Status
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Gene information
General information
TC011639 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15845863..15861627
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011639
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06531

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011639 RA
      MRETLNASA CAALLDAVN WGDPQLLLS LSILALINV 
      MVIVGNCLV IAAVFCSSK LRSVTNLFI VSLAVADLL 
      VGVAVLPFS ATWEVFKVW IFGDVWCNM WLALDVWMC 
      TASILNLCA ISLDRYVAV TRPITYPSI MSHSRAKLL 
      IAGLWVLSF VICFPPLVG WKETERRCP WKCELTNEA 
      GYVVYSALG SFYIPMFVM LFFYWRIYR AAIRTTRAI 
      NQGFRTTKG SRGFGNRFD EQRLTLRIH RGRGSSDKR 
      PHGSPHSNG SNSTTTSSV SPSPSTRGK HERVKISVS 
      YPSSENLSP NPNLLAPAS PTQFAVHYS VNGKDTTTA 
      LYKRENQLR VQRLSSRRR ASRRSSSGD SERAPSPSP 
      SACEEGKKV MSRRMGKRN IKAQVKRFR METKAAKTL 
      AIIVGGFIF CWLPFFTMY LVRAFCPDC IPPLLFSIL 
      FWLGYCNSA INPLIYALF SKDFRFAFK RIICKCFCK 
      GRDGGGRRG SDGSQLQGR QFRSPSYNV QQQGNSLGD 
      DSDPGADPS DSR
    CDS sequence
    • >TC011639 RA
      atgcgagaa acactcaac gcatccgcg tgtgcggcg 
      cttctcgat gctgtcaat tggggggat ccccaatta 
      cttctctcc ttatcaatc ctagctctt atcaatgtt 
      atggtcatt gtgggcaac tgtttggtc atagctgcc 
      gttttctgc tcctccaaa ctgcgatca gtgacgaac 
      ttgttcatc gtttctttg gctgttgcc gatttacta 
      gtcggagtt gcagttttg cccttttcg gcgacttgg 
      gaagttttt aaggtctgg atattcggc gacgtttgg 
      tgcaacatg tggctggct ttggacgtc tggatgtgc 
      accgcttcc attctcaat ttgtgtgcc atttctctg 
      gaccgttat gtggccgtc acgcgtccc atcacatat 
      cccagcatc atgtcccat tccagagcc aagctctta 
      atcgctggc ttatgggtg ctcagtttt gttatctgt 
      tttccgccg ctggtcggg tggaaagag actgaaaga 
      cgttgtcct tggaagtgc gaactgact aacgaggca 
      gggtatgtt gtgtattca gcccttggt agcttttac 
      ataccgatg tttgtcatg ttgttcttc tactggagg 
      atttatcga gcggctata agaaccacc agggcaatt 
      aaccaagga tttaggacg accaaaggt tctcgcggt 
      ttcggcaac cgtttcgac gagcaacgt ctcacgttg 
      cgcattcat cgaggccgt ggctcttca gacaaacgt 
      ccccacggc tcccctcac agcaacggc agcaacagc 
      accaccaca agcagcgtc agccccagt ccttccact 
      cgaggcaaa cacgagcgc gtcaaaatc agcgtcagt 
      tatccctcc agcgaaaac ctttcgcct aatccaaac 
      ctcctagct cctgcctca cccacacag ttcgccgtt 
      cattactcc gtgaacgga aaggacacc accacggcg 
      ttgtacaaa cgcgagaac cagctgaga gtgcagaga 
      ctgagctcc aggaggagg gcctccagg aggtcaagc 
      agtggcgat agcgaacgc gcaccaagt ccgagtccg 
      agcgcttgt gaagaaggg aagaaggtg atgtcgagg 
      agaatgggc aagaggaac atcaaggcg caagtgaag 
      aggtttcgc atggagaca aaggcggcc aaaacgctg 
      gcaatcatc gtcggaggt tttatcttc tgttggctt 
      ccgtttttc accatgtat ctagtgcgg gcgttttgt 
      cccgattgc attccaccg ttgctcttc tccatactc 
      ttctggctg gggtattgc aacagtgcc atcaatccg 
      ctcatttat gccctcttc tccaaagac ttcagattc 
      gccttcaag cgcatcatc tgcaagtgc ttctgcaag 
      ggtcgcgat ggaggcggt cgtcgcggt tccgacggt 
      tcgcagctc caaggccgc cagttccga tctccaagc 
      tacaacgtg cagcagcag ggaaattcg cttggagac 
      gacagtgat ccaggcgca gatccgtcg gattcgagg 
      tga
    mRNA sequence
    • >TC011639 RA
      tcagtagca cggcgtcgt ccgaagtgt tcgctcacg 
      tcgcgacgc gcctaaagc gctcgtaat tccagtgat 
      tttcattca aaccttatg ctggcccac actgcacca 
      caaagggtt tcccaatta agaagatga ggtcgtctc 
      gagataaac aagcagcga aggcattat ggaaacgac 
      gaacgatct aacgagatt accgtgtct gtgttgtaa 
      ataatttcc tttcgagga gaatgtctg tgattcggt 
      cgatttaat tttcggtgg tgatggtgc taagacctc 
      gaaaagtgc taatgtgat gcgagaaac actcaacgc 
      atccgcgtg tgcggcgct tctcgatgc tgtcaattg 
      gggggatcc ccaattact tctctcctt atcaatcct 
      agctcttat caatgttat ggtcattgt gggcaactg 
      tttggtcat agctgccgt tttctgctc ctccaaact 
      gcgatcagt gacgaactt gttcatcgt ttctttggc 
      tgttgccga tttactagt cggagttgc agttttgcc 
      cttttcggc gacttggga agtttttaa ggtctggat 
      attcggcga cgtttggtg caacatgtg gctggcttt 
      ggacgtctg gatgtgcac cgcttccat tctcaattt 
      gtgtgccat ttctctgga ccgttatgt ggccgtcac 
      gcgtcccat cacatatcc cagcatcat gtcccattc 
      cagagccaa gctcttaat cgctggctt atgggtgct 
      cagttttgt tatctgttt tccgccgct ggtcgggtg 
      gaaagagac tgaaagacg ttgtccttg gaagtgcga 
      actgactaa cgaggcagg gtatgttgt gtattcagc 
      ccttggtag cttttacat accgatgtt tgtcatgtt 
      gttcttcta ctggaggat ttatcgagc ggctataag 
      aaccaccag ggcaattaa ccaaggatt taggacgac 
      caaaggttc tcgcggttt cggcaaccg tttcgacga 
      gcaacgtct cacgttgcg cattcatcg aggccgtgg 
      ctcttcaga caaacgtcc ccacggctc ccctcacag 
      caacggcag caacagcac caccacaag cagcgtcag 
      ccccagtcc ttccactcg aggcaaaca cgagcgcgt 
      caaaatcag cgtcagtta tccctccag cgaaaacct 
      ttcgcctaa tccaaacct cctagctcc tgcctcacc 
      cacacagtt cgccgttca ttactccgt gaacggaaa 
      ggacaccac cacggcgtt gtacaaacg cgagaacca 
      gctgagagt gcagagact gagctccag gaggagggc 
      ctccaggag gtcaagcag tggcgatag cgaacgcgc 
      accaagtcc gagtccgag cgcttgtga agaagggaa 
      gaaggtgat gtcgaggag aatgggcaa gaggaacat 
      caaggcgca agtgaagag gtttcgcat ggagacaaa 
      ggcggccaa aacgctggc aatcatcgt cggaggttt 
      tatcttctg ttggcttcc gtttttcac catgtatct 
      agtgcgggc gttttgtcc cgattgcat tccaccgtt 
      gctcttctc catactctt ctggctggg gtattgcaa 
      cagtgccat caatccgct catttatgc cctcttctc 
      caaagactt cagattcgc cttcaagcg catcatctg 
      caagtgctt ctgcaaggg tcgcgatgg aggcggtcg 
      tcgcggttc cgacggttc gcagctcca aggccgcca 
      gttccgatc tccaagcta caacgtgca gcagcaggg 
      aaattcgct tggagacga cagtgatcc aggcgcaga 
      tccgtcgga ttcgaggtg acgtaaccg cagacggca 
      cgtttcaca aatgtacct ggacaaaaa ctcggtttt 
      taaagtggc catgtgttt gcacggctg acgaagaaa 
      cctcgccgg agatggcga ccacatcta gacatacta 
      gtctagcat ttatattta ttcgttctt ttttggaga 
      gggaaagat taacgactt aactctttt aggataatt 
      ttaataatg taagacttc atcacgcac aacggttta 
      ttttttaat ttattgatt cgtgcattt gtacattct 
      catcatcgc gaccgataa taaaggctg tgtttattg 
      tagccaaaa atgttaggt aatttttta tgtcatcag 
      atttgatgt ctaatattt aacaacact acctgtcat 
      agagtgtgg aggagaaaa agacaaatt atttaaaca 
      atgttacat atttgttaa aactgtgtt cgacaatgt 
      actctcaat gtaacaagt gtaaataaa acatatacg 
      gaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY