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Gene information
General information
TC011692 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14998560..15004387
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011692
    iBeetle screen iB_06524

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011692 RA
      MTAFIVARS LPVLNRAIK QFLSNDLTH QARVIQCVI 
      PSLTQSRFY AAEKKVFDR SKPHCNVGT IGHVDHGKT 
      TLTAAITKV LADQKLAEA KKYQDIDNA PEEKARGIT 
      INVAHIEYQ TENRHYGHT DCPGHADYI KNMITGAAQ 
      MDGGILVVA ATDGVMPQT REHLLLAKQ IGVDHLVVF 
      INKVDAADK EMVELVEME IRELMTQMG FDGDNVPIV 
      AGSALCALE GKNPEIGSE AVLKLLKEV DNYIPTPTR 
      ELDKPFLLP VEHVYSIPG RGTVVTGRL ERGVVKKGN 
      DCEFVGYNK VLKSTVTGV EMFHQILEE AQAGDQVGA 
      LVRGVKRDD IKRGMVMAK PGTVKSYDH IESQVYILS 
      KDEGGRTKP FTSYIQLQM FCRTWDCAI QVIVPDKEM 
      VMPGEDSKL ILKLLRPMV LEQGQRFTL RDGSQTLGT 
      GVVTKVLPA LKENERIQL LEGKKAREK RQAQK
    CDS sequence
    • >TC011692 RA
      atgaccgct tttatcgtc gcacgctcg ctcccagtg 
      ttaaatcgt gcgattaaa cagtttctt tcgaatgat 
      ttgacgcac caagcgcgc gtgatccaa tgcgttatc 
      ccgagcttg acccagagt cggttttac gccgctgag 
      aaaaaagtt ttcgaccgg tcgaagccc cactgtaat 
      gtgggcacg atagggcac gtggaccac ggcaaaacc 
      actctgact gcggctata actaaagtc ctagccgac 
      caaaagctc gctgaagcg aaaaagtac caggatatt 
      gacaatgcc cccgaggag aaggcccga ggcattacg 
      ataaatgtg gctcatatt gagtatcag actgagaac 
      aggcattac gggcatacg gactgcccc gggcatgcc 
      gattatatt aaaaatatg atcacgggg gctgcgcag 
      atggacggg ggcattctc gtggtggcg gccaccgat 
      ggggtcatg ccccaaact agggaacac ttgctcctg 
      gccaagcaa ataggggtg gaccacttg gtcgtgttt 
      atcaataaa gtggacgcg gctgataag gagatggtc 
      gagttggtg gagatggag attcgggaa ttgatgacc 
      cagatgggc tttgacggg gataatgtg cccattgtt 
      gctgggtcg gctctgtgt gcccttgaa gggaaaaat 
      cccgagatt gggtccgag gcggttctt aaacttttg 
      aaagaagtt gataattac attcccact ccgacacgg 
      gagttggat aagccgttt ttgctgccc gttgaacat 
      gtctattct attcctgga cgggggact gttgtgacg 
      gggcgtctg gaacgaggg gttgtgaaa aaagggaat 
      gattgcgaa tttgtgggg tacaacaag gttttgaag 
      agtacagtg acgggggtg gagatgttt caccagatt 
      ttggaggag gcgcaagcc ggggatcaa gtgggggct 
      ttggttagg ggagtcaaa agggatgat attaagaga 
      ggtatggta atggccaag cccggcact gtgaaaagt 
      tacgaccac attgagtct caagtctat atcttgagc 
      aaggacgag ggtggcagg actaagccc ttcacttct 
      tacatccaa ttacagatg ttttgtagg acttgggat 
      tgcgcaatt caggtcatt gtgcccgat aaagaaatg 
      gtgatgcca ggagaggac tccaagttg attttgaaa 
      ttattaaga ccgatggta ctagaacaa ggacaaagg 
      tttactttg agggacggt agccagact cttggtact 
      ggggttgta acaaaagtc ttgcccgct ctaaaagaa 
      aatgaaaga atacagttg ttggaaggg aagaaagcc 
      agagagaag aggcaggca cagaaataa
    mRNA sequence
    • >TC011692 RA
      ggggccgcg ccacccaaa aaccgcccc aaaatgatt 
      gcgcttacc atgagaaag aggctgaaa atgagcctg 
      cagcggtcc atttcgggc actgaaata aagcccgca 
      cacggcact cgaattact cctgcagag cagcaggtg 
      ctagttctt cggttggtg ttgaactgg agaagcgcg 
      cgcgatgaa tggtgcaga agaagacgt tacagcagg 
      caggcagga ccccacagt gttttccct ctattgtag 
      tcaggatgt tcttcatcc ttattggac gtctcgact 
      ccacttttc gtgactatt ttttgtccc caaatccgc 
      cattttagt gcccgtgtg ttgaataat gcccatgcg 
      gctaaaact cgcctaatt acgtataaa aacgcagtt 
      tttgtgctc acaattgtc gttttatcg aactttcgg 
      taaaaatga ccgctttta tcgtcgcac gctcgctcc 
      cagtgttaa atcgtgcga ttaaacagt ttctttcga 
      atgatttga cgcaccaag cgcgcgtga tccaatgcg 
      ttatcccga gcttgaccc agagtcggt tttacgccg 
      ctgagaaaa aagttttcg accggtcga agccccact 
      gtaatgtgg gcacgatag ggcacgtgg accacggca 
      aaaccactc tgactgcgg ctataacta aagtcctag 
      ccgaccaaa agctcgctg aagcgaaaa agtaccagg 
      atattgaca atgcccccg aggagaagg cccgaggca 
      ttacgataa atgtggctc atattgagt atcagactg 
      agaacaggc attacgggc atacggact gccccgggc 
      atgccgatt atattaaaa atatgatca cgggggctg 
      cgcagatgg acgggggca ttctcgtgg tggcggcca 
      ccgatgggg tcatgcccc aaactaggg aacacttgc 
      tcctggcca agcaaatag gggtggacc acttggtcg 
      tgtttatca ataaagtgg acgcggctg ataaggaga 
      tggtcgagt tggtggaga tggagattc gggaattga 
      tgacccaga tgggctttg acggggata atgtgccca 
      ttgttgctg ggtcggctc tgtgtgccc ttgaaggga 
      aaaatcccg agattgggt ccgaggcgg ttcttaaac 
      ttttgaaag aagttgata attacattc ccactccga 
      cacgggagt tggataagc cgtttttgc tgcccgttg 
      aacatgtct attctattc ctggacggg ggactgttg 
      tgacggggc gtctggaac gaggggttg tgaaaaaag 
      ggaatgatt gcgaatttg tggggtaca acaaggttt 
      tgaagagta cagtgacgg gggtggaga tgtttcacc 
      agattttgg aggaggcgc aagccgggg atcaagtgg 
      gggctttgg ttaggggag tcaaaaggg atgatatta 
      agagaggta tggtaatgg ccaagcccg gcactgtga 
      aaagttacg accacattg agtctcaag tctatatct 
      tgagcaagg acgagggtg gcaggacta agcccttca 
      cttcttaca tccaattac agatgtttt gtaggactt 
      gggattgcg caattcagg tcattgtgc ccgataaag 
      aaatggtga tgccaggag aggactcca agttgattt 
      tgaaattat taagaccga tggtactag aacaaggac 
      aaaggttta ctttgaggg acggtagcc agactcttg 
      gtactgggg ttgtaacaa aagtcttgc ccgctctaa 
      aagaaaatg aaagaatac agttgttgg aagggaaga 
      aagccagag agaagaggc aggcacaga aataaacaa 
      gtagctttt aagttatta ccttgtact ttatgttaa 
      tgaaaatag tgtactatg aagatgttt ttaccgtgt 
      aattaataa aaattatca aaagct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY