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Gene information
General information
TC011774 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13703017..13707649
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011774
    iBeetle screen iB_06515

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011774 RA
      MKRERTGDN CRPFKLAHQ IVSLTGISF ERKSIIGFV 
      ELTVVPLRD NLRHVRLNA KQCRIYRII LNDAYEAHF 
      QYFDPFLDV CQSDTDTRS LETFSTHHL NAALKVDPD 
      NNSGELVVT IPPEASHLV AEGTALRVG IEFSLEQPQ 
      GGIHFVVPP KEGTYAERG VHLFTYGHQ NASRLWFPC 
      VDSFAETCT WKLEFTVDD EMTAISNGD LIEVVYTPD 
      MRRKTYHYS LNVPTCAPN ISLAVGPFE IYVDPYMHE 
      VTHFCLPNL LPLLKVTTR YMHETFEFY EETLSNRYP 
      YSCYKQVFV DETHEDYRS YATMSILSV NLLHSAVII 
      DQVYITRTI MAQAIAEQF FGCFISMQN WSDMWLNKG 
      ISQYLCGLY CKKSFGNNE YRYWVQSML QDVVKYEEQ 
      FGGIVLDPS QPPAPLPVG GGSNASQTN TKQEEKFYF 
      SIRNLHTMS PMYIKALEK KAFLVMRML EHRIGQELL 
      LQVFNKQLS LATNAAQQK IGSGLWGHM LISTNVFTK 
      AIFTVTGKD MAVFIDQWV RTGGHAKFH LTSVFNRKR 
      NTIELEIRQ DATTQLGIR KYVGPLLVT LQELDGTFK 
      HMLQIENTV VKADITCHS KSRRNKKKK IPLCTGDEV 
      DMDLSAMDD SPVLWIRLD PEMTLLRSV VIEQPDYQW 
      QYQLRHERD VTAQMEAIY ALERYPTPA TRLALTDTI 
      ENEQCYYKV RCRAAHCLT KVANAMVAN WAGPPAMLA 
      IFRKLFGSF AAPHIIRQN SFTNFQHYY LQKTIPLAM 
      AGLRNAHGI CPPDVIRFL LDLFKYNDN SKNRYSDNY 
      YRAALVEAL GNTVTPVVS VIHQGAPIT AESLSTDTK 
      LILEEVTRM LNLEKALPC YKYTVSVAC LKAIRKLQK 
      FGHLPSRPT IFKSYASYG HFIDVRVAA LECLVDFVR 
      ADGRWNDLQ DILDILESD PDPGLRHKL ADLIITNPP 
      FRRAHRERL DIPDLVERI WTNINGMLS HDTRLRCDL 
      VDFYYTLYG SKRPVCLPI PELAGMMNM HKHDARKTT 
      PYVPKPLPV PEMKHSPTP KDSTTPMII EDNLGCMNV 
      EVVASEDRI KEEPMDMHV EPRPTPKQE YFSDNSVSL 
      PGMGQSGPV GFEPGMFKK EDSKHKGDT GKIKKKKKD 
      KKKHKHKHK HKHEHKHGK DKKEKDPMK KMKEETLSS 
      VSSTPSPNP MSMGGETI
    CDS sequence
    • >TC011774 RA
      atgaagcga gaacgcacc ggggacaac tgccgcccc 
      tttaaatta gcccatcaa attgtgagc cttactggg 
      attagtttt gagcggaag agcataatt ggcttcgtt 
      gaattgacg gtggtgccc ctgcgagac aacctgaga 
      cacgtcaga ttgaacgcc aagcagtgc cgcatttac 
      cgcattatt ttaaacgac gcctatgag gcccatttt 
      cagtatttt gacccgttt ttggacgtc tgccagtcg 
      gacacagac acacgcagc ctcgagacc ttctccacg 
      caccacctg aatgccgcc ctcaaggtg gacccggac 
      aacaactcc ggcgagctg gtggtcacc atcccgccg 
      gaggcgtcg catctcgtg gccgagggc accgccctc 
      cgcgtcggc atcgaattc tcgctcgag cagccccaa 
      gggggcatc cacttcgtg gtgccgccc aaggagggg 
      acgtacgcc gaacgcggc gtccacttg ttcacctac 
      ggacaccag aacgcctcg cgactgtgg ttcccgtgc 
      gtggacagc ttcgccgag acctgcacc tggaagctg 
      gagttcacc gttgacgat gagatgacg gcgatctca 
      aacggcgat ttgatcgaa gtggtgtac actccggac 
      atgcgccgg aagacttat cactactcg ctaaatgtc 
      cccacctgc gcccccaat atttcgctg gccgtgggc 
      ccctttgag atctacgtg gacccctac atgcacgag 
      gtgacccac ttttgtctc cccaatctc cttccgttg 
      ttgaaagtg accacgcga tatatgcac gaaactttc 
      gagttttac gaagaaact ttatcaaat cgttatccg 
      tactcgtgt tataagcaa gtttttgtc gacgagaca 
      catgaggat taccggtct tacgcaaca atgagcatt 
      ttgagtgtc aatttgtta cactcggcg gtcattatt 
      gaccaggtt tacatcact aggaccatc atggcccag 
      gcgatagcc gagcagttc ttcggttgc ttcatttcg 
      atgcaaaat tggtcggac atgtggctc aacaagggc 
      ataagccag tacttgtgc ggtctctat tgcaagaaa 
      agcttcggt aacaacgag taccgatac tgggtgcag 
      tcgatgctc caggacgtt gtgaaatac gaggaacag 
      ttcgggggg attgtcttg gaccccagc cagcccccg 
      gcccccttg ccggtgggt gggggctcc aacgcctct 
      caaactaac accaaacaa gaggagaag ttttatttc 
      tcgatcagg aaccttcac acaatgtcc cccatgtac 
      atcaaagct ttggaaaag aaggcgttt cttgtgatg 
      cgtatgctg gagcaccgt attggccag gagttgttg 
      ttgcaagtc ttcaataaa caactctca ttggctacg 
      aatgcggcg caacagaaa atcgggagt ggattatgg 
      gggcacatg ctcatcagc actaatgtc ttcacgaag 
      gcgattttt acggtgacg gggaaagat atggctgtt 
      tttattgat cagtgggtg cggacgggg ggccatgcc 
      aagtttcac ttgacttcg gtttttaac cggaagaga 
      aatacaatc gaattggag ataagacag gatgccacg 
      acgcagttg gggattaga aaatatgtg gggcccctt 
      ttggtgacg ttgcaagag ttggacggg acttttaag 
      catatgttg caaattgag aatacggtg gtgaaggcc 
      gacatcacg tgccattcc aagagtcgg agaaataaa 
      aagaagaaa attccgctg tgtacaggg gacgaggtc 
      gatatggat ttgagtgcc atggatgat tcccccgtc 
      ctctggatc cgcctggac cccgaaatg acgctcctc 
      cgctcggtc gtaatcgag caacccgac taccaatgg 
      cagtaccag ctccgccac gaacgtgac gtcaccgcc 
      cagatggaa gccatctac gccctggag cgttacccc 
      acgcccgcc acccgtctg gccctcacc gacaccatc 
      gagaacgaa cagtgctat tacaaagtg cgctgtcgt 
      gccgcccac tgtctcacc aaggtggca aatgccatg 
      gtggccaat tgggccggc ccgcccgcc atgctggcg 
      atttttcgc aaattgttt ggctcgttt gccgctccg 
      catattata agacagaac agtttcacc aatttccaa 
      cactactac ttgcaaaag accatcccg ttggccatg 
      gcggggtta cggaacgcg cacgggatt tgtccgccg 
      gatgtgatt cgattcttg ctcgatttg ttcaaatat 
      aacgataat tcgaaaaat cgctactcg gataattac 
      tatcgagcg gctttggtc gaagctttg gggaatact 
      gtgactccg gtggtgtcg gtgatacac cagggggcg 
      ccaattacg gccgagagt ctcagtact gacactaaa 
      ctgatcctc gaagaggtc accaggatg ttgaacctt 
      gagaaagcc ctaccgtgt tacaaatat acggtttcg 
      gtggcgtgt cttaaggcc attcggaag ttgcaaaag 
      tttggccat ttgccaagc cgtcccacc attttcaag 
      agttacgcc agttatgga catttcatt gacgttcga 
      gttgcggcc ctcgaatgt ttggttgat tttgtgcga 
      gctgacggt cgctggaat gaccttcaa gacatactt 
      gatatttta gaaagtgat cctgacccc ggcctacgc 
      cacaaacta gccgacttg attataacc aatcctccg 
      ttcagaagg gcccatcgc gaacggttg gacatccct 
      gatttagtc gagcggatt tggacaaac atcaacggc 
      atgttgtca catgataca agactgagg tgcgatttg 
      gtcgatttt tactacacc ttgtacgga agtaaacgc 
      ccagtttgt ctcccgata ccggaattg gccggcatg 
      atgaacatg cataaacac gacgcgcgg aaaaccacg 
      ccttatgtc cctaagccg ctccccgtc cccgaaatg 
      aagcactcc cccaccccg aaagacagt actacgcca 
      atgataatc gaggataac ttgggctgt atgaatgtg 
      gaggtggtg gccagcgag gatcggatc aaggaggag 
      ccgatggat atgcacgtg gagccacgg ccgactccg 
      aaacaggag tatttttcg gataattcc gtttcgttg 
      cctggaatg ggtcagagt gggccggtt gggtttgag 
      cccgggatg ttcaagaag gaggattca aagcacaag 
      ggggatacg gggaagatt aagaagaag aagaaggat 
      aagaagaag cacaaacat aaacataag cataaacac 
      gagcataag cacggaaag gataagaag gagaaggat 
      ccgatgaag aagatgaaa gaggagacg ttgagttcg 
      gtcagctcg acgccgagt cccaacccc atgtccatg 
      gggggagag acgatttga
    mRNA sequence
    • >TC011774 RA
      ctgtcaaaa acaatctaa cctcagaaa actgttggt 
      atacaaatc ccatcgcaa tgtttaccc caaaatccc 
      tctaagctc gcaaaaacc acccaaaaa tgaagcgag 
      aacgcaccg gggacaact gccgcccct ttaaattag 
      cccatcaaa ttgtgagcc ttactggga ttagttttg 
      agcggaaga gcataattg gcttcgttg aattgacgg 
      tggtgcccc tgcgagaca acctgagac acgtcagat 
      tgaacgcca agcagtgcc gcatttacc gcattattt 
      taaacgacg cctatgagg cccattttc agtattttg 
      acccgtttt tggacgtct gccagtcgg acacagaca 
      cacgcagcc tcgagacct tctccacgc accacctga 
      atgccgccc tcaaggtgg acccggaca acaactccg 
      gcgagctgg tggtcacca tcccgccgg aggcgtcgc 
      atctcgtgg ccgagggca ccgccctcc gcgtcggca 
      tcgaattct cgctcgagc agccccaag ggggcatcc 
      acttcgtgg tgccgccca aggagggga cgtacgccg 
      aacgcggcg tccacttgt tcacctacg gacaccaga 
      acgcctcgc gactgtggt tcccgtgcg tggacagct 
      tcgccgaga cctgcacct ggaagctgg agttcaccg 
      ttgacgatg agatgacgg cgatctcaa acggcgatt 
      tgatcgaag tggtgtaca ctccggaca tgcgccgga 
      agacttatc actactcgc taaatgtcc ccacctgcg 
      cccccaata tttcgctgg ccgtgggcc cctttgaga 
      tctacgtgg acccctaca tgcacgagg tgacccact 
      tttgtctcc ccaatctcc ttccgttgt tgaaagtga 
      ccacgcgat atatgcacg aaactttcg agttttacg 
      aagaaactt tatcaaatc gttatccgt actcgtgtt 
      ataagcaag tttttgtcg acgagacac atgaggatt 
      accggtctt acgcaacaa tgagcattt tgagtgtca 
      atttgttac actcggcgg tcattattg accaggttt 
      acatcacta ggaccatca tggcccagg cgatagccg 
      agcagttct tcggttgct tcatttcga tgcaaaatt 
      ggtcggaca tgtggctca acaagggca taagccagt 
      acttgtgcg gtctctatt gcaagaaaa gcttcggta 
      acaacgagt accgatact gggtgcagt cgatgctcc 
      aggacgttg tgaaatacg aggaacagt tcgggggga 
      ttgtcttgg accccagcc agcccccgg cccccttgc 
      cggtgggtg ggggctcca acgcctctc aaactaaca 
      ccaaacaag aggagaagt tttatttct cgatcagga 
      accttcaca caatgtccc ccatgtaca tcaaagctt 
      tggaaaaga aggcgtttc ttgtgatgc gtatgctgg 
      agcaccgta ttggccagg agttgttgt tgcaagtct 
      tcaataaac aactctcat tggctacga atgcggcgc 
      aacagaaaa tcgggagtg gattatggg ggcacatgc 
      tcatcagca ctaatgtct tcacgaagg cgattttta 
      cggtgacgg ggaaagata tggctgttt ttattgatc 
      agtgggtgc ggacggggg gccatgcca agtttcact 
      tgacttcgg tttttaacc ggaagagaa atacaatcg 
      aattggaga taagacagg atgccacga cgcagttgg 
      ggattagaa aatatgtgg ggccccttt tggtgacgt 
      tgcaagagt tggacggga cttttaagc atatgttgc 
      aaattgaga atacggtgg tgaaggccg acatcacgt 
      gccattcca agagtcgga gaaataaaa agaagaaaa 
      ttccgctgt gtacagggg acgaggtcg atatggatt 
      tgagtgcca tggatgatt cccccgtcc tctggatcc 
      gcctggacc ccgaaatga cgctcctcc gctcggtcg 
      taatcgagc aacccgact accaatggc agtaccagc 
      tccgccacg aacgtgacg tcaccgccc agatggaag 
      ccatctacg ccctggagc gttacccca cgcccgcca 
      cccgtctgg ccctcaccg acaccatcg agaacgaac 
      agtgctatt acaaagtgc gctgtcgtg ccgcccact 
      gtctcacca aggtggcaa atgccatgg tggccaatt 
      gggccggcc cgcccgcca tgctggcga tttttcgca 
      aattgtttg gctcgtttg ccgctccgc atattataa 
      gacagaaca gtttcacca atttccaac actactact 
      tgcaaaaga ccatcccgt tggccatgg cggggttac 
      ggaacgcgc acgggattt gtccgccgg atgtgattc 
      gattcttgc tcgatttgt tcaaatata acgataatt 
      cgaaaaatc gctactcgg ataattact atcgagcgg 
      ctttggtcg aagctttgg ggaatactg tgactccgg 
      tggtgtcgg tgatacacc agggggcgc caattacgg 
      ccgagagtc tcagtactg acactaaac tgatcctcg 
      aagaggtca ccaggatgt tgaaccttg agaaagccc 
      taccgtgtt acaaatata cggtttcgg tggcgtgtc 
      ttaaggcca ttcggaagt tgcaaaagt ttggccatt 
      tgccaagcc gtcccacca ttttcaaga gttacgcca 
      gttatggac atttcattg acgttcgag ttgcggccc 
      tcgaatgtt tggttgatt ttgtgcgag ctgacggtc 
      gctggaatg accttcaag acatacttg atattttag 
      aaagtgatc ctgaccccg gcctacgcc acaaactag 
      ccgacttga ttataacca atcctccgt tcagaaggg 
      cccatcgcg aacggttgg acatccctg atttagtcg 
      agcggattt ggacaaaca tcaacggca tgttgtcac 
      atgatacaa gactgaggt gcgatttgg tcgattttt 
      actacacct tgtacggaa gtaaacgcc cagtttgtc 
      tcccgatac cggaattgg ccggcatga tgaacatgc 
      ataaacacg acgcgcgga aaaccacgc cttatgtcc 
      ctaagccgc tccccgtcc ccgaaatga agcactccc 
      ccaccccga aagacagta ctacgccaa tgataatcg 
      aggataact tgggctgta tgaatgtgg aggtggtgg 
      ccagcgagg atcggatca aggaggagc cgatggata 
      tgcacgtgg agccacggc cgactccga aacaggagt 
      atttttcgg ataattccg tttcgttgc ctggaatgg 
      gtcagagtg ggccggttg ggtttgagc ccgggatgt 
      tcaagaagg aggattcaa agcacaagg gggatacgg 
      ggaagatta agaagaaga agaaggata agaagaagc 
      acaaacata aacataagc ataaacacg agcataagc 
      acggaaagg ataagaagg agaaggatc cgatgaaga 
      agatgaaag aggagacgt tgagttcgg tcagctcga 
      cgccgagtc ccaacccca tgtccatgg ggggagaga 
      cgatttgat tttattgtg gcaattttt gcaaaaata 
      aagttattt aaggtgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY