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Gene information
General information
TC034896 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15395152..15466933
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034896
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06539

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034896 RA
      MLGVLKRRW KPKRSANVS RIPGADTNT LELDKDKPS 
      RPSVRQVHP VCEDKAHFL AYDKSIMEL ASNDGSPPP 
      YPKRASLDS NPSPTQCYP LAPNICVEA GRIQFIKQE 
      VEDAVDGLP TPPATPRGR VTVTRGLQT DDEELHDSK 
      KSLEERIHE LEMALEAVQ KNELRDKQT IAKLQKQLS 
      RRESAQRDA DRERRLRAD SESRAKQAL QEAARCKSR 
      LKLLTSEFT RMEETVRSM LVYKKQAEQ LRQEKNALT 
      LAFENRCQQ YQNTVTRLN NEIATLRSQ LAQVGSESS 
      SEEILQAHA AALHRHAEE SRKQYERCL DDVANQVVK 
      ALLAQKGLR EEVVHLNSR IHELEAQNR ALTSMLVHQ 
      LRGDTPSPD PEENLPTVI KHFNSFNSD DTPDNHETA 
      LSKFERRLS TNSEILDTK LSFEDKKRH QVLTKLWTE 
      LKGSEVTPK KLLEALGSV DSSLWVQPQ RPVSLNLHL 
      PIVQATKYR RSRPILQSS SKSEEETGN ESPESGNRD 
      EGYSTMSSD VQADLTRSE AVPNRGLED LKEATDETD 
      ITTETRLLV TDNKDPDIL YIPFNLLNL KPRNSYPPG 
      KDMLPFQHI MRSFSDSHL CIKITTAPS PCYSFSSPI 
      SSSPSIFLL DITDKNPLR RAKATSTLW GNTNLEICD 
      ESWPSPAEV ECPTWDAEY IQQWLRLDE TRTTLQQQR 
      DLLELEYDR AELEDWSLS LSNDDLREQ CKRLEALTP 
      GQISISTLP SIQENNALE LEEDSNECL WNNSSYLMD 
      KEGRELVTL LIGENERAW PYAPQQVSP DWSSSSEAE 
      TFSKRSSAA LSDDTGELP GTDFTRDFY RLVKFESTK 
      SLASTSSRS LAENGDTFH NVLNLIAEK QNDATKDFN 
      IEGPGDENY VTLKQLCEE YAKKDEETK HNLAYADEV 
      CLVTPIDVE ISENCSNLS ELPVDICSS VNVELSSTT 
      MSNSDVSTS TPETIISKI PRRKRDTKL APSASSPKK 
      ADDLKPKSK IPKVKQKIV EVVQTAQHH VITSERLPQ 
      CTSGVISNI IEGPPHRAV SFHERATSK DVIDELNRM 
      IKNGEEPNG DKEEGLGAA KKLDEGCRT TGWVHVEKD 
      IDLTDPKAR ANLLDVMMA SVSSDSSPT SSCGGSVAS 
      DSTEDPPDY GHLHRIHRF HRQKKANAA RPELAVVRA 
      SPASRPTII GRPDFFVRY GEKEMEAVA SFDFLDELD 
      EEERIPSLK VSHV
    CDS sequence
    • >TC034896 RA
      atgctgggc gtcttaaag cgacgttgg aaacccaaa 
      aggtctgcc aacgtctct cgaattccc ggcgccgat 
      acaaacaca cttgaattg gacaaggac aagccaagt 
      cgtccttca gtccgtcaa gttcatccg gtttgcgag 
      gacaaagcc cattttttg gcttacgac aagtccatc 
      atggagttg gcctccaat gatgggagt cctccacca 
      tatccgaaa cgagctagt ctggattcg aaccccagc 
      ccaacgcag tgctaccct ctagcgccc aatatttgt 
      gtggaagcg gggagaatt cagtttatc aaacaggaa 
      gtagaggac gctgtggac ggcctgccg acgccgcct 
      gcgacgccc agggggcgg gtgacggtc acgcggggg 
      ctgcagacc gacgacgag gaattgcac gattcgaag 
      aaatcgctc gaggagcga atccacgaa ctcgaaatg 
      gcattggaa gcagtgcag aagaacgaa ttacgcgac 
      aaacaaaca atcgccaag ctacaaaag cagttatcg 
      aggagggaa tcagctcag agggatgct gaccgcgag 
      cgccgcctc cgcgccgac agcgaatca agggcgaag 
      caggccctc caggaggcc gctcgatgc aaatcacga 
      cttaaactg ctcacgagc gagttcact agaatggag 
      gagacggtg cgttcgatg ctggtgtac aagaagcag 
      gccgagcag ttgcgacaa gagaaaaat gcgctaact 
      ttggcgttc gagaatcgt tgtcagcag taccaaaat 
      accgtaacg cgtttaaat aacgaaata gcaactttg 
      cgttcgcaa ttggctcaa gttggaagt gagagcagt 
      tcggaagag attcttcaa gcacatgct gccgccttg 
      cataggcat gccgaagag tcaaggaag cagtacgag 
      aggtgtttg gatgatgtt gcgaatcag gtggtcaaa 
      gctctacta gcacaaaag ggcttacgc gaggaagtc 
      gtccacctg aactcgcgc atccacgag ctcgaggcc 
      cagaaccgc gccttgacc tccatgctg gtgcaccag 
      ctccgtggc gacaccccg tcccccgac ccggaggag 
      aacctcccc accgtgatc aaacacttt aactccttc 
      aactcggac gacactccc gacaaccac gaaacggcg 
      ctctcaaaa ttcgagcgt cgtctctcc acaaattcg 
      gaaatactc gacacgaag ctctccttc gaagacaag 
      aagcggcac caggtgttg acgaaactc tggactgag 
      cttaagggc agcgaagtg acgccgaaa aagctactc 
      gaggctttg gggagtgtg gattcgagt ctgtgggtg 
      cagcctcag aggccggtg tcgttgaat ctccacttg 
      cccattgtg caagcgacc aagtaccgc aggagtcgg 
      cccattctg cagagcagt tccaagagt gaggaagag 
      acggggaat gagtcgcca gagagtggg aatagggat 
      gagggttat tcgaccatg tcttcggat gttcaggcc 
      gacttgaca cggagtgaa gcggtgccg aataggggc 
      ctggaggac ttgaaggaa gccacggat gagactgat 
      attactacc gaaacgcgc cttcttgtg actgacaat 
      aaggatcct gatatactc tacattccg ttcaatttg 
      ttgaatctc aagccgaga aacagttat cctcccggc 
      aaagacatg ctgcccttt caacacatc atgcgaagc 
      ttttccgat tcgcacctc tgtatcaaa ataacaaca 
      gcgccaagt ccttgctac agtttcagc tcgcctatt 
      tcgagcagt ccttcgatt tttctcctc gacatcacc 
      gacaaaaat ccattgcga cgagccaaa gccactagt 
      actttatgg ggtaatacc aacttagaa atctgtgat 
      gagtcctgg ccgagtcca gccgaagtc gagtgtccc 
      acatgggac gctgaatac atccagcag tggttacgt 
      ctcgacgag accagaacc accctccaa cagcaacgc 
      gatttactc gaactcgaa tacgaccga gctgaactg 
      gaggattgg agtttgagt ttgtcaaac gacgatcta 
      agggagcaa tgcaagagg ctagaagcc ctaactccc 
      ggtcaaata tcaatctca acccttcca agcattcaa 
      gagaacaac gcccttgaa ctggaggag gattcaaac 
      gaatgtctt tggaacaac tcgagttac ctgatggac 
      aaagagggc cgtgagctg gtcacgctc ctcattggc 
      gaaaacgag cgggcgtgg ccatacgcc ccccagcaa 
      gtctcgccc gattggtcg agcagctcg gaggcggag 
      accttctcg aagcgatca tcggcggcg ctaagcgac 
      gacaccggg gaactaccc ggcaccgac ttcacacgt 
      gacttctac cgattggtc aaattcgaa agcacgaaa 
      agtctcgcc tcgacctcg tcccgaagc ttggccgaa 
      aacggtgac acatttcac aacgtttta aacctaatc 
      gccgaaaaa caaaacgac gccacgaaa gatttcaat 
      atcgaaggc cccggtgac gagaattac gtcacactg 
      aagcaattg tgcgaagaa tacgcgaag aaagacgaa 
      gaaacgaag cacaatctc gcctatgcg gacgaggtg 
      tgtttggtc acccctatc gatgtggaa atcagcgag 
      aattgctcg aatttgagt gaattaccg gtcgatatt 
      tgctccagt gtcaatgtg gagttgagc tcgaccaca 
      atgagcaat agtgacgtt tcgacaagc acacccgag 
      acgattatt tcgaaaatt ccgcgccgt aaacgcgac 
      acgaagctg gcgccatct gcgtcgtcg ccgaagaag 
      gccgacgat ttgaaacca aagagtaaa attccgaaa 
      gtgaaacaa aaaattgta gaggttgtt cagactgca 
      caacatcac gtgattacg tcggagcga cttccgcag 
      tgtacttcc ggtgtgata agtaatatt attgaaggg 
      ccgccgcat cgggctgtg agttttcat gagagggcg 
      acttcgaag gatgtaatc gacgagttg aatcgaatg 
      attaagaat ggggaggag ccaaacggt gataaggag 
      gaggggcta ggggcggcg aaaaagttg gatgagggg 
      tgcaggaca acggggtgg gtgcatgtc gagaaggat 
      attgacttg acggatcca aaggctaga gcgaacctc 
      cttgacgtg atgatggcc tccgtgtcg agcgactct 
      tcccccacg tcgtcatgc gggggcagt gtagcgagt 
      gactcaacc gaggaccct ccagactat ggtcacttg 
      caccgaatc catcgcttc caccgccag aaaaaagcg 
      aacgccgcc cgccccgag ctggccgtc gtgcgagcg 
      tcgccggcg tcgcgcccg accatcatc gggcggccc 
      gactttttc gtccgctac ggggagaag gagatggaa 
      gcggtcgcg agcttcgat ttcctggat gagctggac 
      gaagaggag cggattcct tcactcaaa gtcagccac 
      gtttaa
    mRNA sequence
    • >TC034896 RA
      catatgacc ccaacttgt ttattttga gcatcagca 
      tcttgattt caggagagg cactcgacg agtgcagtg 
      gtcgtgtaa agtgtctcg taataacgc gagttgctg 
      aaaaaaaaa ttcagcgcg tttcagttt tacgaacgt 
      tttcattcg ctatgtaag aaaaaaaca aaacgcgcg 
      aatggcaac gagtgctgt tttgtgacc tgaaaaatt 
      ctcctcttc catttgctc gtcttgtat gtagtttgg 
      aaaatgctg ggcgtctta aagcgacgt tggaaaccc 
      aaaaggtct gccaacgtc tctcgaatt cccggcgcc 
      gatacaaac acacttgaa ttggacaag gacaagcca 
      agtcgtcct tcagtccgt caagttcat ccggtttgc 
      gaggacaaa gcccatttt ttggcttac gacaagtcc 
      atcatggag ttggcctcc aatgatggg agtcctcca 
      ccatatccg aaacgagct agtctggat tcgaacccc 
      agcccaacg cagtgctac cctctagcg cccaatatt 
      tgtgtggaa gcggggaga attcagttt atcaaacag 
      gaagtagag gacgctgtg gacggcctg ccgacgccg 
      cctgcgacg cccaggggg cgggtgacg gtcacgcgg 
      gggctgcag accgacgac gaggaattg cacgattcg 
      aagaaatcg ctcgaggag cgaatccac gaactcgaa 
      atggcattg gaagcagtg cagaagaac gaattacgc 
      gacaaacaa acaatcgcc aagctacaa aagcagtta 
      tcgaggagg gaatcagct cagagggat gctgaccgc 
      gagcgccgc ctccgcgcc gacagcgaa tcaagggcg 
      aagcaggcc ctccaggag gccgctcga tgcaaatca 
      cgacttaaa ctgctcacg agcgagttc actagaatg 
      gaggagacg gtgcgttcg atgctggtg tacaagaag 
      caggccgag cagttgcga caagagaaa aatgcgcta 
      actttggcg ttcgagaat cgttgtcag cagtaccaa 
      aataccgta acgcgttta aataacgaa atagcaact 
      ttgcgttcg caattggct caagttgga agtgagagc 
      agttcggaa gagattctt caagcacat gctgccgcc 
      ttgcatagg catgccgaa gagtcaagg aagcagtac 
      gagaggtgt ttggatgat gttgcgaat caggtggtc 
      aaagctcta ctagcacaa aagggctta cgcgaggaa 
      gtcgtccac ctgaactcg cgcatccac gagctcgag 
      gcccagaac cgcgccttg acctccatg ctggtgcac 
      cagctccgt ggcgacacc ccgtccccc gacccggag 
      gagaacctc cccaccgtg atcaaacac tttaactcc 
      ttcaactcg gacgacact cccgacaac cacgaaacg 
      gcgctctca aaattcgag cgtcgtctc tccacaaat 
      tcggaaata ctcgacacg aagctctcc ttcgaagac 
      aagaagcgg caccaggtg ttgacgaaa ctctggact 
      gagcttaag ggcagcgaa gtgacgccg aaaaagcta 
      ctcgaggct ttggggagt gtggattcg agtctgtgg 
      gtgcagcct cagaggccg gtgtcgttg aatctccac 
      ttgcccatt gtgcaagcg accaagtac cgcaggagt 
      cggcccatt ctgcagagc agttccaag agtgaggaa 
      gagacgggg aatgagtcg ccagagagt gggaatagg 
      gatgagggt tattcgacc atgtcttcg gatgttcag 
      gccgacttg acacggagt gaagcggtg ccgaatagg 
      ggcctggag gacttgaag gaagccacg gatgagact 
      gatattact accgaaacg cgccttctt gtgactgac 
      aataaggat cctgatata ctctacatt ccgttcaat 
      ttgttgaat ctcaagccg agaaacagt tatcctccc 
      ggcaaagac atgctgccc tttcaacac atcatgcga 
      agcttttcc gattcgcac ctctgtatc aaaataaca 
      acagcgcca agtccttgc tacagtttc agctcgcct 
      atttcgagc agtccttcg atttttctc ctcgacatc 
      accgacaaa aatccattg cgacgagcc aaagccact 
      agtacttta tggggtaat accaactta gaaatctgt 
      gatgagtcc tggccgagt ccagccgaa gtcgagtgt 
      cccacatgg gacgctgaa tacatccag cagtggtta 
      cgtctcgac gagaccaga accaccctc caacagcaa 
      cgcgattta ctcgaactc gaatacgac cgagctgaa 
      ctggaggat tggagtttg agtttgtca aacgacgat 
      ctaagggag caatgcaag aggctagaa gccctaact 
      cccggtcaa atatcaatc tcaaccctt ccaagcatt 
      caagagaac aacgccctt gaactggag gaggattca 
      aacgaatgt ctttggaac aactcgagt tacctgatg 
      gacaaagag ggccgtgag ctggtcacg ctcctcatt 
      ggcgaaaac gagcgggcg tggccatac gccccccag 
      caagtctcg cccgattgg tcgagcagc tcggaggcg 
      gagaccttc tcgaagcga tcatcggcg gcgctaagc 
      gacgacacc ggggaacta cccggcacc gacttcaca 
      cgtgacttc taccgattg gtcaaattc gaaagcacg 
      aaaagtctc gcctcgacc tcgtcccga agcttggcc 
      gaaaacggt gacacattt cacaacgtt ttaaaccta 
      atcgccgaa aaacaaaac gacgccacg aaagatttc 
      aatatcgaa ggccccggt gacgagaat tacgtcaca 
      ctgaagcaa ttgtgcgaa gaatacgcg aagaaagac 
      gaagaaacg aagcacaat ctcgcctat gcggacgag 
      gtgtgtttg gtcacccct atcgatgtg gaaatcagc 
      gagaattgc tcgaatttg agtgaatta ccggtcgat 
      atttgctcc agtgtcaat gtggagttg agctcgacc 
      acaatgagc aatagtgac gtttcgaca agcacaccc 
      gagacgatt atttcgaaa attccgcgc cgtaaacgc 
      gacacgaag ctggcgcca tctgcgtcg tcgccgaag 
      aaggccgac gatttgaaa ccaaagagt aaaattccg 
      aaagtgaaa caaaaaatt gtagaggtt gttcagact 
      gcacaacat cacgtgatt acgtcggag cgacttccg 
      cagtgtact tccggtgtg ataagtaat attattgaa 
      gggccgccg catcgggct gtgagtttt catgagagg 
      gcgacttcg aaggatgta atcgacgag ttgaatcga 
      atgattaag aatggggag gagccaaac ggtgataag 
      gaggagggg ctaggggcg gcgaaaaag ttggatgag 
      gggtgcagg acaacgggg tgggtgcat gtcgagaag 
      gatattgac ttgacggat ccaaaggct agagcgaac 
      ctccttgac gtgatgatg gcctccgtg tcgagcgac 
      tcttccccc acgtcgtca tgcgggggc agtgtagcg 
      agtgactca accgaggac cctccagac tatggtcac 
      ttgcaccga atccatcgc ttccaccgc cagaaaaaa 
      gcgaacgcc gcccgcccc gagctggcc gtcgtgcga 
      gcgtcgccg gcgtcgcgc ccgaccatc atcgggcgg 
      cccgacttt ttcgtccgc tacggggag aaggagatg 
      gaagcggtc gcgagcttc gatttcctg gatgagctg 
      gacgaagag gagcggatt ccttcactc aaagtcagc 
      cacgtttaa tttattttt tgtaacgtc gtatttatt 
      gtctatatg taaattatt ttaatagac ttgtacttt 
      cactctgat tttactatc gacgagtag cgagcttag 
      ataagactg tacataggt tataatcgt atttttttt 
      tattgagac actttgggt tcattcaaa cgaaacgaa 
      ttatgtgta gtacactca aatgtactt ttgtgatat 
      aatgaaatt aataatgga cgttatatt taaccgatt 
      ttatgatga atgcgaggg cctagaaat ttgtgatca 
      tttcattcg gatttttca atagctgtg caataaatt 
      ttcacactt gtggcgaaa tgatctctt ggctaaatc 
      aaagcaatc ttcatggta gatacagat gttgcggtt 
      atgtataaa ctgtgtgta gagtgtatt tttatatag 
      ggtacttat ttatattta gtacaaatt tagacataa 
      atacattat tatgattca a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY