Status
Please wait ...
Gene information
General information
TC011691 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15030600..15034117
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011691
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06525

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011691 RA
      MSATNQSLK KTLVIFLLS FHSIRTKFL DDICGHING 
      QRLFLDHGE TGTLQANYS NNIFDRFYK NKPEKRCTV 
      ELVSCPSCV IKITFTFLN LTCGRSFDT CACDHVWIY 
      EPTFEQSSQ QFCRSSNTS MVYQSQTRR AMVVFLYSK 
      RYENAFSLD YVSNRNKVV LNGNGTQML QSPFFPNLY 
      PSDLTMEYT IGCKSENDT CKISLVFTD FLIAPPSIL 
      DFFDSSGDR IYVTTGNIF RPPVITSGS ALVIRFYAN 
      GATSPGFKA YYSFINDVT NIDCGGQVN NLGGGITMM 
      DMVQDGLKS FDCIWLVKP PETYLHMKT HLYVKVVNF 
      SGFAGSTEL TIRQGLTSN GPVVENLKL SLPLFYTQK 
      KREHIVPIS QGFYIRLKG FFSPQSRLA IVYAAFNYK 
      DCLSGSDFL CHNKRCVSS LLNCDGFDH CGDNSDESS 
      ISCSKDPND RRQWSQTPH FLFPKVSTL SDFATSTLV 
      FLLCSFGLM GFIFAMVVL LYRINIKSR HQRQIQDHI 
      ETINAILEE GVVEIEEEI IIPDDPPDY EAPPDYDQV 
      VKCFVSKKK RCSKCRVKI PESPPPAYN QGPSTSRVL 
      HFFANRKCY RGRRVRFLS DSECDQKKS KGFLRKSFT 
      ADDMTLFI
    CDS sequence
    • >TC011691 RA
      atgagcgcc acaaaccaa agtttaaaa aagacactc 
      gtaattttc ctcctctcg tttcactca atccgcacg 
      aaatttctc gacgacatt tgtggccac atcaacggc 
      cagcggctt ttcctcgac catggcgaa acgggcact 
      ttgcaagcc aactactcc aacaatatt ttcgaccgt 
      ttttacaaa aataaaccc gaaaaacgg tgcactgtc 
      gagcttgtt tcatgccca tcatgcgtc ataaaaatc 
      accttcact tttttgaat ttgacttgt ggccgctcg 
      ttcgatact tgcgcctgt gaccatgtc tggatctac 
      gaacccacg ttcgaacag tcgtcccag cagttctgc 
      aggtcttca aacacttcc atggtgtac caaagccag 
      acgaggcga gccatggtg gtgtttttg tactcaaag 
      aggtacgaa aatgcgttc agtttggac tacgtgtcg 
      aaccggaac aaagtggta ctgaacggg aatgggaca 
      cagatgtta caaagccca tttttcccc aacttgtac 
      ccatccgac ctgaccatg gaatacacc attgggtgc 
      aagtccgaa aacgacact tgcaagatt agtctcgtt 
      ttcaccgac tttctcata gcgccacct tcgatcctt 
      gattttttt gactcatca ggggaccgt atttatgta 
      acaaccgga aatattttt cgcccacct gttataact 
      tccgggtct gctcttgtg ataagattt tacgcaaac 
      ggggccact agtcccggt ttcaaggcc tactattct 
      tttatcaac gatgtcacg aatatagat tgcgggggg 
      caagttaat aatcttggg ggcgggatt accatgatg 
      gacatggta caagatggg ttgaagtcg tttgattgc 
      atttggttg gtcaaacca ccggagacg tacctccac 
      atgaagacg catttgtac gtcaaggtg gtcaatttt 
      tcgggtttt gcgggaagt accgaactg acgattcgg 
      caaggtttg acctcgaac ggcccagtg gtcgagaat 
      cttaaactg tctttacct ttattctac acccaaaaa 
      aagcgcgaa catattgtg ccaatatct caagggttt 
      tacattcgg cttaagggt tttttcagc cctcagtcc 
      cgtctagct atagtttac gctgctttc aattacaaa 
      gattgtctc tcgggttct gatttcctc tgtcacaat 
      aaaagatgc gtctcttct ctgcttaac tgtgacggt 
      tttgaccac tgtggtgac aacagtgac gaatcctcc 
      atcagttgc tccaaagac cccaatgac cgacgccaa 
      tggtcccaa accccccat ttcctcttc cccaaagtg 
      tccactttg tccgacttt gccacctca actctagtc 
      tttctctta tgtagtttt ggcttgatg ggttttatt 
      ttcgcaatg gtggtactc ctctaccgc ataaatatc 
      aaatcgcgt caccagaga caaatccaa gaccatatc 
      gagacaatt aatgcaatt ctggaggaa ggggttgtt 
      gaaattgag gaggagatt ataatcccg gacgatcct 
      ccggattat gaggcaccg cctgattac gaccaggtg 
      gttaagtgt tttgtttcg aagaagaag aggtgttcc 
      aagtgtcgg gttaaaatc ccggagtcg ccaccaccg 
      gcctataat caagggcct tcgacgtca cgagttttg 
      cactttttt gcaaaccga aagtgttat agaggcagg 
      agagtcagg tttttgtcc gactctgag tgcgatcaa 
      aaaaagtcg aagggtttt ttgaggaag agttttaca 
      gctgacgat atgactctg tttatttaa
    mRNA sequence
    • >TC011691 RA
      tttttttgt atttgttag taaagacat gagcgccac 
      aaaccaaag tttaaaaaa gacactcgt aattttcct 
      cctctcgtt tcactcaat ccgcacgaa atttctcga 
      cgacatttg tggccacat caacggcca gcggctttt 
      cctcgacca tggcgaaac gggcacttt gcaagccaa 
      ctactccaa caatatttt cgaccgttt ttacaaaaa 
      taaacccga aaaacggtg cactgtcga gcttgtttc 
      atgcccatc atgcgtcat aaaaatcac cttcacttt 
      tttgaattt gacttgtgg ccgctcgtt cgatacttg 
      cgcctgtga ccatgtctg gatctacga acccacgtt 
      cgaacagtc gtcccagca gttctgcag gtcttcaaa 
      cacttccat ggtgtacca aagccagac gaggcgagc 
      catggtggt gtttttgta ctcaaagag gtacgaaaa 
      tgcgttcag tttggacta cgtgtcgaa ccggaacaa 
      agtggtact gaacgggaa tgggacaca gatgttaca 
      aagcccatt tttccccaa cttgtaccc atccgacct 
      gaccatgga atacaccat tgggtgcaa gtccgaaaa 
      cgacacttg caagattag tctcgtttt caccgactt 
      tctcatagc gccaccttc gatccttga tttttttga 
      ctcatcagg ggaccgtat ttatgtaac aaccggaaa 
      tatttttcg cccacctgt tataacttc cgggtctgc 
      tcttgtgat aagatttta cgcaaacgg ggccactag 
      tcccggttt caaggccta ctattcttt tatcaacga 
      tgtcacgaa tatagattg cggggggca agttaataa 
      tcttggggg cgggattac catgatgga catggtaca 
      agatgggtt gaagtcgtt tgattgcat ttggttggt 
      caaaccacc ggagacgta cctccacat gaagacgca 
      tttgtacgt caaggtggt caatttttc gggttttgc 
      gggaagtac cgaactgac gattcggca aggtttgac 
      ctcgaacgg cccagtggt cgagaatct taaactgtc 
      tttaccttt attctacac ccaaaaaaa gcgcgaaca 
      tattgtgcc aatatctca agggtttta cattcggct 
      taagggttt tttcagccc tcagtcccg tctagctat 
      agtttacgc tgctttcaa ttacaaaga ttgtctctc 
      gggttctga tttcctctg tcacaataa aagatgcgt 
      ctcttctct gcttaactg tgacggttt tgaccactg 
      tggtgacaa cagtgacga atcctccat cagttgctc 
      caaagaccc caatgaccg acgccaatg gtcccaaac 
      cccccattt cctcttccc caaagtgtc cactttgtc 
      cgactttgc cacctcaac tctagtctt tctcttatg 
      tagttttgg cttgatggg ttttatttt cgcaatggt 
      ggtactcct ctaccgcat aaatatcaa atcgcgtca 
      ccagagaca aatccaaga ccatatcga gacaattaa 
      tgcaattct ggaggaagg ggttgttga aattgagga 
      ggagattat aatcccgga cgatcctcc ggattatga 
      ggcaccgcc tgattacga ccaggtggt taagtgttt 
      tgtttcgaa gaagaagag gtgttccaa gtgtcgggt 
      taaaatccc ggagtcgcc accaccggc ctataatca 
      agggccttc gacgtcacg agttttgca cttttttgc 
      aaaccgaaa gtgttatag aggcaggag agtcaggtt 
      tttgtccga ctctgagtg cgatcaaaa aaagtcgaa 
      gggtttttt gaggaagag ttttacagc tgacgatat 
      gactctgtt tatttaatt ttgaagcaa atagtatat 
      gattatatt ctctaataa aatgtgcgt tatgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY