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Gene information
General information
TC011785 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13502805..13522794
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011785
    iBeetle screen iB_06514

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    remarks: eggs lost,
    Adult morphology
    remarks: eggs lost,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: eggs lost,
    remarks - cuticles: later than schedule,
     
    8 days later, due to lost eggs in day 9
     

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011785 RA
      MSCSGDQVI FEMNARHRR FLQTLSLAA LLAYTALFA 
      YQTLSRGGV ADEENVLAE KRHQRAPLE EASVTSESS 
      SATATLRPP STVLDDVFI SVKTTKNYH RQRLPIILK 
      TWFQLAKAQ TWFFTDTDD PEFQHKTNG HMINTNCSS 
      SHNRKALCC KMSVEFDTF IDTDKKWFC HFDDDNYVN 
      VPRLVRFLG DYNPREDWY LGKPSIQAP LEIINKEKK 
      PTQKVKFWF ATGGAGFCL SRALALKMM PVASGGKFI 
      STGEKIRLP DDVTMGYII EHLLKKPLT VVDQFHSHL 
      EPMKFIRRE ILEEQISFS YSRNKDEWN VVKIEGFDT 
      KYDTNRFLS LHCFLFPHF NFCPR
    CDS sequence
    • >TC011785 RA
      atgtcgtgc tccggtgac caggtgatt ttcgaaatg 
      aacgccaga catcgacga tttttacag actctttcg 
      ttggccgct cttctcgca tacaccgcg ctcttcgcc 
      taccaaacg ctctcacgt ggaggagtc gccgacgaa 
      gagaacgtc ctcgccgag aaacgccac cagagagcc 
      cctctcgag gaggcctcc gtgaccagc gagagctcc 
      tcggcgacg gcgaccctc cggccgccc tccaccgtc 
      ctcgacgac gtcttcatc agcgtaaaa acgaccaaa 
      aactaccac cgccagcgc ctgcccatc atcctcaaa 
      acgtggttc cagttggcc aaagcccag acctggttt 
      ttcaccgac acggacgat ccggaattt cagcacaaa 
      acgaatggc catatgatc aacaccaac tgttcttcg 
      tcgcataac aggaaggca ctgtgctgc aagatgtca 
      gtcgagttt gacactttc attgatacc gacaaaaag 
      tggttctgc cacttcgac gacgacaac tacgtcaat 
      gtaccccga ctagtccgc ttcctgggg gattacaat 
      ccgagagaa gactggtat ttgggcaag cccagcatc 
      caagcaccg ttggagata atcaataag gagaaaaaa 
      ccgacgcaa aaagtcaag ttctggttc gccacgggc 
      ggcgcgggt ttctgctta agtcgggcg ttggccctc 
      aagatgatg ccggtagcc agtggcggc aagttcatc 
      agcaccggg gagaagatc cgtttgccg gacgacgtc 
      accatgggc tacataatc gagcacctg ctgaagaag 
      ccgttgacg gtcgtcgac cagttccac tcgcatctc 
      gagccgatg aagttcatc agacgggag atactcgaa 
      gagcagatc tcgttcagt tactcccgg aacaaagat 
      gaatggaac gtcgtcaag atcgaggga ttcgatacg 
      aaatacgac acgaacagg ttcctgtca ctgcactgc 
      ttcttgttc cctcatttc aacttctgt ccgcgatga
    mRNA sequence
    • >TC011785 RA
      catcagtcc tttaccatt gaaaggtaa ccatcgtgg 
      tcgtgcttg gcgtgtgcc ccaccacaa cttggaggg 
      acgaggggg accataaca aacaacaac cagcacaca 
      acatggtgc ccgagtcag tcacacatc gtctacttc 
      tcggagtcg ttagtctcc atagtggcc gttgttcac 
      gttcggcaa cacttgtgc ccttttcca ctcgaaaac 
      tatgttgtg gacttgttg ttcagcccc atgtcgtgc 
      tccggtgac caggtgatt ttcgaaatg aacgccaga 
      catcgacga tttttacag actctttcg ttggccgct 
      cttctcgca tacaccgcg ctcttcgcc taccaaacg 
      ctctcacgt ggaggagtc gccgacgaa gagaacgtc 
      ctcgccgag aaacgccac cagagagcc cctctcgag 
      gaggcctcc gtgaccagc gagagctcc tcggcgacg 
      gcgaccctc cggccgccc tccaccgtc ctcgacgac 
      gtcttcatc agcgtaaaa acgaccaaa aactaccac 
      cgccagcgc ctgcccatc atcctcaaa acgtggttc 
      cagttggcc aaagcccag acctggttt ttcaccgac 
      acggacgat ccggaattt cagcacaaa acgaatggc 
      catatgatc aacaccaac tgttcttcg tcgcataac 
      aggaaggca ctgtgctgc aagatgtca gtcgagttt 
      gacactttc attgatacc gacaaaaag tggttctgc 
      cacttcgac gacgacaac tacgtcaat gtaccccga 
      ctagtccgc ttcctgggg gattacaat ccgagagaa 
      gactggtat ttgggcaag cccagcatc caagcaccg 
      ttggagata atcaataag gagaaaaaa ccgacgcaa 
      aaagtcaag ttctggttc gccacgggc ggcgcgggt 
      ttctgctta agtcgggcg ttggccctc aagatgatg 
      ccggtagcc agtggcggc aagttcatc agcaccggg 
      gagaagatc cgtttgccg gacgacgtc accatgggc 
      tacataatc gagcacctg ctgaagaag ccgttgacg 
      gtcgtcgac cagttccac tcgcatctc gagccgatg 
      aagttcatc agacgggag atactcgaa gagcagatc 
      tcgttcagt tactcccgg aacaaagat gaatggaac 
      gtcgtcaag atcgaggga ttcgatacg aaatacgac 
      acgaacagg ttcctgtca ctgcactgc ttcttgttc 
      cctcatttc aacttctgt ccgcgatga gggggcgcc 
      ggagcacca ctaaactat ctgtgattt ttgtaaata 
      gagttggta taccttaat tctacgtcg gttttttgg 
      tgactagtc taacggatt ttaggcttc tagtttata 
      ttttaacaa ttcgttaac aaccaagaa ttaacatat 
      taacaattt tagctgatt gcaatctct tgtgatact 
      caaatcttg tgtagctat ttttatatt atctcacat 
      aggttttag gtgcaatta tattatatt tttgctcaa 
      agtgtaaat tgtacattt tttgtaaat actggatga 
      atgttgaac gtttttata tatttttaa aaataaaga 
      attacgtta tac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY