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Gene information
General information
TC012803 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15118235..15120989
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012803
    iBeetle screen iB_06527

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages random number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012803 RA
      MSKLPKRVK DKRHIGEHQ KYISRITKT LYYGKFPKT 
      DELSLPVTE LAAELFSEA QKGRSLDRL HCDDAANIS 
      RNACVSPCS LVIAILYLE RLKKTCPEY LERTASSDL 
      FLVSLMVSC KFLYDDGGM SVKQLVQLE KEFLKAIDW 
      EVFVTELTF WKKLSEIET TLARNQGGK RGFYTYTEL 
      ERLLTSVEL RNLIQNILT ISVILITAY TAALLTLLG 
      SVLIVSHIP GACLRPQIE APPSEPLYN ETNRVSPLH 
      VGIFLASIE SFLTPFCNK SETQNVSWD WWSAPTMTW 
      LYETSKFVE VEAPTLGFL DLRRFYKDY FLKLDLEDQ 
      VHKETKVRI QDQLERSWH EEWTDTIGD GFYNRYLHY 
      LQNLKA
    CDS sequence
    • >TC012803 RA
      atgtctaag cttccgaaa cgggtaaag gataagaga 
      cacataggg gagcaccag aagtacatt tcgcggatc 
      accaagacc ctctattac ggtaaattc cccaagaca 
      gatgagctg agtctgcct gtaacagaa ttagccgca 
      gagctgttc tccgaagcc cagaaaggc cggtccttg 
      gacaggctg cattgtgac gatgctgcg aatatttca 
      agaaatgcc tgtgtgtcc ccttgctct ttggttatt 
      gcgattttg tatctagaa cgcttgaaa aagacatgc 
      cctgagtat ctcgaacgc acggcttcg tctgacttg 
      tttttggtt tctttgatg gtttcatgc aaatttctc 
      tatgatgac gggggcatg agcgtcaaa cagttggtt 
      cagctagaa aaggagttc ctcaaagct attgactgg 
      gaagtcttc gttactgag ctgacattt tggaagaaa 
      ctgtccgaa atcgaaacg accttggcc cgcaaccag 
      ggtggaaaa cgcggcttt tacacctac accgaacta 
      gagcgtctt ttaacaagc gtcgaattg cgaaacctc 
      atccaaaac attttgacc atttctgtg atcctcatt 
      acggcttac acggccgcc ttattgact ttattgggc 
      tctgtcttg attgttagc cacattccg ggggcttgt 
      cttcgcccg caaatcgaa gcgccgccc tcggagccc 
      ctctacaac gaaacgaac cgtgtgagc cccctccat 
      gtcggaatt ttcctcgca agtattgag agttttctg 
      acaccgttt tgcaacaaa agtgagact caaaacgta 
      tcctgggac tggtggagt gcccccacg atgacatgg 
      ctctacgaa acgtcgaaa tttgtcgaa gttgaggct 
      ccgactttg ggttttttg gacttgaga cgattctat 
      aaggattat tttttgaaa ttggatttg gaggatcaa 
      gtgcataag gagacgaaa gttaggatt caagaccag 
      ttggagagg tcgtggcac gaagaatgg acggatacg 
      ataggtgat gggttttat aacaggtac ctgcattac 
      ctacaaaat ctcaaagcc tga
    mRNA sequence
    • >TC012803 RA
      tttgtgggg ttagattat aaattagtt gtgtgcttg 
      ggtctgtga tggccacca agggcgaag ttgcaagtt 
      tggttggtt tatttagag tttgaaaaa aattgaggt 
      tagggggcc acgcgcatg tccggtgac attgccaag 
      gtccatgat taaaaaaac actgattgc aaatctgtg 
      attaattat acgcaattg cgaatcata cgacacgtt 
      ttggggctg ttccaagtg ggtaagaca atgtctaag 
      cttccgaaa cgggtaaag gataagaga cacataggg 
      gagcaccag aagtacatt tcgcggatc accaagacc 
      ctctattac ggtaaattc cccaagaca gatgagctg 
      agtctgcct gtaacagaa ttagccgca gagctgttc 
      tccgaagcc cagaaaggc cggtccttg gacaggctg 
      cattgtgac gatgctgcg aatatttca agaaatgcc 
      tgtgtgtcc ccttgctct ttggttatt gcgattttg 
      tatctagaa cgcttgaaa aagacatgc cctgagtat 
      ctcgaacgc acggcttcg tctgacttg tttttggtt 
      tctttgatg gtttcatgc aaatttctc tatgatgac 
      gggggcatg agcgtcaaa cagttggtt cagctagaa 
      aaggagttc ctcaaagct attgactgg gaagtcttc 
      gttactgag ctgacattt tggaagaaa ctgtccgaa 
      atcgaaacg accttggcc cgcaaccag ggtggaaaa 
      cgcggcttt tacacctac accgaacta gagcgtctt 
      ttaacaagc gtcgaattg cgaaacctc atccaaaac 
      attttgacc atttctgtg atcctcatt acggcttac 
      acggccgcc ttattgact ttattgggc tctgtcttg 
      attgttagc cacattccg ggggcttgt cttcgcccg 
      caaatcgaa gcgccgccc tcggagccc ctctacaac 
      gaaacgaac cgtgtgagc cccctccat gtcggaatt 
      ttcctcgca agtattgag agttttctg acaccgttt 
      tgcaacaaa agtgagact caaaacgta tcctgggac 
      tggtggagt gcccccacg atgacatgg ctctacgaa 
      acgtcgaaa tttgtcgaa gttgaggct ccgactttg 
      ggttttttg gacttgaga cgattctat aaggattat 
      tttttgaaa ttggatttg gaggatcaa gtgcataag 
      gagacgaaa gttaggatt caagaccag ttggagagg 
      tcgtggcac gaagaatgg acggatacg ataggtgat 
      gggttttat aacaggtac ctgcattac ctacaaaat 
      ctcaaagcc tgatttgat tggtcaata aaaccaaac 
      atttgacac gattgttta ttcaccagc ctactcaaa 
      ctacaatta atgggtctt tatcgacac ttgaaacaa 
      taatattga ctgaatttt gcaacaaat cactcaatt 
      gtttctgga aacgcccca aaaagcccc gttcggaat 
      tactatgtt cgcagagaa tcacatgaa ccccgtttt 
      gcgtagcat ccaggactt catggtggg acatttccc 
      ccgttaggt acaaatcgc tcgtgagcc cctttaaaa 
      ccgaagccc ccgaaccgg cacacagcc ccacggttt 
      caatcctcg catctgaaa atcactttc gtttcactc 
      aatttcaag ggcgtggct tacctaaac ctttgttcc 
      tagccaatg cgagtcgca gatgcccca ccccgacat 
      gttttttga catttgcga ccgcggttt ccacagtta 
      tgggcgtgt ctagtgtga ggatgcgcc ccgcccctt 
      gtctcggat tttccaatg gtttgattg gcgcaatgt 
      ttttaaaag cgaacgctg aggctagcc aatcattaa 
      cgccccctt cgacacaat cccagctcg tatggggcg 
      agtaaaccg caatttcat gtctaatac attggatta 
      cgattcgtt ctttccaag tcctgtgac aaaacgatt 
      agttttgat tggctgtgt aatttgggg cgtacttgt 
      cagagactg ttttaaggc cttgaaaga tattagggt 
      gataggaga taatgagcc ccgcttgga agtttttag 
      cttcattga ccacatcct cagtgaggt cgtttgtta 
      atagaattg ttttgacct gtttgtcgg tcattgggt 
      ccaccagga gccccacat tgtcccagc tctcggcaa 
      gggtcaagg gggcgtagg attgtaaag ttttgacga 
      tttctttga ccgacattt gggggcccg tgctgtagt 
      aatttttaa tccgactta gggccgcca accgtctta 
      ttaacatct gtggaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY