| General information | |||
| TC034512 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 17087..24874 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034512 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06549 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034512 RA
MADFPTELE FPVWGLLPK KETAVSSFL SKYPEYDGR GTLIGILDS GVDPGAPGL QTTSDGKVK VIHRFDCSG CGDVDTKTV VQPYENQIT TLSGRILKI PSTWNNPTN NYRLGLKHA YDLYPERLE ERMASEYKE KKWDEHHRK AVAEINRQL VEFDAKHPS PPLSDADKL IKEDLDAKL EILTNFEKK YHDAGPIYD CILFHDGEK WVCCVDTQE GDLEKCTLL GEYSITHEF APLTPGDSL NFSMNVHDN GNTLELVGL CSSHGTHVA SIAAAYFPD NPEQCGVAP GAQIASFTI GDGRLGSME TGTALVRAI IKLMELSKT QKIHVINMS YGEHAHFSD GGRIGDLMN EVVNKYGVV WVASAGNHG PALSTIGTP PDISQETII GVGAYVSPE MMVAAYSMR QKLPGMPFT WSSRGPCID GGVGVTICA PGGAVTSVP NCTLRYSQL MNGTSMASP HVAGAVSVL ISGLNQQNL PYSPYSIKR AIENTACFL TGVEVFAQG SGLLQVDKC FEALVKHHE APERNVRFH VSCGSSNSK GIYIRSKPT KSACSFNIS VEPNFLDSD NVEPDVKIK FNMKLALVC NASYVSCPT HLDVSNASR VFAIKVDPT ELAVGVHST FIEAFDVTC VAKGPVFKI PVTVVQPVE VKPPKHTVS YNSVLFKPN TIKRHFFVV PHFATWAVL RMTSTDEGS VGRFVVHSM HLLPKQSCK TLESNKAVT VTANVDSVM SFQVRSNFV LEVVIAKYW ANLGELSLD YSISFYGVK PNCQSITMH AADGIHSVE VTSLQGEEI LPAITLKNS VQILKPAEG KVSPLTARD VIPPNRQIY ELLLVYNFT LTKATEVSP NIALLSDVL YESEFESQL WLLFDSSKQ LLGCGDAYP SKYSIKLEK GDYVIRLHV RHEKKEYLD KLTEVPLLL QQKLSNAIT LDVYSSYSQ AAIAGKKSN VSHGLHSTV MPFYIAPLP ADKFVAKSN NPAHFLTGY ITYCKDDLG KKVDSHPFK YILFDSSPP KKSSNGAAT VDKSDKNKL EECREQVRD LKTQFLSKL DSLNAEKFY DEFLAKYPD HLPLHTAYT RYQSAYCKP LIRLTQGGP TRTFSYIQV TMINKDVQL KYIGVCNSA MENINEEAI LAFSAVKAD LRPDAAKIK TSMEQKRNI FLECLCRKG IILCRLYML EEKNEYLSQ INTMWKTLS RFIDTNDSK LVTPYLVYF MMWHAHVHK HYGRVMKYT LRMQEEKPS KELEERLID LCKINDWGY LGAHLQRNL LSKFPLSYR PF
- >TC034512 RA
atggccgat tttcccacc gaactcgag tttcctgtt tggggctta ttaccaaag aaggaaaca gcagttagc tcatttctc agcaaatac cccgaatat gatggcaga ggcacgctt attggaatc ctcgattcg ggagtcgat cctggagcg ccgggatta cagaccacg tccgatggc aaagttaag gttatacat aggtttgac tgcagcggt tgtggcgat gttgacacc aaaacggtc gtacaaccg tacgaaaat caaataact acgctcagt ggccgaatt ttgaagatt ccaagtacg tggaacaac cctacaaat aattacaga ctggggctc aagcacgca tatgacttg taccccgaa cggttagag gaacgcatg gcttccgaa tacaaggag aaaaagtgg gacgaacac caccggaaa gccgtagct gagatcaat agacaactg gtagagttt gacgctaaa cacccaagt ccccccctt tccgacgcc gataagctg atcaaggag gatctggac gcgaagctt gagattttg accaacttc gagaagaaa taccacgat gctgggccc atctatgac tgtattttg ttccatgat ggggagaaa tgggtgtgt tgcgtggac acgcaagaa ggggacctc gaaaagtgc acccttttg ggggagtac agcatcacg cacgagttt gcccccctg acgcccgga gacagtcta aatttcagt atgaatgtt cacgataat ggcaatacg ctagaatta gttggcctg tgttcgagt cacgggact catgttgcc tccattgcc gccgcttat ttccccgat aatcctgag cagtgtggg gttgccccc ggggctcag attgcctca tttaccatt ggtgatggg cggttgggc tccatggag acagggacg gcgctagtt agggctatt attaagctg atggagctc tcaaaaacg caaaaaatt cacgttatt aacatgagc tatggggaa catgcacac ttttctgac ggcgggcgc atcggcgac ctaatgaac gaagtggtg aacaaatac ggtgtggtg tgggtggct tccgccggc aaccacggc ccggcctta agcaccatc ggcacgccg cctgacata agccaggag acaataatc ggtgtgggg gcttacgtc tcgcccgaa atgatggtg gctgcgtac tcaatgcgc cagaaacta ccaggcatg ccgttcacc tggtcgtcc cgaggcccg tgcatagat ggcggcgtc ggcgttacg atttgcgcc ccagggggc gccgtcacg tccgtcccc aattgcacc ctccggtac tcgcagctg atgaacggg acttcgatg gcttcgccc cacgtggcg ggggctgtc agtgtgctg atttcgggg ctgaatcag cagaatttg ccctattcg ccgtacagc atcaaacgg gcgattgaa aacacggcg tgttttctg accggggtt gaggttttc gcccaggga agtgggctg ctccaagtt gataagtgc ttcgaggct ttggtgaag caccacgag gcgccggag cgaaatgtt aggtttcat gtcagctgc ggatcgtcg aattcgaaa gggatctac attcggtcg aagccgacg aagagtgct tgctcgttt aatatatca gtcgagccg aactttctt gactcggat aatgtggaa ccggacgtt aaaattaag tttaacatg aagttagcg ctggtttgt aacgcgtcg tatgtgagt tgtccgact cacttggac gtgtcgaat gctagtcga gtgtttgcg attaaagtc gacccaact gagttagcc gtcggagtg cacagcaca tttatcgaa gctttcgac gtgacgtgc gtggctaaa ggcccagtt ttcaaaatc ccggttact gtagtccag ccggttgaa gtcaaaccg cccaaacac acagtcagt tacaactcg gttctgttc aaaccgaac accatcaag agacacttt ttcgtggtg cctcacttc gccacctgg gccgttctg cggatgact tcgacggac gagggctca gtcggacgc ttcgtcgta cattccatg catttgcta ccgaaacaa agttgcaag acgttggag tcgaacaaa gcggttaca gtaaccgca aatgtcgac tcggtgatg agtttccaa gtgcggagc aattttgtc ttagaggtg gtcattgcg aagtattgg gccaacttg ggcgaactt agtttagac tattcgatt tcgttttat ggagttaag cccaattgt cagtcgatt acgatgcac gcggctgat ggcattcat tcagtcgaa gtgacgtcg ctgcaaggc gaggagatt ctacccgcg atcactttg aaaaattcc gtccagatt ttgaagcca gctgagggg aaagtctcg ccacttacg gcaagggat gtcatcccc cctaaccgg caaatctat gagcttctc ctagtgtac aattttacc ttaactaag gcaacggaa gtatcacca aatatagct ttacttagc gatgttttg tatgagtcg gagtttgag tcgcaattg tggttgttg ttcgactct agtaaacag ctcctaggc tgcggagac gcttatcca tctaagtat tcaattaaa ctagaaaaa ggcgattac gtgattcgt ctccacgtg agacacgag aaaaaggag tatttggat aaactaacc gaagttccg ttgctattg caacaaaag ttaagcaat gcgattacg cttgacgtt tacagtagc tattcacaa gctgcgatt gctggtaaa aaatcaaac gttagccat ggactgcat tccaccgtt atgcccttc tacattgct cccctacct gccgataag ttcgtagct aaatccaac aacccggct catttcctc accggttat atcacgtat tgtaaggac gacttgggg aaaaaagtc gactcgcac cctttcaaa tatatttta tttgactct tctccgccc aaaaagtcc tcgaacggc gccgccacc gtggacaaa agcgataaa aataaactg gaggagtgt cgggaacaa gttcgcgac ttgaaaacg caatttttg tccaaactg gattctttg aatgcggag aaattttat gacgagttt ttggctaaa tatccggac catttgcct ctccacacc gcctatacg cgctatcag tccgcgtat tgcaagccg ttgatccga ttgacacaa ggaggccct acccgaact tttagttat attcaagtc actatgatt aacaaagac gtgcaactt aaatacatt ggggtctgt aacagtgca atggaaaat attaacgaa gaggcgatt ttggcattt tcggccgtg aaagccgac ctgcgacct gatgctgcc aagattaaa acgtcgatg gaacaaaaa cgcaacatt ttcttggag tgtttgtgt cgtaagggt atcattctg tgtcgcttg tacatgctg gaggagaaa aacgaatat ttgtcccaa atcaacacg atgtggaag accctatcc cgctttatt gacacgaac gattctaaa ctggtgact ccttatctg gtgtatttc atgatgtgg cacgcccac gtccataag cactacggt cgtgtgatg aaatacacc ttgaggatg caagaggaa aaaccctcc aaggagctg gaggagaga ctcatcgac ttgtgcaaa ataaatgac tgggggtat ttaggggct cacttgcag agaaaccta ctttcgaaa ttcccccta tcgtaccga ccgttttaa
- >TC034512 RA
aacagtgac gtacgtgga ggcagtgtt aaacaatgg ccgattttc ccaccgaac tcgagtttc ctgtttggg gcttattac caaagaagg aaacagcag ttagctcat ttctcagca aataccccg aatatgatg gcagaggca cgcttattg gaatcctcg attcgggag tcgatcctg gagcgccgg gattacaga ccacgtccg atggcaaag ttaaggtta tacataggt ttgactgca gcggttgtg gcgatgttg acaccaaaa cggtcgtac aaccgtacg aaaatcaaa taactacgc tcagtggcc gaattttga agattccaa gtacgtgga acaacccta caaataatt acagactgg ggctcaagc acgcatatg acttgtacc ccgaacggt tagaggaac gcatggctt ccgaataca aggagaaaa agtgggacg aacaccacc ggaaagccg tagctgaga tcaatagac aactggtag agtttgacg ctaaacacc caagtcccc ccctttccg acgccgata agctgatca aggaggatc tggacgcga agcttgaga ttttgacca acttcgaga agaaatacc acgatgctg ggcccatct atgactgta ttttgttcc atgatgggg agaaatggg tgtgttgcg tggacacgc aagaagggg acctcgaaa agtgcaccc ttttggggg agtacagca tcacgcacg agtttgccc ccctgacgc ccggagaca gtctaaatt tcagtatga atgttcacg ataatggca atacgctag aattagttg gcctgtgtt cgagtcacg ggactcatg ttgcctcca ttgccgccg cttatttcc ccgataatc ctgagcagt gtggggttg cccccgggg ctcagattg cctcattta ccattggtg atgggcggt tgggctcca tggagacag ggacggcgc tagttaggg ctattatta agctgatgg agctctcaa aaacgcaaa aaattcacg ttattaaca tgagctatg gggaacatg cacactttt ctgacggcg ggcgcatcg gcgacctaa tgaacgaag tggtgaaca aatacggtg tggtgtggg tggcttccg ccggcaacc acggcccgg ccttaagca ccatcggca cgccgcctg acataagcc aggagacaa taatcggtg tgggggctt acgtctcgc ccgaaatga tggtggctg cgtactcaa tgcgccaga aactaccag gcatgccgt tcacctggt cgtcccgag gcccgtgca tagatggcg gcgtcggcg ttacgattt gcgccccag ggggcgccg tcacgtccg tccccaatt gcaccctcc ggtactcgc agctgatga acgggactt cgatggctt cgccccacg tggcggggg ctgtcagtg tgctgattt cggggctga atcagcaga atttgccct attcgccgt acagcatca aacgggcga ttgaaaaca cggcgtgtt ttctgaccg gggttgagg ttttcgccc agggaagtg ggctgctcc aagttgata agtgcttcg aggctttgg tgaagcacc acgaggcgc cggagcgaa atgttaggt ttcatgtca gctgcggat cgtcgaatt cgaaaggga tctacattc ggtcgaagc cgacgaaga gtgcttgct cgtttaata tatcagtcg agccgaact ttcttgact cggataatg tggaaccgg acgttaaaa ttaagttta acatgaagt tagcgctgg tttgtaacg cgtcgtatg tgagttgtc cgactcact tggacgtgt cgaatgcta gtcgagtgt ttgcgatta aagtcgacc caactgagt tagccgtcg gagtgcaca gcacattta tcgaagctt tcgacgtga cgtgcgtgg ctaaaggcc cagttttca aaatcccgg ttactgtag tccagccgg ttgaagtca aaccgccca aacacacag tcagttaca actcggttc tgttcaaac cgaacacca tcaagagac actttttcg tggtgcctc acttcgcca cctgggccg ttctgcgga tgacttcga cggacgagg gctcagtcg gacgcttcg tcgtacatt ccatgcatt tgctaccga aacaaagtt gcaagacgt tggagtcga acaaagcgg ttacagtaa ccgcaaatg tcgactcgg tgatgagtt tccaagtgc ggagcaatt ttgtcttag aggtggtca ttgcgaagt attgggcca acttgggcg aacttagtt tagactatt cgatttcgt tttatggag ttaagccca attgtcagt cgattacga tgcacgcgg ctgatggca ttcattcag tcgaagtga cgtcgctgc aaggcgagg agattctac ccgcgatca ctttgaaaa attccgtcc agattttga agccagctg aggggaaag tctcgccac ttacggcaa gggatgtca tccccccta accggcaaa tctatgagc ttctcctag tgtacaatt ttaccttaa ctaaggcaa cggaagtat caccaaata tagctttac ttagcgatg ttttgtatg agtcggagt ttgagtcgc aattgtggt tgttgttcg actctagta aacagctcc taggctgcg gagacgctt atccatcta agtattcaa ttaaactag aaaaaggcg attacgtga ttcgtctcc acgtgagac acgagaaaa aggagtatt tggataaac taaccgaag ttccgttgc tattgcaac aaaagttaa gcaatgcga ttacgcttg acgtttaca gtagctatt cacaagctg cgattgctg gtaaaaaat caaacgtta gccatggac tgcattcca ccgttatgc ccttctaca ttgctcccc tacctgccg ataagttcg tagctaaat ccaacaacc cggctcatt tcctcaccg gttatatca cgtattgta aggacgact tggggaaaa aagtcgact cgcaccctt tcaaatata ttttatttg actcttctc cgcccaaaa agtcctcga acggcgccg ccaccgtgg acaaaagcg ataaaaata aactggagg agtgtcggg aacaagttc gcgacttga aaacgcaat ttttgtcca aactggatt ctttgaatg cggagaaat tttatgacg agtttttgg ctaaatatc cggaccatt tgcctctcc acaccgcct atacgcgct atcagtccg cgtattgca agccgttga tccgattga cacaaggag gccctaccc gaactttta gttatattc aagtcacta tgattaaca aagacgtgc aacttaaat acattgggg tctgtaaca gtgcaatgg aaaatatta acgaagagg cgattttgg cattttcgg ccgtgaaag ccgacctgc gacctgatg ctgccaaga ttaaaacgt cgatggaac aaaaacgca acattttct tggagtgtt tgtgtcgta agggtatca ttctgtgtc gcttgtaca tgctggagg agaaaaacg aatatttgt cccaaatca acacgatgt ggaagaccc tatcccgct ttattgaca cgaacgatt ctaaactgg tgactcctt atctggtgt atttcatga tgtggcacg cccacgtcc ataagcact acggtcgtg tgatgaaat acaccttga ggatgcaag aggaaaaac cctccaagg agctggagg agagactca tcgacttgt gcaaaataa atgactggg ggtatttag gggctcact tgcagagaa acctacttt cgaaattcc ccctatcgt accgaccgt tttaaaact agttaatgt tttgttcgc tttgtttta cgggtagtt taataatcg ccaaattgc tttttcatg ttttctcgt tacctgtgc tgtttgaat gaat
