Status
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Gene information
General information
TC012809 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16119002..16123585
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012809
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06529

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012809 RA
      MATFHVLQE GPVNALALN RDNTQVAIG GRNVFKVYT 
      IEEDKFREV CNLRASKHL NLSFSCNDV SWSWTDDHY 
      LATAATNGH VCVWNLTKM GKAMQEQDY QEHKRTVNK 
      VNFHASEPN RLISGSQDG TMRYFDIRA KNAVAIFYS 
      NTESVRDVQ FSPHNPYTF AAVSENGSV QLWDVRRSD 
      KCQQQFAAH SGPIFACDW HPENTWLAT ASRDKTIKV 
      WDLTSKLTL EYTIHTIAS IGHVKWRPQ RRFHIASCA 
      LVVDCSINI WDVRRPYIP YASFNEHKD TPSGVAWKE 
      DPHVFLSSG RDSTLYQHS FDYASRPAK KANPQGVSL 
      NNRGEILYA HKTNVSTLV KAPSVGIIR KTSTTQAPD 
      LFHLASSLM HQFTLSTEN KSESQIFIG LAKNYILSG 
      RSLSEMCEH NSAVANQYG KNQISVVWK IMKTMYGEE 
      YLQNNSNNN NNNREDVIS HNNLPLNMG LDHNNDNDQ 
      RSRGGGDTP AAQFSDETE NEDQVDHIA IYNNGFPTY 
      LNIRTGLPK GDFSFGENE LDMELDGIM TDFQSGYRN 
      FVTPQQDFV LPNEAFHIR HEIRTRSPP PDKFPNNFP 
      VLSDDQNMP FEEQPNNLL TVSCLPKMP LWDPSSIVL 
      DALKHHAML GDVQTAACV LIVLGEQRK CLSDLDEST 
      QEHWLLGYI ELLSRFQLW NIATQIIKL SWIPAVEQL 
      NQQSTRFNT NCSKCSKAL QRIGWLCDR CHSSQYAAC 
      SVCHQVVKG LYAWCQGCS HGGHLLHMK QWFAVNKMC 
      PTGCRHLCE YK
    CDS sequence
    • >TC012809 RA
      atggctaca tttcacgtt ttgcaagaa ggtcctgta 
      aatgcttta gccttaaat agagacaat actcaagtg 
      gcaattggt ggacgcaat gtgttcaaa gtttacaca 
      attgaagaa gacaaattc cgcgaagtg tgcaacttg 
      cgagcatcc aaacacctc aatctgagc ttttcttgc 
      aacgatgtt tcgtggagt tggactgat gaccattat 
      ttggcgacc gccgccacc aacggccat gtctgtgtt 
      tggaatctc acaaaaatg ggcaaagca atgcaagaa 
      caagattat caagaacac aaacgtaca gtaaacaaa 
      gtgaatttt cacgccagt gaaccaaac agactcata 
      tccggctct caagatggc acgatgcgt tattttgat 
      atccgcgcc aaaaacgca gttgctatt ttttacagt 
      aacacagag agtgtccgc gatgtgcag ttcagccct 
      cataatcca tatactttt gcggccgtt tcggaaaac 
      ggaagcgtc caactatgg gatgtaaga agatctgat 
      aaatgccaa cagcaattt gctgcacac agtggaccc 
      atttttgcc tgcgattgg catccggag aacacgtgg 
      ctggccaca gccagtaga gataaaact atcaaagtc 
      tgggatttg acaagtaaa ctgacttta gaatatacc 
      attcatact atagcttca ataggacat gtcaaatgg 
      cgccctcaa cggcggttt catatagct tcatgtgct 
      ttagttgtt gactgtagt ataaatatt tgggatgtg 
      aggaggccc tacattcct tatgcctct ttcaatgaa 
      cacaaggat actccaagt ggagtggct tggaaagaa 
      gatcctcat gttttcctc agcagtggt cgggatagt 
      actttgtat caacattcg ttcgattac gctagccgg 
      ccagcgaaa aaagctaat ccccaaggt gtctcgtta 
      aataatcga ggcgagatt ttgtacgct cacaaaacc 
      aatgttagc accttggtt aaagcacca tcagttggt 
      ataataagg aaaacatca acgactcaa gcacctgat 
      cttttccat ctagcgtct agtttaatg catcagttt 
      accttgtcc accgaaaac aaatccgaa tcacagatt 
      tttattggc ttagccaag aattacata ttgagtgga 
      cggagttta tccgaaatg tgcgaacat aactcggcg 
      gtggcaaac caatacgga aaaaaccaa atttcggtc 
      gtatggaaa ataatgaaa acgatgtat ggcgaagag 
      tatttacaa aacaatagc aataataat aacaataat 
      cgagaagat gtgatttcg cataataac ctacctctg 
      aatatgggt ttggatcat aataatgac aacgatcaa 
      agatcgcgt ggtggtggc gacactcct gccgctcaa 
      ttcagcgac gagaccgaa aacgaagat caagtcgat 
      cacatcgcc atctacaat aacggcttt cccacatat 
      ctaaatatc aggacaggg ttgccgaag ggagatttc 
      tctttcgga gagaacgaa ttggatatg gagttggac 
      gggattatg acggatttt cagagcggt tatcgcaat 
      ttcgtgacg cctcaacag gattttgtt cttccgaac 
      gaagcgttt catattaga cacgaaatc agaactcgg 
      tccccgcca cccgacaag ttcccaaat aattttccc 
      gtgctgagt gacgaccaa aatatgccg tttgaagag 
      caacccaat aatcttctc acagtgtcc tgtttgcca 
      aaaatgccc ttgtgggac ccttcgagt atcgttttg 
      gatgctttg aaacatcac gcaatgttg ggggacgtc 
      caaacggcc gcttgtgtc ttgattgtt ctcggcgag 
      caacgaaaa tgtttgagt gatttagac gaaagcacg 
      caagaacat tggcttttg ggttatatc gaattgtta 
      agtcgattt caactgtgg aatatcgcc acacaaatc 
      atcaagtta tcatggata ccggccgtt gaacaattg 
      aatcaacag tcgactaga ttcaacacg aattgcagc 
      aagtgtagt aaagcgctg caaagaatt ggatggctt 
      tgtgacaga tgtcattcg tcacaatat gcggcttgc 
      agtgtatgt catcaagtt gttaaagga ttatacgct 
      tggtgccaa ggttgttca cacggagga catttatta 
      catatgaaa cagtggttt gctgttaac aaaatgtgt 
      cccacaggg tgtagacat ctgtgcgaa tataagtga
    mRNA sequence
    • >TC012809 RA
      tagactgta aaaaaatca cacaagatc ctcatttct 
      gaccaagat gcgtttacg aacgcttgt tttggtatt 
      tgtattttt gttaacatt ctctccaag ctctgaaat 
      tatcttcga cagtttcat tgctacaaa tgaaatgga 
      aggagcgcg taaaaagaa aaaaggagg tggcagcaa 
      atacactgt accacttct tttcggagc tttaatgat 
      caaatctat tatttttcc gttagcttt taaaactat 
      ggccgtaat gtcaagcat tgcaatact tttaagtac 
      tatgagttt aattatgtc atcaatcgt gggatacat 
      cagaatggg acttaagtc tgcccctgt taaagttgt 
      tcataaaca accaccatc gcccttcaa taattgggc 
      caaagatga tgatggtaa aaattatta cacactcgg 
      agataatta taattatga tttggtggc accaccacc 
      cagataagt aaaaaatat gttatgtaa catttattt 
      aacccaaat aaaatgttt taataacaa tcacttttt 
      cccttctga ttattaaaa tgtactgct tctctgttg 
      tattgtatg gctataact ggtaattgt ttggaagct 
      actgatttc taggaaacg tgaaacgtc agacttgac 
      gtttttagg ttattcctt ttaacctcg cattttttt 
      gttaaatat aaaaaaatg actaaaaac tatttcttt 
      ataataaaa aatgactaa gtaaacgcg tgactgaac 
      gtagtaatt ataaattaa actttatca gtgtcaaat 
      ttataaatc gctggattt tatcaaaga agtgtcaag 
      aatatgtaa acaatggct acatttcac gttttgcaa 
      gaaggtcct gtaaatgct ttagcctta aatagagac 
      aatactcaa gtggcaatt ggtggacgc aatgtgttc 
      aaagtttac acaattgaa gaagacaaa ttccgcgaa 
      gtgtgcaac ttgcgagca tccaaacac ctcaatctg 
      agcttttct tgcaacgat gtttcgtgg agttggact 
      gatgaccat tatttggcg accgccgcc accaacggc 
      catgtctgt gtttggaat ctcacaaaa atgggcaaa 
      gcaatgcaa gaacaagat tatcaagaa cacaaacgt 
      acagtaaac aaagtgaat tttcacgcc agtgaacca 
      aacagactc atatccggc tctcaagat ggcacgatg 
      cgttatttt gatatccgc gccaaaaac gcagttgct 
      attttttac agtaacaca gagagtgtc cgcgatgtg 
      cagttcagc cctcataat ccatatact tttgcggcc 
      gtttcggaa aacggaagc gtccaacta tgggatgta 
      agaagatct gataaatgc caacagcaa tttgctgca 
      cacagtgga cccattttt gcctgcgat tggcatccg 
      gagaacacg tggctggcc acagccagt agagataaa 
      actatcaaa gtctgggat ttgacaagt aaactgact 
      ttagaatat accattcat actatagct tcaatagga 
      catgtcaaa tggcgccct caacggcgg tttcatata 
      gcttcatgt gctttagtt gttgactgt agtataaat 
      atttgggat gtgaggagg ccctacatt ccttatgcc 
      tctttcaat gaacacaag gatactcca agtggagtg 
      gcttggaaa gaagatcct catgttttc ctcagcagt 
      ggtcgggat agtactttg tatcaacat tcgttcgat 
      tacgctagc cggccagcg aaaaaagct aatccccaa 
      ggtgtctcg ttaaataat cgaggcgag attttgtac 
      gctcacaaa accaatgtt agcaccttg gttaaagca 
      ccatcagtt ggtataata aggaaaaca tcaacgact 
      caagcacct gatcttttc catctagcg tctagttta 
      atgcatcag tttaccttg tccaccgaa aacaaatcc 
      gaatcacag atttttatt ggcttagcc aagaattac 
      atattgagt ggacggagt ttatccgaa atgtgcgaa 
      cataactcg gcggtggca aaccaatac ggaaaaaac 
      caaatttcg gtcgtatgg aaaataatg aaaacgatg 
      tatggcgaa gagtattta caaaacaat agcaataat 
      aataacaat aatcgagaa gatgtgatt tcgcataat 
      aacctacct ctgaatatg ggtttggat cataataat 
      gacaacgat caaagatcg cgtggtggt ggcgacact 
      cctgccgct caattcagc gacgagacc gaaaacgaa 
      gatcaagtc gatcacatc gccatctac aataacggc 
      tttcccaca tatctaaat atcaggaca gggttgccg 
      aagggagat ttctctttc ggagagaac gaattggat 
      atggagttg gacgggatt atgacggat tttcagagc 
      ggttatcgc aatttcgtg acgcctcaa caggatttt 
      gttcttccg aacgaagcg tttcatatt agacacgaa 
      atcagaact cggtccccg ccacccgac aagttccca 
      aataatttt cccgtgctg agtgacgac caaaatatg 
      ccgtttgaa gagcaaccc aataatctt ctcacagtg 
      tcctgtttg ccaaaaatg cccttgtgg gacccttcg 
      agtatcgtt ttggatgct ttgaaacat cacgcaatg 
      ttgggggac gtccaaacg gccgcttgt gtcttgatt 
      gttctcggc gagcaacga aaatgtttg agtgattta 
      gacgaaagc acgcaagaa cattggctt ttgggttat 
      atcgaattg ttaagtcga tttcaactg tggaatatc 
      gccacacaa atcatcaag ttatcatgg ataccggcc 
      gttgaacaa ttgaatcaa cagtcgact agattcaac 
      acgaattgc agcaagtgt agtaaagcg ctgcaaaga 
      attggatgg ctttgtgac agatgtcat tcgtcacaa 
      tatgcggct tgcagtgta tgtcatcaa gttgttaaa 
      ggattatac gcttggtgc caaggttgt tcacacgga 
      ggacattta ttacatatg aaacagtgg tttgctgtt 
      aacaaaatg tgtcccaca gggtgtaga catctgtgc 
      gaatataag tgattttat ttatttttc ccacaaaat 
      gaaggtcat gaatgtgcg tgattaaag tgagatgta 
      tcgcagtgt aattataaa taataataa agtttttta 
      tcgtcg
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