| General information | |||
| TC004690 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 166701..170239 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004690 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06543 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC004690 RA
MAEIGPIQY FICGGFGGI CTVVVGHPL DTIKVRLQT MPIPKPGEK PLYSGTLDC LKTTVRNEG IRGLYKGMG APLLGVAPI FAISFMGYG VGKKIFGPG DGQHYSYLQ YFTAGAFSG IFTTTIMAP GERIKCLLQ IQQASTGPK TYSGPVDVV KKLYKEGGI RSIYRGSGA TLLRDIPAS GMYFLTYEA IKDYITDHG KESPSILGT IFAGGAAGI ANWAVGMPP DVLKSRLQT APEGTYPNG IRDVFKKLM LTEGPGALY KGITPVLLR AFPANAACF VGFELCKTF LAYLSKASP V
- >TC004690 RB
MYFLTYEAI KDYITDHGK ESPSILGTI FAGGAAGIA NWAVGMPPD VLKSRLQTA PEGTYPNGI RDVFKKLML TEGPGALYK GITPVLLRA FPANAACFV GFELCKTFL AYLSKASPV
- >TC004690 RC
MAEIGPIQY FICGGFGGI CTVVVGHPL DTIKVRLQT MPIPKPGEK PLYSGTLDC LKTTVRNEG IRGLYKGMG APLLGVAPI FAISFMGYG VGKKIFGPG DGQHYSYLQ YFTAGAFSG IFTTTIMAP GERIKCLLQ IQQASTGPK TYSGPVDVV KKLYKEGGI RSIYRGSGA TLLRGKGFI RKLQKAVKL HN
- >TC004690 RA
atggcagaa atcggcccc atccaatac ttcatctgc gggggcttc ggcggcatt tgcaccgtg gtggtgggc caccccctg gacaccatc aaggtgcgc ttgcaaacc atgcccatc cccaagccg ggcgagaag cccctctat tcgggcacc ctcgactgc ctcaaaacc accgttcgc aacgagggc atccgcggc ttgtacaag ggcatgggg gcgccattg ctgggcgta gcgcccatt ttcgccatt tcgttcatg ggctacggc gtcgggaag aagattttc ggaccgggg gacggccaa cactacagc tatttgcaa tatttcacg gctggggcc ttctcaggg attttcacc acgacgatc atggccccc ggcgagcgg atcaagtgc ctgctgcag atacagcaa gcctcgacg gggcccaag acctacagc ggccccgtg gacgtggtc aagaagctg tacaaggag gggggcatc aggagcatc tacaggggc tcgggggcc acgctgctt cgagacatt ccggccagt ggcatgtat ttcctgact tacgaggcg atcaaagac tacattact gaccacggg aaggagtct ccgagcatt ttggggacg atttttgcc ggcggggct gccgggatt gcgaactgg gctgtgggt atgcccccc gacgtgctg aaaagccgc cttcagacc gcgcctgag ggaacgtac cccaatgga attcgcgat gtgtttaaa aaactgatg ctgactgaa gggccgggg gccctgtac aagggcata accccggtc ttgttgcgt gcattccca gccaatgcc gcgtgtttc gttgggttt gagctatgc aagaccttt ttggcgtat ctgtcaaag gcgtcgcct gtttaa
- >TC004690 RB
atgtatttc ctgacttac gaggcgatc aaagactac attactgac cacgggaag gagtctccg agcattttg gggacgatt tttgccggc ggggctgcc gggattgcg aactgggct gtgggtatg ccccccgac gtgctgaaa agccgcctt cagaccgcg cctgaggga acgtacccc aatggaatt cgcgatgtg tttaaaaaa ctgatgctg actgaaggg ccgggggcc ctgtacaag ggcataacc ccggtcttg ttgcgtgca ttcccagcc aatgccgcg tgtttcgtt gggtttgag ctatgcaag acctttttg gcgtatctg tcaaaggcg tcgcctgtt taa
- >TC004690 RC
atggcagaa atcggcccc atccaatac ttcatctgc gggggcttc ggcggcatt tgcaccgtg gtggtgggc caccccctg gacaccatc aaggtgcgc ttgcaaacc atgcccatc cccaagccg ggcgagaag cccctctat tcgggcacc ctcgactgc ctcaaaacc accgttcgc aacgagggc atccgcggc ttgtacaag ggcatgggg gcgccattg ctgggcgta gcgcccatt ttcgccatt tcgttcatg ggctacggc gtcgggaag aagattttc ggaccgggg gacggccaa cactacagc tatttgcaa tatttcacg gctggggcc ttctcaggg attttcacc acgacgatc atggccccc ggcgagcgg atcaagtgc ctgctgcag atacagcaa gcctcgacg gggcccaag acctacagc ggccccgtg gacgtggtc aagaagctg tacaaggag gggggcatc aggagcatc tacaggggc tcgggggcc acgctgctt cgaggtaag ggttttatt cgaaaatta caaaaggcg gtaaagtta cataattaa
- >TC004690 RA
aaattcatt tcaagtggg acactagcg ccactgtgg tgtagtgta gtttcctgt gtcggattt gtggttagg ttatccttt agttaaatt aatttcaat tgcttgtga ttaagtgtc cttcgtaac aaaatggca gaaatcggc cccatccaa tacttcatc tgcgggggc ttcggcggc atttgcacc gtggtggtg ggccacccc ctggacacc atcaaggtg cgcttgcaa accatgccc atccccaag ccgggcgag aagcccctc tattcgggc accctcgac tgcctcaaa accaccgtt cgcaacgag ggcatccgc ggcttgtac aagggcatg ggggcgcca ttgctgggc gtagcgccc attttcgcc atttcgttc atgggctac ggcgtcggg aagaagatt ttcggaccg ggggacggc caacactac agctatttg caatatttc acggctggg gccttctca gggattttc accacgacg atcatggcc cccggcgag cggatcaag tgcctgctg cagatacag caagcctcg acggggccc aagacctac agcggcccc gtggacgtg gtcaagaag ctgtacaag gaggggggc atcaggagc atctacagg ggctcgggg gccacgctg cttcgagac attccggcc agtggcatg tatttcctg acttacgag gcgatcaaa gactacatt actgaccac gggaaggag tctccgagc attttgggg acgattttt gccggcggg gctgccggg attgcgaac tgggctgtg ggtatgccc cccgacgtg ctgaaaagc cgccttcag accgcgcct gagggaacg taccccaat ggaattcgc gatgtgttt aaaaaactg atgctgact gaagggccg ggggccctg tacaagggc ataaccccg gtcttgttg cgtgcattc ccagccaat gccgcgtgt ttcgttggg tttgagcta tgcaagacc tttttggcg tatctgtca aaggcgtcg cctgtttaa ttacactcg aagcgagta ttgactgca ttcttgtct accgttcta gttagttca ttattttgt tgtgggatg ttttgtgtt caaagttgc tattttata aaatgttac ttgtggtga atgcgccac gataagacg tgatatgat taataaata tattttaat agt
- >TC004690 RB
gaaaaatta agtaacgtc caaaaatgc caataactc atttcagac attccggcc agtggcatg tatttcctg acttacgag gcgatcaaa gactacatt actgaccac gggaaggag tctccgagc attttgggg acgattttt gccggcggg gctgccggg attgcgaac tgggctgtg ggtatgccc cccgacgtg ctgaaaagc cgccttcag accgcgcct gagggaacg taccccaat ggaattcgc gatgtgttt aaaaaactg atgctgact gaagggccg ggggccctg tacaagggc ataaccccg gtcttgttg cgtgcattc ccagccaat gccgcgtgt ttcgttggg tttgagcta tgcaagacc tttttggcg tatctgtca aaggcgtcg cctgtttaa ttacactcg aagcgagta ttgactgca ttcttgtct accgttcta gttagttca ttattttgt tgtgggatg ttttgtgtt caaagttgc tattttata aaatgttac ttgtggtga atgcgccac gataagacg tgatatgat taataaata tattttaat agt
- >TC004690 RC
aaattcatt tcaagtggg acactagcg ccactgtgg tgtagtgta gtttcctgt gtcggattt gtggttagg ttatccttt agttaaatt aatttcaat tgcttgtga ttaagtgtc cttcgtaac aaaatggca gaaatcggc cccatccaa tacttcatc tgcgggggc ttcggcggc atttgcacc gtggtggtg ggccacccc ctggacacc atcaaggtg cgcttgcaa accatgccc atccccaag ccgggcgag aagcccctc tattcgggc accctcgac tgcctcaaa accaccgtt cgcaacgag ggcatccgc ggcttgtac aagggcatg ggggcgcca ttgctgggc gtagcgccc attttcgcc atttcgttc atgggctac ggcgtcggg aagaagatt ttcggaccg ggggacggc caacactac agctatttg caatatttc acggctggg gccttctca gggattttc accacgacg atcatggcc cccggcgag cggatcaag tgcctgctg cagatacag caagcctcg acggggccc aagacctac agcggcccc gtggacgtg gtcaagaag ctgtacaag gaggggggc atcaggagc atctacagg ggctcgggg gccacgctg cttcgaggt aagggtttt attcgaaaa ttacaaaag gcggtaaag ttacataat taatgcgat taaagtggt gtctcaaat taaaatgtt gttgtccat
