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Gene information
General information
TC004690 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 166701..170239
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004690
    iBeetle screen iB_06543

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004690 RA
      MAEIGPIQY FICGGFGGI CTVVVGHPL DTIKVRLQT 
MPIPKPGEK PLYSGTLDC LKTTVRNEG IRGLYKGMG 
APLLGVAPI FAISFMGYG VGKKIFGPG DGQHYSYLQ 
YFTAGAFSG IFTTTIMAP GERIKCLLQ IQQASTGPK 
TYSGPVDVV KKLYKEGGI RSIYRGSGA TLLRDIPAS 
GMYFLTYEA IKDYITDHG KESPSILGT IFAGGAAGI 
ANWAVGMPP DVLKSRLQT APEGTYPNG IRDVFKKLM 
LTEGPGALY KGITPVLLR AFPANAACF VGFELCKTF 
LAYLSKASP V
    • >TC004690 RB
      MYFLTYEAI KDYITDHGK ESPSILGTI FAGGAAGIA 
NWAVGMPPD VLKSRLQTA PEGTYPNGI RDVFKKLML 
TEGPGALYK GITPVLLRA FPANAACFV GFELCKTFL 
AYLSKASPV
    • >TC004690 RC
      MAEIGPIQY FICGGFGGI CTVVVGHPL DTIKVRLQT 
MPIPKPGEK PLYSGTLDC LKTTVRNEG IRGLYKGMG 
APLLGVAPI FAISFMGYG VGKKIFGPG DGQHYSYLQ 
YFTAGAFSG IFTTTIMAP GERIKCLLQ IQQASTGPK 
TYSGPVDVV KKLYKEGGI RSIYRGSGA TLLRGKGFI 
RKLQKAVKL HN
    CDS sequence
    • >TC004690 RA
      atggcagaa atcggcccc atccaatac ttcatctgc 
gggggcttc ggcggcatt tgcaccgtg gtggtgggc 
caccccctg gacaccatc aaggtgcgc ttgcaaacc 
atgcccatc cccaagccg ggcgagaag cccctctat 
tcgggcacc ctcgactgc ctcaaaacc accgttcgc 
aacgagggc atccgcggc ttgtacaag ggcatgggg 
gcgccattg ctgggcgta gcgcccatt ttcgccatt 
tcgttcatg ggctacggc gtcgggaag aagattttc 
ggaccgggg gacggccaa cactacagc tatttgcaa 
tatttcacg gctggggcc ttctcaggg attttcacc 
acgacgatc atggccccc ggcgagcgg atcaagtgc 
ctgctgcag atacagcaa gcctcgacg gggcccaag 
acctacagc ggccccgtg gacgtggtc aagaagctg 
tacaaggag gggggcatc aggagcatc tacaggggc 
tcgggggcc acgctgctt cgagacatt ccggccagt 
ggcatgtat ttcctgact tacgaggcg atcaaagac 
tacattact gaccacggg aaggagtct ccgagcatt 
ttggggacg atttttgcc ggcggggct gccgggatt 
gcgaactgg gctgtgggt atgcccccc gacgtgctg 
aaaagccgc cttcagacc gcgcctgag ggaacgtac 
cccaatgga attcgcgat gtgtttaaa aaactgatg 
ctgactgaa gggccgggg gccctgtac aagggcata 
accccggtc ttgttgcgt gcattccca gccaatgcc 
gcgtgtttc gttgggttt gagctatgc aagaccttt 
ttggcgtat ctgtcaaag gcgtcgcct gtttaa
    • >TC004690 RB
      atgtatttc ctgacttac gaggcgatc aaagactac 
attactgac cacgggaag gagtctccg agcattttg 
gggacgatt tttgccggc ggggctgcc gggattgcg 
aactgggct gtgggtatg ccccccgac gtgctgaaa 
agccgcctt cagaccgcg cctgaggga acgtacccc 
aatggaatt cgcgatgtg tttaaaaaa ctgatgctg 
actgaaggg ccgggggcc ctgtacaag ggcataacc 
ccggtcttg ttgcgtgca ttcccagcc aatgccgcg 
tgtttcgtt gggtttgag ctatgcaag acctttttg 
gcgtatctg tcaaaggcg tcgcctgtt taa
    • >TC004690 RC
      atggcagaa atcggcccc atccaatac ttcatctgc 
gggggcttc ggcggcatt tgcaccgtg gtggtgggc 
caccccctg gacaccatc aaggtgcgc ttgcaaacc 
atgcccatc cccaagccg ggcgagaag cccctctat 
tcgggcacc ctcgactgc ctcaaaacc accgttcgc 
aacgagggc atccgcggc ttgtacaag ggcatgggg 
gcgccattg ctgggcgta gcgcccatt ttcgccatt 
tcgttcatg ggctacggc gtcgggaag aagattttc 
ggaccgggg gacggccaa cactacagc tatttgcaa 
tatttcacg gctggggcc ttctcaggg attttcacc 
acgacgatc atggccccc ggcgagcgg atcaagtgc 
ctgctgcag atacagcaa gcctcgacg gggcccaag 
acctacagc ggccccgtg gacgtggtc aagaagctg 
tacaaggag gggggcatc aggagcatc tacaggggc 
tcgggggcc acgctgctt cgaggtaag ggttttatt 
cgaaaatta caaaaggcg gtaaagtta cataattaa
    mRNA sequence
    • >TC004690 RA
      aaattcatt tcaagtggg acactagcg ccactgtgg 
tgtagtgta gtttcctgt gtcggattt gtggttagg 
ttatccttt agttaaatt aatttcaat tgcttgtga 
ttaagtgtc cttcgtaac aaaatggca gaaatcggc 
cccatccaa tacttcatc tgcgggggc ttcggcggc 
atttgcacc gtggtggtg ggccacccc ctggacacc 
atcaaggtg cgcttgcaa accatgccc atccccaag 
ccgggcgag aagcccctc tattcgggc accctcgac 
tgcctcaaa accaccgtt cgcaacgag ggcatccgc 
ggcttgtac aagggcatg ggggcgcca ttgctgggc 
gtagcgccc attttcgcc atttcgttc atgggctac 
ggcgtcggg aagaagatt ttcggaccg ggggacggc 
caacactac agctatttg caatatttc acggctggg 
gccttctca gggattttc accacgacg atcatggcc 
cccggcgag cggatcaag tgcctgctg cagatacag 
caagcctcg acggggccc aagacctac agcggcccc 
gtggacgtg gtcaagaag ctgtacaag gaggggggc 
atcaggagc atctacagg ggctcgggg gccacgctg 
cttcgagac attccggcc agtggcatg tatttcctg 
acttacgag gcgatcaaa gactacatt actgaccac 
gggaaggag tctccgagc attttgggg acgattttt 
gccggcggg gctgccggg attgcgaac tgggctgtg 
ggtatgccc cccgacgtg ctgaaaagc cgccttcag 
accgcgcct gagggaacg taccccaat ggaattcgc 
gatgtgttt aaaaaactg atgctgact gaagggccg 
ggggccctg tacaagggc ataaccccg gtcttgttg 
cgtgcattc ccagccaat gccgcgtgt ttcgttggg 
tttgagcta tgcaagacc tttttggcg tatctgtca 
aaggcgtcg cctgtttaa ttacactcg aagcgagta 
ttgactgca ttcttgtct accgttcta gttagttca 
ttattttgt tgtgggatg ttttgtgtt caaagttgc 
tattttata aaatgttac ttgtggtga atgcgccac 
gataagacg tgatatgat taataaata tattttaat 
agt
    • >TC004690 RB
      gaaaaatta agtaacgtc caaaaatgc caataactc 
atttcagac attccggcc agtggcatg tatttcctg 
acttacgag gcgatcaaa gactacatt actgaccac 
gggaaggag tctccgagc attttgggg acgattttt 
gccggcggg gctgccggg attgcgaac tgggctgtg 
ggtatgccc cccgacgtg ctgaaaagc cgccttcag 
accgcgcct gagggaacg taccccaat ggaattcgc 
gatgtgttt aaaaaactg atgctgact gaagggccg 
ggggccctg tacaagggc ataaccccg gtcttgttg 
cgtgcattc ccagccaat gccgcgtgt ttcgttggg 
tttgagcta tgcaagacc tttttggcg tatctgtca 
aaggcgtcg cctgtttaa ttacactcg aagcgagta 
ttgactgca ttcttgtct accgttcta gttagttca 
ttattttgt tgtgggatg ttttgtgtt caaagttgc 
tattttata aaatgttac ttgtggtga atgcgccac 
gataagacg tgatatgat taataaata tattttaat 
agt
    • >TC004690 RC
      aaattcatt tcaagtggg acactagcg ccactgtgg 
tgtagtgta gtttcctgt gtcggattt gtggttagg 
ttatccttt agttaaatt aatttcaat tgcttgtga 
ttaagtgtc cttcgtaac aaaatggca gaaatcggc 
cccatccaa tacttcatc tgcgggggc ttcggcggc 
atttgcacc gtggtggtg ggccacccc ctggacacc 
atcaaggtg cgcttgcaa accatgccc atccccaag 
ccgggcgag aagcccctc tattcgggc accctcgac 
tgcctcaaa accaccgtt cgcaacgag ggcatccgc 
ggcttgtac aagggcatg ggggcgcca ttgctgggc 
gtagcgccc attttcgcc atttcgttc atgggctac 
ggcgtcggg aagaagatt ttcggaccg ggggacggc 
caacactac agctatttg caatatttc acggctggg 
gccttctca gggattttc accacgacg atcatggcc 
cccggcgag cggatcaag tgcctgctg cagatacag 
caagcctcg acggggccc aagacctac agcggcccc 
gtggacgtg gtcaagaag ctgtacaag gaggggggc 
atcaggagc atctacagg ggctcgggg gccacgctg 
cttcgaggt aagggtttt attcgaaaa ttacaaaag 
gcggtaaag ttacataat taatgcgat taaagtggt 
gtctcaaat taaaatgtt gttgtccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY