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Gene information
General information
TC034453
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012780
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14638976..14655672
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034453
    iBeetle screen iB_06523

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034453 RA
      MTHGSLGYR KLGLDLVPR QGAQMIDPD SMSVVELYH 
      FHVSSAENS QGLSARGTI KKKEPKKTL THHLYFCMR 
      DFGHHIGED TEIYFSLYD ASKQKYISE RFLVKISKE 
      GFSNYVEKL HSNCTVFTD LGNSDLSRD IYLVAHVMR 
      IGKMIYSDN KKTEKNAVL LQQVFKRPH GVAVHNLGE 
      YLMPKEGDS EEKELNLKV YQGEEKDFH QLHEFIIRQ 
      SGKYNALAC APNYGILTS VKMLHGGLN HIKEENAML 
      FKNVSLTSK LGFPDVIMP GDVRNDLYL ILEKGEFER 
      GGKSTGKNI EVSIVVLDS EKNVIRNCL WGASGQDGA 
      SEYNSMIIY HHNSPAWAE NVRLTLPID KFAGAHVRL 
      EYRHCSTRE KNDKKLFGF SFIRLMDKD GAAVQDGQH 
      ELYIYKCED PQKLENCGY LSLPAFSKD LEGNHDSVG 
      QFSRSHKEV VFVRTLLCS TKLTQNVDL LALLRWKSH 
      PEKIQDSLQ RVLRLGDEE LIKFLQDVL DALFALFST 
      EDGNSTAYS GLVFHVLVS IFNLLDGSK YQHFKPVLD 
      AYIKDHFAA ALVYKGLLT SVQHCADWV LSFDKQEPI 
      QKCYKSLEY IFKLIIQSR LLFSRATCG QYEDSFRRD 
      LYSVFSSLN KMLTVNDPH IINTQVALL LSISAVYEQ 
      LIEVLPTIE VTKLAATLL DAIPKDLPV LLVQAKLSC 
      IKNLVTSKL FQDDESRNI LLANACKHL RAQLHRREE 
      LKLCTDILG EILGFLFKQ RKQFEGQGK INNCIHHDV 
      ETLCINILD ILVQAVLTT IDFKDVKDG KILGCLVAC 
      LIGILQLLD EFHYKRLWE VLMGPGLDR KPLKDFLLR 
      VFLVFRNLV RLEVFPPDW LVMKMVVNN VMLKSLQEL 
      AQPLAFKFL DSRSGYFDK ELWTDYFNL AVDYLTQKP 
      LQLEHFSEV KREKIIEKY GDMRVLMGF QILSMWSQL 
      GECKLHFIP SMVGPFLKV TLVPEIELR KATLHIFFD 
      MMECEQRAR GNFRQVESE LIDKLDILI SENKGDDEY 
      RRLFNTILL DRVQSEDLA WKESGAAFI SSITRLLER 
      LLDYRSVIE GDENRDKRM SCTFNLLNF YKNEFNRKE 
      MYLRYIYKL HDLHLSAEN YTEAAFTMK LYADQLTWS 
      STTLVADNH FPNFTECQV KEKLYRQII NYFDKGKCW 
      EKGIPLLKE LATLYEVQF FDYKALSDI LKYQAKFYD 
      NILTQLRPE PEYFRVGFY GLSFPLFVR NKQFVYRGL 
      EYERIGAFT QRLQTEFPS AQILMKNTP PDDSIVNSE 
      GQFIQICNV KPIPEPNPI TLATDIPEK ISRFYHYND 
      VKRFQCDRP VHKGIIDKE NEIKTLWIE RMTLEIENP 
      LPGILRWFE VINRHTDEI PPVKYACET MQSVELELR 
      QLITTYTIE PKRNLNPLT MRLQGIIDA NVQGGISKY 
      QQAFFTQEF AKLCPEHMT YVYTLKNLI LDAMQVIEE 
      GLELHGRLA PAGVVPLHH RLLERFAQL KESLGALSK 
      IKRQIPDSI VNTPLPPLP IDKRLMVVE NGHHRPGNY 
      DYLSEHRTL ETDDIYTKP MENERPLRE NLTLPMATT 
      TAPPIPQRE LRPRSQGFT NNFSEAQTP TRLDYNTSS 
      LPLMKTRNS DSYDIPLPP KPTHSRERS LTKPPSPRL 
      LRHASMTPT DGTSPLRNS WSDSGVEEA PPLPPRSHT 
      PDKRLGFSE PPPNIPKRG QKKSLVGAA ETECELTPD 
      SLRDSGISM SENSNLNNL NNTCYEDFD LKGHQQEMN 
      ISSSPFEGS PKLENPPPI PPKASLSSS LEAAREAEA 
      ATPDSDKEK SPS
    CDS sequence
    • >TC034453 RA
      atgactcat ggttcgctt ggttacagg aaattggga 
      ctggatctg gtgcccaga caaggggcg caaatgatc 
      gatccggac tcgatgtct gtggtcgaa ttgtaccat 
      tttcatgtc tcgagtgcc gagaactcg caagggctg 
      tcggcccgc ggcaccatc aagaaaaaa gaaccgaaa 
      aaaacgcta acccaccac ctctacttc tgcatgcgc 
      gacttcggc caccacatc ggcgaagac accgaaatc 
      tacttctct ctgtacgac gcctccaag cagaaatac 
      atcagcgaa cgtttccta gtcaaaata tccaaagag 
      ggtttctcg aactacgtg gagaagttg cactccaac 
      tgcaccgtc ttcacggac ttgggcaac tccgacctg 
      agccgggac atctacctg gtggcccac gtgatgcga 
      atcggcaag atgatctat tccgacaac aagaagact 
      gaaaagaac gccgtcctg ctgcagcag gtgttcaag 
      cggccgcac ggggtcgcc gtgcacaac ctgggggag 
      tacctcatg cccaaggag ggcgactcc gaggagaag 
      gagctcaac ctgaaggtg taccaaggc gaggagaag 
      gacttccac cagctgcac gagtttata ataaggcag 
      tcggggaag tacaacgcc ttggcttgc gcccccaac 
      tatgggatc ctcacctcg gtgaagatg ctccacggg 
      gggctgaac cacatcaag gaggaaaac gcgatgctg 
      ttcaaaaac gtgagcttg acgagcaag ttggggttt 
      ccggacgtg attatgcca ggggatgtc aggaacgac 
      ttgtatctc attctggag aagggggag tttgaacgc 
      gggggtaaa tccacgggg aaaaatatc gaagtgagc 
      attgtggtc ctggacagt gagaaaaat gtcatcagg 
      aattgtctg tggggggct tcggggcaa gatggggct 
      tcggaatac aattccatg attatttat catcacaat 
      tcgccagct tgggccgaa aatgtgagg ctgactctg 
      ccgattgat aagttcgcg ggggcgcat gtgaggctg 
      gagtacagg cattgctca acacgcgaa aaaaacgac 
      aagaaactc ttcggcttc tccttcatc cgtctgatg 
      gataaagac ggagccgcc gtccaagac ggccaacac 
      gaactctac atttacaaa tgtgaagac ccccaaaaa 
      ctcgaaaac tgcggctat ttgtctctc ccagccttc 
      agcaaagac ctcgaaggc aaccacgac agtgtcggc 
      caattttct cgaagccac aaagaggtg gttttcgtt 
      agaaccctc ctatgttcc accaaactg acccaaaac 
      gtcgatttg ctagcactg ttacggtgg aaatcccac 
      ccggaaaaa atccaggac tccttgcag cgcgttttg 
      cgactcgga gacgaggaa cttatcaag ttcctccag 
      gatgttctg gatgctctg tttgccctt ttctcgacc 
      gaagatggc aacagtacc gcctacagc ggcctggtg 
      ttccacgtt ttggtctca atttttaac ctccttgat 
      ggttcaaag taccaacac tttaagcca gtcctggat 
      gcgtacatt aaggaccat ttcgccgct gctttggtc 
      tacaaaggc ctgctaaca agtgtgcag cactgtgcc 
      gattgggtc ttatctttc gacaaacaa gaaccgatc 
      caaaagtgt tacaaaagt cttgagtac attttcaaa 
      ctcataatc cagagccgg ctgttattc tcacgcgcc 
      acgtgtggc caatacgag gacagcttc cgacgggac 
      ttatacagc gttttttct tcactcaac aaaatgttg 
      acagtgaat gatccccac attatcaac acacaggtg 
      gcactttta ctctcaatt agcgcagtc tacgagcaa 
      ctgatcgaa gttttgccc acgattgaa gtaacaaag 
      ttggccgcg accctcctg gacgccatc ccgaaggac 
      ttaccggtg ctcctagtc caggccaaa ctatcctgc 
      atcaaaaac ctggtcaca agcaaattg tttcaagac 
      gacgagtcc agaaacatt ctcctggca aatgcttgc 
      aagcacttg cgtgcgcaa ctccaccgt cgggaagaa 
      ctcaaactt tgcaccgat attttgggc gaaatcctg 
      ggatttttg ttcaaacaa aggaaacag tttgaagga 
      caggggaaa atcaacaat tgcatccac cacgatgtt 
      gagacgctt tgtatcaac attttggac attttggtc 
      caggctgtg ctaacaacc attgatttt aaggacgtc 
      aaggacggg aaaattctg ggctgtttg gtggcgtgt 
      ttgattggg attttgcaa cttttggac gagtttcac 
      tacaaacgg ctgtgggaa gtcctaatg gggccgggc 
      ctggaccgg aagcccctc aaagacttc ctcttgcgt 
      gttttccta gttttccga aatttggtg cgtttggag 
      gttttcccc cctgattgg ttggtcatg aaaatggtg 
      gttaacaac gttatgcta aaatcgcta caagagtta 
      gcacaacct ttagccttc aagtttttg gactcgcgg 
      agcggttac ttcgacaaa gagctttgg accgactat 
      ttcaacctc gctgtggac tacttgacc cagaagccg 
      ctccaactg gagcacttc tccgaagtg aagcgcgag 
      aaaattatt gagaagtac ggcgatatg agggttttg 
      atgggcttc cagatcttg tcaatgtgg tcgcagttg 
      ggcgaatgc aaactacat tttattccg tcgatggtt 
      ggaccgttc cttaaagta acactagtt cctgagatt 
      gaattacga aaggccaca ctgcacatt tttttcgac 
      atgatggag tgcgagcaa agggcgcgc gggaatttc 
      agacaggta gagagtgag ctgatcgac aagctggat 
      attctcatc tcggaaaat aagggcgat gatgagtac 
      cgcaggctt ttcaatact atcctgcta gaccgcgtc 
      caatcggag gatttggcg tggaaggag agcggtgca 
      gctttcata agctccatc acacgtctc cttgaacgc 
      cttctcgac taccgcagc gtcatagaa ggcgacgaa 
      aaccgcgac aagcgaatg tcttgcact ttcaatctt 
      ctgaatttc tacaaaaac gagttcaat cgtaaagaa 
      atgtatctc cgctacatt tataagctc cacgatctt 
      catttatcg gcggagaac tacacagag gcggcgttt 
      acgatgaaa ctgtacgct gatcagctg acttggagt 
      agtaccaca ctggtggcc gataatcat tttccgaat 
      tttaccgaa tgccaggtt aaggagaag ttgtacaga 
      cagattatt aattatttc gataagggg aagtgttgg 
      gagaagggg attcccctg ttgaaggag ctggcaaca 
      ctgtacgaa gtccagttt ttcgactac aaagctctg 
      agtgatatt cttaaatac caagcgaaa ttttacgat 
      aatattttg acgcagctc cgtccagaa ccagaatat 
      tttagggtg ggcttctac gggcttagc tttccgttg 
      tttgtgagg aataaacaa tttgtatat aggggatta 
      gaatacgaa cgcatcggt gcctttact caaagactt 
      caaacggag tttccctca gctcagata cttatgaag 
      aacacgcca cccgacgac tcgatcgtc aactcagag 
      gggcaattt atacaaatt tgcaacgtg aaaccaatc 
      cctgaaccg aacccgatt actctagcg accgacatt 
      cccgagaaa atctcccgg ttttaccac tacaacgac 
      gtgaaaagg ttccagtgc gaccgtcct gtacataaa 
      ggaattata gataaggag aatgagatt aagactttg 
      tggatcgaa cgcatgact ctggagatc gagaacccc 
      cttccgggg attttgcgc tggtttgag gtcatcaat 
      cgccacact gacgaaata ccgcctgtt aagtacgcc 
      tgcgagacg atgcagtcg gtcgagctc gaattgagg 
      cagttaata acgacttat acgatcgaa ccgaagcga 
      aatttgaac ccgctgacc atgaggctc caagggatt 
      attgatgcg aacgtccag ggcgggatt agcaaatac 
      cagcaggcg tttttcacg caagaattc gcaaaactt 
      tgccctgaa cacatgacc tatgtttac accctcaaa 
      aacctgatt ctggacgct atgcaagtg attgaggaa 
      gggttagag ctacacggg aggttagcg ccggccggt 
      gtagtcccc ctccaccac cggctcctg gagcgtttt 
      gcccaactg aaggaaagt cttggtgct ttgagcaaa 
      atcaaacga caaatcccc gacagtatt gtaaacacc 
      cccctcccc ccgttgccg atcgacaaa agactgatg 
      gtggtggaa aacggccac caccgaccc ggaaactac 
      gactactta tccgaacac cgaacgctg gaaaccgac 
      gacatctac accaaaccc atggaaaac gaacggccg 
      ctccgcgaa aacctaaca ctgccgatg gccacgacg 
      acagcgcca cctatacct caacgcgaa ctcagaccc 
      agatctcag ggattcacc aataacttc agcgaggcg 
      cagacgccg accagactg gactacaac acgtccagc 
      ttgcccttg atgaagacg cgcaactcg gactcgtac 
      gacattcca ctgcctccg aagccgacg cattcgagg 
      gagcggtcg ctcacgaag ccgccgtcg ccgcggctg 
      ctgaggcac gcctccatg acgccgacg gacgggacg 
      tcgcctttg aggaactcc tggtccgac tcgggggtg 
      gaggaggcg cccccgctg ccgccgagg tcgcacacc 
      cccgacaag cgtctgggg ttcagcgag ccgccgccc 
      aacatcccc aagcggggg cagaagaag tccctggta 
      ggggccgcc gagaccgaa tgcgagcta acaccagac 
      agcttgcgc gactcgggt ataagcatg tcggagaac 
      tcaaatttg aataatttg aacaacact tgttatgaa 
      gatttcgat ttgaagggg caccagcag gagatgaac 
      atcagcagc agtccgttc gagggcagc cccaagctg 
      gagaacccg ccgccgatc ccgcccaag gccagcctg 
      agctcgagc ctggaggcg gcgcgggag gcggaggcc 
      gccaccccc gactccgat aaggagaaa agcccctcg 
      taa
    mRNA sequence
    • >TC034453 RA
      gcgttttta attgcacat tttcgggtc taattgtag 
      ctgtaggtt gtgggattt gtgaatggg attgtcaat 
      gttggcctt gaggggccg atgaccctg atggtacca 
      tcggtacca tgactcatg gttcgcttg gttacagga 
      aattgggac tggatctgg tgcccagac aaggggcgc 
      aaatgatcg atccggact cgatgtctg tggtcgaat 
      tgtaccatt ttcatgtct cgagtgccg agaactcgc 
      aagggctgt cggcccgcg gcaccatca agaaaaaag 
      aaccgaaaa aaacgctaa cccaccacc tctacttct 
      gcatgcgcg acttcggcc accacatcg gcgaagaca 
      ccgaaatct acttctctc tgtacgacg cctccaagc 
      agaaataca tcagcgaac gtttcctag tcaaaatat 
      ccaaagagg gtttctcga actacgtgg agaagttgc 
      actccaact gcaccgtct tcacggact tgggcaact 
      ccgacctga gccgggaca tctacctgg tggcccacg 
      tgatgcgaa tcggcaaga tgatctatt ccgacaaca 
      agaagactg aaaagaacg ccgtcctgc tgcagcagg 
      tgttcaagc ggccgcacg gggtcgccg tgcacaacc 
      tgggggagt acctcatgc ccaaggagg gcgactccg 
      aggagaagg agctcaacc tgaaggtgt accaaggcg 
      aggagaagg acttccacc agctgcacg agtttataa 
      taaggcagt cggggaagt acaacgcct tggcttgcg 
      cccccaact atgggatcc tcacctcgg tgaagatgc 
      tccacgggg ggctgaacc acatcaagg aggaaaacg 
      cgatgctgt tcaaaaacg tgagcttga cgagcaagt 
      tggggtttc cggacgtga ttatgccag gggatgtca 
      ggaacgact tgtatctca ttctggaga agggggagt 
      ttgaacgcg ggggtaaat ccacgggga aaaatatcg 
      aagtgagca ttgtggtcc tggacagtg agaaaaatg 
      tcatcagga attgtctgt ggggggctt cggggcaag 
      atggggctt cggaataca attccatga ttatttatc 
      atcacaatt cgccagctt gggccgaaa atgtgaggc 
      tgactctgc cgattgata agttcgcgg gggcgcatg 
      tgaggctgg agtacaggc attgctcaa cacgcgaaa 
      aaaacgaca agaaactct tcggcttct ccttcatcc 
      gtctgatgg ataaagacg gagccgccg tccaagacg 
      gccaacacg aactctaca tttacaaat gtgaagacc 
      cccaaaaac tcgaaaact gcggctatt tgtctctcc 
      cagccttca gcaaagacc tcgaaggca accacgaca 
      gtgtcggcc aattttctc gaagccaca aagaggtgg 
      ttttcgtta gaaccctcc tatgttcca ccaaactga 
      cccaaaacg tcgatttgc tagcactgt tacggtgga 
      aatcccacc cggaaaaaa tccaggact ccttgcagc 
      gcgttttgc gactcggag acgaggaac ttatcaagt 
      tcctccagg atgttctgg atgctctgt ttgcccttt 
      tctcgaccg aagatggca acagtaccg cctacagcg 
      gcctggtgt tccacgttt tggtctcaa tttttaacc 
      tccttgatg gttcaaagt accaacact ttaagccag 
      tcctggatg cgtacatta aggaccatt tcgccgctg 
      ctttggtct acaaaggcc tgctaacaa gtgtgcagc 
      actgtgccg attgggtct tatctttcg acaaacaag 
      aaccgatcc aaaagtgtt acaaaagtc ttgagtaca 
      ttttcaaac tcataatcc agagccggc tgttattct 
      cacgcgcca cgtgtggcc aatacgagg acagcttcc 
      gacgggact tatacagcg ttttttctt cactcaaca 
      aaatgttga cagtgaatg atccccaca ttatcaaca 
      cacaggtgg cacttttac tctcaatta gcgcagtct 
      acgagcaac tgatcgaag ttttgccca cgattgaag 
      taacaaagt tggccgcga ccctcctgg acgccatcc 
      cgaaggact taccggtgc tcctagtcc aggccaaac 
      tatcctgca tcaaaaacc tggtcacaa gcaaattgt 
      ttcaagacg acgagtcca gaaacattc tcctggcaa 
      atgcttgca agcacttgc gtgcgcaac tccaccgtc 
      gggaagaac tcaaacttt gcaccgata ttttgggcg 
      aaatcctgg gatttttgt tcaaacaaa ggaaacagt 
      ttgaaggac aggggaaaa tcaacaatt gcatccacc 
      acgatgttg agacgcttt gtatcaaca ttttggaca 
      ttttggtcc aggctgtgc taacaacca ttgatttta 
      aggacgtca aggacggga aaattctgg gctgtttgg 
      tggcgtgtt tgattggga ttttgcaac ttttggacg 
      agtttcact acaaacggc tgtgggaag tcctaatgg 
      ggccgggcc tggaccgga agcccctca aagacttcc 
      tcttgcgtg ttttcctag ttttccgaa atttggtgc 
      gtttggagg ttttccccc ctgattggt tggtcatga 
      aaatggtgg ttaacaacg ttatgctaa aatcgctac 
      aagagttag cacaacctt tagccttca agtttttgg 
      actcgcgga gcggttact tcgacaaag agctttgga 
      ccgactatt tcaacctcg ctgtggact acttgaccc 
      agaagccgc tccaactgg agcacttct ccgaagtga 
      agcgcgaga aaattattg agaagtacg gcgatatga 
      gggttttga tgggcttcc agatcttgt caatgtggt 
      cgcagttgg gcgaatgca aactacatt ttattccgt 
      cgatggttg gaccgttcc ttaaagtaa cactagttc 
      ctgagattg aattacgaa aggccacac tgcacattt 
      ttttcgaca tgatggagt gcgagcaaa gggcgcgcg 
      ggaatttca gacaggtag agagtgagc tgatcgaca 
      agctggata ttctcatct cggaaaata agggcgatg 
      atgagtacc gcaggcttt tcaatacta tcctgctag 
      accgcgtcc aatcggagg atttggcgt ggaaggaga 
      gcggtgcag ctttcataa gctccatca cacgtctcc 
      ttgaacgcc ttctcgact accgcagcg tcatagaag 
      gcgacgaaa accgcgaca agcgaatgt cttgcactt 
      tcaatcttc tgaatttct acaaaaacg agttcaatc 
      gtaaagaaa tgtatctcc gctacattt ataagctcc 
      acgatcttc atttatcgg cggagaact acacagagg 
      cggcgttta cgatgaaac tgtacgctg atcagctga 
      cttggagta gtaccacac tggtggccg ataatcatt 
      ttccgaatt ttaccgaat gccaggtta aggagaagt 
      tgtacagac agattatta attatttcg ataagggga 
      agtgttggg agaagggga ttcccctgt tgaaggagc 
      tggcaacac tgtacgaag tccagtttt tcgactaca 
      aagctctga gtgatattc ttaaatacc aagcgaaat 
      tttacgata atattttga cgcagctcc gtccagaac 
      cagaatatt ttagggtgg gcttctacg ggcttagct 
      ttccgttgt ttgtgagga ataaacaat ttgtatata 
      ggggattag aatacgaac gcatcggtg cctttactc 
      aaagacttc aaacggagt ttccctcag ctcagatac 
      ttatgaaga acacgccac ccgacgact cgatcgtca 
      actcagagg ggcaattta tacaaattt gcaacgtga 
      aaccaatcc ctgaaccga acccgatta ctctagcga 
      ccgacattc ccgagaaaa tctcccggt tttaccact 
      acaacgacg tgaaaaggt tccagtgcg accgtcctg 
      tacataaag gaattatag ataaggaga atgagatta 
      agactttgt ggatcgaac gcatgactc tggagatcg 
      agaaccccc ttccgggga ttttgcgct ggtttgagg 
      tcatcaatc gccacactg acgaaatac cgcctgtta 
      agtacgcct gcgagacga tgcagtcgg tcgagctcg 
      aattgaggc agttaataa cgacttata cgatcgaac 
      cgaagcgaa atttgaacc cgctgacca tgaggctcc 
      aagggatta ttgatgcga acgtccagg gcgggatta 
      gcaaatacc agcaggcgt ttttcacgc aagaattcg 
      caaaacttt gccctgaac acatgacct atgtttaca 
      ccctcaaaa acctgattc tggacgcta tgcaagtga 
      ttgaggaag ggttagagc tacacggga ggttagcgc 
      cggccggtg tagtccccc tccaccacc ggctcctgg 
      agcgttttg cccaactga aggaaagtc ttggtgctt 
      tgagcaaaa tcaaacgac aaatccccg acagtattg 
      taaacaccc ccctccccc cgttgccga tcgacaaaa 
      gactgatgg tggtggaaa acggccacc accgacccg 
      gaaactacg actacttat ccgaacacc gaacgctgg 
      aaaccgacg acatctaca ccaaaccca tggaaaacg 
      aacggccgc tccgcgaaa acctaacac tgccgatgg 
      ccacgacga cagcgccac ctatacctc aacgcgaac 
      tcagaccca gatctcagg gattcacca ataacttca 
      gcgaggcgc agacgccga ccagactgg actacaaca 
      cgtccagct tgcccttga tgaagacgc gcaactcgg 
      actcgtacg acattccac tgcctccga agccgacgc 
      attcgaggg agcggtcgc tcacgaagc cgccgtcgc 
      cgcggctgc tgaggcacg cctccatga cgccgacgg 
      acgggacgt cgcctttga ggaactcct ggtccgact 
      cgggggtgg aggaggcgc ccccgctgc cgccgaggt 
      cgcacaccc ccgacaagc gtctggggt tcagcgagc 
      cgccgccca acatcccca agcgggggc agaagaagt 
      ccctggtag gggccgccg agaccgaat gcgagctaa 
      caccagaca gcttgcgcg actcgggta taagcatgt 
      cggagaact caaatttga ataatttga acaacactt 
      gttatgaag atttcgatt tgaaggggc accagcagg 
      agatgaaca tcagcagca gtccgttcg agggcagcc 
      ccaagctgg agaacccgc cgccgatcc cgcccaagg 
      ccagcctga gctcgagcc tggaggcgg cgcgggagg 
      cggaggccg ccacccccg actccgata aggagaaaa 
      gcccctcgt aataattat ttcatgttg tgatcgtaa 
      acgtagtca gggcagttg tgagagttg cagtatttt 
      atttattca ttccaggag atgaaatgt ttcgagtat 
      tttaatcga taggtgtta ggtttagtg tctcattgt 
      gtcatttcc ttgtgccaa agcaggccg ttatatcaa 
      agcaggttc atggaccaa tggaggaac taatggacc 
      gatccactc actcattct acatcgaag ttttgtata 
      taatggtgc taatttttt tatctattc tacgtatca 
      tggtatatc cgttcaatt ggttgtatt cgtcatttt 
      aaccatcgg actagtcat aaaaatgat atttataat 
      tatatttta tatctgcat aaagtattg tattttccc 
      attcatgac tatcatcta gttagagat ttactgtag 
      ctgaaaaaa taaagcgga aaatagcac
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