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Gene information
General information
TC034580 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3756407..3767867
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034580
    iBeetle screen iB_06551

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    low validity due to technical reasons - should be repeated: musculature not scorable,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034580 RA
      MGAKPSTAN GAQSPRTRA FSTSSSSEV 
      VTAPGFRFM RSLGMDMSS DRQRARSLS 
      SVPDLHTSH ETIAIPATG ASFDVSTGA 
      SPETDSSSA EDTAGALAS AASSIALGR 
      VYAAHSLPS HIWSLNGIK CPVCSKFVI 
      PDDIECHLV MCLTKPRLS YNEDILTDD 
      KGECVICLE ELSQGDVIA RLPCLCIYH 
      KMCIDQWFE VNRSCPEHP GD
    • >TC034580 RB
      MGAKPSTAN GAQSPRTRA FSTSSSSEV 
      VTAPGFRFM RSLGMDMSS DRQRARSLS 
      SVPDLHTSH ETIAIPATG ASFDVSTGA 
      SPETDSSSA EDTAGALAS AASSIALGR 
      VYAAHSLPS HIWSLNECI TMGQNISSP 
      QPNISSVTY KDKGIKCPV CSKFVIPDD 
      IECHLVMCL TKPRLSYNE DILTDDKGE 
      CVICLEELS QGDVIARLP CLCIYHKMC 
      IDQWFEVNR SCPEHPGD
    CDS sequence
    • >TC034580 RA
      atgggtgct aaaccgagt acagcgaac ggggcgcag 
      agcccccgc acgcgggcc ttctccacg agcagcagt 
      tcggaggtg gtgacggcc ccggggttc cgcttcatg 
      aggtcgttg gggatggac atgtccagc gaccggcag 
      cgggcccgc tccctgtcc agcgtcccc gacttgcac 
      actagtcac gagaccatt gccattccg gcgacgggg 
      gccagcttc gacgtctcc acgggggcc agccccgag 
      accgacagc agctcggcc gaggacacg gcgggggcc 
      ctggcttcc gccgcctcc agcatcgcc ctggggcgc 
      gtctacgct gcacattcg ctaccctcg catatttgg 
      tcgctgaat gggatcaaa tgcccagtt tgcagcaag 
      tttgttatt cctgatgac attgaatgt cacctagtc 
      atgtgcctc actaaacca cggctttcc tacaacgaa 
      gacattttg actgacgat aaaggagag tgtgtgata 
      tgcctagaa gaattaagc caaggagat gtaatagct 
      cgtcttcct tgcctctgt atataccat aagatgtgc 
      atagatcag tggtttgaa gtaaaccgg tcgtgtccc 
      gaacaccca ggcgactag
    • >TC034580 RB
      atgggtgct aaaccgagt acagcgaac ggggcgcag 
      agcccccgc acgcgggcc ttctccacg agcagcagt 
      tcggaggtg gtgacggcc ccggggttc cgcttcatg 
      aggtcgttg gggatggac atgtccagc gaccggcag 
      cgggcccgc tccctgtcc agcgtcccc gacttgcac 
      actagtcac gagaccatt gccattccg gcgacgggg 
      gccagcttc gacgtctcc acgggggcc agccccgag 
      accgacagc agctcggcc gaggacacg gcgggggcc 
      ctggcttcc gccgcctcc agcatcgcc ctggggcgc 
      gtctacgct gcacattcg ctaccctcg catatttgg 
      tcgctgaat gagtgcatc acaatgggc caaaatatt 
      tcttcgcca cagccgaat atatcctcg gtgacatac 
      aaggacaaa gggatcaaa tgcccagtt tgcagcaag 
      tttgttatt cctgatgac attgaatgt cacctagtc 
      atgtgcctc actaaacca cggctttcc tacaacgaa 
      gacattttg actgacgat aaaggagag tgtgtgata 
      tgcctagaa gaattaagc caaggagat gtaatagct 
      cgtcttcct tgcctctgt atataccat aagatgtgc 
      atagatcag tggtttgaa gtaaaccgg tcgtgtccc 
      gaacaccca ggcgactag
    mRNA sequence
    • >TC034580 RA
      atctgttga taattgtgt gtatttcgt tcttgtcaa 
      actgtaagt gcaaaatac catgaaatt gagactaaa 
      aaattcgta ttggccatt gccaatgcg tgtggtgtt 
      tatcctgct gtcggttta aatcctgga gagtgagtg 
      tcagccccc gcgtccctc ttcgcataa acaaaggcg 
      atgtcattc gtcaaatca ggtgatttt tttgcgaga 
      aatttatga cgagtacgg agtgtttcg tcccgtaaa 
      catgtaaat agtgtggtg actggaaca ataccacaa 
      tgggtgcta aaccgagta cagcgaacg gggcgcaga 
      gcccccgca cgcgggcct tctccacga gcagcagtt 
      cggaggtgg tgacggccc cggggttcc gcttcatga 
      ggtcgttgg ggatggaca tgtccagcg accggcagc 
      gggcccgct ccctgtcca gcgtccccg acttgcaca 
      ctagtcacg agaccattg ccattccgg cgacggggg 
      ccagcttcg acgtctcca cgggggcca gccccgaga 
      ccgacagca gctcggccg aggacacgg cgggggccc 
      tggcttccg ccgcctcca gcatcgccc tggggcgcg 
      tctacgctg cacattcgc taccctcgc atatttggt 
      cgctgaatg ggatcaaat gcccagttt gcagcaagt 
      ttgttattc ctgatgaca ttgaatgtc acctagtca 
      tgtgcctca ctaaaccac ggctttcct acaacgaag 
      acattttga ctgacgata aaggagagt gtgtgatat 
      gcctagaag aattaagcc aaggagatg taatagctc 
      gtcttcctt gcctctgta tataccata agatgtgca 
      tagatcagt ggtttgaag taaaccggt cgtgtcccg 
      aacacccag gcgactagc aaaataccg aagtcagcc 
      ggggttgta aatatgtaa ataaattgc tgaagtgaa 
      ttgtgagga acaatctta attaatgca gtagctact 
      acactatag cggtcttca gacgaagat gcaacaata 
      gttacctat tctaattag ttaagtacc acactttta 
      ttacgttcg ggtcatttt ttacgactt tctacatta 
      gatcttgta ccatgatca tgccaaggc attggacaa 
      ttaatatag tgtcacata tcagttcgt ttttaatac 
      aaaagtgtt ttccgattg acgagacga ctggcagct 
      cgagaagct ttattttct ggaaaacat atttttgat 
      accttcata tgacggcta caatttatc gaatttgtt 
      tagttgtgt ttgttctaa tgcgttccg agtaataaa 
      atattcata agccgataa atacgtctt gtagacttg 
      attaagtcg ttgagatta tgataagta cgtaaggaa 
      agcggctct ttatgtttt taactgctg atacaccca 
      ttttaatta atcttccac aatgttgcg tttagaaga 
      aataatgag attgttacg ttttgtttg ttttattga 
      aaagtctgc aaagacgct ttgtatata atgaaaagt 
      taaaaatgt taaatgtgc aaatttaat gcgttcttt 
      taataaaaa taaacaaga gccttggtc atgttagtt 
      cagtaccaa aacagaccg gtagtgata taaacaacc 
      tatttttta ttctagcta acgtgaatg tgaaatcaa 
      ccaaagact ttttgttaa agtgattta atataaggt 
      gctataagt aagcaaaat actaaatta tgttaccta 
      tatgagtaa atatgtgga atttaatgg tctgggatt 
      aatttgaaa ttttatttt aattgctcg ctcgattaa 
      aaagtgcca taatttaaa aacaaattt gtgtttttt 
      ttatttaat tccattgtg tccagaccg atgaggagc 
      atctgacga gtgtgactg aatttgaat aacgtggtg 
      attttgtaa ttattttag ttatattaa tctttcatt 
      taggttatt gtaaaaata ataataaga tgtagcctt 
      gtagtgata aataataaa tgccatttt cacaa
    • >TC034580 RB
      atctgttga taattgtgt gtatttcgt tcttgtcaa 
      actgtaagt gcaaaatac catgaaatt gagactaaa 
      aaattcgta ttggccatt gccaatgcg tgtggtgtt 
      tatcctgct gtcggttta aatcctgga gagtgagtg 
      tcagccccc gcgtccctc ttcgcataa acaaaggcg 
      atgtcattc gtcaaatca ggtgatttt tttgcgaga 
      aatttatga cgagtacgg agtgtttcg tcccgtaaa 
      catgtaaat agtgtggtg actggaaca ataccacaa 
      tgggtgcta aaccgagta cagcgaacg gggcgcaga 
      gcccccgca cgcgggcct tctccacga gcagcagtt 
      cggaggtgg tgacggccc cggggttcc gcttcatga 
      ggtcgttgg ggatggaca tgtccagcg accggcagc 
      gggcccgct ccctgtcca gcgtccccg acttgcaca 
      ctagtcacg agaccattg ccattccgg cgacggggg 
      ccagcttcg acgtctcca cgggggcca gccccgaga 
      ccgacagca gctcggccg aggacacgg cgggggccc 
      tggcttccg ccgcctcca gcatcgccc tggggcgcg 
      tctacgctg cacattcgc taccctcgc atatttggt 
      cgctgaatg agtgcatca caatgggcc aaaatattt 
      cttcgccac agccgaata tatcctcgg tgacataca 
      aggacaaag ggatcaaat gcccagttt gcagcaagt 
      ttgttattc ctgatgaca ttgaatgtc acctagtca 
      tgtgcctca ctaaaccac ggctttcct acaacgaag 
      acattttga ctgacgata aaggagagt gtgtgatat 
      gcctagaag aattaagcc aaggagatg taatagctc 
      gtcttcctt gcctctgta tataccata agatgtgca 
      tagatcagt ggtttgaag taaaccggt cgtgtcccg 
      aacacccag gcgactagc aaaataccg aagtcagcc 
      ggggttgta aatatgtaa ataaattgc tgaagtgaa 
      ttgtgagga acaatctta attaatgca gtagctact 
      acactatag cggtcttca gacgaagat gcaacaata 
      gttacctat tctaattag ttaagtacc acactttta 
      ttacgttcg ggtcatttt ttacgactt tctacatta 
      gatcttgta ccatgatca tgccaaggc attggacaa 
      ttaatatag tgtcacata tcagttcgt ttttaatac 
      aaaagtgtt ttccgattg acgagacga ctggcagct 
      cgagaagct ttattttct ggaaaacat atttttgat 
      accttcata tgacggcta caatttatc gaatttgtt 
      tagttgtgt ttgttctaa tgcgttccg agtaataaa 
      atattcata agccgataa atacgtctt gtagacttg 
      attaagtcg ttgagatta tgataagta cgtaaggaa 
      agcggctct ttatgtttt taactgctg atacaccca 
      ttttaatta atcttccac aatgttgcg tttagaaga 
      aataatgag attgttacg ttttgtttg ttttattga 
      aaagtctgc aaagacgct ttgtatata atgaaaagt 
      taaaaatgt taaatgtgc aaatttaat gcgttcttt 
      taataaaaa taaacaaga gccttggtc atgttagtt 
      cagtaccaa aacagaccg gtagtgata taaacaacc 
      tatttttta ttctagcta acgtgaatg tgaaatcaa 
      ccaaagact ttttgttaa agtgattta atataaggt 
      gctataagt aagcaaaat actaaatta tgttaccta 
      tatgagtaa atatgtgga atttaatgg tctgggatt 
      aatttgaaa ttttatttt aattgctcg ctcgattaa 
      aaagtgcca taatttaaa aacaaattt gtgtttttt 
      ttatttaat tccattgtg tccagaccg atgaggagc 
      atctgacga gtgtgactg aatttgaat aacgtggtg 
      attttgtaa ttattttag ttatattaa tctttcatt 
      taggttatt gtaaaaata ataataaga tgtagcctt 
      gtagtgata aataataaa tgccatttt cacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY