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Gene information
General information
TC011732 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14259379..14279035
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011732
    iBeetle screen iB_06522

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011732 RA
      MFLLAAVSL LLSAGLAGS MPGGPGVPE NITVTFLNP 
      TSVKVSWST SVENVDKYD VTYKPTDAS YRVVAVVAG 
      NSDAVTLSG LKADTQYQL TVTAVWARK RYRSRPIVF 
      RTLEPPRSS PQQDPMATL PTDAPPTRD DAEPVDLPE 
      YTYPQTSST VRGVEIGIV LLVLLVWVA AIALFFNRW 
      GKIRMLLPY QPDYKQEQL KVPGTAACA AAVASGNTC 
      THHSGDHLC QQCEETEEA TIMASVPIK DIADNPCPP 
      APRKHRPRI NSAIFVSNS GPGLDTREF FRRHGSVSR 
      LCRKARSVD NISLEENHF GLPTLSVSG PSPPEDDDR 
      DDVQETQRF LE
    • >TC011732 RB
      MPGGPGVPE NITVTFLNP TSVKVSWST SVENVDKYD 
      VTYKPTDAR VVAVVAGNS DAVTLSGLK ADTQYQLTV 
      TAVWARKRY RSRPIVFRT LEPPRSSPQ QDPMATLPT 
      DAPPTRDDA EPVDLPEYT YPQTSSTVR GVEIGIVLL 
      VLLVWVAAI ALFFNRWGK IRMLLPYQP DYKQEQLKV 
      PGTAACAAA VASGNTCTH HSGDHLCQQ CEETEEATI 
      MASVPIKDI ADNPCPPAP RKHRPRINS AIFVSNSGP 
      GLDTREFFR RHGSVSRLC RKARSVDNI SLEENHFGL 
      PTLSVSGPS PPEDDDRDD VQETQRFLE
    • >TC011732 RC
      MPGGPGVPE NITVTFLNP TSVKVSWST SVENVDKYD 
      VTYKPTDAS YRVVAVVAG NSDAVTLSG LKADTQYQL 
      TVTAVWARK RYRSRPIVF RTLEPPRSS PQQDPMATL 
      PTDAPPTRD DAEPVDLPE YTYPQTSST VRGVEIGIV 
      LLVLLVWVA AIALFFNRW GKIRMLLPY QPDYKQEQL 
      KVPGTAACA AAVASGNTC THHSGDHLC QQCEETEEA 
      TIMASVPIK DIADNPCPP APRKHRPRI NSAIFVSNS 
      GPGLDTREF FRRHGSVSR LCRKARSVD NISLEENHF 
      GLPTLSVSG PSPPEDDDR DDVQETQRF LE
    CDS sequence
    • >TC011732 RA
      atgttctta ttggctgcc gtctcattg ctactaagt 
      gccggatta gcaggttcg atgcccggt ggtcccggg 
      gtccccgag aacatcacc gtaactttc ctcaatccg 
      acttccgtg aaggtgtct tggtcgaca tccgtcgaa 
      aatgtggac aagtacgac gtcacttac aaaccgact 
      gatgccagt tacagagtg gtggcggtg gttgcggga 
      aattcggac gctgtgact ttaagtgga ttaaaagcg 
      gacactcag tatcaattg actgtgacc gccgtttgg 
      gcccgcaag agatacaga agtaggcca atcgtcttc 
      cgcacattg gagccgccg cgctcctcc ccgcagcag 
      gaccccatg gcgacgctt ccgacggac gccccgccg 
      acgagggac gacgccgag cccgtcgac ttgcccgag 
      tacacgtac ccgcaaacg agctcaacg gtgagaggc 
      gtcgagatc ggaatcgtc ctcctggtg ctgctcgtc 
      tgggtggcg gcgatagct ttgttcttc aatcgatgg 
      ggcaaaatc cggatgctg cttccctat cagccggat 
      tacaaacag gagcagctc aaggtgccg gggacggcg 
      gcctgtgcg gcggcggtc gcatcgggc aacacctgc 
      acacatcat tccggcgac catctctgc cagcagtgc 
      gaagagacg gaagaagcc acaattatg gcttcggtt 
      ccaattaaa gacatcgct gataatccg tgtcctcct 
      gcgcctcgt aaacaccgt ccccgcata aactcggcc 
      attttcgta tcgaacagc ggtccaggt ctggatacg 
      agggagttt ttcaggcgc cacggctcc gtctcccgt 
      ctgtgtagg aaggcgcgc agcgtggac aacatctcc 
      ttggaggag aaccacttc gggctgccg acgctgtcg 
      gtgtcgggg ccgtcgccg cccgaagac gacgacagg 
      gacgatgtc caagagacc cagcgcttt ctggagtaa
    • >TC011732 RB
      atgcccggt ggtcccggg gtccccgag aacatcacc 
      gtaactttc ctcaatccg acttccgtg aaggtgtct 
      tggtcgaca tccgtcgaa aatgtggac aagtacgac 
      gtcacttac aaaccgact gatgccaga gtggtggcg 
      gtggttgcg ggaaattcg gacgctgtg actttaagt 
      ggattaaaa gcggacact cagtatcaa ttgactgtg 
      accgccgtt tgggcccgc aagagatac agaagtagg 
      ccaatcgtc ttccgcaca ttggagccg ccgcgctcc 
      tccccgcag caggacccc atggcgacg cttccgacg 
      gacgccccg ccgacgagg gacgacgcc gagcccgtc 
      gacttgccc gagtacacg tacccgcaa acgagctca 
      acggtgaga ggcgtcgag atcggaatc gtcctcctg 
      gtgctgctc gtctgggtg gcggcgata gctttgttc 
      ttcaatcga tggggcaaa atccggatg ctgcttccc 
      tatcagccg gattacaaa caggagcag ctcaaggtg 
      ccggggacg gcggcctgt gcggcggcg gtcgcatcg 
      ggcaacacc tgcacacat cattccggc gaccatctc 
      tgccagcag tgcgaagag acggaagaa gccacaatt 
      atggcttcg gttccaatt aaagacatc gctgataat 
      ccgtgtcct cctgcgcct cgtaaacac cgtccccgc 
      ataaactcg gccattttc gtatcgaac agcggtcca 
      ggtctggat acgagggag tttttcagg cgccacggc 
      tccgtctcc cgtctgtgt aggaaggcg cgcagcgtg 
      gacaacatc tccttggag gagaaccac ttcgggctg 
      ccgacgctg tcggtgtcg gggccgtcg ccgcccgaa 
      gacgacgac agggacgat gtccaagag acccagcgc 
      tttctggag taa
    • >TC011732 RC
      atgcccggt ggtcccggg gtccccgag aacatcacc 
      gtaactttc ctcaatccg acttccgtg aaggtgtct 
      tggtcgaca tccgtcgaa aatgtggac aagtacgac 
      gtcacttac aaaccgact gatgccagt tacagagtg 
      gtggcggtg gttgcggga aattcggac gctgtgact 
      ttaagtgga ttaaaagcg gacactcag tatcaattg 
      actgtgacc gccgtttgg gcccgcaag agatacaga 
      agtaggcca atcgtcttc cgcacattg gagccgccg 
      cgctcctcc ccgcagcag gaccccatg gcgacgctt 
      ccgacggac gccccgccg acgagggac gacgccgag 
      cccgtcgac ttgcccgag tacacgtac ccgcaaacg 
      agctcaacg gtgagaggc gtcgagatc ggaatcgtc 
      ctcctggtg ctgctcgtc tgggtggcg gcgatagct 
      ttgttcttc aatcgatgg ggcaaaatc cggatgctg 
      cttccctat cagccggat tacaaacag gagcagctc 
      aaggtgccg gggacggcg gcctgtgcg gcggcggtc 
      gcatcgggc aacacctgc acacatcat tccggcgac 
      catctctgc cagcagtgc gaagagacg gaagaagcc 
      acaattatg gcttcggtt ccaattaaa gacatcgct 
      gataatccg tgtcctcct gcgcctcgt aaacaccgt 
      ccccgcata aactcggcc attttcgta tcgaacagc 
      ggtccaggt ctggatacg agggagttt ttcaggcgc 
      cacggctcc gtctcccgt ctgtgtagg aaggcgcgc 
      agcgtggac aacatctcc ttggaggag aaccacttc 
      gggctgccg acgctgtcg gtgtcgggg ccgtcgccg 
      cccgaagac gacgacagg gacgatgtc caagagacc 
      cagcgcttt ctggagtaa
    mRNA sequence
    • >TC011732 RA
      cctgaaaaa gccctcagt tacgtcgcg acgctcgtc 
      atgaggagg tcacatgac tcggccaca cgtcgatat 
      aaatcaaat aatgatgga tgtgttttt gtgagcggc 
      ggctgaaaa attttcaag cgtttcacg tccggaaaa 
      agacggttt tgtggtggg tttggtgac agtgggggg 
      agatcatgc gaaggcgga agcattccg gattcttcc 
      agtgaccga atccgggaa agcgtcgcc gccatgtta 
      ggggcaaca ccggatttc acgtgcagc taatatgtt 
      cttattggc tgccgtctc attgctact aagtgccgg 
      attagcagg ttcgatgcc cggtggtcc cggggtccc 
      cgagaacat caccgtaac tttcctcaa tccgacttc 
      cgtgaaggt gtcttggtc gacatccgt cgaaaatgt 
      ggacaagta cgacgtcac ttacaaacc gactgatgc 
      cagttacag agtggtggc ggtggttgc gggaaattc 
      ggacgctgt gactttaag tggattaaa agcggacac 
      tcagtatca attgactgt gaccgccgt ttgggcccg 
      caagagata cagaagtag gccaatcgt cttccgcac 
      attggagcc gccgcgctc ctccccgca gcaggaccc 
      catggcgac gcttccgac ggacgcccc gccgacgag 
      ggacgacgc cgagcccgt cgacttgcc cgagtacac 
      gtacccgca aacgagctc aacggtgag aggcgtcga 
      gatcggaat cgtcctcct ggtgctgct cgtctgggt 
      ggcggcgat agctttgtt cttcaatcg atggggcaa 
      aatccggat gctgcttcc ctatcagcc ggattacaa 
      acaggagca gctcaaggt gccggggac ggcggcctg 
      tgcggcggc ggtcgcatc gggcaacac ctgcacaca 
      tcattccgg cgaccatct ctgccagca gtgcgaaga 
      gacggaaga agccacaat tatggcttc ggttccaat 
      taaagacat cgctgataa tccgtgtcc tcctgcgcc 
      tcgtaaaca ccgtccccg cataaactc ggccatttt 
      cgtatcgaa cagcggtcc aggtctgga tacgaggga 
      gtttttcag gcgccacgg ctccgtctc ccgtctgtg 
      taggaaggc gcgcagcgt ggacaacat ctccttgga 
      ggagaacca cttcgggct gccgacgct gtcggtgtc 
      ggggccgtc gccgcccga agacgacga cagggacga 
      tgtccaaga gacccagcg ctttctgga gtaaatagg 
      ggtgcgagt taggtgcca atgcagtga cgctccttg 
      gatcggtgg tagtctaat tatacttag aactttgcc 
      attaggttt agtgtcgat actgtactg acaactacg 
      agctttgta cgaacattg accttcgga cacacatat 
      cgggtttga tttcaggaa ttttttaag aaacgggac 
      caaagtcgg caaccagag ctttaatcg tgggccaca 
      gcgagcggg gggaggtgt tcgtgcaat ttctacaat 
      ttttctata gctttaact actctaggg attagttac 
      gaggtccaa acactgcta ttttcaagc cctgagttg 
      gagcaaatg tgcacggaa gccgaaagt cacgattta 
      actcaaaaa aaatcattt gtgttttag ctttaaatt 
      cttatcgtg tgttattta ggtgacttt cagggcttc 
      cgtaatgtg taattaatt catcgaatt actaggaat 
      aataataat aatggatgg cttttaaca aacagctct 
      cgatcaaat tcaattcta ccacttgcc tgtatcaac 
      gatcacttg ctcaaaact ctgaatgtc aacactgga 
      gaagttatt ttgacgata tatactata cttttatac 
      gtagacgta cttcctaac aatttcatt gtgtttcgg 
      tctatttat gatagttta ttattgttg tttttcatt 
      atcattagt ctatctttt ctacgaatt gtgtcataa 
      taatagaat ataatcgtc gtgtcactt ggaggaaat 
      atcatgtgt atatatcat cgttttgaa tttatcaat 
      tggctttac ataatgggt ccaatactg acttgtaga 
      acccaaatt tgtctacag ttttttttc taatcactg 
      tgattttaa tgccttcat ttactttac aaaaacact 
      gtcgaggta aagggtctt gtaaaatgc tgtatataa 
      atttatcac agatttctt tgtgtaaac gcaataaat 
      taataccta aacg
    • >TC011732 RB
      acaactgtc atccacaga gagggattc gaattgtta 
      gcaacagtt ctaatcaaa taagacaag aattgaagc 
      cggaaaatt tcatttgct cgcaagctg gaatttaat 
      gaacaacta gctattttt gctcagttc ttgatgctt 
      tatgtctgt tttttatcc caaaatccc actataatc 
      actccactt ttttccaca aaattaaac aattcccgg 
      aagccttcc aggtcttgg tcatttgtt tcttttgca 
      gttaaaaaa aatccacga aaaaaatcg ccgtgttat 
      tagtttttg agatttgta gtttttcag ggagatcat 
      gcgaaggcg gaagcattc cggattctt ccagtgacc 
      gaatccggg aaagcgtcg ccgccatgt taggggcaa 
      caccggatt tcacgtgca gctaatatg ttcttattg 
      gctgccgtc tcattgcta ctaagtgcc ggattagca 
      ggttcgatg cccggtggt cccggggtc cccgagaac 
      atcaccgta actttcctc aatccgact tccgtgaag 
      gtgtcttgg tcgacatcc gtcgaaaat gtggacaag 
      tacgacgtc acttacaaa ccgactgat gccagagtg 
      gtggcggtg gttgcggga aattcggac gctgtgact 
      ttaagtgga ttaaaagcg gacactcag tatcaattg 
      actgtgacc gccgtttgg gcccgcaag agatacaga 
      agtaggcca atcgtcttc cgcacattg gagccgccg 
      cgctcctcc ccgcagcag gaccccatg gcgacgctt 
      ccgacggac gccccgccg acgagggac gacgccgag 
      cccgtcgac ttgcccgag tacacgtac ccgcaaacg 
      agctcaacg gtgagaggc gtcgagatc ggaatcgtc 
      ctcctggtg ctgctcgtc tgggtggcg gcgatagct 
      ttgttcttc aatcgatgg ggcaaaatc cggatgctg 
      cttccctat cagccggat tacaaacag gagcagctc 
      aaggtgccg gggacggcg gcctgtgcg gcggcggtc 
      gcatcgggc aacacctgc acacatcat tccggcgac 
      catctctgc cagcagtgc gaagagacg gaagaagcc 
      acaattatg gcttcggtt ccaattaaa gacatcgct 
      gataatccg tgtcctcct gcgcctcgt aaacaccgt 
      ccccgcata aactcggcc attttcgta tcgaacagc 
      ggtccaggt ctggatacg agggagttt ttcaggcgc 
      cacggctcc gtctcccgt ctgtgtagg aaggcgcgc 
      agcgtggac aacatctcc ttggaggag aaccacttc 
      gggctgccg acgctgtcg gtgtcgggg ccgtcgccg 
      cccgaagac gacgacagg gacgatgtc caagagacc 
      cagcgcttt ctggagtaa ataggggtg cgagttagg 
      tgccaatgc agtgacgct ccttggatc ggtggtagt 
      ctaattata cttagaact ttgccatta ggtttagtg 
      tcgatactg tactgacaa ctacgagct ttgtacgaa 
      cattgacct tcggacaca catatcggg tttgatttc 
      aggaatttt ttaagaaac gggaccaaa gtcggcaac 
      cagagcttt aatcgtggg ccacagcga gcgggggga 
      ggtgttcgt gcaatttct acaattttt ctatagctt 
      taactactc tagggatta gttacgagg tccaaacac 
      tgctatttt caagccctg agttggagc aaatgtgca 
      cggaagccg aaagtcacg atttaactc aaaaaaaat 
      catttgtgt tttagcttt aaattctta tcgtgtgtt 
      atttaggtg actttcagg gcttccgta atgtgtaat 
      taattcatc gaattacta ggaataata ataataatg 
      gatggcttt taacaaaca gctctcgat caaattcaa 
      ttctaccac ttgcctgta tcaacgatc acttgctca 
      aaactctga atgtcaaca ctggagaag ttattttga 
      cgatatata ctatacttt tatacgtag acgtacttc 
      ctaacaatt tcattgtgt ttcggtcta tttatgata 
      gtttattat tgttgtttt tcattatca ttagtctat 
      cttttctac gaattgtgt cataataat agaatataa 
      tcgtcgtgt cacttggag gaaatatca tgtgtatat 
      atcatcgtt ttgaattta tcaattggc tttacataa 
      tgggtccaa tactgactt gtagaaccc aaatttgtc 
      tacagtttt ttttctaat cactgtgat tttaatgcc 
      ttcatttac tttacaaaa acactgtcg aggtaaagg 
      gtcttgtaa aatgctgta tataaattt atcacagat 
      ttctttgtg taaacgcaa taaattaat acctaaacg
    • >TC011732 RC
      acaactgtc atccacaga gagggattc gaattgtta 
      gcaacagtt ctaatcaaa taagacaag aattgaagc 
      cggaaaatt tcatttgct cgcaagctg gaatttaat 
      gaacaacta gctattttt gctcagttc ttgatgctt 
      tatgtctgt tttttatcc caaaatccc actataatc 
      actccactt ttttccaca aaattaaac aattcccgg 
      aagccttcc aggtcttgg tcatttgtt tcttttgca 
      gttaaaaaa aatccacga aaaaaatcg ccgtgttat 
      tagtttttg agatttgta gtttttcag ggagatcat 
      gcgaaggcg gaagcattc cggattctt ccagtgacc 
      gaatccggg aaagcgtcg ccgccatgt taggggcaa 
      caccggatt tcacgtgca gctaatatg ttcttattg 
      gctgccgtc tcattgcta ctaagtgcc ggattagca 
      ggttcgatg cccggtggt cccggggtc cccgagaac 
      atcaccgta actttcctc aatccgact tccgtgaag 
      gtgtcttgg tcgacatcc gtcgaaaat gtggacaag 
      tacgacgtc acttacaaa ccgactgat gccagttac 
      agagtggtg gcggtggtt gcgggaaat tcggacgct 
      gtgacttta agtggatta aaagcggac actcagtat 
      caattgact gtgaccgcc gtttgggcc cgcaagaga 
      tacagaagt aggccaatc gtcttccgc acattggag 
      ccgccgcgc tcctccccg cagcaggac cccatggcg 
      acgcttccg acggacgcc ccgccgacg agggacgac 
      gccgagccc gtcgacttg cccgagtac acgtacccg 
      caaacgagc tcaacggtg agaggcgtc gagatcgga 
      atcgtcctc ctggtgctg ctcgtctgg gtggcggcg 
      atagctttg ttcttcaat cgatggggc aaaatccgg 
      atgctgctt ccctatcag ccggattac aaacaggag 
      cagctcaag gtgccgggg acggcggcc tgtgcggcg 
      gcggtcgca tcgggcaac acctgcaca catcattcc 
      ggcgaccat ctctgccag cagtgcgaa gagacggaa 
      gaagccaca attatggct tcggttcca attaaagac 
      atcgctgat aatccgtgt cctcctgcg cctcgtaaa 
      caccgtccc cgcataaac tcggccatt ttcgtatcg 
      aacagcggt ccaggtctg gatacgagg gagtttttc 
      aggcgccac ggctccgtc tcccgtctg tgtaggaag 
      gcgcgcagc gtggacaac atctccttg gaggagaac 
      cacttcggg ctgccgacg ctgtcggtg tcggggccg 
      tcgccgccc gaagacgac gacagggac gatgtccaa 
      gagacccag cgctttctg gagtaaata ggggtgcga 
      gttaggtgc caatgcagt gacgctcct tggatcggt 
      ggtagtcta attatactt agaactttg ccattaggt 
      ttagtgtcg atactgtac tgacaacta cgagctttg 
      tacgaacat tgaccttcg gacacacat atcgggttt 
      gatttcagg aatttttta agaaacggg accaaagtc 
      ggcaaccag agctttaat cgtgggcca cagcgagcg 
      gggggaggt gttcgtgca atttctaca atttttcta 
      tagctttaa ctactctag ggattagtt acgaggtcc 
      aaacactgc tattttcaa gccctgagt tggagcaaa 
      tgtgcacgg aagccgaaa gtcacgatt taactcaaa 
      aaaaatcat ttgtgtttt agctttaaa ttcttatcg 
      tgtgttatt taggtgact ttcagggct tccgtaatg 
      tgtaattaa ttcatcgaa ttactagga ataataata 
      ataatggat ggcttttaa caaacagct ctcgatcaa 
      attcaattc taccacttg cctgtatca acgatcact 
      tgctcaaaa ctctgaatg tcaacactg gagaagtta 
      ttttgacga tatatacta tacttttat acgtagacg 
      tacttccta acaatttca ttgtgtttc ggtctattt 
      atgatagtt tattattgt tgtttttca ttatcatta 
      gtctatctt ttctacgaa ttgtgtcat aataataga 
      atataatcg tcgtgtcac ttggaggaa atatcatgt 
      gtatatatc atcgttttg aatttatca attggcttt 
      acataatgg gtccaatac tgacttgta gaacccaaa 
      tttgtctac agttttttt tctaatcac tgtgatttt 
      aatgccttc atttacttt acaaaaaca ctgtcgagg 
      taaagggtc ttgtaaaat gctgtatat aaatttatc 
      acagatttc tttgtgtaa acgcaataa attaatacc 
      taaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY