General information | |||
TC011639 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15845863..15861627 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC011639 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06531 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC011639 RA
MRETLNASA CAALLDAVN WGDPQLLLS LSILALINV MVIVGNCLV IAAVFCSSK LRSVTNLFI VSLAVADLL VGVAVLPFS ATWEVFKVW IFGDVWCNM WLALDVWMC TASILNLCA ISLDRYVAV TRPITYPSI MSHSRAKLL IAGLWVLSF VICFPPLVG WKETERRCP WKCELTNEA GYVVYSALG SFYIPMFVM LFFYWRIYR AAIRTTRAI NQGFRTTKG SRGFGNRFD EQRLTLRIH RGRGSSDKR PHGSPHSNG SNSTTTSSV SPSPSTRGK HERVKISVS YPSSENLSP NPNLLAPAS PTQFAVHYS VNGKDTTTA LYKRENQLR VQRLSSRRR ASRRSSSGD SERAPSPSP SACEEGKKV MSRRMGKRN IKAQVKRFR METKAAKTL AIIVGGFIF CWLPFFTMY LVRAFCPDC IPPLLFSIL FWLGYCNSA INPLIYALF SKDFRFAFK RIICKCFCK GRDGGGRRG SDGSQLQGR QFRSPSYNV QQQGNSLGD DSDPGADPS DSR
- >TC011639 RA
atgcgagaa acactcaac gcatccgcg tgtgcggcg cttctcgat gctgtcaat tggggggat ccccaatta cttctctcc ttatcaatc ctagctctt atcaatgtt atggtcatt gtgggcaac tgtttggtc atagctgcc gttttctgc tcctccaaa ctgcgatca gtgacgaac ttgttcatc gtttctttg gctgttgcc gatttacta gtcggagtt gcagttttg cccttttcg gcgacttgg gaagttttt aaggtctgg atattcggc gacgtttgg tgcaacatg tggctggct ttggacgtc tggatgtgc accgcttcc attctcaat ttgtgtgcc atttctctg gaccgttat gtggccgtc acgcgtccc atcacatat cccagcatc atgtcccat tccagagcc aagctctta atcgctggc ttatgggtg ctcagtttt gttatctgt tttccgccg ctggtcggg tggaaagag actgaaaga cgttgtcct tggaagtgc gaactgact aacgaggca gggtatgtt gtgtattca gcccttggt agcttttac ataccgatg tttgtcatg ttgttcttc tactggagg atttatcga gcggctata agaaccacc agggcaatt aaccaagga tttaggacg accaaaggt tctcgcggt ttcggcaac cgtttcgac gagcaacgt ctcacgttg cgcattcat cgaggccgt ggctcttca gacaaacgt ccccacggc tcccctcac agcaacggc agcaacagc accaccaca agcagcgtc agccccagt ccttccact cgaggcaaa cacgagcgc gtcaaaatc agcgtcagt tatccctcc agcgaaaac ctttcgcct aatccaaac ctcctagct cctgcctca cccacacag ttcgccgtt cattactcc gtgaacgga aaggacacc accacggcg ttgtacaaa cgcgagaac cagctgaga gtgcagaga ctgagctcc aggaggagg gcctccagg aggtcaagc agtggcgat agcgaacgc gcaccaagt ccgagtccg agcgcttgt gaagaaggg aagaaggtg atgtcgagg agaatgggc aagaggaac atcaaggcg caagtgaag aggtttcgc atggagaca aaggcggcc aaaacgctg gcaatcatc gtcggaggt tttatcttc tgttggctt ccgtttttc accatgtat ctagtgcgg gcgttttgt cccgattgc attccaccg ttgctcttc tccatactc ttctggctg gggtattgc aacagtgcc atcaatccg ctcatttat gccctcttc tccaaagac ttcagattc gccttcaag cgcatcatc tgcaagtgc ttctgcaag ggtcgcgat ggaggcggt cgtcgcggt tccgacggt tcgcagctc caaggccgc cagttccga tctccaagc tacaacgtg cagcagcag ggaaattcg cttggagac gacagtgat ccaggcgca gatccgtcg gattcgagg tga
- >TC011639 RA
tcagtagca cggcgtcgt ccgaagtgt tcgctcacg tcgcgacgc gcctaaagc gctcgtaat tccagtgat tttcattca aaccttatg ctggcccac actgcacca caaagggtt tcccaatta agaagatga ggtcgtctc gagataaac aagcagcga aggcattat ggaaacgac gaacgatct aacgagatt accgtgtct gtgttgtaa ataatttcc tttcgagga gaatgtctg tgattcggt cgatttaat tttcggtgg tgatggtgc taagacctc gaaaagtgc taatgtgat gcgagaaac actcaacgc atccgcgtg tgcggcgct tctcgatgc tgtcaattg gggggatcc ccaattact tctctcctt atcaatcct agctcttat caatgttat ggtcattgt gggcaactg tttggtcat agctgccgt tttctgctc ctccaaact gcgatcagt gacgaactt gttcatcgt ttctttggc tgttgccga tttactagt cggagttgc agttttgcc cttttcggc gacttggga agtttttaa ggtctggat attcggcga cgtttggtg caacatgtg gctggcttt ggacgtctg gatgtgcac cgcttccat tctcaattt gtgtgccat ttctctgga ccgttatgt ggccgtcac gcgtcccat cacatatcc cagcatcat gtcccattc cagagccaa gctcttaat cgctggctt atgggtgct cagttttgt tatctgttt tccgccgct ggtcgggtg gaaagagac tgaaagacg ttgtccttg gaagtgcga actgactaa cgaggcagg gtatgttgt gtattcagc ccttggtag cttttacat accgatgtt tgtcatgtt gttcttcta ctggaggat ttatcgagc ggctataag aaccaccag ggcaattaa ccaaggatt taggacgac caaaggttc tcgcggttt cggcaaccg tttcgacga gcaacgtct cacgttgcg cattcatcg aggccgtgg ctcttcaga caaacgtcc ccacggctc ccctcacag caacggcag caacagcac caccacaag cagcgtcag ccccagtcc ttccactcg aggcaaaca cgagcgcgt caaaatcag cgtcagtta tccctccag cgaaaacct ttcgcctaa tccaaacct cctagctcc tgcctcacc cacacagtt cgccgttca ttactccgt gaacggaaa ggacaccac cacggcgtt gtacaaacg cgagaacca gctgagagt gcagagact gagctccag gaggagggc ctccaggag gtcaagcag tggcgatag cgaacgcgc accaagtcc gagtccgag cgcttgtga agaagggaa gaaggtgat gtcgaggag aatgggcaa gaggaacat caaggcgca agtgaagag gtttcgcat ggagacaaa ggcggccaa aacgctggc aatcatcgt cggaggttt tatcttctg ttggcttcc gtttttcac catgtatct agtgcgggc gttttgtcc cgattgcat tccaccgtt gctcttctc catactctt ctggctggg gtattgcaa cagtgccat caatccgct catttatgc cctcttctc caaagactt cagattcgc cttcaagcg catcatctg caagtgctt ctgcaaggg tcgcgatgg aggcggtcg tcgcggttc cgacggttc gcagctcca aggccgcca gttccgatc tccaagcta caacgtgca gcagcaggg aaattcgct tggagacga cagtgatcc aggcgcaga tccgtcgga ttcgaggtg acgtaaccg cagacggca cgtttcaca aatgtacct ggacaaaaa ctcggtttt taaagtggc catgtgttt gcacggctg acgaagaaa cctcgccgg agatggcga ccacatcta gacatacta gtctagcat ttatattta ttcgttctt ttttggaga gggaaagat taacgactt aactctttt aggataatt ttaataatg taagacttc atcacgcac aacggttta ttttttaat ttattgatt cgtgcattt gtacattct catcatcgc gaccgataa taaaggctg tgtttattg tagccaaaa atgttaggt aatttttta tgtcatcag atttgatgt ctaatattt aacaacact acctgtcat agagtgtgg aggagaaaa agacaaatt atttaaaca atgttacat atttgttaa aactgtgtt cgacaatgt actctcaat gtaacaagt gtaaataaa acatatacg gaaca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
