General information | |||
TC011636 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15793403..15811794 | |
Sequence information | |||
Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC011636 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06532 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_09531 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC011636 RA
MEITSGVTG GIFLVILVI SLVLYRNWK YEQELDSLL WKVDFKDIQ INEEAGTKI TRSVHPLIR TSQVSLSSN PDADFRYST IFTQIGIYK GRVFAIKKV HKKSIDITR EMKKELKMM RDLRHDNLN AFIGACTDP PNICIVTEY CTRGSLKDI LENDDVKLD NMFIASLVG DILRGMIYL HDSPVRFHG ALHSANCLV DSRWVVKLA DFGLREFKK GAEEPGVKD PAKVREKCY RLLYKAPEL LRLQESFVS GQLGTQKGD IYSFGIILY ELHSRHGPF GTTELSYPE ILSKIIHNT GPQPFRPPL EALENSFDF VRDCLKECW AENPEDRPD FKSIRAKLR PLRKGMRPN IFDNMMAMM EKYANNLEV LVDERTDQL QEEKKKTEA LLYEMLPRP VAEQLKRGN KVEAESFDC VTIYFSDIV GFTAMSAES TPLQVVDFL NDLYTCFDS IIEHYDVYK VETIGDAYM VVSGLPIRN DNQHAAEIA SMSLHLLAE VKQFTIRHR PTEKLMLRI GIHSGPVCA GVVGLKMPR YCLFGDTVN TASRMESTG SPLKIHCSS ECKDLLHKL GGYNLMERG MVSMKGKGE QQTYWLLGE DPYFRNLRK EERSKRRTK TETKSLNTN GHFIITRSS LKNKNSIVR SPLPRCSSF ESPKKLRFA SGDNLEKKS DSNFLEVIS DNSPCKKSS NSLVENSLD CCSEAWKTS STSCPCIEN LANSAATLA QSQLSMLYK DEKNVKLVR PTCYSVPML CSLAVPRPV IHTISAPTS PRKQDLVDL TKFPECEEV IPWADSTPL LKITKPQDS ETGV
- >TC011636 RB
MALTETATE TFTIGYLTG SQRRPGDLE YPRPGLTIS GAISLAVDE VNKGIVGQR GHRLDFIVA ETYGEEVIS VQKTADLWT KNVSAYVGP QETCEHEAF MAAAFNLPM ISYFCTHYA TSDKTKFPT FARTRPPDT QISKSVASV LVAFNWTHV VLLYLKSPD FEFGNIATI VLQTLASAG ITVITTKYW DTPYHHGYG ENPFYKLVE QTYRDTRIF VILGHYYEH LGLMVAMEE KKLFDKGEY FVLGVDIEQ YDNENPSKY LRGLLRDET DPVAQKAFR NYLGVVPSS PVGFENFTI LVNSYMEKP PFNFPNPLI FVGGSKRIR AEAAYLYDA VHLYAKALI QVLDEGGNP RNGTDIIDH MKETHYKSA MGYIVYMDE NGDAEGNYT LIARKTSTG DEKFGLFPV GIFALKRTD SKLPILHLT DTIDWVGGQ PPIAAPICG FRGEKCITH TMEITSGVT GGIFLVILV ISLVLYRNW KYEQELDSL LWKVDFKDI QINEEAGTK ITRSVHPLI RTSQVSLSS NPDADFRYS TIFTQIGIY KGRVFAIKK VHKKSIDIT REMKKELKM MRDLRHDNL NAFIGACTD PPNICIVTE YCTRGSLKD ILENDDVKL DNMFIASLV GDILRGMIY LHDSPVRFH GALHSANCL VDSRWVVKL ADFGLREFK KGAEEPGVK DPAKVREKC YRLLYKAPE LLRLQESFV SGQLGTQKG DIYSFGIIL YELHSRHGP FGTTELSYP EILSKIIHN TGPQPFRPP LEALENSFD FVRDCLKEC WAENPEDRP DFKSIRAKL RPLRKGMRP NIFDNMMAM MEKYANNLE VLVDERTDQ LQEEKKKTE ALLYEMLPR PVAEQLKRG NKVEAESFD CVTIYFSDI VGFTAMSAE STPLQVVDF LNDLYTCFD SIIEHYDVY KVETIGDAY MVVSGLPIR NDNQHAAEI ASMSLHLLA EVKQFTIRH RPTEKLMLR IGIHSGPVC AGVVGLKMP RYCLFGDTV NTASRMEST GSPLKIHCS SECKDLLHK LGGYNLMER GMVSMKGKG EQQTYWLLG EDPYFRNLR KEERSKRRT KTETKSLNT NGHFIITRS SLKNKNSIV RSPLPRCSS FESPKKLRF ASGDNLEKK SDSNFLEVI SDNSPCKKS SNSLVENSL DCCSEAWKT SSTSCPCIE NLANSAATL AQSQLSMLY KDEKNVKLV RPTCYSVPM LCSLAVPRP VIHTISAPT SPRKQDLVD LTKFPECEE VIPWADSTP LLKITKPQD SETGV
- >TC011636 RC
MALTETATE TFTIGYLTG SQRRPGDLE YPRPGLTIS GAISLAVDE VNKGIVGQR GHRLDFIVA ETYGEEVIS VQKTADLWT KNVSAYVGP QETCEHEAF MAAAFNLPM ISYFCTHYA TSDKTKFPT FARTRPPDT QISKSVASV LVAFNWTHV VLLYLKSPD FEFGNIATI VLQTLASAG ITVITTKYW DTPYHHGYG ENPFYKLVE QTYRDTRIF VILGHYYEH LGLMVAMEE KKLFDKGEY FVLGVDIEQ YDNENPSKY LRGLLRDET DPVAQKAFR NYLGVVPSS PVGFENFTI LVNSYMEKP PFNFPNPLI FVGGSKRIR AEAAYLYDA VHLYAKALI QVLDEGGNP RNGTDIIDH MKETHYKSA MG
- >TC011636 RA
atggagata acgagcggt gtcaccggc ggcatattc ctggtgatc ctcgtgatc tccttagtg ctttaccgg aactggaaa tacgaacag gaactcgat agtctgctg tggaaagtc gatttcaaa gacatccaa atcaatgaa gaagccggg acgaaaata acccgatcg gttcatccg ctgataagg accagtcaa gtatcgctc agttctaat cccgatgct gattttcgc tattcgacc atttttacc cagattggg atttacaaa gggcgcgtt ttcgctatt aagaaggtt cataaaaaa tccattgat atcaccaga gagatgaag aaggaactt aaaatgatg cgcgacctc cgtcacgac aatctcaac gcttttatc ggcgcttgc accgaccct cctaacatt tgcatagtc accgaatat tgcacaaga ggcagtctt aaggacatc ctagagaac gacgacgtc aaactcgac aatatgttc atcgcttct ctagtcggg gacatttta aggggcatg atatacctt catgactcc cccgtccga tttcacggt gccttgcat tcagccaac tgcttagtg gattcgaga tgggtggtg aaattggcc gattttggc ctacgtgag ttcaagaaa ggagcggag gagcccgga gttaaagat ccggcaaaa gtccgggaa aaatgctac agattactg tacaaagct cccgaattg ctccgattg caagagtcg ttcgtttcg ggacagtta gggacccaa aaaggggat atttactcg ttcgggatc attttgtac gagcttcat agccgacat ggccctttc gggaccacg gaattatcc tatccggaa attctcagt aaaattata cacaataca ggaccacaa ccctttagg ccgccactt gaagcgcta gaaaatagt ttcgatttt gtgagagat tgtctgaag gagtgctgg gctgaaaac cccgaagat cggcccgat tttaagagc ataagggcc aaacttagg cctctacgc aagggaatg aggcccaac attttcgac aacatgatg gcgatgatg gagaaatac gccaacaat ttggaggtg ttggtggac gagcgcacg gatcaatta caagaagag aaaaagaaa acagaggct ttgttatac gaaatgttg cctcggcct gttgccgaa cagctgaaa agggggaac aaagtcgaa gctgaaagt ttcgattgt gtgacaatt tatttcagc gatattgtt ggctttaca gcgatgtcg gccgaaagc acaccctta caggttgtc gattttttg aacgatctt tacacttgt ttcgactca atcatcgaa cattacgac gtttacaaa gtggaaaca atcggagat gcctatatg gtggtcagc gggcttcca attcgcaac gacaatcaa catgcggct gaaattgcc tccatgtct ttacatctt ttagcggaa gttaaacag tttacgatc aggcatagg cccacggaa aaattaatg ctccgaatt gggattcat tcgggccca gtgtgcgcg ggtgtcgtc ggtctaaaa atgccccga tattgttta ttcggcgac actgtcaac accgcaagc cgcatggaa tcaacagga agccctttg aagattcat tgtagctcg gaatgcaaa gaccttttg cacaaactg ggcggttac aacctcatg gagcgcgga atggtctcc atgaaggga aaaggcgaa caacaaacg tactggctt ttgggggaa gacccctat ttccgtaac ttgcgcaag gaagagcgc tcaaaacgc cgcacaaaa accgagacc aaatcgcta aacaccaat ggtcacttc ataatcacg cgaagctcg ctcaaaaac aaaaacagc atagtgcgc agccccctt ccacggtgc tcctccttc gagagcccc aaaaaactg cgtttcgcc agtggtgac aacctggag aaaaaaagc gattccaat tttttggaa gtaatctcc gataatagt ccgtgcaaa aaatcctcc aattcgctc gttgagaat tcgctcgac tgttgcagc gaagcctgg aagacgtcc agcacttcg tgtccgtgt atcgaaaat ttagccaat tcggctgca actttggcc caatcgcag ctttcgatg ctgtacaaa gacgagaaa aacgttaaa ctggtcaga cctacgtgt tacagtgtt cccatgttg tgttcgttg gctgttccc cgaccggtg attcacacg atttcggcg cccactagc cccaggaag caggatttg gtcgatttg accaaattc ccagagtgt gaggaagtc atcccctgg gcggattcc acgcccttg ctcaaaatc accaaaccg caagactcg gaaactggt gtctag
- >TC011636 RB
atggcatta actgagacc gcaactgaa actttcaca attggatat ttgaccggt tctcagagg agacctggt gatttggaa taccccaga cctggtctg accatatct ggggccata tctctggca gtcgatgaa gtgaacaaa ggcattgtc ggccaacgt ggccacaga ctcgacttc atcgtggcc gaaacctac ggagaggaa gtgataagt gtacaaaaa acggctgat ttgtggacg aaaaatgtg tcggcatat gtggggccg caggaaacc tgcgaacat gaagctttc atggcggcg gctttcaat ctacccatg atttcatac ttttgcacc cattacgcc acttccgac aaaacgaaa tttccaact tttgcaaga acacgacca cctgataca caaatttcc aagtcggtg gcatctgtt ctcgttgca tttaattgg acacatgtg gttttattg tatttgaaa tcgccggac tttgagttt ggaaatatt gccaccata gttttgcaa actctagcc tcggctgga attacagta atcacgacg aaatattgg gacactcct tatcaccac ggctacggg gagaaccct ttctataaa ttggtcgaa cagacctac agggacacc agaatcttc gtcattttg ggtcactat tacgaacat ttgggacta atggtggca atggaagag aagaaattg ttcgataaa ggcgagtat ttcgtcctg ggtgtggat atcgaacaa tacgacaac gagaatcca tcgaaatat ttacgaggc cttcttcga gacgaaact gatccggtg gctcaaaaa gccttcagg aattatcta ggagtggtt ccgtccagt ccggtaggc ttcgaaaac ttcacaata cttgtgaat tcttatatg gaaaagcca ccgtttaat tttccgaat cctcttatc ttcgtcggc ggatccaaa cggattaga gctgaagcg gcttattta tacgacgcc gtgcacctt tacgccaaa gctttgatt caagttttg gacgaaggg gggaatccc aggaacggt acggatata attgaccac atgaaggag acgcattat aagagtgca atgggatat atcgtctat atggacgaa aacggagac gccgaaggc aactacact ctaatcgcc cgcaaaacc agcaccggt gacgaaaaa ttcggcctc ttccccgtc ggcatcttc gccttgaaa cgaaccgac tccaaacta ccaattctt cacctgact gatacaata gactgggtc ggaggtcag cctccaatc gcagccccg atttgcggt tttcgcggc gaaaaatgc atcacccat actatggag ataacgagc ggtgtcacc ggcggcata ttcctggtg atcctcgtg atctcctta gtgctttac cggaactgg aaatacgaa caggaactc gatagtctg ctgtggaaa gtcgatttc aaagacatc caaatcaat gaagaagcc gggacgaaa ataacccga tcggttcat ccgctgata aggaccagt caagtatcg ctcagttct aatcccgat gctgatttt cgctattcg accattttt acccagatt gggatttac aaagggcgc gttttcgct attaagaag gttcataaa aaatccatt gatatcacc agagagatg aagaaggaa cttaaaatg atgcgcgac ctccgtcac gacaatctc aacgctttt atcggcgct tgcaccgac cctcctaac atttgcata gtcaccgaa tattgcaca agaggcagt cttaaggac atcctagag aacgacgac gtcaaactc gacaatatg ttcatcgct tctctagtc ggggacatt ttaaggggc atgatatac cttcatgac tcccccgtc cgatttcac ggtgccttg cattcagcc aactgctta gtggattcg agatgggtg gtgaaattg gccgatttt ggcctacgt gagttcaag aaaggagcg gaggagccc ggagttaaa gatccggca aaagtccgg gaaaaatgc tacagatta ctgtacaaa gctcccgaa ttgctccga ttgcaagag tcgttcgtt tcgggacag ttagggacc caaaaaggg gatatttac tcgttcggg atcattttg tacgagctt catagccga catggccct ttcgggacc acggaatta tcctatccg gaaattctc agtaaaatt atacacaat acaggacca caacccttt aggccgcca cttgaagcg ctagaaaat agtttcgat tttgtgaga gattgtctg aaggagtgc tgggctgaa aaccccgaa gatcggccc gattttaag agcataagg gccaaactt aggcctcta cgcaaggga atgaggccc aacattttc gacaacatg atggcgatg atggagaaa tacgccaac aatttggag gtgttggtg gacgagcgc acggatcaa ttacaagaa gagaaaaag aaaacagag gctttgtta tacgaaatg ttgcctcgg cctgttgcc gaacagctg aaaaggggg aacaaagtc gaagctgaa agtttcgat tgtgtgaca atttatttc agcgatatt gttggcttt acagcgatg tcggccgaa agcacaccc ttacaggtt gtcgatttt ttgaacgat ctttacact tgtttcgac tcaatcatc gaacattac gacgtttac aaagtggaa acaatcgga gatgcctat atggtggtc agcgggctt ccaattcgc aacgacaat caacatgcg gctgaaatt gcctccatg tctttacat cttttagcg gaagttaaa cagtttacg atcaggcat aggcccacg gaaaaatta atgctccga attgggatt cattcgggc ccagtgtgc gcgggtgtc gtcggtcta aaaatgccc cgatattgt ttattcggc gacactgtc aacaccgca agccgcatg gaatcaaca ggaagccct ttgaagatt cattgtagc tcggaatgc aaagacctt ttgcacaaa ctgggcggt tacaacctc atggagcgc ggaatggtc tccatgaag ggaaaaggc gaacaacaa acgtactgg cttttgggg gaagacccc tatttccgt aacttgcgc aaggaagag cgctcaaaa cgccgcaca aaaaccgag accaaatcg ctaaacacc aatggtcac ttcataatc acgcgaagc tcgctcaaa aacaaaaac agcatagtg cgcagcccc cttccacgg tgctcctcc ttcgagagc cccaaaaaa ctgcgtttc gccagtggt gacaacctg gagaaaaaa agcgattcc aattttttg gaagtaatc tccgataat agtccgtgc aaaaaatcc tccaattcg ctcgttgag aattcgctc gactgttgc agcgaagcc tggaagacg tccagcact tcgtgtccg tgtatcgaa aatttagcc aattcggct gcaactttg gcccaatcg cagctttcg atgctgtac aaagacgag aaaaacgtt aaactggtc agacctacg tgttacagt gttcccatg ttgtgttcg ttggctgtt ccccgaccg gtgattcac acgatttcg gcgcccact agccccagg aagcaggat ttggtcgat ttgaccaaa ttcccagag tgtgaggaa gtcatcccc tgggcggat tccacgccc ttgctcaaa atcaccaaa ccgcaagac tcggaaact ggtgtctag
- >TC011636 RC
atggcatta actgagacc gcaactgaa actttcaca attggatat ttgaccggt tctcagagg agacctggt gatttggaa taccccaga cctggtctg accatatct ggggccata tctctggca gtcgatgaa gtgaacaaa ggcattgtc ggccaacgt ggccacaga ctcgacttc atcgtggcc gaaacctac ggagaggaa gtgataagt gtacaaaaa acggctgat ttgtggacg aaaaatgtg tcggcatat gtggggccg caggaaacc tgcgaacat gaagctttc atggcggcg gctttcaat ctacccatg atttcatac ttttgcacc cattacgcc acttccgac aaaacgaaa tttccaact tttgcaaga acacgacca cctgataca caaatttcc aagtcggtg gcatctgtt ctcgttgca tttaattgg acacatgtg gttttattg tatttgaaa tcgccggac tttgagttt ggaaatatt gccaccata gttttgcaa actctagcc tcggctgga attacagta atcacgacg aaatattgg gacactcct tatcaccac ggctacggg gagaaccct ttctataaa ttggtcgaa cagacctac agggacacc agaatcttc gtcattttg ggtcactat tacgaacat ttgggacta atggtggca atggaagag aagaaattg ttcgataaa ggcgagtat ttcgtcctg ggtgtggat atcgaacaa tacgacaac gagaatcca tcgaaatat ttacgaggc cttcttcga gacgaaact gatccggtg gctcaaaaa gccttcagg aattatcta ggagtggtt ccgtccagt ccggtaggc ttcgaaaac ttcacaata cttgtgaat tcttatatg gaaaagcca ccgtttaat tttccgaat cctcttatc ttcgtcggc ggatccaaa cggattaga gctgaagcg gcttattta tacgacgcc gtgcacctt tacgccaaa gctttgatt caagttttg gacgaaggg gggaatccc aggaacggt acggatata attgaccac atgaaggag acgcattat aagagtgca atggggtaa
- >TC011636 RA
tcggaggtc agcctccaa tcgcagccc cgatttgcg gttttcgcg gcgaaaaat gcatcaccc atactatgg agataacga gcggtgtca ccggcggca tattcctgg tgatcctcg tgatctcct tagtgcttt accggaact ggaaatacg aacaggaac tcgatagtc tgctgtgga aagtcgatt tcaaagaca tccaaatca atgaagaag ccgggacga aaataaccc gatcggttc atccgctga taaggacca gtcaagtat cgctcagtt ctaatcccg atgctgatt ttcgctatt cgaccattt ttacccaga ttgggattt acaaagggc gcgttttcg ctattaaga aggttcata aaaaatcca ttgatatca ccagagaga tgaagaagg aacttaaaa tgatgcgcg acctccgtc acgacaatc tcaacgctt ttatcggcg cttgcaccg accctccta acatttgca tagtcaccg aatattgca caagaggca gtcttaagg acatcctag agaacgacg acgtcaaac tcgacaata tgttcatcg cttctctag tcggggaca ttttaaggg gcatgatat accttcatg actcccccg tccgatttc acggtgcct tgcattcag ccaactgct tagtggatt cgagatggg tggtgaaat tggccgatt ttggcctac gtgagttca agaaaggag cggaggagc ccggagtta aagatccgg caaaagtcc gggaaaaat gctacagat tactgtaca aagctcccg aattgctcc gattgcaag agtcgttcg tttcgggac agttaggga cccaaaaag gggatattt actcgttcg ggatcattt tgtacgagc ttcatagcc gacatggcc ctttcggga ccacggaat tatcctatc cggaaattc tcagtaaaa ttatacaca atacaggac cacaaccct ttaggccgc cacttgaag cgctagaaa atagtttcg attttgtga gagattgtc tgaaggagt gctgggctg aaaaccccg aagatcggc ccgatttta agagcataa gggccaaac ttaggcctc tacgcaagg gaatgaggc ccaacattt tcgacaaca tgatggcga tgatggaga aatacgcca acaatttgg aggtgttgg tggacgagc gcacggatc aattacaag aagagaaaa agaaaacag aggctttgt tatacgaaa tgttgcctc ggcctgttg ccgaacagc tgaaaaggg ggaacaaag tcgaagctg aaagtttcg attgtgtga caatttatt tcagcgata ttgttggct ttacagcga tgtcggccg aaagcacac ccttacagg ttgtcgatt ttttgaacg atctttaca cttgtttcg actcaatca tcgaacatt acgacgttt acaaagtgg aaacaatcg gagatgcct atatggtgg tcagcgggc ttccaattc gcaacgaca atcaacatg cggctgaaa ttgcctcca tgtctttac atcttttag cggaagtta aacagttta cgatcaggc ataggccca cggaaaaat taatgctcc gaattggga ttcattcgg gcccagtgt gcgcgggtg tcgtcggtc taaaaatgc cccgatatt gtttattcg gcgacactg tcaacaccg caagccgca tggaatcaa caggaagcc ctttgaaga ttcattgta gctcggaat gcaaagacc ttttgcaca aactgggcg gttacaacc tcatggagc gcggaatgg tctccatga agggaaaag gcgaacaac aaacgtact ggcttttgg gggaagacc cctatttcc gtaacttgc gcaaggaag agcgctcaa aacgccgca caaaaaccg agaccaaat cgctaaaca ccaatggtc acttcataa tcacgcgaa gctcgctca aaaacaaaa acagcatag tgcgcagcc cccttccac ggtgctcct ccttcgaga gccccaaaa aactgcgtt tcgccagtg gtgacaacc tggagaaaa aaagcgatt ccaattttt tggaagtaa tctccgata atagtccgt gcaaaaaat cctccaatt cgctcgttg agaattcgc tcgactgtt gcagcgaag cctggaaga cgtccagca cttcgtgtc cgtgtatcg aaaatttag ccaattcgg ctgcaactt tggcccaat cgcagcttt cgatgctgt acaaagacg agaaaaacg ttaaactgg tcagaccta cgtgttaca gtgttccca tgttgtgtt cgttggctg ttccccgac cggtgattc acacgattt cggcgccca ctagcccca ggaagcagg atttggtcg atttgacca aattcccag agtgtgagg aagtcatcc cctgggcgg attccacgc ccttgctca aaatcacca aaccgcaag actcggaaa ctggtgtct agtcatttt ttatgtttg gatttaagg ctcgtgtta tttctattt attgatgtt tgttgtaat aaacgtgcg tatcggat
- >TC011636 RB
cacgtggac cagtttaaa tcaattcga taaaaatta caaaaaaaa tcattgtca gtgagatgc tcctacttg tcattctcg caatggcat taactgaga ccgcaactg aaactttca caattggat atttgaccg gttctcaga ggagacctg gtgatttgg aatacccca gacctggtc tgaccatat ctggggcca tatctctgg cagtcgatg aagtgaaca aaggcattg tcggccaac gtggccaca gactcgact tcatcgtgg ccgaaacct acggagagg aagtgataa gtgtacaaa aaacggctg atttgtgga cgaaaaatg tgtcggcat atgtggggc cgcaggaaa cctgcgaac atgaagctt tcatggcgg cggctttca atctaccca tgatttcat acttttgca cccattacg ccacttccg acaaaacga aatttccaa cttttgcaa gaacacgac cacctgata cacaaattt ccaagtcgg tggcatctg ttctcgttg catttaatt ggacacatg tggttttat tgtatttga aatcgccgg actttgagt ttggaaata ttgccacca tagttttgc aaactctag cctcggctg gaattacag taatcacga cgaaatatt gggacactc cttatcacc acggctacg gggagaacc ctttctata aattggtcg aacagacct acagggaca ccagaatct tcgtcattt tgggtcact attacgaac atttgggac taatggtgg caatggaag agaagaaat tgttcgata aaggcgagt atttcgtcc tgggtgtgg atatcgaac aatacgaca acgagaatc catcgaaat atttacgag gccttcttc gagacgaaa ctgatccgg tggctcaaa aagccttca ggaattatc taggagtgg ttccgtcca gtccggtag gcttcgaaa acttcacaa tacttgtga attcttata tggaaaagc caccgttta attttccga atcctctta tcttcgtcg gcggatcca aacggatta gagctgaag cggcttatt tatacgacg ccgtgcacc tttacgcca aagctttga ttcaagttt tggacgaag gggggaatc ccaggaacg gtacggata taattgacc acatgaagg agacgcatt ataagagtg caatgggat atatcgtct atatggacg aaaacggag acgccgaag gcaactaca ctctaatcg cccgcaaaa ccagcaccg gtgacgaaa aattcggcc tcttccccg tcggcatct tcgccttga aacgaaccg actccaaac taccaattc ttcacctga ctgatacaa tagactggg tcggaggtc agcctccaa tcgcagccc cgatttgcg gttttcgcg gcgaaaaat gcatcaccc atactatgg agataacga gcggtgtca ccggcggca tattcctgg tgatcctcg tgatctcct tagtgcttt accggaact ggaaatacg aacaggaac tcgatagtc tgctgtgga aagtcgatt tcaaagaca tccaaatca atgaagaag ccgggacga aaataaccc gatcggttc atccgctga taaggacca gtcaagtat cgctcagtt ctaatcccg atgctgatt ttcgctatt cgaccattt ttacccaga ttgggattt acaaagggc gcgttttcg ctattaaga aggttcata aaaaatcca ttgatatca ccagagaga tgaagaagg aacttaaaa tgatgcgcg acctccgtc acgacaatc tcaacgctt ttatcggcg cttgcaccg accctccta acatttgca tagtcaccg aatattgca caagaggca gtcttaagg acatcctag agaacgacg acgtcaaac tcgacaata tgttcatcg cttctctag tcggggaca ttttaaggg gcatgatat accttcatg actcccccg tccgatttc acggtgcct tgcattcag ccaactgct tagtggatt cgagatggg tggtgaaat tggccgatt ttggcctac gtgagttca agaaaggag cggaggagc ccggagtta aagatccgg caaaagtcc gggaaaaat gctacagat tactgtaca aagctcccg aattgctcc gattgcaag agtcgttcg tttcgggac agttaggga cccaaaaag gggatattt actcgttcg ggatcattt tgtacgagc ttcatagcc gacatggcc ctttcggga ccacggaat tatcctatc cggaaattc tcagtaaaa ttatacaca atacaggac cacaaccct ttaggccgc cacttgaag cgctagaaa atagtttcg attttgtga gagattgtc tgaaggagt gctgggctg aaaaccccg aagatcggc ccgatttta agagcataa gggccaaac ttaggcctc tacgcaagg gaatgaggc ccaacattt tcgacaaca tgatggcga tgatggaga aatacgcca acaatttgg aggtgttgg tggacgagc gcacggatc aattacaag aagagaaaa agaaaacag aggctttgt tatacgaaa tgttgcctc ggcctgttg ccgaacagc tgaaaaggg ggaacaaag tcgaagctg aaagtttcg attgtgtga caatttatt tcagcgata ttgttggct ttacagcga tgtcggccg aaagcacac ccttacagg ttgtcgatt ttttgaacg atctttaca cttgtttcg actcaatca tcgaacatt acgacgttt acaaagtgg aaacaatcg gagatgcct atatggtgg tcagcgggc ttccaattc gcaacgaca atcaacatg cggctgaaa ttgcctcca tgtctttac atcttttag cggaagtta aacagttta cgatcaggc ataggccca cggaaaaat taatgctcc gaattggga ttcattcgg gcccagtgt gcgcgggtg tcgtcggtc taaaaatgc cccgatatt gtttattcg gcgacactg tcaacaccg caagccgca tggaatcaa caggaagcc ctttgaaga ttcattgta gctcggaat gcaaagacc ttttgcaca aactgggcg gttacaacc tcatggagc gcggaatgg tctccatga agggaaaag gcgaacaac aaacgtact ggcttttgg gggaagacc cctatttcc gtaacttgc gcaaggaag agcgctcaa aacgccgca caaaaaccg agaccaaat cgctaaaca ccaatggtc acttcataa tcacgcgaa gctcgctca aaaacaaaa acagcatag tgcgcagcc cccttccac ggtgctcct ccttcgaga gccccaaaa aactgcgtt tcgccagtg gtgacaacc tggagaaaa aaagcgatt ccaattttt tggaagtaa tctccgata atagtccgt gcaaaaaat cctccaatt cgctcgttg agaattcgc tcgactgtt gcagcgaag cctggaaga cgtccagca cttcgtgtc cgtgtatcg aaaatttag ccaattcgg ctgcaactt tggcccaat cgcagcttt cgatgctgt acaaagacg agaaaaacg ttaaactgg tcagaccta cgtgttaca gtgttccca tgttgtgtt cgttggctg ttccccgac cggtgattc acacgattt cggcgccca ctagcccca ggaagcagg atttggtcg atttgacca aattcccag agtgtgagg aagtcatcc cctgggcgg attccacgc ccttgctca aaatcacca aaccgcaag actcggaaa ctggtgtct agtcatttt ttatgtttg gatttaagg ctcgtgtta tttctattt attgatgtt tgttgtaat aaacgtgcg tatcggat
- >TC011636 RC
cacgtggac cagtttaaa tcaattcga taaaaatta caaaaaaaa tcattgtca gtgagatgc tcctacttg tcattctcg caatggcat taactgaga ccgcaactg aaactttca caattggat atttgaccg gttctcaga ggagacctg gtgatttgg aatacccca gacctggtc tgaccatat ctggggcca tatctctgg cagtcgatg aagtgaaca aaggcattg tcggccaac gtggccaca gactcgact tcatcgtgg ccgaaacct acggagagg aagtgataa gtgtacaaa aaacggctg atttgtgga cgaaaaatg tgtcggcat atgtggggc cgcaggaaa cctgcgaac atgaagctt tcatggcgg cggctttca atctaccca tgatttcat acttttgca cccattacg ccacttccg acaaaacga aatttccaa cttttgcaa gaacacgac cacctgata cacaaattt ccaagtcgg tggcatctg ttctcgttg catttaatt ggacacatg tggttttat tgtatttga aatcgccgg actttgagt ttggaaata ttgccacca tagttttgc aaactctag cctcggctg gaattacag taatcacga cgaaatatt gggacactc cttatcacc acggctacg gggagaacc ctttctata aattggtcg aacagacct acagggaca ccagaatct tcgtcattt tgggtcact attacgaac atttgggac taatggtgg caatggaag agaagaaat tgttcgata aaggcgagt atttcgtcc tgggtgtgg atatcgaac aatacgaca acgagaatc catcgaaat atttacgag gccttcttc gagacgaaa ctgatccgg tggctcaaa aagccttca ggaattatc taggagtgg ttccgtcca gtccggtag gcttcgaaa acttcacaa tacttgtga attcttata tggaaaagc caccgttta attttccga atcctctta tcttcgtcg gcggatcca aacggatta gagctgaag cggcttatt tatacgacg ccgtgcacc tttacgcca aagctttga ttcaagttt tggacgaag gggggaatc ccaggaacg gtacggata taattgacc acatgaagg agacgcatt ataagagtg caatggggt aagtaatat ttttttgag aatattatt gtttcgctc tgaagtgat tttaattaa acgaacatc ctttga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
