Status
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Gene information
General information
TC004690 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 166701..170239
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004690
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06543

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004690 RA
      MAEIGPIQY FICGGFGGI CTVVVGHPL DTIKVRLQT 
      MPIPKPGEK PLYSGTLDC LKTTVRNEG IRGLYKGMG 
      APLLGVAPI FAISFMGYG VGKKIFGPG DGQHYSYLQ 
      YFTAGAFSG IFTTTIMAP GERIKCLLQ IQQASTGPK 
      TYSGPVDVV KKLYKEGGI RSIYRGSGA TLLRDIPAS 
      GMYFLTYEA IKDYITDHG KESPSILGT IFAGGAAGI 
      ANWAVGMPP DVLKSRLQT APEGTYPNG IRDVFKKLM 
      LTEGPGALY KGITPVLLR AFPANAACF VGFELCKTF 
      LAYLSKASP V
    • >TC004690 RB
      MYFLTYEAI KDYITDHGK ESPSILGTI FAGGAAGIA 
      NWAVGMPPD VLKSRLQTA PEGTYPNGI RDVFKKLML 
      TEGPGALYK GITPVLLRA FPANAACFV GFELCKTFL 
      AYLSKASPV
    • >TC004690 RC
      MAEIGPIQY FICGGFGGI CTVVVGHPL DTIKVRLQT 
      MPIPKPGEK PLYSGTLDC LKTTVRNEG IRGLYKGMG 
      APLLGVAPI FAISFMGYG VGKKIFGPG DGQHYSYLQ 
      YFTAGAFSG IFTTTIMAP GERIKCLLQ IQQASTGPK 
      TYSGPVDVV KKLYKEGGI RSIYRGSGA TLLRGKGFI 
      RKLQKAVKL HN
    CDS sequence
    • >TC004690 RA
      atggcagaa atcggcccc atccaatac ttcatctgc 
      gggggcttc ggcggcatt tgcaccgtg gtggtgggc 
      caccccctg gacaccatc aaggtgcgc ttgcaaacc 
      atgcccatc cccaagccg ggcgagaag cccctctat 
      tcgggcacc ctcgactgc ctcaaaacc accgttcgc 
      aacgagggc atccgcggc ttgtacaag ggcatgggg 
      gcgccattg ctgggcgta gcgcccatt ttcgccatt 
      tcgttcatg ggctacggc gtcgggaag aagattttc 
      ggaccgggg gacggccaa cactacagc tatttgcaa 
      tatttcacg gctggggcc ttctcaggg attttcacc 
      acgacgatc atggccccc ggcgagcgg atcaagtgc 
      ctgctgcag atacagcaa gcctcgacg gggcccaag 
      acctacagc ggccccgtg gacgtggtc aagaagctg 
      tacaaggag gggggcatc aggagcatc tacaggggc 
      tcgggggcc acgctgctt cgagacatt ccggccagt 
      ggcatgtat ttcctgact tacgaggcg atcaaagac 
      tacattact gaccacggg aaggagtct ccgagcatt 
      ttggggacg atttttgcc ggcggggct gccgggatt 
      gcgaactgg gctgtgggt atgcccccc gacgtgctg 
      aaaagccgc cttcagacc gcgcctgag ggaacgtac 
      cccaatgga attcgcgat gtgtttaaa aaactgatg 
      ctgactgaa gggccgggg gccctgtac aagggcata 
      accccggtc ttgttgcgt gcattccca gccaatgcc 
      gcgtgtttc gttgggttt gagctatgc aagaccttt 
      ttggcgtat ctgtcaaag gcgtcgcct gtttaa
    • >TC004690 RB
      atgtatttc ctgacttac gaggcgatc aaagactac 
      attactgac cacgggaag gagtctccg agcattttg 
      gggacgatt tttgccggc ggggctgcc gggattgcg 
      aactgggct gtgggtatg ccccccgac gtgctgaaa 
      agccgcctt cagaccgcg cctgaggga acgtacccc 
      aatggaatt cgcgatgtg tttaaaaaa ctgatgctg 
      actgaaggg ccgggggcc ctgtacaag ggcataacc 
      ccggtcttg ttgcgtgca ttcccagcc aatgccgcg 
      tgtttcgtt gggtttgag ctatgcaag acctttttg 
      gcgtatctg tcaaaggcg tcgcctgtt taa
    • >TC004690 RC
      atggcagaa atcggcccc atccaatac ttcatctgc 
      gggggcttc ggcggcatt tgcaccgtg gtggtgggc 
      caccccctg gacaccatc aaggtgcgc ttgcaaacc 
      atgcccatc cccaagccg ggcgagaag cccctctat 
      tcgggcacc ctcgactgc ctcaaaacc accgttcgc 
      aacgagggc atccgcggc ttgtacaag ggcatgggg 
      gcgccattg ctgggcgta gcgcccatt ttcgccatt 
      tcgttcatg ggctacggc gtcgggaag aagattttc 
      ggaccgggg gacggccaa cactacagc tatttgcaa 
      tatttcacg gctggggcc ttctcaggg attttcacc 
      acgacgatc atggccccc ggcgagcgg atcaagtgc 
      ctgctgcag atacagcaa gcctcgacg gggcccaag 
      acctacagc ggccccgtg gacgtggtc aagaagctg 
      tacaaggag gggggcatc aggagcatc tacaggggc 
      tcgggggcc acgctgctt cgaggtaag ggttttatt 
      cgaaaatta caaaaggcg gtaaagtta cataattaa
    mRNA sequence
    • >TC004690 RA
      aaattcatt tcaagtggg acactagcg ccactgtgg 
      tgtagtgta gtttcctgt gtcggattt gtggttagg 
      ttatccttt agttaaatt aatttcaat tgcttgtga 
      ttaagtgtc cttcgtaac aaaatggca gaaatcggc 
      cccatccaa tacttcatc tgcgggggc ttcggcggc 
      atttgcacc gtggtggtg ggccacccc ctggacacc 
      atcaaggtg cgcttgcaa accatgccc atccccaag 
      ccgggcgag aagcccctc tattcgggc accctcgac 
      tgcctcaaa accaccgtt cgcaacgag ggcatccgc 
      ggcttgtac aagggcatg ggggcgcca ttgctgggc 
      gtagcgccc attttcgcc atttcgttc atgggctac 
      ggcgtcggg aagaagatt ttcggaccg ggggacggc 
      caacactac agctatttg caatatttc acggctggg 
      gccttctca gggattttc accacgacg atcatggcc 
      cccggcgag cggatcaag tgcctgctg cagatacag 
      caagcctcg acggggccc aagacctac agcggcccc 
      gtggacgtg gtcaagaag ctgtacaag gaggggggc 
      atcaggagc atctacagg ggctcgggg gccacgctg 
      cttcgagac attccggcc agtggcatg tatttcctg 
      acttacgag gcgatcaaa gactacatt actgaccac 
      gggaaggag tctccgagc attttgggg acgattttt 
      gccggcggg gctgccggg attgcgaac tgggctgtg 
      ggtatgccc cccgacgtg ctgaaaagc cgccttcag 
      accgcgcct gagggaacg taccccaat ggaattcgc 
      gatgtgttt aaaaaactg atgctgact gaagggccg 
      ggggccctg tacaagggc ataaccccg gtcttgttg 
      cgtgcattc ccagccaat gccgcgtgt ttcgttggg 
      tttgagcta tgcaagacc tttttggcg tatctgtca 
      aaggcgtcg cctgtttaa ttacactcg aagcgagta 
      ttgactgca ttcttgtct accgttcta gttagttca 
      ttattttgt tgtgggatg ttttgtgtt caaagttgc 
      tattttata aaatgttac ttgtggtga atgcgccac 
      gataagacg tgatatgat taataaata tattttaat 
      agt
    • >TC004690 RB
      gaaaaatta agtaacgtc caaaaatgc caataactc 
      atttcagac attccggcc agtggcatg tatttcctg 
      acttacgag gcgatcaaa gactacatt actgaccac 
      gggaaggag tctccgagc attttgggg acgattttt 
      gccggcggg gctgccggg attgcgaac tgggctgtg 
      ggtatgccc cccgacgtg ctgaaaagc cgccttcag 
      accgcgcct gagggaacg taccccaat ggaattcgc 
      gatgtgttt aaaaaactg atgctgact gaagggccg 
      ggggccctg tacaagggc ataaccccg gtcttgttg 
      cgtgcattc ccagccaat gccgcgtgt ttcgttggg 
      tttgagcta tgcaagacc tttttggcg tatctgtca 
      aaggcgtcg cctgtttaa ttacactcg aagcgagta 
      ttgactgca ttcttgtct accgttcta gttagttca 
      ttattttgt tgtgggatg ttttgtgtt caaagttgc 
      tattttata aaatgttac ttgtggtga atgcgccac 
      gataagacg tgatatgat taataaata tattttaat 
      agt
    • >TC004690 RC
      aaattcatt tcaagtggg acactagcg ccactgtgg 
      tgtagtgta gtttcctgt gtcggattt gtggttagg 
      ttatccttt agttaaatt aatttcaat tgcttgtga 
      ttaagtgtc cttcgtaac aaaatggca gaaatcggc 
      cccatccaa tacttcatc tgcgggggc ttcggcggc 
      atttgcacc gtggtggtg ggccacccc ctggacacc 
      atcaaggtg cgcttgcaa accatgccc atccccaag 
      ccgggcgag aagcccctc tattcgggc accctcgac 
      tgcctcaaa accaccgtt cgcaacgag ggcatccgc 
      ggcttgtac aagggcatg ggggcgcca ttgctgggc 
      gtagcgccc attttcgcc atttcgttc atgggctac 
      ggcgtcggg aagaagatt ttcggaccg ggggacggc 
      caacactac agctatttg caatatttc acggctggg 
      gccttctca gggattttc accacgacg atcatggcc 
      cccggcgag cggatcaag tgcctgctg cagatacag 
      caagcctcg acggggccc aagacctac agcggcccc 
      gtggacgtg gtcaagaag ctgtacaag gaggggggc 
      atcaggagc atctacagg ggctcgggg gccacgctg 
      cttcgaggt aagggtttt attcgaaaa ttacaaaag 
      gcggtaaag ttacataat taatgcgat taaagtggt 
      gtctcaaat taaaatgtt gttgtccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY