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Gene information
General information
TC004686 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 219972..222469
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004686
    iBeetle screen iB_06542

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004686 RA
      MTDNYPEHL NPFADEKPR PKLTIRDSF KELKRNLRQ 
SFRIKKKDN DEVTLRQKS ETFKRSPLS YPSPQQEAL 
PRSRFRERI STPGSNPFE EEEKRQNAG TTRRRGKKR 
APLPPKSAS SQNVSNLEA SSDGSHWSL TSADTDCSF 
STDNETKPD AMINEFKDF NKNMEALKD DSNNNVVAK 
EKAESNSNI PDLVISCPN EELEESDHL SVISEGGGS 
DLVVKDITS NLIEEKEDE VEVNYCEKK SLYIAGSRD 
DLNDSPTYT GRVSLYITG SCDDLDASN NNEVCNKSV 
TDENSIADA VPTPKQRKN KLSKNILLG TESVCN
    CDS sequence
    • >TC004686 RA
      atgaccgat aattaccct gaacatttg aaccctttt 
gcggacgag aagcccaga cccaaactg acaattcgg 
gactctttc aaagaactt aaaagaaat ttgcggcag 
tcctttcgg atcaaaaag aaggacaac gacgaggtg 
acactccgg caaaagagc gagacgttc aagaggtcg 
ccgttgtcg tacccaagc ccccaacag gaggcactt 
ccccggtca agatttcgg gagcggata agcacccca 
ggctcaaat ccgtttgaa gaagaagag aaaagacaa 
aatgctggc acaacgagg cgaaggggc aaaaagcgg 
gcgcctctg ccccccaaa tcggcgtcc agtcaaaat 
gttagcaat ttggaggct tcctccgat ggttcgcac 
tggagtctg acttctgct gacaccgac tgctccttc 
tcgactgat aacgaaacc aaaccagac gcaatgata 
aacgagttc aaagatttt aacaaaaat atggaagcg 
ctaaaagac gatagtaat aacaatgtg gttgccaaa 
gagaaagcg gagtcaaac agtaacatt ccagaccta 
gtgatatct tgtcccaat gaagaactg gaagaaagt 
gaccatttg tcggtcatt tcggaggga ggcggttca 
gacttagtc gttaaggat attacgagt aatttgatt 
gaggaaaaa gaagatgaa gtagaagtg aattattgc 
gagaagaag tcactttac atcgctgga tctcgtgac 
gatttgaac gactcccca acttacact ggaagagtt 
tccttgtac ataacagga tcatgtgac gaccttgat 
gctagtaat aacaacgaa gtttgtaat aaatctgta 
accgacgaa aatagtatc gctgatgca gttccgaca 
cccaagcaa agaaaaaat aaattgtct aaaaatatc 
cttttggga acggaatca gtctgtaat taa
    mRNA sequence
    • >TC004686 RA
      aattgccac ttttggcac aatttcgcg ccacttgtc 
gcagttcga gcgattatt ttgcgtgga aataatgga 
aacacttaa gtgacgtca ctaagtaat ttaccgtag 
atacatgcg atagtgggg gctagcaca cattcagag 
cagtttctt tagtgtgca gccgtgttg tgcatcaaa 
aaatgaccg ataattacc ctgaacatt tgaaccctt 
ttgcggacg agaagccca gacccaaac tgacaattc 
gggactctt tcaaagaac ttaaaagaa atttgcggc 
agtcctttc ggatcaaaa agaaggaca acgacgagg 
tgacactcc ggcaaaaga gcgagacgt tcaagaggt 
cgccgttgt cgtacccaa gcccccaac aggaggcac 
ttccccggt caagatttc gggagcgga taagcaccc 
caggctcaa atccgtttg aagaagaag agaaaagac 
aaaatgctg gcacaacga ggcgaaggg gcaaaaagc 
gggcgcctc tgcccccca aatcggcgt ccagtcaaa 
atgttagca atttggagg cttcctccg atggttcgc 
actggagtc tgacttctg ctgacaccg actgctcct 
tctcgactg ataacgaaa ccaaaccag acgcaatga 
taaacgagt tcaaagatt ttaacaaaa atatggaag 
cgctaaaag acgatagta ataacaatg tggttgcca 
aagagaaag cggagtcaa acagtaaca ttccagacc 
tagtgatat cttgtccca atgaagaac tggaagaaa 
gtgaccatt tgtcggtca tttcggagg gaggcggtt 
cagacttag tcgttaagg atattacga gtaatttga 
ttgaggaaa aagaagatg aagtagaag tgaattatt 
gcgagaaga agtcacttt acatcgctg gatctcgtg 
acgatttga acgactccc caacttaca ctggaagag 
tttccttgt acataacag gatcatgtg acgaccttg 
atgctagta ataacaacg aagtttgta ataaatctg 
taaccgacg aaaatagta tcgctgatg cagttccga 
cacccaagc aaagaaaaa ataaattgt ctaaaaata 
tccttttgg gaacggaat cagtctgta attaaataa 
agcactgca ttgaatttt ttattgagt ttaaatcaa 
aacttaaca gaaacaata taaggaatg caaattagc 
gaaaactga tttcctata cgttctcat tagtaatag 
taataataa cactgtaac tatgaacaa tccataaat 
caacaaaat aagtctaat ggacggtgc tgcagagct 
ggatgactt tcagagaga cgttccggc agctgagcg 
aggagtcgc tcaggacgc caaacctgt agtgaaatt 
ttgaaaaaa taaactgtt atttattcc ctttccctt 
gtgtttcat tggcgcatc tttggtcaa attgctggc 
cggtgcaga ggcggatta agggccagt cacggtcac 
agtccgcga t
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