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Gene information
General information
TC011760 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13866722..13875520
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011760
      iBeetle screen iB_06516

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011760 RA
        MIAAAGLLL LVPLAGCAH IASLNIEQH QGNYSQVMP 
        FLFNQTTEH VLVFPTSDS IYPYRVKAW SSGAKLASP 
        VLVVVRQER EVISWQVPF VVDTTMKEG VVHFHNTSR 
        TLCHNDMPR IAKAKATSR ILPIQLSQN FIIALSTSS 
        LVNVDISVM VEEERDFYL QEGRPYEVS VSPSESKYY 
        YYKFHDKKN TSAMIEINS DDDVCLTVS IQDSFCPVL 
        DLDKDITYE GKYQTINRK GGMTIRQRE FPDGFFLVF 
        VAKADNYQC SQKHSVLLV EHRKQHLIL ANRTSTITF 
        TINKGINGK EYEIASLAT LGALLSFCI VSTIMIFAF 
        TRWGTISKF RPSGDELDA DWKEPPEPP ITRELKHEL 
        LSRQALTVN LLARAPEKD KRRSYNYLW HILSIAIFY 
        SIPVVQLVI TYQRVVNRT GDQDMCYYN FLCANPAFG 
        LSDFNHIFS NVGYIIVGI LFLGVVLHR QTKIPNSST 
        GIPVHYGVY YAMGIALII EGILSACYH ICPSQSNYQ 
        FDTSFMYVM AVLCMIKLY QNRHPDVNA TAYATFTVL 
        GMAIFLAMI GILNGSLTV WIVFVVIYS LLCAYISFK 
        IYFISFVFD GFKQLKQSL KSSNKVEAI APIRKSRFA 
        LLVIANIIN YAMLITGLC LYNTGVTDF GTFLLGLLM 
        GNSVLYAVF YTGMKLVNG ERICFEAII YGLLAIAAW 
        ATATVYFLD NATLWTVTP AESRQWNQE CIVMSFYDK 
        HDVWHLLSA PALYLTFMF LLSLDDDLV DTKREEITV 
        F
      CDS sequence
      • >TC011760 RA
        atgatcgcc gccgccggc ctgctcctg ctggtcccc 
        ctcgcgggc tgcgcccac atcgcctcc ctcaacatc 
        gagcagcac cagggcaac tactcccaa gtgatgccg 
        ttcctcttc aaccaaacc accgagcac gtgctggtc 
        ttccccacc tcggactcc atctacccg taccgggtg 
        aaggcctgg agcagcggc gccaagctg gcgagcccg 
        gtcctggtg gtggtgcgc caggagcgc gaggtgatc 
        tcctggcag gtgcccttc gtggtggac accaccatg 
        aaggaggga gtggtgcac tttcacaac acctcgcgg 
        acgctctgc cacaacgac atgcccagg attgccaaa 
        gccaaggcc acttccagg atcttgccc atccagctg 
        tcccagaat ttcatcatc gcactctcg acttcgtcg 
        ctggttaat gtggacatt tcggtgatg gtggaggag 
        gagcgggac ttttatttg caggaggga cggccgtat 
        gaggtgtcc gtgtcgccg agcgagtca aagtactac 
        tactacaag ttccatgat aagaagaac acaagtgcg 
        atgattgaa atcaactcg gatgatgat gtttgtttg 
        acggtttcc atccaggat agcttttgc ccggtcttg 
        gacttggat aaggatatt acgtacgag gggaagtac 
        cagacgatc aatcggaaa ggaggaatg acaatcaga 
        caacgcgaa ttccccgac ggcttcttc ctggtcttc 
        gtggcgaaa gccgacaac taccaatgc agccagaaa 
        cactcagtc ctcctcgtt gagcaccgc aaacagcac 
        ctgatcctc gccaatcgt acttccacc atcaccttc 
        accatcaac aagggcata aatggcaaa gagtacgag 
        atcgcctcc ttggccact cttggagcg ctcctatcc 
        ttctgcata gtgagcacc atcatgatt tttgccttc 
        acgagatgg ggcaccatc tccaagttc cggccgagc 
        ggcgacgag ctcgacgcc gactggaag gagcccccg 
        gagccccca atcacgcgc gaactcaag cacgagctg 
        ctgtcacgg caagcactg acggtcaac ctcctggcc 
        cgcgcccca gagaaggac aaacggcgc tcctacaac 
        tacctgtgg cacattctg agcatcgcg attttttac 
        agcatcccg gtggtgcaa ctcgtcatc acgtaccag 
        cgggtggtt aacaggact ggcgaccag gacatgtgc 
        tactataat tttttgtgt gccaatccg gcctttggc 
        ttgagtgat ttcaaccac attttctca aacgtgggg 
        tatatcata gttgggatt ttgttccta ggggtggta 
        ctccatcgg cagaccaaa atccccaac agttcgact 
        gggattccg gtccactat ggggtttat tacgccatg 
        gggattgcc ctgatcatt gagggcatt ttgtcggcc 
        tgctaccac atatgcccc agccagtcc aactatcaa 
        tttgatacc agctttatg tacgtgatg gcggtactt 
        tgcatgatt aaattgtac caaaatagg cacccagac 
        gtcaacgcc acggcctac gccaccttc acggtgctc 
        ggaatggcc atttttttg gcaatgatc gggatcctg 
        aacggcagt ctaacagtg tggattgtt tttgttgtg 
        atttattcg ctcttgtgt gcctatatt tccttcaaa 
        atctacttt atcagtttt gtgtttgat ggttttaaa 
        caactgaag caaagtctc aaaagtagt aataaagtt 
        gaagcgatt gctccgatt cggaaaagc cgttttgcc 
        cttcttgtg attgcgaat ataatcaat tatgcgatg 
        ttgattact gggctttgt ctctacaat accggggtt 
        accgatttt gggacgttt cttctaggg ttgctgatg 
        gggaattcg gtgctgtat gcggttttt tacacagga 
        atgaagcta gtcaatggc gaaaggatc tgttttgag 
        gccataatt tacggactt ttggcaatc gcagcttgg 
        gccacagcc actgtctac tttttagac aatgcaaca 
        ctttggact gtaactcct gccgaatcg cgccagtgg 
        aaccaagag tgcatcgtg atgagtttc tacgacaaa 
        cacgacgtt tggcactta ttaagcgcc cccgccctt 
        tacctaact ttcatgttt ttgttaagt ttggacgat 
        gatctcgtc gacacaaaa cgcgaggaa ataaccgtc 
        ttttag
      mRNA sequence
      • >TC011760 RA
        agcagataa cacaatctt tccgcaaac aagtgcaaa 
        tctatattt acttagaaa cctacaaaa cgtgccaag 
        actgtctca attattaac acccttcca ccccaagcg 
        aaagtaatt atccttgaa accgtgatc gatattcga 
        tcaatcaat tattaattc cttcctccc atcaataat 
        ggaaacaca aacgtgtct ctcagtcgc gtcccatgc 
        acctctcgg gtccaatgc cgaccttga accgcaccc 
        ttgcccaga taacactat cgatcctcc aggaagcgg 
        tgatttcgc tccgatgat aactcatta tcagttcag 
        ttcgagcct cgcgccatg atcgccgcc gccggcctg 
        ctcctgctg gtccccctc gcgggctgc gcccacatc 
        gcctccctc aacatcgag cagcaccag ggcaactac 
        tcccaagtg atgccgttc ctcttcaac caaaccacc 
        gagcacgtg ctggtcttc cccacctcg gactccatc 
        tacccgtac cgggtgaag gcctggagc agcggcgcc 
        aagctggcg agcccggtc ctggtggtg gtgcgccag 
        gagcgcgag gtgatctcc tggcaggtg cccttcgtg 
        gtggacacc accatgaag gagggagtg gtgcacttt 
        cacaacacc tcgcggacg ctctgccac aacgacatg 
        cccaggatt gccaaagcc aaggccact tccaggatc 
        ttgcccatc cagctgtcc cagaatttc atcatcgca 
        ctctcgact tcgtcgctg gttaatgtg gacatttcg 
        gtgatggtg gaggaggag cgggacttt tatttgcag 
        gagggacgg ccgtatgag gtgtccgtg tcgccgagc 
        gagtcaaag tactactac tacaagttc catgataag 
        aagaacaca agtgcgatg attgaaatc aactcggat 
        gatgatgtt tgtttgacg gtttccatc caggatagc 
        ttttgcccg gtcttggac ttggataag gatattacg 
        tacgagggg aagtaccag acgatcaat cggaaagga 
        ggaatgaca atcagacaa cgcgaattc cccgacggc 
        ttcttcctg gtcttcgtg gcgaaagcc gacaactac 
        caatgcagc cagaaacac tcagtcctc ctcgttgag 
        caccgcaaa cagcacctg atcctcgcc aatcgtact 
        tccaccatc accttcacc atcaacaag ggcataaat 
        ggcaaagag tacgagatc gcctccttg gccactctt 
        ggagcgctc ctatccttc tgcatagtg agcaccatc 
        atgattttt gccttcacg agatggggc accatctcc 
        aagttccgg ccgagcggc gacgagctc gacgccgac 
        tggaaggag cccccggag cccccaatc acgcgcgaa 
        ctcaagcac gagctgctg tcacggcaa gcactgacg 
        gtcaacctc ctggcccgc gccccagag aaggacaaa 
        cggcgctcc tacaactac ctgtggcac attctgagc 
        atcgcgatt ttttacagc atcccggtg gtgcaactc 
        gtcatcacg taccagcgg gtggttaac aggactggc 
        gaccaggac atgtgctac tataatttt ttgtgtgcc 
        aatccggcc tttggcttg agtgatttc aaccacatt 
        ttctcaaac gtggggtat atcatagtt gggattttg 
        ttcctaggg gtggtactc catcggcag accaaaatc 
        cccaacagt tcgactggg attccggtc cactatggg 
        gtttattac gccatgggg attgccctg atcattgag 
        ggcattttg tcggcctgc taccacata tgccccagc 
        cagtccaac tatcaattt gataccagc tttatgtac 
        gtgatggcg gtactttgc atgattaaa ttgtaccaa 
        aataggcac ccagacgtc aacgccacg gcctacgcc 
        accttcacg gtgctcgga atggccatt tttttggca 
        atgatcggg atcctgaac ggcagtcta acagtgtgg 
        attgttttt gttgtgatt tattcgctc ttgtgtgcc 
        tatatttcc ttcaaaatc tactttatc agttttgtg 
        tttgatggt tttaaacaa ctgaagcaa agtctcaaa 
        agtagtaat aaagttgaa gcgattgct ccgattcgg 
        aaaagccgt tttgccctt cttgtgatt gcgaatata 
        atcaattat gcgatgttg attactggg ctttgtctc 
        tacaatacc ggggttacc gattttggg acgtttctt 
        ctagggttg ctgatgggg aattcggtg ctgtatgcg 
        gttttttac acaggaatg aagctagtc aatggcgaa 
        aggatctgt tttgaggcc ataatttac ggacttttg 
        gcaatcgca gcttgggcc acagccact gtctacttt 
        ttagacaat gcaacactt tggactgta actcctgcc 
        gaatcgcgc cagtggaac caagagtgc atcgtgatg 
        agtttctac gacaaacac gacgtttgg cacttatta 
        agcgccccc gccctttac ctaactttc atgtttttg 
        ttaagtttg gacgatgat ctcgtcgac acaaaacgc 
        gaggaaata accgtcttt taggtgatt tatttgtga 
        taaccaatt tttatatcg ttgttgttt gaaataaat 
        gaataaaac gcaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY