Status
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Gene information
General information
TC011620 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15578057..15581206
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011620
    Gene ontology

    GO terms for Tribolium

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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05343

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    iBeetle screen iB_06536

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011620 RA
      MTVINKMEV DGGPKGEGF RSYYITKIE ELQLIVAEK 
      SQNLRRLQA QRNELNAKV RMLREELML LQEQGSYVG 
      EVVKPMDKK KVLVKVHPE GKFVVDIDK NIDINDVTP 
      NCRVALRNE SYTLHKILP NKVDPLVSL MMVEKVPDS 
      TYEMVGGLD KQIKEIKEV IELPVKHPE LFDALGIAQ 
      PKGVLLYGP PGTGKTLLA RAVAHHTEC TFIRVSGSE 
      LVQKFIGEG SRMVRELFV MAREHAPSI IFMDEIDSI 
      GSSRIESGS GGDSEVQRT MLELLNQLD GFEATKNIK 
      VIMATNRID ILDPALLRP GRIDRKIEF PPPNEEARL 
      DILKIHSRK MNLTRGINL RKIAELMPG ASGAEVKGV 
      CTEAGMYAL RERRVHVTQ EDFEMAVAK VMQKDSEKN 
      MSIKKLWK
    CDS sequence
    • >TC011620 RA
      atgaccgtc ataaataag atggaagtg gacgggggg 
      cccaaggga gagggcttc cggtcgtat tacataacg 
      aaaatcgag gaattgcaa ctgattgtg gccgaaaaa 
      agccagaat ttgaggcgt ctccaagcg caaagaaac 
      gagctcaat gctaaagtg aggatgtta cgagaggaa 
      ctaatgctg ctccaagaa cagggaagt tacgtgggc 
      gaggttgtt aaacccatg gataagaaa aaagtccta 
      gttaaagta caccctgaa ggtaaattt gtggttgat 
      attgacaaa aacatcgac attaacgac gtgactccc 
      aattgtcgc gtcgcccta cgtaatgag agctacacc 
      ctgcacaaa attttgccc aacaaggtc gaccctctg 
      gtatcccta atgatggtg gaaaaagtc cccgactcg 
      acgtacgaa atggtcggc ggtttggac aaacagatc 
      aaagagatc aaagaagtg attgaactc ccagtgaaa 
      cacccggaa ttgttcgac gctttgggg atcgctcag 
      cccaaggga gtgctcctg tacggccct ccgggcacc 
      ggaaagacg ctcctggcc cgagctgtg gcccaccac 
      accgaatgc acctttatc cgggtttct ggctcggaa 
      ttggtccag aaattcatc ggggaaggg tcgcgaatg 
      gtgcgcgaa ttgttcgta atggcccga gaacacgcc 
      ccttcgatc atctttatg gacgagatt gattcaatc 
      gggtcgtcg aggattgaa tcgggctca gggggcgat 
      tctgaagtc caacgaaca atgttggaa ttgttgaat 
      caattggac gggtttgaa gcaacgaaa aatatcaaa 
      gttattatg gcgaccaat cgaattgat attttggat 
      ccggcactc ttaaggccg gggcgtatt gatcggaaa 
      attgagttt ccgccaccg aatgaagag gcgcggctt 
      gatattttg aaaatacat tcgagaaaa atgaatcta 
      acacgtggg attaatctg aggaaaatc gcagagctg 
      atgccgggc gccagtggg gccgaagtc aagggtgtc 
      tgcactgag gccgggatg tacgcctta agagagcgc 
      cgcgtccac gtgactcag gaggacttc gaaatggcc 
      gtagccaaa gtgatgcaa aaggattca gaaaagaac 
      atgtcgatt aaaaaattg tggaaataa
    mRNA sequence
    • >TC011620 RA
      gataaatgt tccggacga aaaaaaaac aattcactt 
      ataaattca tttatttaa ctaaaagcc cgctacaca 
      atttttaat ttacaaatt catcttaac gtccttgaa 
      ccctctgga ggcgtaatc cgagggctt ggtccgatc 
      ggacagctt cggatatcc gggatcccc ctcaaccac 
      ggcctccct tgatttaaa agccttcca tccgccggc 
      cctcgtcca atcattggc attatactt gaagtaata 
      agtgaacca ataggcggc cataacccc caagaaagc 
      cttcccagt gacgtatcg cacgttcct tgcccgttc 
      ttcccccaa aaccggaag caaaacctt ggccaactg 
      atccaaagg ccagcggta ggccctccg gatggggtt 
      gtaggtggc tttgtacat ttcgggaac cggtcgggg 
      ctcacttgg ggccaactc ttggtcttt gagccattg 
      tttacggaa cgctcgttc ctcgtctgt catgcccaa 
      gaggcgctc gcgctcgct gttgaagcg cccggcgat 
      ggacatggg cttgacgcc cccggtttg cgacgcttt 
      gcccatgct ttcacacaa acgaacaca aatgaattg 
      ttatataat ttggggtta ggtgtcaaa actgacagc 
      tgacgtttt tgcttcaaa aaaaaaaat taattaccg 
      aaattgagc ccttccctg cgccagtta cgcaagttt 
      aagtgaaaa aatgcaatt tttaattta ttcaatgcg 
      tgttggatt aatttaatt agaattatg ctggtttaa 
      ttttttgcg attgtcggc ctgtggctg tcagctgtc 
      aatattaaa acgtcactt gtgatgatt ttccaattg 
      tgctttgtt tgaagatta gtggcagtt tttgagtga 
      attagcgtc gtttttgtg ccgataagt tatcgcaaa 
      aggccgcga aaatgaccg tcataaata agatggaag 
      tggacgggg ggcccaagg gagagggct tccggtcgt 
      attacataa cgaaaatcg aggaattgc aactgattg 
      tggccgaaa aaagccaga atttgaggc gtctccaag 
      cgcaaagaa acgagctca atgctaaag tgaggatgt 
      tacgagagg aactaatgc tgctccaag aacagggaa 
      gttacgtgg gcgaggttg ttaaaccca tggataaga 
      aaaaagtcc tagttaaag tacaccctg aaggtaaat 
      ttgtggttg atattgaca aaaacatcg acattaacg 
      acgtgactc ccaattgtc gcgtcgccc tacgtaatg 
      agagctaca ccctgcaca aaattttgc ccaacaagg 
      tcgaccctc tggtatccc taatgatgg tggaaaaag 
      tccccgact cgacgtacg aaatggtcg gcggtttgg 
      acaaacaga tcaaagaga tcaaagaag tgattgaac 
      tcccagtga aacacccgg aattgttcg acgctttgg 
      ggatcgctc agcccaagg gagtgctcc tgtacggcc 
      ctccgggca ccggaaaga cgctcctgg cccgagctg 
      tggcccacc acaccgaat gcaccttta tccgggttt 
      ctggctcgg aattggtcc agaaattca tcggggaag 
      ggtcgcgaa tggtgcgcg aattgttcg taatggccc 
      gagaacacg ccccttcga tcatcttta tggacgaga 
      ttgattcaa tcgggtcgt cgaggattg aatcgggct 
      cagggggcg attctgaag tccaacgaa caatgttgg 
      aattgttga atcaattgg acgggtttg aagcaacga 
      aaaatatca aagttatta tggcgacca atcgaattg 
      atattttgg atccggcac tcttaaggc cggggcgta 
      ttgatcgga aaattgagt ttccgccac cgaatgaag 
      aggcgcggc ttgatattt tgaaaatac attcgagaa 
      aaatgaatc taacacgtg ggattaatc tgaggaaaa 
      tcgcagagc tgatgccgg gcgccagtg gggccgaag 
      tcaagggtg tctgcactg aggccggga tgtacgcct 
      taagagagc gccgcgtcc acgtgactc aggaggact 
      tcgaaatgg ccgtagcca aagtgatgc aaaaggatt 
      cagaaaaga acatgtcga ttaaaaaat tgtggaaat 
      aattttatt ttaataaaa attagtaac ttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY