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Gene information
General information
TC004691 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 145362..149279
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004691
    iBeetle screen iB_06544

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004691 RA
      MNSDKAATK RKPEPNNGG CTGTSTNED AVQAQTPSV 
      AEESPNHLL YKKMEKIVE KMQDETTGV PVRTVKSFM 
      SKIPSVFTG SDLIMWMMK NLDVEDQQE ALHLAHLMA 
      AHGYFFPID DHMLTVKND NTFYRFQTP YFWPSNCWE 
      PENTDYAVY LCKRTMQNK TRLELADYE AENLARLQK 
      MFSRKWEFI FMQAEAQSK VDKKRDKLE RKVLDSQER 
      AFWDVHRPM PGCVNTTEI DIKKACQMN KPLHGLKNM 
      HVGPPRMSP STSGINSNS SQDLLKKQI ALLKSRLER 
      RNIKVSKAA ESFVAYYEQ YLEYDSFFV APEYPNPWI 
      SDNPELWEQ EKQAKDISA RRVKRWAFS LQELLQDPV 
      GREHFVKFL DKEFSGENL KFWEAVQEL KALPQSEVQ 
      SKVQEIWME FLAPDASCP INVDCKSYE ITKKNMESQ 
      DRWSFDCAA AHVYHLMKS DSYSRYLRS EMYKEFLNG 
      SKKKTSVKG IRSIVSFSA RKDNVT
    CDS sequence
    • >TC004691 RA
      atgaactcc gacaaggct gccaccaag cgcaagccg 
      gagcctaac aatgggggc tgtaccgga acgagcact 
      aacgaagac gctgtccaa gcgcaaacc ccgtctgtg 
      gctgaggag tcccccaac cacctcttg tacaaaaag 
      atggaaaaa attgttgag aaaatgcaa gatgagacc 
      acgggggtg ccggtgcgt actgtgaag agctttatg 
      tctaagatc ccttcggta ttcacaggt tctgattta 
      attatgtgg atgatgaaa aacctagac gttgaagat 
      caacaagaa gccttacat cttgcacac cttatggct 
      gcacacgga tattttttt cccatcgac gatcatatg 
      cttactgtc aaaaacgat aatactttt tatagattc 
      caaacacca tatttttgg ccatccaac tgctgggaa 
      cctgagaat actgactat gctgtctac ctttgcaag 
      agaacaatg caaaataaa acacgatta gagcttgcc 
      gactacgaa gccgaaaat ctggctcgt ttgcagaaa 
      atgttctca cggaagtgg gaatttatt ttcatgcaa 
      gcggaagcg cagagcaaa gttgataag aagagagat 
      aagctggag aggaaggtc ttggacagc caggagcgg 
      gctttctgg gatgtgcat cgtccaatg cctgggtgc 
      gtgaacacc actgagatt gatattaag aaagcgtgc 
      caaatgaac aaacctctg catggcttg aagaacatg 
      cacgttggt cctccacgt atgtctccg tcaacatcg 
      ggtatcaac agtaatagt tcgcaagac ttactcaag 
      aaacaaatc gcactctta aaatctaga ttagagaga 
      cgaaacatc aaagtatcg aaagcggca gaatcattt 
      gtagcttat tatgagcag tatttggaa tatgacagt 
      tttttcgtt gcgcccgaa tacccaaac ccttggata 
      tccgataat cccgaactg tgggaacag gagaaacaa 
      gccaaagac atatcagct cgtcgggtg aaacgatgg 
      gcgtttagt ttgcaagag ttgttgcag gatccagtc 
      gggagggaa cattttgtt aagtttctt gataaggag 
      tttagtgga gagaacctc aagttttgg gaagctgtc 
      caggaacta aaagcgctt cctcagagc gaagtccag 
      agtaaagtc caggagatt tggatggag tttttggcg 
      ccggatgca agctgtcca atcaatgtt gactgcaag 
      tcctatgaa atcactaag aagaatatg gaaagccag 
      gatagatgg agctttgac tgtgcagcg gcccacgtt 
      tatcacttg atgaagagt gacagttat tcaaggtac 
      ctaagatca gaaatgtac aaggagttt ttgaacgga 
      tcgaagaaa aaaacttcg gtgaaaggg attcgctcc 
      atcgtgtca ttttcagca cgtaaagac aacgtaact 
      taa
    mRNA sequence
    • >TC004691 RA
      caaagaaaa tatcgatgg taactgtga tggtagttg 
      tatgtagaa aaataaata agccgtttt gtgataatt 
      tttgtgaaa atagggctg tttcgcgtg gggacgcag 
      cgtgaaaat gtgggcgag cttgaaagc gagctaatt 
      cgggcggtg gcaataaat ttttaatta aaaaaagat 
      gcagaaaac agggtttcc tgtgcagca gttttggtt 
      acaaatttt aatggagat ttgcgatga attcggtcg 
      tttgtaatt gttttaaaa ttgaatgag cgttggggt 
      tgtttgtaa tgttaatta attaacgtg agtggtgtt 
      ggtgtagag aagggagga cggggctcc tcctaaacc 
      attgatatg gtcacgatg aactccgac aaggctgcc 
      accaagcgc aagccggag cctaacaat gggggctgt 
      accggaacg agcactaac gaagacgct gtccaagcg 
      caaaccccg tctgtggct gaggagtcc cccaaccac 
      ctcttgtac aaaaagatg gaaaaaatt gttgagaaa 
      atgcaagat gagaccacg ggggtgccg gtgcgtact 
      gtgaagagc tttatgtct aagatccct tcggtattc 
      acaggttct gatttaatt atgtggatg atgaaaaac 
      ctagacgtt gaagatcaa caagaagcc ttacatctt 
      gcacacctt atggctgca cacggatat ttttttccc 
      atcgacgat catatgctt actgtcaaa aacgataat 
      actttttat agattccaa acaccatat ttttggcca 
      tccaactgc tgggaacct gagaatact gactatgct 
      gtctacctt tgcaagaga acaatgcaa aataaaaca 
      cgattagag cttgccgac tacgaagcc gaaaatctg 
      gctcgtttg cagaaaatg ttctcacgg aagtgggaa 
      tttattttc atgcaagcg gaagcgcag agcaaagtt 
      gataagaag agagataag ctggagagg aaggtcttg 
      gacagccag gagcgggct ttctgggat gtgcatcgt 
      ccaatgcct gggtgcgtg aacaccact gagattgat 
      attaagaaa gcgtgccaa atgaacaaa cctctgcat 
      ggcttgaag aacatgcac gttggtcct ccacgtatg 
      tctccgtca acatcgggt atcaacagt aatagttcg 
      caagactta ctcaagaaa caaatcgca ctcttaaaa 
      tctagatta gagagacga aacatcaaa gtatcgaaa 
      gcggcagaa tcatttgta gcttattat gagcagtat 
      ttggaatat gacagtttt ttcgttgcg cccgaatac 
      ccaaaccct tggatatcc gataatccc gaactgtgg 
      gaacaggag aaacaagcc aaagacata tcagctcgt 
      cgggtgaaa cgatgggcg tttagtttg caagagttg 
      ttgcaggat ccagtcggg agggaacat tttgttaag 
      tttcttgat aaggagttt agtggagag aacctcaag 
      ttttgggaa gctgtccag gaactaaaa gcgcttcct 
      cagagcgaa gtccagagt aaagtccag gagatttgg 
      atggagttt ttggcgccg gatgcaagc tgtccaatc 
      aatgttgac tgcaagtcc tatgaaatc actaagaag 
      aatatggaa agccaggat agatggagc tttgactgt 
      gcagcggcc cacgtttat cacttgatg aagagtgac 
      agttattca aggtaccta agatcagaa atgtacaag 
      gagtttttg aacggatcg aagaaaaaa acttcggtg 
      aaagggatt cgctccatc gtgtcattt tcagcacgt 
      aaagacaac gtaacttaa atttaaaaa tcgaaaaca 
      attacgtga gtctcaaaa tttcattct aattatgac 
      ttatccaaa tattggtaa gttcatcta tcgacaata 
      caaatattg ccagtggca gatttttgt tgacttaat 
      tgtttgtaa caaaagttg gacaacttt gcttgttat 
      aaatgtcaa aactattat gtttaccta ttccgtata 
      tattttttg gaacaagtt atgcatggc gtttagtaa 
      attgatatt catataatg atggaactc atgatatta 
      attgtgttg ttgttttaa cagctatag tgacccagt 
      gatattatt ttagtaaca gtccgttct tatgatatt 
      aaaagtgta aataaggtt actagcact gaaacggtt 
      tattgctat gtgatactt gcacaaaca agtttgatt 
      ttcaaaata actatatat ttatataac aaaggaaaa 
      tagcgagtg tacataagt gtgtatata acgttatta 
      tttggactg tgtcactgt atctgttac aaattaatt 
      tgtagttga tttaaagtc tgtgcagtg tagttatac 
      cactttgag gcatttagt aaaaataaa acactgcca 
      tatacaaat taactaagt ttagttaat gtatgaaaa 
      caattgtat atgtgagcg ctacctata tttttttta 
      attaattta tttagctca ggtgtacgc tatagtatt 
      agtaaacga gaataggcc gcaccaatt tttttcctc 
      agacagtgc aattacaat atcaccaaa tctagtgct 
      ttcattcat ttgtatagg ttcaaaatg ccaaatggt 
      tttatattt ggggtttta tagtgtacc cacgacact 
      agattaaat tttctcaat attggtgta caatgctta 
      ggaaatata ctgataatt atgttataa aggaattga 
      gccatatta tctcattga ttatccata tgtagatgg 
      gtgattcca cacagaatt ccaataaat tatatcata 
      cgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY