Status
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Gene information
General information
TC034446
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011742,TC011741
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14203030..14210100
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034446
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06519

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages random shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    Pupal morphology
    female genital lobes shortened - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034446 RA
      MDAWGTLDI TSAITRDDL NSSFRRSKK ITIEKLPDK 
      VLLHIFSYL SHKEICRMA KVCKKWRMI AYDTRLWKN 
      VSLRPEISG LHVGSLESL LALISIRFG PSLRYIELP 
      IELITHTVL HELANKCPN LTHMLLDFS TAMQLHDFS 
      ELQAFPTKL RYLCICLSE VIFMEGFMR KIYNFINGL 
      EILHLIGTY EKVEEEEEE IYEVINVHK LKSATPNLR 
      VINLYGINF VDDSHIDAF SSNCIQLEC LAVNYCAKV 
      TGSTLKTLF QRSRRLKCL LMNGTNLQS DYLMQVEWE 
      KCSLQELDI TATDLSTEA LQDMLTRLP GLRFLSAGQ 
      INGFNDSVL KAWMEAGNP RNLVALDLD SSDNLSEDA 
      IYKFLSKYG PQLWGLSLS GMTHITDSL WQTVLPILT 
      NAKILIMGT QERLGVNIL VDQLMDSIA RNCINVERL 
      ELRWDPDNL RFSDKSQKA IDTLRVKCL KLKCLSLSD 
      GRYYEIVKA NFERADRMT VVRTLTNCR VSNYYLLSN 
      YKDLVFN
    CDS sequence
    • >TC034446 RA
      atggacgcc tggggcacc ctcgacatc acctccgcc 
      atcacgcgg gacgacctc aacagctcg ttccgacgc 
      tcaaaaaaa attaccatc gagaagctc cccgacaaa 
      gtcctgctc cacattttc agctacttg agccacaag 
      gagatctgc cgcatggcc aaagtgtgc aaaaagtgg 
      cgaatgatc gcctacgac acgcgtctc tggaaaaac 
      gtgtcactg agaccagaa atctcgggc ctccatgtc 
      ggctctctg gagtcgctc ttagccttg ataagtatc 
      cgcttcggc ccttctttg cgctacatc gagctgcca 
      atcgagctc ataacgcac acggttttg cacgagctg 
      gccaacaag tgccccaat ttgactcac atgctgttg 
      gacttctcc acagcgatg caacttcac gatttcagc 
      gagttgcaa gcgtttccg accaagttg aggtatctg 
      tgcatttgt ctgagtgag gtcatcttc atggagggc 
      ttcatgcgg aagatttac aattttatt aatgggctt 
      gagattctt cacctgatc gggacctat gagaaggtg 
      gaggaggag gaagaggag atttatgaa gttatcaat 
      gtgcataag ctcaagtcg gcgactccc aatttgaga 
      gttattaac ttgtatggg attaatttt gtggatgat 
      agtcatatt gatgctttc agctcaaat tgtattcag 
      cttgagtgc ctggctgtg aactactgt gccaaagtg 
      accggttcc accctcaaa accctgttc caaaggagt 
      cgtcgcctc aaatgcctc ctaatgaac ggcacaaac 
      ttgcaaagc gattatttg atgcaagtc gagtgggag 
      aaatgctcg cttcaagaa ctcgacatc accgccaca 
      gatctatca actgaggcc ctccaggac atgctgact 
      cgcctccca ggcctcaga ttcctgagc gcgggccaa 
      atcaacggt ttcaacgac tcggtccta aaagcctgg 
      atggaggcc ggaaacccg cgaaatttg gtcgctttg 
      gacctcgac agctcggat aatttgagc gaggacgcg 
      atctataag ttcctgtcc aagtatggg ccccagtta 
      tggggcctg agcctgtct ggaatgacc cacataacc 
      gactcttta tggcagacg gttttgccc attttgacc 
      aacgcaaag attttgatc atggggacg caggagcgg 
      ctcggtgtc aatatcctt gtggatcag ttgatggac 
      agtatcgcc cggaattgc atcaatgtg gagcggttg 
      gagttgagg tgggatccc gataatttg aggttttcg 
      gataagagt cagaaggcg attgatacg ttgagggtc 
      aagtgtttg aagttgaag tgtcttagt ttgagtgat 
      gggcggtat tacgagatt gttaaagcc aattttgag 
      cgagctgat cggatgact gtggtcagg actttgacg 
      aattgtcgg gtctcgaac tattatctc ctctcaaat 
      tacaaggac ttggttttc aactaa
    mRNA sequence
    • >TC034446 RA
      gccggtata ccaaatttt aattaaaaa aattaatca 
      acctaaatg aatgcaaac tttggcata aaattatta 
      taaaccaat tcaaataaa cattttatt gcttccggt 
      ggtttgaat ggcctgggc cccgcaaac ctccaaaaa 
      gtccctcct caagtcaca ataaactaa aaataggct 
      tacagccta gaaaaacgt taatcaaat taatttcaa 
      aacagactt agtgtttgg caagtgaaa gtcaagtgt 
      ggtgtgctt tgtctcgtt gatttattt ttcgaattt 
      ttgtctgag tgttaaatt gagagtttg gacaattac 
      ataaaagtt aagtactca ttaaagcga aaatagcat 
      ttgaagact ggatgagtc tttgtcccg attgattcc 
      tccaaaaat actcgccgc ccaattagt ctcgcgccg 
      ttaattaag ccggtttaa gcccttgcc aattatcct 
      agtgcacgt tggacatcg gcagagatg gacgcctgg 
      ggcaccctc gacatcacc tccgccatc acgcgggac 
      gacctcaac agctcgttc cgacgctca aaaaaaatt 
      accatcgag aagctcccc gacaaagtc ctgctccac 
      attttcagc tacttgagc cacaaggag atctgccgc 
      atggccaaa gtgtgcaaa aagtggcga atgatcgcc 
      tacgacacg cgtctctgg aaaaacgtg tcactgaga 
      ccagaaatc tcgggcctc catgtcggc tctctggag 
      tcgctctta gccttgata agtatccgc ttcggccct 
      tctttgcgc tacatcgag ctgccaatc gagctcata 
      acgcacacg gttttgcac gagctggcc aacaagtgc 
      cccaatttg actcacatg ctgttggac ttctccaca 
      gcgatgcaa cttcacgat ttcagcgag ttgcaagcg 
      tttccgacc aagttgagg tatctgtgc atttgtctg 
      agtgaggtc atcttcatg gagggcttc atgcggaag 
      atttacaat tttattaat gggcttgag attcttcac 
      ctgatcggg acctatgag aaggtggag gaggaggaa 
      gaggagatt tatgaagtt atcaatgtg cataagctc 
      aagtcggcg actcccaat ttgagagtt attaacttg 
      tatgggatt aattttgtg gatgatagt catattgat 
      gctttcagc tcaaattgt attcagctt gagtgcctg 
      gctgtgaac tactgtgcc aaagtgacc ggttccacc 
      ctcaaaacc ctgttccaa aggagtcgt cgcctcaaa 
      tgcctccta atgaacggc acaaacttg caaagcgat 
      tatttgatg caagtcgag tgggagaaa tgctcgctt 
      caagaactc gacatcacc gccacagat ctatcaact 
      gaggccctc caggacatg ctgactcgc ctcccaggc 
      ctcagattc ctgagcgcg ggccaaatc aacggtttc 
      aacgactcg gtcctaaaa gcctggatg gaggccgga 
      aacccgcga aatttggtc gctttggac ctcgacagc 
      tcggataat ttgagcgag gacgcgatc tataagttc 
      ctgtccaag tatgggccc cagttatgg ggcctgagc 
      ctgtctgga atgacccac ataaccgac tctttatgg 
      cagacggtt ttgcccatt ttgaccaac gcaaagatt 
      ttgatcatg gggacgcag gagcggctc ggtgtcaat 
      atccttgtg gatcagttg atggacagt atcgcccgg 
      aattgcatc aatgtggag cggttggag ttgaggtgg 
      gatcccgat aatttgagg ttttcggat aagagtcag 
      aaggcgatt gatacgttg agggtcaag tgtttgaag 
      ttgaagtgt cttagtttg agtgatggg cggtattac 
      gagattgtt aaagccaat tttgagcga gctgatcgg 
      atgactgtg gtcaggact ttgacgaat tgtcgggtc 
      tcgaactat tatctcctc tcaaattac aaggacttg 
      gttttcaac taagaataa ggcgtgtgt ttataattt 
      tgtaaataa ctgtaatta aacaaggaa agttgga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY