaga gactcaaat atcagtctt atgtcttca 
      ttttccaca tctaggaag gatagtgga ttgtatagc 
      aacaaacct agtaaagta gagctacca tcaatttgg 
      aatgatcct ttaaccagt ctaacttct tcaaaacag 
      agacctatt gaaaatgtt aataatata gcggccgat 
      tggctcaat aaatag
    • >TC011548 RB
      atgaatcgc tatattact cttcatcag ttaggagat 
      ggcacgtat gggtcggtg gttttggga caaagaaag 
      gatacggga gagaaggtg gcgatcaaa aggatgaaa 
      aggaaatac tactcctgg gatgaggcc atgaattta 
      agagaggtc aagtctcta aaaaaacta caccattcc 
      aatgttgtc aaactcaaa gaagtgata agagaaaat 
      gacgtcttg tactttgtt tttgagtac atgcaagaa 
      aatttgtac cagttgatc aaagatcgc cgagtgcct 
      ttaccagaa gcaacagtg aggaacatg ctttatcaa 
      attctacaa ggcctagcc ttcattcac aggcatggc 
      ttctttcac cgcgactta aaacccgaa aacatcctt 
      tgctcaggc cccgacttg atcaaaatc gccgatttt 
      ggtttagtc cgggaaatc cgctcccgt cctccttac 
      actgactac gtttcaacc cgatggtac cgcgcccct 
      gaagtgctc ctccattcc acgacctac agcagcccc 
      attgacctc tgggccgtc ggatgcata gcagccgaa 
      atatacacc ttccgaccg ttatttccc gggaccaca 
      gaaacagac caattgtac aaaatatgt gccattctt 
      ggaactcct gacaggaaa caatggccg gagtgttac 
      caactagcc ggcgctgtg gggttcaag ttcccgtat 
      ttcaccaaa acaccactt ggggatgtg gtgccccaa 
      gcaaatgcg gcagcgatt gatctaatg gacagcttc 
      ttgagctgg aatcctgcc cacagaccg actgctcaa 
      tccgcgctc aaacaccag tactttcag gtgggccaa 
      cagtacaac gtcatacac tctggagtt cgtcaaaac 
      caagccaat caaatgcaa acgcaaccc gttgtgata 
      aaaaatgaa caaatgttt ccgtccaag ccaagtgca 
      cggactact cgaaaccag tacaatcag caaatgatg 
      gcgcctccg cttcagccg tctgtgcaa atcaacatt 
      caatcgttg atcaaacca atgcaggag aagccgcaa 
      aatccgccc attgtttac agtttcggg cagtctgac 
      tttgcgaat aagtttttt cctgggaat aataaagca 
      aatacgaat aacaataat aagaaaata tcgtggggg 
      ttggatgat acaatagat tacaaagag gacgatttt 
      gcggatatt ttaggaagt aaaattaaa actcacgct 
      acggataaa tcaaaggaa aacaaaatt aacttgagc 
      caatacaat ctccaagat atcctggaa cccctagct 
      aaggcaagc gcagggact gcaaggagt caatatttg 
      agtgtttcg aggaaggat agtggattg tatagcaac 
      aaacctagt aaagtagag ctaccatca atttggaat 
      gatccttta accagtcta acttcttca aaacagaga 
      cctattgaa aatgttaat aatatagcg gccgattgg 
      ctcaataaa tag
    • >TC011548 RC
      atgaatcgc tatattact cttcatcag ttaggagat 
      ggcacgtat gggtcggtg gttttggga caaagaaag 
      gatacggga gagaaggtg gcgatcaaa aggatgaaa 
      aggaaatac tactcctgg gatgaggcc atgaattta 
      agagaggtc aagtctcta aaaaaacta caccattcc 
      aatgttgtc aaactcaaa gaagtgata agagaaaat 
      gacgtcttg tactttgtt tttgagtac atgcaagaa 
      aatttgtac cagttgatc aaagatcgc cgagtgcct 
      ttaccagaa gcaacagtg aggaacatg ctttatcaa 
      attctacaa ggcctagcc ttcattcac aggcatggc 
      ttctttcac cgcgactta aaacccgaa aacatcctt 
      tgctcaggc cccgacttg atcaaaatc gccgatttt 
      ggtttagtc cgggaaatc cgctcccgt cctccttac 
      actgactac gtttcaacc cgatggtac cgcgcccct 
      gaagtgctc ctccattcc acgacctac agcagcccc 
      attgacctc tgggccgtc ggatgcata gcagccgaa 
      atatacacc ttccgaccg ttatttccc gggaccaca 
      gaaacagac caattgtac aaaatatgt gccattctt 
      ggaactcct gacaggaaa caatggccg gagtgttac 
      caactagcc ggcgctgtg gggttcaag ttcccgtat 
      ttcaccaaa acaccactt ggggatgtg gtgccccaa 
      gcaaatgcg gcagcgatt gatctaatg gacagcttc 
      ttgagctgg aatcctgcc cacagaccg actgctcaa 
      tccgcgctc aaacaccag tactttcag gtgggccaa 
      cagtacaac gtcatacac tctggagtt cgtcaaaac 
      caagccaat caaatgcaa acgcaaccc gttgtgata 
      aaaaatgaa caaatgttt ccgtccaag ccaagtgca 
      cggactact cgaaaccag tacaatcag caaatgatg 
      gcgcctccg cttcagccg tctgtgcaa atcaacatt 
      caatcgttg atcaaacca atgcaggag aagccgcaa 
      aatccgccc attgtttac agtttcggg cagtctgac 
      tttgcgaat aagtttttt cctgggaat aataaagca 
      aatacgaat aacaataat aagaaaata tcgtggggg 
      ttggatgat acaatagat tacaaagag gacgatttt 
      gcggatatt ttaggtaaa attaaaact cacgctacg 
      gataaatca aaggaaaac aaaattaac ttgagccaa 
      tacaatctc caagatatc ctggaaccc ctagctaag 
      gcaagcgca gggactgca aggagtcaa tatttgagt 
      gtttcgagg tatgtagct ggacaaccc tctggtgtt 
      cggagagac tcaaatatc agtcttatg tcttcattt 
      tccacatct aggaaggat agtggattg tatagcaac 
      aaacctagt aaagtagag ctaccatca atttggaat 
      gatccttta accagtcta acttcttca aaacagaga 
      cctattgaa aatgttaat aatatagcg gccgattgg 
      ctcaataaa tag
    mRNA sequence
    • >TC011548 RA
      tgtgtaggg tacaacagc cgcgaatta aacatggct 
      aaaatgtta tttttgtaa ttatggact gcgtcgaaa 
      tttttcttt tatttttca tagcatgaa tcgctatat 
      tactcttca tcagttagg agatggcac gtatgggtc 
      ggtggtttt gggacaaag aaaggatac gggagagaa 
      ggtggcgat caaaaggat gaaaaggaa atactactc 
      ctgggatga ggccatgaa tttaagaga ggtcaagtc 
      tctaaaaaa actacacca ttccaatgt tgtcaaact 
      caaagaagt gataagaga aaatgacgt cttgtactt 
      tgtttttga gtacatgca agaaaattt gtaccagtt 
      gatcaaaga tcgccgagt gcctttacc agaagcaac 
      agtgaggaa catgcttta tcaaattct acaaggcct 
      agccttcat tcacaggca tggcttctt tcaccgcga 
      cttaaaacc cgaaaacat cctttgctc aggccccga 
      cttgatcaa aatcgccga ttttggttt agtccggga 
      aatccgctc ccgtcctcc ttacactga ctacgtttc 
      aacccgatg gtaccgcgc ccctgaagt gctcctcca 
      ttccacgac ctacagcag ccccattga cctctgggc 
      cgtcggatg catagcagc cgaaatata caccttccg 
      accgttatt tcccgggac cacagaaac agaccaatt 
      gtacaaaat atgtgccat tcttggaac tcctgacag 
      gaaacaatg gccggagtg ttaccaact agccggcgc 
      tgtggggtt caagttccc gtatttcac caaaacacc 
      acttgggga tgtggtgcc ccaagcaaa tgcggcagc 
      gattgatct aatggacag cttcttgag ctggaatcc 
      tgcccacag accgactgc tcaatccgc gctcaaaca 
      ccagtactt tcaggtggg ccaacagta caacgtcat 
      acactctgg agttcgtca aaaccaagc caatcaaat 
      gcaaacgca acccgttgt gataaaaaa tgaacaaat 
      gtttccgtc caagccaag tgcacggac tactcgaaa 
      ccagtacaa tcagcaaat gatggcgcc tccgcttca 
      gccgtctgt gcaaatcaa cattcaatc gttgatcaa 
      accaatgca ggagaagcc gcaaaatcc gcccattgt 
      ttacagttt cgggcagtc tgactttgc gaataagtt 
      ttttcctgg gaataataa agcaaatac gaataacaa 
      taataagaa aatatcgtg ggggttgga tgatacaat 
      agattacaa agaggacga ttttgcgga tattttagg 
      aagtaaaat taaaactca cgctacgga taaatcaaa 
      ggaaaacaa aattaactt gagccaata caatctcca 
      agatatcct ggaacccct agctaaggc aagcgcagg 
      gactgcaag gagtcaata tttgagtgt ttcgaggta 
      tgtagctgg acaaccctc tggtgttcg gagagactc 
      aaatatcag tcttatgtc ttcattttc cacatctag 
      gaaggatag tggattgta tagcaacaa acctagtaa 
      agtagagct accatcaat ttggaatga tcctttaac 
      cagtctaac ttcttcaaa acagagacc tattgaaaa 
      tgttaataa tatagcggc cgattggct caataaata 
      gctcaaacg tgtgacttg gctagatct agacggttc 
      gagacaaaa tcttagaat taaaatgtt ataagtgat 
      tggaaaaaa tcaaaagta cgcaaaatt gcatgtagc 
      attgctgaa caaacttat taatattct tcaaattgt 
      atttcatat tttttggaa tgtttcatt taaaatgtt 
      aaaataaaa tacttattt aata
    • >TC011548 RB
      tgtgtaggg tacaacagc cgcgaatta aacatggct 
      aaaatgtta tttttgtaa ttatggact gcgtcgaaa 
      tttttcttt tatttttca tagcatgaa tcgctatat 
      tactcttca tcagttagg agatggcac gtatgggtc 
      ggtggtttt gggacaaag aaaggatac gggagagaa 
      ggtggcgat caaaaggat gaaaaggaa atactactc 
      ctgggatga ggccatgaa tttaagaga ggtcaagtc 
      tctaaaaaa actacacca ttccaatgt tgtcaaact 
      caaagaagt gataagaga aaatgacgt cttgtactt 
      tgtttttga gtacatgca agaaaattt gtaccagtt 
      gatcaaaga tcgccgagt gcctttacc agaagcaac 
      agtgaggaa catgcttta tcaaattct acaaggcct 
      agccttcat tcacaggca tggcttctt tcaccgcga 
      cttaaaacc cgaaaacat cctttgctc aggccccga 
      cttgatcaa aatcgccga ttttggttt agtccggga 
      aatccgctc ccgtcctcc ttacactga ctacgtttc 
      aacccgatg gtaccgcgc ccctgaagt gctcctcca 
      ttccacgac ctacagcag ccccattga cctctgggc 
      cgtcggatg catagcagc cgaaatata caccttccg 
      accgttatt tcccgggac cacagaaac agaccaatt 
      gtacaaaat atgtgccat tcttggaac tcctgacag 
      gaaacaatg gccggagtg ttaccaact agccggcgc 
      tgtggggtt caagttccc gtatttcac caaaacacc 
      acttgggga tgtggtgcc ccaagcaaa tgcggcagc 
      gattgatct aatggacag cttcttgag ctggaatcc 
      tgcccacag accgactgc tcaatccgc gctcaaaca 
      ccagtactt tcaggtggg ccaacagta caacgtcat 
      acactctgg agttcgtca aaaccaagc caatcaaat 
      gcaaacgca acccgttgt gataaaaaa tgaacaaat 
      gtttccgtc caagccaag tgcacggac tactcgaaa 
      ccagtacaa tcagcaaat gatggcgcc tccgcttca 
      gccgtctgt gcaaatcaa cattcaatc gttgatcaa 
      accaatgca ggagaagcc gcaaaatcc gcccattgt 
      ttacagttt cgggcagtc tgactttgc gaataagtt 
      ttttcctgg gaataataa agcaaatac gaataacaa 
      taataagaa aatatcgtg ggggttgga tgatacaat 
      agattacaa agaggacga ttttgcgga tattttagg 
      aagtaaaat taaaactca cgctacgga taaatcaaa 
      ggaaaacaa aattaactt gagccaata caatctcca 
      agatatcct ggaacccct agctaaggc aagcgcagg 
      gactgcaag gagtcaata tttgagtgt ttcgaggaa 
      ggatagtgg attgtatag caacaaacc tagtaaagt 
      agagctacc atcaatttg gaatgatcc tttaaccag 
      tctaacttc ttcaaaaca gagacctat tgaaaatgt 
      taataatat agcggccga ttggctcaa taaatagct 
      caaacgtgt gacttggct agatctaga cggttcgag 
      acaaaatct tagaattaa aatgttata agtgattgg 
      aaaaaatca aaagtacgc aaaattgca tgtagcatt 
      gctgaacaa acttattaa tattcttca aattgtatt 
      tcatatttt ttggaatgt ttcatttaa aatgttaaa 
      ataaaatac ttatttaat a
    • >TC011548 RC
      tgtgtaggg tacaacagc cgcgaatta aacatggct 
      aaaatgtta tttttgtaa ttatggact gcgtcgaaa 
      tttttcttt tatttttca tagcatgaa tcgctatat 
      tactcttca tcagttagg agatggcac gtatgggtc 
      ggtggtttt gggacaaag aaaggatac gggagagaa 
      ggtggcgat caaaaggat gaaaaggaa atactactc 
      ctgggatga ggccatgaa tttaagaga ggtcaagtc 
      tctaaaaaa actacacca ttccaatgt tgtcaaact 
      caaagaagt gataagaga aaatgacgt cttgtactt 
      tgtttttga gtacatgca agaaaattt gtaccagtt 
      gatcaaaga tcgccgagt gcctttacc agaagcaac 
      agtgaggaa catgcttta tcaaattct acaaggcct 
      agccttcat tcacaggca tggcttctt tcaccgcga 
      cttaaaacc cgaaaacat cctttgctc aggccccga 
      cttgatcaa aatcgccga ttttggttt agtccggga 
      aatccgctc ccgtcctcc ttacactga ctacgtttc 
      aacccgatg gtaccgcgc ccctgaagt gctcctcca 
      ttccacgac ctacagcag ccccattga cctctgggc 
      cgtcggatg catagcagc cgaaatata caccttccg 
      accgttatt tcccgggac cacagaaac agaccaatt 
      gtacaaaat atgtgccat tcttggaac tcctgacag 
      gaaacaatg gccggagtg ttaccaact agccggcgc 
      tgtggggtt caagttccc gtatttcac caaaacacc 
      acttgggga tgtggtgcc ccaagcaaa tgcggcagc 
      gattgatct aatggacag cttcttgag ctggaatcc 
      tgcccacag accgactgc tcaatccgc gctcaaaca 
      ccagtactt tcaggtggg ccaacagta caacgtcat 
      acactctgg agttcgtca aaaccaagc caatcaaat 
      gcaaacgca acccgttgt gataaaaaa tgaacaaat 
      gtttccgtc caagccaag tgcacggac tactcgaaa 
      ccagtacaa tcagcaaat gatggcgcc tccgcttca 
      gccgtctgt gcaaatcaa cattcaatc gttgatcaa 
      accaatgca ggagaagcc gcaaaatcc gcccattgt 
      ttacagttt cgggcagtc tgactttgc gaataagtt 
      ttttcctgg gaataataa agcaaatac gaataacaa 
      taataagaa aatatcgtg ggggttgga tgatacaat 
      agattacaa agaggacga ttttgcgga tattttagg 
      taaaattaa aactcacgc tacggataa atcaaagga 
      aaacaaaat taacttgag ccaatacaa tctccaaga 
      tatcctgga acccctagc taaggcaag cgcagggac 
      tgcaaggag tcaatattt gagtgtttc gaggtatgt 
      agctggaca accctctgg tgttcggag agactcaaa 
      tatcagtct tatgtcttc attttccac atctaggaa 
      ggatagtgg attgtatag caacaaacc tagtaaagt 
      agagctacc atcaatttg gaatgatcc tttaaccag 
      tctaacttc ttcaaaaca gagacctat tgaaaatgt 
      taataatat agcggccga ttggctcaa taaatagct 
      caaacgtgt gacttggct agatctaga cggttcgag 
      acaaaatct tagaattaa aatgttata agtgattgg 
      aaaaaatca aaagtacgc aaaattgca tgtagcatt 
      gctgaacaa acttattaa tattcttca aattgtatt 
      tcatatttt ttggaatgt ttcatttaa aatgttaaa 
      ataaaatac ttatttaat a
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