Status
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Gene information
General information
TC034482
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012863,TC012862
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15478539..15539680
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034482
    iBeetle screen iB_06538

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034482 RA
      MLFLLPLLL AAAAGGRPR CGGLLTAAR GILQTPSFP 
      SEFPVPIHC EWVIDAQNF ASPNTSIVV YLTQLYVHE 
      GLTFTEYQV YDRTYQLDG REIHRVNET NVVQVRWVQ 
      SFQNFLVVT LKLDSVDSA HLRVLDGFL DVYGFNITY 
      EIARGPARV DSCTIMDCG FTGICYDHY TKFSCECFP 
      GYSGPHCSS GPNSFCANA NPTCKNGGT CIHVGVSAV 
      KCHCQMGFT GETCQIPVT TLKPGCDNC VQSCPFDGR 
      LENACKCPM MNGTNDRVG FTSTMHLAN LPNLSGNAL 
      KTFIENQVF RTLKTNYSK LDDVSVQNV AFFRSGADV 
      TFHLFALKK DEKRIRAVF SRWADRGYI ANLTVTDKE 
      FVFKPMLDL QSVSINQIG PIRQNDEFI LSCVARGSP 
      TTSFRWFKD GVFVNVTEI TSHKWNRLI KYPHSDDQY 
      TALLGISRS DILDSGTFT CQVEDYGIQ QCLSRRVAI 
      KAPPVIKVE PMSLTVRKG QNLTIKCIS MGDSNNKYT 
      YSWTKNKEL IPVRSETEK YEILYPGGS ILQIFKIEK 
      SVKFSCLVQ DGPISSETS VDIHVVDPS SVPTCPPEY 
      SLNVTWPET APDTDSLQE CPKGYAFPG FVRRSCTLN 
      DGLRPIWRQ PDYSQCTPE QLYKIGLDL RLLQLGYET 
      ATPLQIIQN CSRYLGERQ GLLPGEGTG VLAILRDVI 
      GYCKRARVV TGDLVKEVF EIVDGVLRH QNSLVSQEQ 
      VKLLQEVVS HQIEISAPL LSPSQPLFH LALPTLDVN 
      LMSGDQKEL PFLSANLSG HKWTPHKTR ISSKDPKHG 
      QCVIGVVIY RNLSAFLPT RSVIRVKDG AEVEYEVIS 
      QVVRLWILS RDKNYDYNP HIILEWKQG NHNATKDGW 
      FLKCAMAET AMYAYAWNT TVCTTKILG FNLTRCICP 
      KVGTFALLL TANNIQIEK EEPRPLRYI VLAGCSLCF 
      LSALISTII LTIHWILKR SCVVFLKLQ CSVSITGVM 
      LTFVLVTIS DPSERLFAS VMAILEILF LMGLSAHIS 
      ILLMVYTEM VQLPKSAAS KQTVVGIST GVPVIAVFG 
      NHLAHQTMD VRLKSWWLL AGSLAFNIF LTVGVVIVG 
      LFVLLHVTV LYKLKKLTT VHEKEGKIL QKRITLLKK 
      SAYVFCSLV SATVSSIFY VNFVNIVWW HYEFSVVTM 
      LLGLVVVVC YVVKSDGDF FYLLQGRFK AKQKDGFFG 
      VEATGSPLN FFTKEDAEV ESESIPHHR KSEPSKPIE 
      CFPMSDSCK SGLPECVSG AMESYAHSD AIYCAQTSL 
      ERYNRSPQL HRKTAPFKI EDEVRCRSP DIIATKICT 
      QLDLVASHA PSIVITPEE RPMREIERE IDTVCEKIE 
      LESGSGAGG DNLDGMLDR ISHDLDYLL NRSEEGPRR 
      HSKTNIITE QIIEEVEEE GKVPDAITD ILRTNC
    CDS sequence
    • >TC034482 RA
      atgctcttc ctcctgccg ctgctcctc gccgccgcc 
      gccggtggc cgcccccgg tgcgggggc ctgctcacc 
      gccgcccgg ggcatcctc caaaccccc tcattcccg 
      tcggaattc cccgtcccc atccactgc gaatgggtg 
      atcgatgcc caaaatttc gcctccccc aacacctcc 
      atcgtggtt tacctcacc cagctgtac gtccacgag 
      ggtttgacc ttcaccgag taccaggtg tacgaccgc 
      acttaccag ctcgacggt cgggagatc caccgcgtc 
      aacgagacc aacgtggtg caggtgcga tgggtgcag 
      tcgttccag aattttttg gtcgtgacc ttgaagctg 
      gatagtgtg gatagcgcc catctgagg gtgcttgat 
      gggtttctg gacgtgtat gggtttaat atcacgtat 
      gaaatagcg cgagggccg gccagagtg gactcgtgc 
      actattatg gattgcggt tttacgggg atttgctac 
      gaccattac acaaaattc agctgcgag tgttttccc 
      ggctacagt ggccctcat tgttcttcc ggtcccaac 
      tctttctgt gcgaacgca aacccaacg tgcaaaaac 
      ggaggcact tgcatccat gtgggagtt tccgctgtg 
      aaatgccac tgccagatg ggcttcact ggcgagaca 
      tgccaaatt cccgtcacc acactgaag cccggatgt 
      gacaactgc gtccagagc tgtccgttc gatgggagg 
      ctggagaat gcctgcaag tgtccgatg atgaacgga 
      actaatgat cgggtcgga ttcaccagc accatgcat 
      ttggccaat ctgcccaat ctgagcgga aacgccctc 
      aaaacgttc atcgaaaac caggtattc aggacgctc 
      aagaccaac tactcgaaa ctggacgac gtttcggtg 
      caaaatgtc gcgtttttc aggtcaggt gcagatgta 
      actttccat ttgttcgcg ctgaaaaag gacgagaaa 
      cgcatcagg gcagttttc agcagatgg gctgatagg 
      ggttacatc gccaacttg acagtcaca gacaaggag 
      tttgtcttc aagccgatg ctcgattta cagagcgtg 
      tcaatcaat caaatcggg ccgatccgt caaaacgac 
      gagtttatc ctttcgtgt gtggcgagg ggttcgccc 
      acgacgtcc ttcagatgg ttcaaggac ggcgtgttt 
      gtcaatgtg acggaaatt acaagtcac aaatggaac 
      cgtctcata aaatatccc cattcggat gatcaatac 
      acggccctg ttaggaatc tcaaggagc gatatttta 
      gacagcggc accttcact tgccaagtt gaggattac 
      ggaattcaa caatgcctt tcgagaaga gtcgccatc 
      aaagcaccg ccggttatt aaagtcgaa cctatgagt 
      ctcaccgta cggaaaggc caaaattta actattaag 
      tgcattagc atgggcgac agcaacaac aaatacact 
      tatagctgg accaaaaac aaagaactg attcctgtc 
      aggagcgag acggagaag tacgagatt ttgtatcct 
      ggaggaagc atcttgcaa atattcaaa atcgaaaaa 
      tcggtgaaa ttctcctgc ctggtgcaa gacggtcca 
      atttcgagc gaaaccagc gtcgatatc cacgtggtg 
      gacccgtcc tcggtgccc acgtgcccc ccggagtat 
      tccttgaac gttacatgg ccggaaacg gcccccgac 
      accgatagt ctccaggag tgtcccaaa ggctacgca 
      tttccggga ttcgtccgg cgttcgtgc actctaaac 
      gatggctta cgtcccatc tggcgtcag ccggattac 
      tcccaatgc actcccgag caattatac aaaataggg 
      ttggacctg agattattg caactgggc tacgaaaca 
      gcaacgccg ctccagatc atccaaaac tgctccaga 
      tacctaggg gagaggcaa gggctgttg ccgggggag 
      ggcaccggg gttttggca attttgagg gacgtgatt 
      ggttattgt aaaagggca agggttgtg acgggcgat 
      ttggtcaag gaggtgttc gagattgtg gatggggtg 
      ttgaggcac cagaattcg ctcgtcagc caagagcaa 
      gtgaagctc ctgcaagaa gtcgtcagt caccaaatc 
      gagatctct gcacctctc ctctcgccc tcgcagccc 
      cttttccac ttagccctt ccaaccctt gacgttaat 
      ttaatgtcc ggagatcaa aaagaatta ccatttctc 
      tccgccaac ctcagtggc cacaaatgg accccacac 
      aagactcga atttcatcc aaagacccg aaacacggc 
      caatgcgtg attggagtg gtcatttac cgcaatttg 
      agcgctttt ttaccaaca cggtccgta atccgagta 
      aaagacgga gccgaagtg gaatacgag gtgatatct 
      caagtcgtt cgcttatgg atcctgtca cgggataaa 
      aattacgat tataatcct cacattatc ctcgagtgg 
      aaacaggga aatcacaac gcgaccaaa gacggatgg 
      tttcttaaa tgtgcaatg gcggagact gcaatgtac 
      gcctatgct tggaacacc actgtctgt accacgaaa 
      attctcggt tttaattta acacgctgt atatgcccg 
      aaggtgggg accttcgcc ctcttgctc acagcaaac 
      aatattcag atcgaaaaa gaagagcca cgacccttg 
      cgatacatt gttctagcc ggttgctcc ctttgtttc 
      ttatcagca ctgataagt acaattatt ttgacgatt 
      cattggatt ttgaaacgc tcctgtgtt gtttttttg 
      aagctgcaa tgttccgtt tccataact ggggttatg 
      ctaactttt gtgcttgtc acaatcagc gatccttca 
      gagcggctc tttgcgagc gtgatggcc atcctggaa 
      atcctcttc ctcatgggt ctttccgcc cacatcagc 
      atcctcctc atggtctac accgaaatg gtccaattg 
      cccaaatca gcggcttcc aagcaaacc gtggtgggc 
      ataagcact ggggtgcca gtgattgcc gttttcggc 
      aaccatctg gcccaccag accatggac gtgcgcctc 
      aaatcctgg tggctgctg gctgggagc ctagccttc 
      aacattttc ctgaccgtg ggcgtcgta atcgtgggc 
      ctgttcgtc cttttgcac gtcacagtt ttgtacaaa 
      ttgaaaaaa ctgacaaca gtccacgaa aaagaagga 
      aaaatttta cagaagcgg attacgctc ctgaaaaaa 
      tcggcgtat gtgttctgt tcgctggtt tcggcgacc 
      gtcagcagt attttttac gtgaacttt gtaaatata 
      gtttggtgg cattacgag ttcagtgtt gtgacaatg 
      ttgctgggt ctggtcgtg gtcgtttgt tacgtcgtc 
      aaatcggat ggtgatttt ttttatttg ttgcaagga 
      cgattcaaa gccaaacaa aaggacggc tttttcggg 
      gtggaagct acaggtagt ccattaaat tttttcacc 
      aaagaagac gcggaggtg gagagcgag agcatcccc 
      caccaccgc aaatccgag ccgtcaaaa cccatcgaa 
      tgcttcccc atgtccgac tcgtgcaaa tccggtctt 
      ccggaatgc gtttccggt gcaatggag tcatacgcc 
      cacagcgac gccatctac tgcgcccag acctccctg 
      gagcgctac aaccggagc ccccaactc caccgcaaa 
      accgccccc ttcaaaatc gaggacgag gtcagatgc 
      cgctccccc gacatcatc gccaccaaa atatgcacc 
      caactcgac ctagtcgcc tcgcacgcc cccagcatc 
      gtcatcacc cccgaagag aggccgatg agggaaatc 
      gaacgggag attgataca gtttgcgaa aaaatcgaa 
      ctggagagt gggagtggg gctggggga gataatctg 
      gacgggatg ctggaccgg attagccac gatttggat 
      tatctgctg aatcggagc gaggagggg ccgaggagg 
      cactccaag actaatatt ataacggag cagatcatc 
      gaagaggtg gaggaggag ggcaaggtt ccggacgca 
      atcacggat attttgagg actaattgc tag
    mRNA sequence
    • >TC034482 RA
      cgccaccgc caccgccgc cccctgcag cagcagcag 
      ctagcgtcg agaggaagg ccctattcg caatacgac 
      gcgaaacgc cgttacgca cggttcaag gcaaactta 
      ctgcaaacg cggacgaac gagaaaata atacgaact 
      atttttcaa atttaatcc aatttttgt gaatctcga 
      tgttgtaaa gtgtgtttt tggaactag gtttaagtg 
      tgaggggac atgtgctag agtgagggc gattttagg 
      cacacactt gcccgatgc tcttcctcc tgccgctgc 
      tcctcgccg ccgccgccg gtggccgcc cccggtgcg 
      ggggcctgc tcaccgccg cccggggca tcctccaaa 
      ccccctcat tcccgtcgg aattccccg tccccatcc 
      actgcgaat gggtgatcg atgcccaaa atttcgcct 
      cccccaaca cctccatcg tggtttacc tcacccagc 
      tgtacgtcc acgagggtt tgaccttca ccgagtacc 
      aggtgtacg accgcactt accagctcg acggtcggg 
      agatccacc gcgtcaacg agaccaacg tggtgcagg 
      tgcgatggg tgcagtcgt tccagaatt ttttggtcg 
      tgaccttga agctggata gtgtggata gcgcccatc 
      tgagggtgc ttgatgggt ttctggacg tgtatgggt 
      ttaatatca cgtatgaaa tagcgcgag ggccggcca 
      gagtggact cgtgcacta ttatggatt gcggtttta 
      cggggattt gctacgacc attacacaa aattcagct 
      gcgagtgtt ttcccggct acagtggcc ctcattgtt 
      cttccggtc ccaactctt tctgtgcga acgcaaacc 
      caacgtgca aaaacggag gcacttgca tccatgtgg 
      gagtttccg ctgtgaaat gccactgcc agatgggct 
      tcactggcg agacatgcc aaattcccg tcaccacac 
      tgaagcccg gatgtgaca actgcgtcc agagctgtc 
      cgttcgatg ggaggctgg agaatgcct gcaagtgtc 
      cgatgatga acggaacta atgatcggg tcggattca 
      ccagcacca tgcatttgg ccaatctgc ccaatctga 
      gcggaaacg ccctcaaaa cgttcatcg aaaaccagg 
      tattcagga cgctcaaga ccaactact cgaaactgg 
      acgacgttt cggtgcaaa atgtcgcgt ttttcaggt 
      caggtgcag atgtaactt tccatttgt tcgcgctga 
      aaaaggacg agaaacgca tcagggcag ttttcagca 
      gatgggctg ataggggtt acatcgcca acttgacag 
      tcacagaca aggagtttg tcttcaagc cgatgctcg 
      atttacaga gcgtgtcaa tcaatcaaa tcgggccga 
      tccgtcaaa acgacgagt ttatccttt cgtgtgtgg 
      cgaggggtt cgcccacga cgtccttca gatggttca 
      aggacggcg tgtttgtca atgtgacgg aaattacaa 
      gtcacaaat ggaaccgtc tcataaaat atccccatt 
      cggatgatc aatacacgg ccctgttag gaatctcaa 
      ggagcgata ttttagaca gcggcacct tcacttgcc 
      aagttgagg attacggaa ttcaacaat gcctttcga 
      gaagagtcg ccatcaaag caccgccgg ttattaaag 
      tcgaaccta tgagtctca ccgtacgga aaggccaaa 
      atttaacta ttaagtgca ttagcatgg gcgacagca 
      acaacaaat acacttata gctggacca aaaacaaag 
      aactgattc ctgtcagga gcgagacgg agaagtacg 
      agattttgt atcctggag gaagcatct tgcaaatat 
      tcaaaatcg aaaaatcgg tgaaattct cctgcctgg 
      tgcaagacg gtccaattt cgagcgaaa ccagcgtcg 
      atatccacg tggtggacc cgtcctcgg tgcccacgt 
      gccccccgg agtattcct tgaacgtta catggccgg 
      aaacggccc ccgacaccg atagtctcc aggagtgtc 
      ccaaaggct acgcatttc cgggattcg tccggcgtt 
      cgtgcactc taaacgatg gcttacgtc ccatctggc 
      gtcagccgg attactccc aatgcactc ccgagcaat 
      tatacaaaa tagggttgg acctgagat tattgcaac 
      tgggctacg aaacagcaa cgccgctcc agatcatcc 
      aaaactgct ccagatacc taggggaga ggcaagggc 
      tgttgccgg gggagggca ccggggttt tggcaattt 
      tgagggacg tgattggtt attgtaaaa gggcaaggg 
      ttgtgacgg gcgatttgg tcaaggagg tgttcgaga 
      ttgtggatg gggtgttga ggcaccaga attcgctcg 
      tcagccaag agcaagtga agctcctgc aagaagtcg 
      tcagtcacc aaatcgaga tctctgcac ctctcctct 
      cgccctcgc agccccttt tccacttag cccttccaa 
      cccttgacg ttaatttaa tgtccggag atcaaaaag 
      aattaccat ttctctccg ccaacctca gtggccaca 
      aatggaccc cacacaaga ctcgaattt catccaaag 
      acccgaaac acggccaat gcgtgattg gagtggtca 
      tttaccgca atttgagcg cttttttac caacacggt 
      ccgtaatcc gagtaaaag acggagccg aagtggaat 
      acgaggtga tatctcaag tcgttcgct tatggatcc 
      tgtcacggg ataaaaatt acgattata atcctcaca 
      ttatcctcg agtggaaac agggaaatc acaacgcga 
      ccaaagacg gatggtttc ttaaatgtg caatggcgg 
      agactgcaa tgtacgcct atgcttgga acaccactg 
      tctgtacca cgaaaattc tcggtttta atttaacac 
      gctgtatat gcccgaagg tggggacct tcgccctct 
      tgctcacag caaacaata ttcagatcg aaaaagaag 
      agccacgac ccttgcgat acattgttc tagccggtt 
      gctcccttt gtttcttat cagcactga taagtacaa 
      ttattttga cgattcatt ggattttga aacgctcct 
      gtgttgttt ttttgaagc tgcaatgtt ccgtttcca 
      taactgggg ttatgctaa cttttgtgc ttgtcacaa 
      tcagcgatc cttcagagc ggctctttg cgagcgtga 
      tggccatcc tggaaatcc tcttcctca tgggtcttt 
      ccgcccaca tcagcatcc tcctcatgg tctacaccg 
      aaatggtcc aattgccca aatcagcgg cttccaagc 
      aaaccgtgg tgggcataa gcactgggg tgccagtga 
      ttgccgttt tcggcaacc atctggccc accagacca 
      tggacgtgc gcctcaaat cctggtggc tgctggctg 
      ggagcctag ccttcaaca ttttcctga ccgtgggcg 
      tcgtaatcg tgggcctgt tcgtccttt tgcacgtca 
      cagttttgt acaaattga aaaaactga caacagtcc 
      acgaaaaag aaggaaaaa ttttacaga agcggatta 
      cgctcctga aaaaatcgg cgtatgtgt tctgttcgc 
      tggtttcgg cgaccgtca gcagtattt tttacgtga 
      actttgtaa atatagttt ggtggcatt acgagttca 
      gtgttgtga caatgttgc tgggtctgg tcgtggtcg 
      tttgttacg tcgtcaaat cggatggtg atttttttt 
      atttgttgc aaggacgat tcaaagcca aacaaaagg 
      acggctttt tcggggtgg aagctacag gtagtccat 
      taaattttt tcaccaaag aagacgcgg aggtggaga 
      gcgagagca tcccccacc accgcaaat ccgagccgt 
      caaaaccca tcgaatgct tccccatgt ccgactcgt 
      gcaaatccg gtcttccgg aatgcgttt ccggtgcaa 
      tggagtcat acgcccaca gcgacgcca tctactgcg 
      cccagacct ccctggagc gctacaacc ggagccccc 
      aactccacc gcaaaaccg cccccttca aaatcgagg 
      acgaggtca gatgccgct cccccgaca tcatcgcca 
      ccaaaatat gcacccaac tcgacctag tcgcctcgc 
      acgccccca gcatcgtca tcacccccg aagagaggc 
      cgatgaggg aaatcgaac gggagattg atacagttt 
      gcgaaaaaa tcgaactgg agagtggga gtggggctg 
      ggggagata atctggacg ggatgctgg accggatta 
      gccacgatt tggattatc tgctgaatc ggagcgagg 
      aggggccga ggaggcact ccaagacta atattataa 
      cggagcaga tcatcgaag aggtggagg aggagggca 
      aggttccgg acgcaatca cggatattt tgaggacta 
      attgctaga aaaataggc aaattcggt tcgttcaag 
      accaaacca atcaatatc gcatcagat tgtacctga 
      aattctgtt acaataaat acgaattta tcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY