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Gene information
General information
TC011543 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3735582..3736939
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011543
      iBeetle screen iB_06550

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen musculature anterior partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      Comment: larva bent
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      urogomphi size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011543 RA
        MTNVMTGIT KAQKRKNGE KKNNDVKET PTPYRYGPM 
        PKFPNQRIW SRGIVILGV LLYVWYTSK DGKEVSLAK 
        QKDILPMRG QTVDCDTKY LDEIKQFPT CVPIKCGRF 
        VSDKLVTIN EAETLLNVA KKGISLGGS SGGASILDI 
        HSGALSFGE KFVNIYTLK GSEKFITAS DLAIYKIVK 
        NKIKHAIAE NFGIDSESL YLTHPTFFS KLSNLESKT 
        PHDEYWHIH VDKETYESF HYTSLLYLT DFSNDFKGG 
        RFVFVDDAE KPMKNVTVE PRKGRVLMF TSGAENPHF 
        VERVTSGVR YAITVSFTC DKTKSISDP AAH
      CDS sequence
      • >TC011543 RA
        atgacaaac gttatgacc ggaataaca aaggcacaa 
        aaacgcaag aacggtgaa aagaaaaat aacgatgtg 
        aaagaaaca ccaacacct taccgttat ggccccatg 
        ccaaagttc ccaaatcag cgcatttgg agccgaggt 
        attgtcata ttaggagtt ctcttatac gtttggtac 
        acgtcaaag gatggcaaa gaagtgtcg ttggcgaag 
        caaaaggat attttaccc atgaggggg caaacggtg 
        gactgtgac accaaatat ttggatgaa attaaacaa 
        ttccccact tgtgttcca attaaatgc gggaggttt 
        gtctccgat aaacttgtc acaattaac gaagcagaa 
        actctgcta aatgtggcc aagaaagga atcagtcta 
        gggggctcc tctggaggc gcttcaatc ttggacatc 
        cactcaggt gccctttcg tttggggag aaatttgta 
        aatatttac accctgaaa gggtcagaa aagttcatc 
        acagcgtcg gatttagca atttataaa attgtcaaa 
        aataagatc aaacatgca attgctgaa aatttcggt 
        atagacagt gaaagtctg tacttgacg catccaact 
        tttttttcg aaattgtcc aacttggag tcaaagacg 
        ccccacgat gagtattgg cacattcat gtggacaag 
        gaaacttat gagtcattt cattacaca tcgttgttg 
        tatctcacg gatttttct aacgatttt aaaggagga 
        cgtttcgtg tttgtagac gatgccgag aaaccgatg 
        aaaaatgtt acagtagag cccaggaaa ggaagagtc 
        ttgatgttc acctcaggt gctgagaat ccccatttt 
        gtcgaacga gtgacttct ggtgttagg tacgcaatt 
        accgtttcg ttcacttgt gacaaaaca aagtcaatc 
        agtgatcca gcagcacat tag
      mRNA sequence
      • >TC011543 RA
        agatgtcaa aataaggga tgttttttt aattactcg 
        ttaatttta caatttgat gtaatttcc accaggaat 
        tggatgtaa atgacaaac gttatgacc ggaataaca 
        aaggcacaa aaacgcaag aacggtgaa aagaaaaat 
        aacgatgtg aaagaaaca ccaacacct taccgttat 
        ggccccatg ccaaagttc ccaaatcag cgcatttgg 
        agccgaggt attgtcata ttaggagtt ctcttatac 
        gtttggtac acgtcaaag gatggcaaa gaagtgtcg 
        ttggcgaag caaaaggat attttaccc atgaggggg 
        caaacggtg gactgtgac accaaatat ttggatgaa 
        attaaacaa ttccccact tgtgttcca attaaatgc 
        gggaggttt gtctccgat aaacttgtc acaattaac 
        gaagcagaa actctgcta aatgtggcc aagaaagga 
        atcagtcta gggggctcc tctggaggc gcttcaatc 
        ttggacatc cactcaggt gccctttcg tttggggag 
        aaatttgta aatatttac accctgaaa gggtcagaa 
        aagttcatc acagcgtcg gatttagca atttataaa 
        attgtcaaa aataagatc aaacatgca attgctgaa 
        aatttcggt atagacagt gaaagtctg tacttgacg 
        catccaact tttttttcg aaattgtcc aacttggag 
        tcaaagacg ccccacgat gagtattgg cacattcat 
        gtggacaag gaaacttat gagtcattt cattacaca 
        tcgttgttg tatctcacg gatttttct aacgatttt 
        aaaggagga cgtttcgtg tttgtagac gatgccgag 
        aaaccgatg aaaaatgtt acagtagag cccaggaaa 
        ggaagagtc ttgatgttc acctcaggt gctgagaat 
        ccccatttt gtcgaacga gtgacttct ggtgttagg 
        tacgcaatt accgtttcg ttcacttgt gacaaaaca 
        aagtcaatc agtgatcca gcagcacat tagctagtc 
        agtaagtaa ttagagtac ttaaacatt cctcagtat 
        aagtaatgg ttcgaataa tcgaggccg cactgtagt 
        tactgtaga taactgtta tttgccctg tatttattc 
        taaaataca gtattgttt aaagtattg tgaaagtgg 
        gtattaaaa cgtagcttt cgat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY