Status
Please wait ...
Gene information
General information
TC034512 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 17087..24874
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034512
    iBeetle screen iB_06549

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034512 RA
      MADFPTELE FPVWGLLPK KETAVSSFL SKYPEYDGR 
      GTLIGILDS GVDPGAPGL QTTSDGKVK VIHRFDCSG 
      CGDVDTKTV VQPYENQIT TLSGRILKI PSTWNNPTN 
      NYRLGLKHA YDLYPERLE ERMASEYKE KKWDEHHRK 
      AVAEINRQL VEFDAKHPS PPLSDADKL IKEDLDAKL 
      EILTNFEKK YHDAGPIYD CILFHDGEK WVCCVDTQE 
      GDLEKCTLL GEYSITHEF APLTPGDSL NFSMNVHDN 
      GNTLELVGL CSSHGTHVA SIAAAYFPD NPEQCGVAP 
      GAQIASFTI GDGRLGSME TGTALVRAI IKLMELSKT 
      QKIHVINMS YGEHAHFSD GGRIGDLMN EVVNKYGVV 
      WVASAGNHG PALSTIGTP PDISQETII GVGAYVSPE 
      MMVAAYSMR QKLPGMPFT WSSRGPCID GGVGVTICA 
      PGGAVTSVP NCTLRYSQL MNGTSMASP HVAGAVSVL 
      ISGLNQQNL PYSPYSIKR AIENTACFL TGVEVFAQG 
      SGLLQVDKC FEALVKHHE APERNVRFH VSCGSSNSK 
      GIYIRSKPT KSACSFNIS VEPNFLDSD NVEPDVKIK 
      FNMKLALVC NASYVSCPT HLDVSNASR VFAIKVDPT 
      ELAVGVHST FIEAFDVTC VAKGPVFKI PVTVVQPVE 
      VKPPKHTVS YNSVLFKPN TIKRHFFVV PHFATWAVL 
      RMTSTDEGS VGRFVVHSM HLLPKQSCK TLESNKAVT 
      VTANVDSVM SFQVRSNFV LEVVIAKYW ANLGELSLD 
      YSISFYGVK PNCQSITMH AADGIHSVE VTSLQGEEI 
      LPAITLKNS VQILKPAEG KVSPLTARD VIPPNRQIY 
      ELLLVYNFT LTKATEVSP NIALLSDVL YESEFESQL 
      WLLFDSSKQ LLGCGDAYP SKYSIKLEK GDYVIRLHV 
      RHEKKEYLD KLTEVPLLL QQKLSNAIT LDVYSSYSQ 
      AAIAGKKSN VSHGLHSTV MPFYIAPLP ADKFVAKSN 
      NPAHFLTGY ITYCKDDLG KKVDSHPFK YILFDSSPP 
      KKSSNGAAT VDKSDKNKL EECREQVRD LKTQFLSKL 
      DSLNAEKFY DEFLAKYPD HLPLHTAYT RYQSAYCKP 
      LIRLTQGGP TRTFSYIQV TMINKDVQL KYIGVCNSA 
      MENINEEAI LAFSAVKAD LRPDAAKIK TSMEQKRNI 
      FLECLCRKG IILCRLYML EEKNEYLSQ INTMWKTLS 
      RFIDTNDSK LVTPYLVYF MMWHAHVHK HYGRVMKYT 
      LRMQEEKPS KELEERLID LCKINDWGY LGAHLQRNL 
      LSKFPLSYR PF
    CDS sequence
    • >TC034512 RA
      atggccgat tttcccacc gaactcgag tttcctgtt 
      tggggctta ttaccaaag aaggaaaca gcagttagc 
      tcatttctc agcaaatac cccgaatat gatggcaga 
      ggcacgctt attggaatc ctcgattcg ggagtcgat 
      cctggagcg ccgggatta cagaccacg tccgatggc 
      aaagttaag gttatacat aggtttgac tgcagcggt 
      tgtggcgat gttgacacc aaaacggtc gtacaaccg 
      tacgaaaat caaataact acgctcagt ggccgaatt 
      ttgaagatt ccaagtacg tggaacaac cctacaaat 
      aattacaga ctggggctc aagcacgca tatgacttg 
      taccccgaa cggttagag gaacgcatg gcttccgaa 
      tacaaggag aaaaagtgg gacgaacac caccggaaa 
      gccgtagct gagatcaat agacaactg gtagagttt 
      gacgctaaa cacccaagt ccccccctt tccgacgcc 
      gataagctg atcaaggag gatctggac gcgaagctt 
      gagattttg accaacttc gagaagaaa taccacgat 
      gctgggccc atctatgac tgtattttg ttccatgat 
      ggggagaaa tgggtgtgt tgcgtggac acgcaagaa 
      ggggacctc gaaaagtgc acccttttg ggggagtac 
      agcatcacg cacgagttt gcccccctg acgcccgga 
      gacagtcta aatttcagt atgaatgtt cacgataat 
      ggcaatacg ctagaatta gttggcctg tgttcgagt 
      cacgggact catgttgcc tccattgcc gccgcttat 
      ttccccgat aatcctgag cagtgtggg gttgccccc 
      ggggctcag attgcctca tttaccatt ggtgatggg 
      cggttgggc tccatggag acagggacg gcgctagtt 
      agggctatt attaagctg atggagctc tcaaaaacg 
      caaaaaatt cacgttatt aacatgagc tatggggaa 
      catgcacac ttttctgac ggcgggcgc atcggcgac 
      ctaatgaac gaagtggtg aacaaatac ggtgtggtg 
      tgggtggct tccgccggc aaccacggc ccggcctta 
      agcaccatc ggcacgccg cctgacata agccaggag 
      acaataatc ggtgtgggg gcttacgtc tcgcccgaa 
      atgatggtg gctgcgtac tcaatgcgc cagaaacta 
      ccaggcatg ccgttcacc tggtcgtcc cgaggcccg 
      tgcatagat ggcggcgtc ggcgttacg atttgcgcc 
      ccagggggc gccgtcacg tccgtcccc aattgcacc 
      ctccggtac tcgcagctg atgaacggg acttcgatg 
      gcttcgccc cacgtggcg ggggctgtc agtgtgctg 
      atttcgggg ctgaatcag cagaatttg ccctattcg 
      ccgtacagc atcaaacgg gcgattgaa aacacggcg 
      tgttttctg accggggtt gaggttttc gcccaggga 
      agtgggctg ctccaagtt gataagtgc ttcgaggct 
      ttggtgaag caccacgag gcgccggag cgaaatgtt 
      aggtttcat gtcagctgc ggatcgtcg aattcgaaa 
      gggatctac attcggtcg aagccgacg aagagtgct 
      tgctcgttt aatatatca gtcgagccg aactttctt 
      gactcggat aatgtggaa ccggacgtt aaaattaag 
      tttaacatg aagttagcg ctggtttgt aacgcgtcg 
      tatgtgagt tgtccgact cacttggac gtgtcgaat 
      gctagtcga gtgtttgcg attaaagtc gacccaact 
      gagttagcc gtcggagtg cacagcaca tttatcgaa 
      gctttcgac gtgacgtgc gtggctaaa ggcccagtt 
      ttcaaaatc ccggttact gtagtccag ccggttgaa 
      gtcaaaccg cccaaacac acagtcagt tacaactcg 
      gttctgttc aaaccgaac accatcaag agacacttt 
      ttcgtggtg cctcacttc gccacctgg gccgttctg 
      cggatgact tcgacggac gagggctca gtcggacgc 
      ttcgtcgta cattccatg catttgcta ccgaaacaa 
      agttgcaag acgttggag tcgaacaaa gcggttaca 
      gtaaccgca aatgtcgac tcggtgatg agtttccaa 
      gtgcggagc aattttgtc ttagaggtg gtcattgcg 
      aagtattgg gccaacttg ggcgaactt agtttagac 
      tattcgatt tcgttttat ggagttaag cccaattgt 
      cagtcgatt acgatgcac gcggctgat ggcattcat 
      tcagtcgaa gtgacgtcg ctgcaaggc gaggagatt 
      ctacccgcg atcactttg aaaaattcc gtccagatt 
      ttgaagcca gctgagggg aaagtctcg ccacttacg 
      gcaagggat gtcatcccc cctaaccgg caaatctat 
      gagcttctc ctagtgtac aattttacc ttaactaag 
      gcaacggaa gtatcacca aatatagct ttacttagc 
      gatgttttg tatgagtcg gagtttgag tcgcaattg 
      tggttgttg ttcgactct agtaaacag ctcctaggc 
      tgcggagac gcttatcca tctaagtat tcaattaaa 
      ctagaaaaa ggcgattac gtgattcgt ctccacgtg 
      agacacgag aaaaaggag tatttggat aaactaacc 
      gaagttccg ttgctattg caacaaaag ttaagcaat 
      gcgattacg cttgacgtt tacagtagc tattcacaa 
      gctgcgatt gctggtaaa aaatcaaac gttagccat 
      ggactgcat tccaccgtt atgcccttc tacattgct 
      cccctacct gccgataag ttcgtagct aaatccaac 
      aacccggct catttcctc accggttat atcacgtat 
      tgtaaggac gacttgggg aaaaaagtc gactcgcac 
      cctttcaaa tatatttta tttgactct tctccgccc 
      aaaaagtcc tcgaacggc gccgccacc gtggacaaa 
      agcgataaa aataaactg gaggagtgt cgggaacaa 
      gttcgcgac ttgaaaacg caatttttg tccaaactg 
      gattctttg aatgcggag aaattttat gacgagttt 
      ttggctaaa tatccggac catttgcct ctccacacc 
      gcctatacg cgctatcag tccgcgtat tgcaagccg 
      ttgatccga ttgacacaa ggaggccct acccgaact 
      tttagttat attcaagtc actatgatt aacaaagac 
      gtgcaactt aaatacatt ggggtctgt aacagtgca 
      atggaaaat attaacgaa gaggcgatt ttggcattt 
      tcggccgtg aaagccgac ctgcgacct gatgctgcc 
      aagattaaa acgtcgatg gaacaaaaa cgcaacatt 
      ttcttggag tgtttgtgt cgtaagggt atcattctg 
      tgtcgcttg tacatgctg gaggagaaa aacgaatat 
      ttgtcccaa atcaacacg atgtggaag accctatcc 
      cgctttatt gacacgaac gattctaaa ctggtgact 
      ccttatctg gtgtatttc atgatgtgg cacgcccac 
      gtccataag cactacggt cgtgtgatg aaatacacc 
      ttgaggatg caagaggaa aaaccctcc aaggagctg 
      gaggagaga ctcatcgac ttgtgcaaa ataaatgac 
      tgggggtat ttaggggct cacttgcag agaaaccta 
      ctttcgaaa ttcccccta tcgtaccga ccgttttaa
    mRNA sequence
    • >TC034512 RA
      aacagtgac gtacgtgga ggcagtgtt aaacaatgg 
      ccgattttc ccaccgaac tcgagtttc ctgtttggg 
      gcttattac caaagaagg aaacagcag ttagctcat 
      ttctcagca aataccccg aatatgatg gcagaggca 
      cgcttattg gaatcctcg attcgggag tcgatcctg 
      gagcgccgg gattacaga ccacgtccg atggcaaag 
      ttaaggtta tacataggt ttgactgca gcggttgtg 
      gcgatgttg acaccaaaa cggtcgtac aaccgtacg 
      aaaatcaaa taactacgc tcagtggcc gaattttga 
      agattccaa gtacgtgga acaacccta caaataatt 
      acagactgg ggctcaagc acgcatatg acttgtacc 
      ccgaacggt tagaggaac gcatggctt ccgaataca 
      aggagaaaa agtgggacg aacaccacc ggaaagccg 
      tagctgaga tcaatagac aactggtag agtttgacg 
      ctaaacacc caagtcccc ccctttccg acgccgata 
      agctgatca aggaggatc tggacgcga agcttgaga 
      ttttgacca acttcgaga agaaatacc acgatgctg 
      ggcccatct atgactgta ttttgttcc atgatgggg 
      agaaatggg tgtgttgcg tggacacgc aagaagggg 
      acctcgaaa agtgcaccc ttttggggg agtacagca 
      tcacgcacg agtttgccc ccctgacgc ccggagaca 
      gtctaaatt tcagtatga atgttcacg ataatggca 
      atacgctag aattagttg gcctgtgtt cgagtcacg 
      ggactcatg ttgcctcca ttgccgccg cttatttcc 
      ccgataatc ctgagcagt gtggggttg cccccgggg 
      ctcagattg cctcattta ccattggtg atgggcggt 
      tgggctcca tggagacag ggacggcgc tagttaggg 
      ctattatta agctgatgg agctctcaa aaacgcaaa 
      aaattcacg ttattaaca tgagctatg gggaacatg 
      cacactttt ctgacggcg ggcgcatcg gcgacctaa 
      tgaacgaag tggtgaaca aatacggtg tggtgtggg 
      tggcttccg ccggcaacc acggcccgg ccttaagca 
      ccatcggca cgccgcctg acataagcc aggagacaa 
      taatcggtg tgggggctt acgtctcgc ccgaaatga 
      tggtggctg cgtactcaa tgcgccaga aactaccag 
      gcatgccgt tcacctggt cgtcccgag gcccgtgca 
      tagatggcg gcgtcggcg ttacgattt gcgccccag 
      ggggcgccg tcacgtccg tccccaatt gcaccctcc 
      ggtactcgc agctgatga acgggactt cgatggctt 
      cgccccacg tggcggggg ctgtcagtg tgctgattt 
      cggggctga atcagcaga atttgccct attcgccgt 
      acagcatca aacgggcga ttgaaaaca cggcgtgtt 
      ttctgaccg gggttgagg ttttcgccc agggaagtg 
      ggctgctcc aagttgata agtgcttcg aggctttgg 
      tgaagcacc acgaggcgc cggagcgaa atgttaggt 
      ttcatgtca gctgcggat cgtcgaatt cgaaaggga 
      tctacattc ggtcgaagc cgacgaaga gtgcttgct 
      cgtttaata tatcagtcg agccgaact ttcttgact 
      cggataatg tggaaccgg acgttaaaa ttaagttta 
      acatgaagt tagcgctgg tttgtaacg cgtcgtatg 
      tgagttgtc cgactcact tggacgtgt cgaatgcta 
      gtcgagtgt ttgcgatta aagtcgacc caactgagt 
      tagccgtcg gagtgcaca gcacattta tcgaagctt 
      tcgacgtga cgtgcgtgg ctaaaggcc cagttttca 
      aaatcccgg ttactgtag tccagccgg ttgaagtca 
      aaccgccca aacacacag tcagttaca actcggttc 
      tgttcaaac cgaacacca tcaagagac actttttcg 
      tggtgcctc acttcgcca cctgggccg ttctgcgga 
      tgacttcga cggacgagg gctcagtcg gacgcttcg 
      tcgtacatt ccatgcatt tgctaccga aacaaagtt 
      gcaagacgt tggagtcga acaaagcgg ttacagtaa 
      ccgcaaatg tcgactcgg tgatgagtt tccaagtgc 
      ggagcaatt ttgtcttag aggtggtca ttgcgaagt 
      attgggcca acttgggcg aacttagtt tagactatt 
      cgatttcgt tttatggag ttaagccca attgtcagt 
      cgattacga tgcacgcgg ctgatggca ttcattcag 
      tcgaagtga cgtcgctgc aaggcgagg agattctac 
      ccgcgatca ctttgaaaa attccgtcc agattttga 
      agccagctg aggggaaag tctcgccac ttacggcaa 
      gggatgtca tccccccta accggcaaa tctatgagc 
      ttctcctag tgtacaatt ttaccttaa ctaaggcaa 
      cggaagtat caccaaata tagctttac ttagcgatg 
      ttttgtatg agtcggagt ttgagtcgc aattgtggt 
      tgttgttcg actctagta aacagctcc taggctgcg 
      gagacgctt atccatcta agtattcaa ttaaactag 
      aaaaaggcg attacgtga ttcgtctcc acgtgagac 
      acgagaaaa aggagtatt tggataaac taaccgaag 
      ttccgttgc tattgcaac aaaagttaa gcaatgcga 
      ttacgcttg acgtttaca gtagctatt cacaagctg 
      cgattgctg gtaaaaaat caaacgtta gccatggac 
      tgcattcca ccgttatgc ccttctaca ttgctcccc 
      tacctgccg ataagttcg tagctaaat ccaacaacc 
      cggctcatt tcctcaccg gttatatca cgtattgta 
      aggacgact tggggaaaa aagtcgact cgcaccctt 
      tcaaatata ttttatttg actcttctc cgcccaaaa 
      agtcctcga acggcgccg ccaccgtgg acaaaagcg 
      ataaaaata aactggagg agtgtcggg aacaagttc 
      gcgacttga aaacgcaat ttttgtcca aactggatt 
      ctttgaatg cggagaaat tttatgacg agtttttgg 
      ctaaatatc cggaccatt tgcctctcc acaccgcct 
      atacgcgct atcagtccg cgtattgca agccgttga 
      tccgattga cacaaggag gccctaccc gaactttta 
      gttatattc aagtcacta tgattaaca aagacgtgc 
      aacttaaat acattgggg tctgtaaca gtgcaatgg 
      aaaatatta acgaagagg cgattttgg cattttcgg 
      ccgtgaaag ccgacctgc gacctgatg ctgccaaga 
      ttaaaacgt cgatggaac aaaaacgca acattttct 
      tggagtgtt tgtgtcgta agggtatca ttctgtgtc 
      gcttgtaca tgctggagg agaaaaacg aatatttgt 
      cccaaatca acacgatgt ggaagaccc tatcccgct 
      ttattgaca cgaacgatt ctaaactgg tgactcctt 
      atctggtgt atttcatga tgtggcacg cccacgtcc 
      ataagcact acggtcgtg tgatgaaat acaccttga 
      ggatgcaag aggaaaaac cctccaagg agctggagg 
      agagactca tcgacttgt gcaaaataa atgactggg 
      ggtatttag gggctcact tgcagagaa acctacttt 
      cgaaattcc ccctatcgt accgaccgt tttaaaact 
      agttaatgt tttgttcgc tttgtttta cgggtagtt 
      taataatcg ccaaattgc tttttcatg ttttctcgt 
      tacctgtgc tgtttgaat gaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY