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Gene information
General information
TC034891 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14216091..14217438
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034891
    iBeetle screen iB_06520

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034891 RA
      MNNLEIPAD SAPEFTSTS ETSLLVVIV DSNPSQKML 
      RDNPHVLTH CIDSVIAFA NSHLMQKAQ NKLAVMACH 
      SKTSEFIYP GPNKPLDVR QIDGQYEVF TAVEKTIKQ 
      NLAKLLATE KSTLVTESL LAGAIAMAL CYIARIQRT 
      KPPGCVLNS RILVVTGSG DSASQYMNY MNVFFTAQK 
      QGIVIDVCA LDQHLSLLQ QGCDITGGL YLKVPQLQG 
      LLQYLLWVF LPEPPIRQK LVLPPPVKV DYRAACFCH 
      RELIDIGYV CSVCLSIFC KFSPICTTC HTVFKIPGP 
      LAVKPKKRK IKL
    CDS sequence
    • >TC034891 RA
      atgaataat ttggagatc ccggccgat tcggccccc 
      gaattcacc tcaaccagc gagactagt ctcctggtg 
      gtaatcgtc gacagtaac cccagccag aagatgctc 
      cgagacaac ccccacgtc ctcacccac tgcatagac 
      tcagtcata gcgttcgcc aactctcat ttaatgcaa 
      aaggcgcaa aacaagctc gccgtgatg gcttgccac 
      tccaagacc agcgagttc atctacccg ggccccaac 
      aagcccctc gacgtgcgc caaatcgac ggccagtac 
      gaggttttc acggcagtg gagaaaaca atcaagcag 
      aacttggcc aaattgctg gccaccgaa aaatcaact 
      cttgtgacg gagtcactg ctcgcaggg gcgatcgct 
      atggccctg tgttacatt gcgcggatc cagcggacg 
      aagcccccc gggtgtgtc ctaaacagc cgcattttg 
      gtggtgacg gggagcgga gactccgcc tcgcagtat 
      atgaattat atgaacgtt tttttcacg gcgcaaaaa 
      cagggaatt gtgattgat gtgtgcgcc cttgaccaa 
      catttgagt ctgctgcag caaggctgc gatatcaca 
      ggggggctg tacttgaaa gtcccgcaa ctgcaaggc 
      ctgttacaa tatcttctc tgggtgttt ttgcctgag 
      ccgcctatt cggcaaaaa ttggtgctt ccgccccct 
      gtcaaggtt gactacagg gcggcttgt ttctgccac 
      cgggagtta attgatata ggttacgtc tgttcggtg 
      tgtttgtca attttctgc aagtttagc ccgatttgt 
      acgacttgt catactgtc ttcaaaatc ccaggaccg 
      ttagctgtg aagccgaaa aagcggaaa atcaagttg 
      taa
    mRNA sequence
    • >TC034891 RA
      actcaattc ccatataac cccactttt aaaccgcac 
      ataaacaat gaataattt ggagatccc ggccgattc 
      ggcccccga attcacctc aaccagcga gactagtct 
      cctggtggt aatcgtcga cagtaaccc cagccagaa 
      gatgctccg agacaaccc ccacgtcct cacccactg 
      catagactc agtcatagc gttcgccaa ctctcattt 
      aatgcaaaa ggcgcaaaa caagctcgc cgtgatggc 
      ttgccactc caagaccag cgagttcat ctacccggg 
      ccccaacaa gcccctcga cgtgcgcca aatcgacgg 
      ccagtacga ggttttcac ggcagtgga gaaaacaat 
      caagcagaa cttggccaa attgctggc caccgaaaa 
      atcaactct tgtgacgga gtcactgct cgcaggggc 
      gatcgctat ggccctgtg ttacattgc gcggatcca 
      gcggacgaa gccccccgg gtgtgtcct aaacagccg 
      cattttggt ggtgacggg gagcggaga ctccgcctc 
      gcagtatat gaattatat gaacgtttt tttcacggc 
      gcaaaaaca gggaattgt gattgatgt gtgcgccct 
      tgaccaaca tttgagtct gctgcagca aggctgcga 
      tatcacagg ggggctgta cttgaaagt cccgcaact 
      gcaaggcct gttacaata tcttctctg ggtgttttt 
      gcctgagcc gcctattcg gcaaaaatt ggtgcttcc 
      gccccctgt caaggttga ctacagggc ggcttgttt 
      ctgccaccg ggagttaat tgatatagg ttacgtctg 
      ttcggtgtg tttgtcaat tttctgcaa gtttagccc 
      gatttgtac gacttgtca tactgtctt caaaatccc 
      aggaccgtt agctgtgaa gccgaaaaa gcggaaaat 
      caagttgta ataaaaatt ttttttgtt gaatttgtt 
      ttattatta cccttgata atgataaat gcgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY