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Gene information
General information
TC012702 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13444306..13447105
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012702
    iBeetle screen iB_06513

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012702 RA
      MTSYTDKGP KPTNGKFLC FDHLTFYVG NAKQAASYY 
      ITRLGFEPL AYKGLESGS RKYAAHVVR QNKVIFVFM 
      SPYEPGDSE VGPHLVKHG DGVKDIAMA VENLEGIVA 
      RAKQKGAKI LKDIWEESD FHGIVRFAK IQTYGDTTH 
      TFIDRSLYR GPFLPGYVM CQEDKLAKN LPVARLDFI 
      DHIVGNQPD GEMESVAKW YETVLQFHR FWSVDDKQV 
      HTEYSALRS IVMSNWEEN VKLPINEPA PGKKKSQIE 
      EYVEYYGGA GVQHIALNT QDIISSVQN LMARGVEFL 
      QVPDSYYDI LREQLKHSK VKIAEDLDI LQKLKILID 
      YDDKGYLLQ IFTKNMQDR PTLFLEVIQ RRNHNGFGA 
      GNFKALFEA IEIEQAKRG NL
    CDS sequence
    • >TC012702 RA
      atgacttca tacacagac aagggcccc aagccaact 
      aacggcaaa tttttgtgc ttcgaccat ttaacgttt 
      tacgtcgga aatgcgaaa caagccgct agttattac 
      ataacaaga ctgggcttc gagcctttg gcttacaaa 
      ggcctggag agcggaagc cgcaaatat gccgcccat 
      gtcgtccgc cagaacaaa gtcattttt gtgtttatg 
      tccccttac gaacccggg gactccgag gtggggccc 
      cacctggtc aagcatggg gacggcgtt aaggacatc 
      gcgatggct gtagaaaat ttggaggga atcgttgcg 
      cgagccaaa caaaaaggt gccaaaata ctgaaagac 
      atctgggag gagagcgac tttcacgga atcgtccgc 
      ttcgccaaa atccaaacg tacggcgac actacccat 
      actttcatc gaccgcagc ctctaccgg ggccccttc 
      ttgcccggc tacgtcatg tgtcaagag gacaagttg 
      gcgaaaaat ttgcccgta gccaggctg gacttcatc 
      gaccacatc gtgggcaac cagcccgac ggggagatg 
      gagtccgtg gcgaaatgg tacgagacg gtcctgcaa 
      ttccaccgc ttctggtcg gtggacgac aagcaagtg 
      cacaccgaa tattcggcg ttacggtcc attgtgatg 
      agcaactgg gaggagaat gtgaagttg ccgatcaac 
      gagcccgcc cctggcaag aagaagagc cagatcgag 
      gagtacgtg gagtactat gggggcgcg ggggtgcag 
      cacatcgcc ctcaacacg caggacatc atcagctcg 
      gtgcagaat ttgatggct agaggggtt gagtttctc 
      caagtgccg gacagttat tacgacatt ctgcgcgag 
      cagctgaag cacagtaag gtgaagatt gccgaggat 
      ttggatatt ttgcaaaaa ttgaagatt ctgattgat 
      tatgacgac aaggggtat ttgctgcaa atcttcacc 
      aagaatatg caagaccgg ccgactctc ttcctggag 
      gttattcaa agaagaaac cataacgga tttggggcc 
      gggaatttc aaggcacta ttcgaggcg attgaaata 
      gaacaagcg aaacgtggc aacttgtaa
    mRNA sequence
    • >TC012702 RA
      cagtcaagt ttcagtgag tgaaaatga cttcataca 
      cagacaagg gccccaagc caactaacg gcaaatttt 
      tgtgcttcg accatttaa cgttttacg tcggaaatg 
      cgaaacaag ccgctagtt attacataa caagactgg 
      gcttcgagc ctttggctt acaaaggcc tggagagcg 
      gaagccgca aatatgccg cccatgtcg tccgccaga 
      acaaagtca tttttgtgt ttatgtccc cttacgaac 
      ccggggact ccgaggtgg ggccccacc tggtcaagc 
      atggggacg gcgttaagg acatcgcga tggctgtag 
      aaaatttgg agggaatcg ttgcgcgag ccaaacaaa 
      aaggtgcca aaatactga aagacatct gggaggaga 
      gcgactttc acggaatcg tccgcttcg ccaaaatcc 
      aaacgtacg gcgacacta cccatactt tcatcgacc 
      gcagcctct accggggcc ccttcttgc ccggctacg 
      tcatgtgtc aagaggaca agttggcga aaaatttgc 
      ccgtagcca ggctggact tcatcgacc acatcgtgg 
      gcaaccagc ccgacgggg agatggagt ccgtggcga 
      aatggtacg agacggtcc tgcaattcc accgcttct 
      ggtcggtgg acgacaagc aagtgcaca ccgaatatt 
      cggcgttac ggtccattg tgatgagca actgggagg 
      agaatgtga agttgccga tcaacgagc ccgcccctg 
      gcaagaaga agagccaga tcgaggagt acgtggagt 
      actatgggg gcgcggggg tgcagcaca tcgccctca 
      acacgcagg acatcatca gctcggtgc agaatttga 
      tggctagag gggttgagt ttctccaag tgccggaca 
      gttattacg acattctgc gcgagcagc tgaagcaca 
      gtaaggtga agattgccg aggatttgg atattttgc 
      aaaaattga agattctga ttgattatg acgacaagg 
      ggtatttgc tgcaaatct tcaccaaga atatgcaag 
      accggccga ctctcttcc tggaggtta ttcaaagaa 
      gaaaccata acggatttg gggccggga atttcaagg 
      cactattcg aggcgattg aaatagaac aagcgaaac 
      gtggcaact tgtaatagt ttaatgtac ctaataaat 
      aataatcat tagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY