Status
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Gene information
General information
TC004697 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 74583..84489
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004697
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06546

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004697 RA
      MDMGVRKLP KKRKFDPSE LEENTTASY IPVSVVQSA 
      PQATAVDYS CPTNKQNTV DLSEWCDHR VLAKQGDWY 
      YPGVIREAR GSNITVELD GKEEKLVHF TNVFDNECY 
      NVIGDASPS KNEVTLGAR VCVRQNQQQ MFVEGVVFN 
      ICNEGQVVR FVVAVIGEK QLKITVKRS ELRLLRPPW 
      YDELVEPLI QESITPKLE YFANTTVSP HTPVSACTP 
      QSNGRNYDD YCESEEDEL RRGDITFNS EVDAKLSGS 
      SKRSSMHSR GSSSSSITP RSQPTTPRS QATTPHKYK 
      KGDVVSNPN GIRKKFNGK QWRRLCSKD GCTKESQRR 
      GYCSRHLSL KGNSLRSGP AFPRSNSKA DGEDTSRDS 
      ETSPNCNDR RITGRFDQE ETDAANMLV SLGSSRSAT 
      PAYSPNHQG SSPHTMQSP ITVGSRQNV FMPIISPGL 
      QKINSPGPQ GYNTAYHQP LPLRPESVR PIQGVPSVI 
      RLTSSPRQW GPNSTDHQQ GVILQQALT TNPPVETEN 
      TQIQSGTVL MHSTNASLL PVIVNPTQL LPVLPSASQ 
      SVVKKVIPS GTVPQPTPP VIVKTEQVA SNLQQNHKD 
      PVIHHAPQP RISVQSHTA TSPQFTQTP PRPVAPQPK 
      ISTNSQSAF VIPWHSIVP ILTACPESN SPPPSELSP 
      PLSAPPVPI SATKMDVPD DDAEELIPI PAEEDDDVF 
      ENEPTDASL NENSTSKRR TQSLSSLQT PKDNNLVKN 
      KDRIRRPMN AFMIFSKRH RALVHQRHP NQDNRTVSK 
      ILGEWWYAL GPEQKRKYH ELASEVKEA HFKAHPEWK 
      WCNKDRRKS STGSTRSKL SSTGDSAEV GEVPMSPQP 
      LNSPAPLAE PPRQILPDN PGDVSDDDQ MVIIEEPGT 
      VEIDLKCKE KVTDSDSES QSDLEGGEN RFSQQRFSP 
      VSKTGTDVT YRPKPIKAI MPTSDSSTK YSPVASSSS 
      LGFHYSPVN PSGVTGFQP TGGAFKQAP QAKSVIQIG 
      DSHQNSQFG SQQPVTVAI FTGQSVCLS NDTERSQPV 
      VVVASTPSE QPVQYVYMP PPFTVSDSN GRNLSVPVQ 
      LVSKTSSQA DGGKKEFKL APTPAQLGK APLQRRQSM 
      APTTSNNTA QVTSEPSVP LPPPPTSES STQDCLVSP 
      IPKKSLFKR NKEDGMDKV LDQVNFEKK FCSLPQFKP 
      EECQSPSAI SSSSPGLFA YKKRNLSLT NRSSVDEES 
      ESETPQSVP KSSSSLKPA VFFGPDFNV DNVREMNEM 
      GEGSSPRTP RTPGTSKES EKGHRKILE QRRQLVMQL 
      FHEHTYFPS AQATSNFQS QHSDIFPSK ASLQLKIRE 
      VRQKVMARN NSTPHSANS QSSPVTPAE PLNVSSSS
    CDS sequence
    • >TC004697 RA
      atggatatg ggtgtccga aaactgccg aaaaaacga 
      aaatttgac ccctccgaa ctggaggaa aacacgact 
      gcttcgtac attcctgtg tctgttgtt caaagtgca 
      ccgcaagcc acagccgtc gattactcc tgccctacc 
      aataagcaa aacaccgtc gatctcagc gagtggtgc 
      gaccacagg gtgctcgcc aagcagggc gactggtac 
      taccctggg gtcatcagg gaggcccga ggaagcaat 
      ataaccgtg gagctggac ggcaaggag gagaagctg 
      gtgcacttc accaacgtg tttgataac gagtgttac 
      aacgtgata ggggacgcg agtccgtcc aagaacgag 
      gtgacgctc ggggcgcgc gtttgcgtc cgtcagaac 
      cagcaacaa atgttcgtg gagggtgtg gtatttaac 
      atttgtaac gagggacaa gtcgtgcgt ttcgtggtg 
      gccgtaatc ggcgaaaaa cagttgaaa ataacggtt 
      aaacggtca gaactgcgc cttttgcgc cctccatgg 
      tacgatgag ctcgtggag cctttgatc caggagtcc 
      ataacgccg aaattggag tactttgca aacacgacg 
      gtgtcgccc cacacaccg gtctccgca tgcactccc 
      caatcgaac ggtcgcaac tatgacgac tactgcgag 
      agcgaagaa gacgagttg cgaagaggt gatattacg 
      tttaattcc gaggtagac gctaagctt tcagggagt 
      tcgaagcgg agtagtatg catagtaga ggaagctcc 
      tccagcagt ataactccc cgatctcag ccgaccact 
      ccgcggagc caagccacg acccctcac aagtataag 
      aaaggcgac gtggtgtcg aacccaaac ggcatacgc 
      aaaaagttc aacgggaaa cagtggagg aggttgtgt 
      tcgaaagac ggttgtacg aaagaatct caaaggcgg 
      gggtactgt tcaaggcat cttagcctc aaaggtaat 
      agccttcga agtggtccc gcgtttccg cgttctaat 
      agcaaagca gatggtgag gatacttcc agagattcg 
      gaaacttcg ccgaattgt aacgatcga aggatcacc 
      ggacggttc gaccaagag gaaactgac gcggccaat 
      atgttagtt tctttaggt agttcgcga tcggccact 
      ccggcctat tcgcccaat caccaaggc tcatcgcct 
      catacaatg caatcaccg atcacagtc gggtcccga 
      caaaacgtt ttcatgcct attatcagt ccaggcttg 
      caaaaaatc aactcgccg gggccacaa ggatacaac 
      acggcatat caccaaccc ctaccttta agacccgag 
      tcagttcgg ccgatccag ggcgtgcca agtgtcatt 
      cgactgacc tccagtcct cgtcagtgg ggtcctaac 
      tcgaccgac caccaacaa ggcgtgatt ctgcagcag 
      gctttgacg acaaaccca cctgtagaa accgaaaac 
      acccaaatc caatcagga actgttcta atgcattca 
      actaatgca agtctacta ccagttata gtcaatcca 
      actcagctg ttacctgtt ttgccttct gcatcacag 
      tccgttgta aaaaaggtg atccctagc ggcaccgtg 
      ccccagccc acgccgcca gtgatcgtc aaaacggag 
      caagttgcg tcgaatttg caacagaac cacaaggac 
      ccagtgatc caccacgcg ccgcagccg cgcatttcc 
      gttcagtcg cacaccgcg acctcgccc cagttcacc 
      caaacgcct ccgcggcct gtggccccc cagccgaag 
      atctcgacg aattcccaa tcagccttc gtaatcccc 
      tggcactcg atcgttccg attttgacc gcttgtcct 
      gaatccaac tcgccgccc ccttccgag ctctcgccc 
      ccgttatca gcgccccct gttcccatc tcagcgacg 
      aaaatggac gtacctgat gacgacgcc gaagaactg 
      attccaatt ccggccgaa gaagacgac gatgttttc 
      gaaaatgaa ccaacggat gcgagtttg aacgaaaat 
      tcgacgagc aaacggcgg acgcagtca ctgagctcg 
      ctacagacc ccgaaggat aataatttg gtcaagaat 
      aaggaccgg attcggcgg cctatgaat gctttcatg 
      attttttcg aaacgacat cgggctttg gtccatcag 
      aggcacccc aatcaggac aacaggacc gtatcgaag 
      attttgggc gagtggtgg tacgcccta ggcccggaa 
      cagaagcgg aagtatcac gagttggcg tcggaggtc 
      aaggaggca cacttcaag gcgcatccg gagtggaaa 
      tggtgcaac aaggaccgc cggaaatcg tcaaccggt 
      tcaactcgc agcaagttg agttcgacc ggtgacagt 
      gccgaagtg ggcgaagtg ccgatgagc ccccagccg 
      ttgaactct ccggcgcct ttggcggag ccccctcgc 
      caaatcctc ccagacaac cctggggac gtctccgac 
      gacgaccag atggtcata atcgaggag ccgggaacc 
      gtcgaaatc gacttgaaa tgcaaagaa aaagtaacc 
      gattcggac tctgaatcg cagagcgat ttggaagga 
      ggcgagaac cgcttctcg cagcagcgt ttcagtccc 
      gtgtccaaa accggcacg gacgtcacc tacaggcct 
      aaaccgatc aaagcaata atgccaacc tcggacagc 
      tcgaccaag tacagcccg gttgcgagt tcgagtagt 
      ttgggtttt cactactcg ccggtgaat ccctcggga 
      gttaccggt ttccagccc acagggggc gctttcaag 
      caagcaccg caggccaaa tcagtgatt cagatcggc 
      gatagtcat cagaatagt caatttggg agtcaacaa 
      ccggtcaca gttgcgatt tttacggga cagtcggtt 
      tgtttgtcg aatgatacg gagcgttcg caaccggtg 
      gttgtggtc gcgtcaacg ccgtcagaa caacctgta 
      cagtatgtt tacatgccg ccaccgttc actgtttcg 
      gattcgaac ggacggaac ttgtctgtt ccggtgcaa 
      ctggtttcg aaaacttcc tcacaggcc gatggtggc 
      aaaaaggag ttcaagctc gcgcccact ccggcgcag 
      ttgggcaaa gcaccgcta caaaggcga caatcgatg 
      gctccgaca acttcaaat aacacagcg caagtcaca 
      agtgaaccg tcagtccct ttgccacca cctcccacc 
      agtgagagc tcaacgcaa gactgcctt gtgtcccca 
      atcccgaaa aagagcctt tttaaacgg aacaaagaa 
      gacggaatg gataaggtt ttggatcaa gtcaacttt 
      gagaaaaaa ttctgttcc ttgcctcaa tttaaacca 
      gaagaatgt caatcacca agtgctatt tcgtcctca 
      agccctggt cttttcgca tacaagaaa cgaaacctt 
      agcttgacg aatagatcg tcagtcgat gaggaatcc 
      gaaagcgaa acgccccaa tccgtgccc aaatcttca 
      tcgtctctg aagccagcc gtgttcttc gggcctgac 
      ttcaacgtc gacaacgtt cgagagatg aacgagatg 
      ggggagggc agctcccca cggacgccg cgcacgccc 
      gggacgtcg aaggagtcc gagaagggg caccgaaag 
      attctggag cagcgtcgg cagttagtg atgcagttg 
      ttccacgaa cacacgtat tttccgtct gcgcaagcg 
      acgtcgaac tttcagtcg cagcattca gatatattt 
      ccgagcaaa gcgagtttg cagttgaaa attcgtgaa 
      gtaagacaa aaagtcatg gcgcgaaac aattcaact 
      ccgcacagt gcaaatagt caaagtagt ccagtcact 
      ccagcagaa cccttaaat gtatcctct agtagttag
    mRNA sequence
    • >TC004697 RA
      ggtccccga ttttaatga atttcaact tttgtagtg 
      taactacaa gcaactaat tggcaataa agcgtgtta 
      tttatacca acattcatt catttcgtt attgattgg 
      ggaaaacaa gtgcggacc tgtttccag gcccaaatt 
      cggattttt tgtgttcga ttgttggca atagatacg 
      tgactaggg tcacaccag acgaagtca cgaattttt 
      gccgaaagt gagtgtaaa tctgaaatt gtttttgat 
      aaatggaaa ttggcgcct cttccgaaa tgacaagat 
      cgttcggcg acaatcgag tggccttgg cttgttgtt 
      gtttgttga atttcggtg ggctgtggt ggcacactt 
      gggagtgat ttttgactt ttgacagct gggtccttt 
      tggaaaatt cggggattt gaggtgtaa tccacgctg 
      agttgtgta aaccgagct ttgttttat ctccttggg 
      cagcgctgg tacgcatga acctgccag gggggcgag 
      gggcaggaa ggggagtgt tctgtttct gtgtctgag 
      tttgtgggt gtcgcgaaa agttgtgat cgtatgaaa 
      caaaacgtt aggtaatag gaagagacg aacttttgc 
      cgaatccta ataatagag agtctccaa gccggagtg 
      tgttcgctg tggtggcat ctacacgtg ttgatcggg 
      catcagctc catgacggc taaatatat ttatttact 
      atgcagtag tataatagc atccattca taaaccagt 
      tgttgtctc cggaattgg ccaatgtca aaagttgac 
      gtcttcata aatgatgca cacgggagc tatgttgac 
      aaatagaag gtacaatgc gaacgcctc gggaaaacc 
      gttccgaac ttactcata acgaactgc gagcctagc 
      cacagccac accattcaa tccagcaaa atggatatg 
      ggtgtccga aaactgccg aaaaaacga aaatttgac 
      ccctccgaa ctggaggaa aacacgact gcttcgtac 
      attcctgtg tctgttgtt caaagtgca ccgcaagcc 
      acagccgtc gattactcc tgccctacc aataagcaa 
      aacaccgtc gatctcagc gagtggtgc gaccacagg 
      gtgctcgcc aagcagggc gactggtac taccctggg 
      gtcatcagg gaggcccga ggaagcaat ataaccgtg 
      gagctggac ggcaaggag gagaagctg gtgcacttc 
      accaacgtg tttgataac gagtgttac aacgtgata 
      ggggacgcg agtccgtcc aagaacgag gtgacgctc 
      ggggcgcgc gtttgcgtc cgtcagaac cagcaacaa 
      atgttcgtg gagggtgtg gtatttaac atttgtaac 
      gagggacaa gtcgtgcgt ttcgtggtg gccgtaatc 
      ggcgaaaaa cagttgaaa ataacggtt aaacggtca 
      gaactgcgc cttttgcgc cctccatgg tacgatgag 
      ctcgtggag cctttgatc caggagtcc ataacgccg 
      aaattggag tactttgca aacacgacg gtgtcgccc 
      cacacaccg gtctccgca tgcactccc caatcgaac 
      ggtcgcaac tatgacgac tactgcgag agcgaagaa 
      gacgagttg cgaagaggt gatattacg tttaattcc 
      gaggtagac gctaagctt tcagggagt tcgaagcgg 
      agtagtatg catagtaga ggaagctcc tccagcagt 
      ataactccc cgatctcag ccgaccact ccgcggagc 
      caagccacg acccctcac aagtataag aaaggcgac 
      gtggtgtcg aacccaaac ggcatacgc aaaaagttc 
      aacgggaaa cagtggagg aggttgtgt tcgaaagac 
      ggttgtacg aaagaatct caaaggcgg gggtactgt 
      tcaaggcat cttagcctc aaaggtaat agccttcga 
      agtggtccc gcgtttccg cgttctaat agcaaagca 
      gatggtgag gatacttcc agagattcg gaaacttcg 
      ccgaattgt aacgatcga aggatcacc ggacggttc 
      gaccaagag gaaactgac gcggccaat atgttagtt 
      tctttaggt agttcgcga tcggccact ccggcctat 
      tcgcccaat caccaaggc tcatcgcct catacaatg 
      caatcaccg atcacagtc gggtcccga caaaacgtt 
      ttcatgcct attatcagt ccaggcttg caaaaaatc 
      aactcgccg gggccacaa ggatacaac acggcatat 
      caccaaccc ctaccttta agacccgag tcagttcgg 
      ccgatccag ggcgtgcca agtgtcatt cgactgacc 
      tccagtcct cgtcagtgg ggtcctaac tcgaccgac 
      caccaacaa ggcgtgatt ctgcagcag gctttgacg 
      acaaaccca cctgtagaa accgaaaac acccaaatc 
      caatcagga actgttcta atgcattca actaatgca 
      agtctacta ccagttata gtcaatcca actcagctg 
      ttacctgtt ttgccttct gcatcacag tccgttgta 
      aaaaaggtg atccctagc ggcaccgtg ccccagccc 
      acgccgcca gtgatcgtc aaaacggag caagttgcg 
      tcgaatttg caacagaac cacaaggac ccagtgatc 
      caccacgcg ccgcagccg cgcatttcc gttcagtcg 
      cacaccgcg acctcgccc cagttcacc caaacgcct 
      ccgcggcct gtggccccc cagccgaag atctcgacg 
      aattcccaa tcagccttc gtaatcccc tggcactcg 
      atcgttccg attttgacc gcttgtcct gaatccaac 
      tcgccgccc ccttccgag ctctcgccc ccgttatca 
      gcgccccct gttcccatc tcagcgacg aaaatggac 
      gtacctgat gacgacgcc gaagaactg attccaatt 
      ccggccgaa gaagacgac gatgttttc gaaaatgaa 
      ccaacggat gcgagtttg aacgaaaat tcgacgagc 
      aaacggcgg acgcagtca ctgagctcg ctacagacc 
      ccgaaggat aataatttg gtcaagaat aaggaccgg 
      attcggcgg cctatgaat gctttcatg attttttcg 
      aaacgacat cgggctttg gtccatcag aggcacccc 
      aatcaggac aacaggacc gtatcgaag attttgggc 
      gagtggtgg tacgcccta ggcccggaa cagaagcgg 
      aagtatcac gagttggcg tcggaggtc aaggaggca 
      cacttcaag gcgcatccg gagtggaaa tggtgcaac 
      aaggaccgc cggaaatcg tcaaccggt tcaactcgc 
      agcaagttg agttcgacc ggtgacagt gccgaagtg 
      ggcgaagtg ccgatgagc ccccagccg ttgaactct 
      ccggcgcct ttggcggag ccccctcgc caaatcctc 
      ccagacaac cctggggac gtctccgac gacgaccag 
      atggtcata atcgaggag ccgggaacc gtcgaaatc 
      gacttgaaa tgcaaagaa aaagtaacc gattcggac 
      tctgaatcg cagagcgat ttggaagga ggcgagaac 
      cgcttctcg cagcagcgt ttcagtccc gtgtccaaa 
      accggcacg gacgtcacc tacaggcct aaaccgatc 
      aaagcaata atgccaacc tcggacagc tcgaccaag 
      tacagcccg gttgcgagt tcgagtagt ttgggtttt 
      cactactcg ccggtgaat ccctcggga gttaccggt 
      ttccagccc acagggggc gctttcaag caagcaccg 
      caggccaaa tcagtgatt cagatcggc gatagtcat 
      cagaatagt caatttggg agtcaacaa ccggtcaca 
      gttgcgatt tttacggga cagtcggtt tgtttgtcg 
      aatgatacg gagcgttcg caaccggtg gttgtggtc 
      gcgtcaacg ccgtcagaa caacctgta cagtatgtt 
      tacatgccg ccaccgttc actgtttcg gattcgaac 
      ggacggaac ttgtctgtt ccggtgcaa ctggtttcg 
      aaaacttcc tcacaggcc gatggtggc aaaaaggag 
      ttcaagctc gcgcccact ccggcgcag ttgggcaaa 
      gcaccgcta caaaggcga caatcgatg gctccgaca 
      acttcaaat aacacagcg caagtcaca agtgaaccg 
      tcagtccct ttgccacca cctcccacc agtgagagc 
      tcaacgcaa gactgcctt gtgtcccca atcccgaaa 
      aagagcctt tttaaacgg aacaaagaa gacggaatg 
      gataaggtt ttggatcaa gtcaacttt gagaaaaaa 
      ttctgttcc ttgcctcaa tttaaacca gaagaatgt 
      caatcacca agtgctatt tcgtcctca agccctggt 
      cttttcgca tacaagaaa cgaaacctt agcttgacg 
      aatagatcg tcagtcgat gaggaatcc gaaagcgaa 
      acgccccaa tccgtgccc aaatcttca tcgtctctg 
      aagccagcc gtgttcttc gggcctgac ttcaacgtc 
      gacaacgtt cgagagatg aacgagatg ggggagggc 
      agctcccca cggacgccg cgcacgccc gggacgtcg 
      aaggagtcc gagaagggg caccgaaag attctggag 
      cagcgtcgg cagttagtg atgcagttg ttccacgaa 
      cacacgtat tttccgtct gcgcaagcg acgtcgaac 
      tttcagtcg cagcattca gatatattt ccgagcaaa 
      gcgagtttg cagttgaaa attcgtgaa gtaagacaa 
      aaagtcatg gcgcgaaac aattcaact ccgcacagt 
      gcaaatagt caaagtagt ccagtcact ccagcagaa 
      cccttaaat gtatcctct agtagttag ctagtaatg 
      tgcaataac gtgttgtct tccttctca tatattatc 
      gtgaagtta actgaggag aacggttag tgaatccct 
      ctctcgaga gaaacgaac agttgttgg cgtagtgca 
      attattgtg aatccatgt catttgtat tcattttat 
      ataatttat tgtatcgaa attttatta agtgtacac 
      ttgtaattg taaatccca cgattgcca tgtaaaatg 
      ttatcagat attaaatca ctattttta aaatttatt 
      tggcccaag tcgtcaagt gcaccttca atgttccaa 
      ttgttattg ttgcgatga ctggagtgt ataatgttt 
      atgatcaca gttgcactt tctcggcac taaacgttg 
      ccatatcga tgttacgtt tccaccaga aaggtgctg 
      tttcgttag tttcctggt gctcaatag ttttagcac 
      ttgactgta tcacgactg gcctatatg tgcagacgg 
      aattgcatt gtttcagtt ttaatgaag gctgcaaat 
      atgggttgg tttaattta agaattaag ttcgccttt 
      tgcaatact tgagctttt caaatttac ttgtattat 
      ttagtattg ttaaatata ttttttaat tttccaaat 
      atagagttc tttaaatta ttcattatg gctttaaat 
      acgtaaaac ctgttcatt gataagtgt agcattttg 
      taactaatg atctctata gtgaatctt tgtgtattc 
      agctgtact tcgcaggtt gtcaatttt aagactaac 
      gtaacgtta aaattcgca acttgttta ggaaatagt 
      gaagctaga ccgcttgga ttatttttc taggtataa 
      ttaattaat tttgatctc agttaacgt tttccatgt 
      gaatattat ttcaattcc taagtaggt agtacgtat 
      tcacagacc tataaatac atagatata tattat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY