| General information | |||
| TC034437 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 13411855..13431775 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034437 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06511 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034437 RA
MSNFAQLAV RCFLLCSAL NLKNVAEGW KGDPRIHNC FWNWKIMER NPFPTDYAV HLSPRWNDY QERLNFPDM PFINYSYNW EMEQIDGNT VHKVDVSEW IDLLHNARM IIPAFALRP GKYQIYPNI KPLTPDVPK AIIKFCPIL YYSKKPTIL FAGGAHQIV PPQSELIVS AESSFDTNL RKRSYKDLA FEWSCKQKK SSFCKHLSV STDNRLVIP AAHVLDGDV IQVSVKATT KDSGKGDII SSITSIKII EQVKEDDYL WTIERKDKK PLDFDYEKS TQFGRNVSK FVIEASALR KGDYLVTVR LRESHPLKG YSQGELSFA GMPRISNCQ ISPPKGLTH HFFKVSCIQ TNIYENNVY ELYAVKKPN TGKLADADL VVSGTYPLV NHEEFQLPD SDTYEYILK IKSLGGATQ QFKLKATIT SEMKKYNDY NFKMALTYR YAKLGHNEV RKNILAENA DKRKRGLHV LKAVIDDIV DRKFKSPGL KEFLTKLKF NVVEDMLAA PLDDLDQVL QMSNAVSKL VSDDAAPES DPLFSKFTT TFCKKMMDK HLEMVQNND YPLDLVDKV SRMSSSITT CLETNTNPN YKAIKPLDD INITTEAAV IDEPILTEN YENYVDFGD DYYKNLDYL KESTYNIIN ASAQAAKTM AFVAVAGEK FSETEGLKS KTVAAKDFG YELVKVQVK SHGVTVVPS PDFKDDKHS YDILLTSWW KDIMWWNPA KTRVSTEIA SVQFWHSSC KQRVTKFEK PADIYFQVK NGTRFKPKL LEDTFVVPA GYHGLTGEP LDRSVKVRR IALPPFQAI DVQFAVSDV FDVVFTKFE FPTSEHFAN ATTIKPGGA TSLRFVNDR KYAIWGYVG VLASKKVGS EGSLAYNSS YVVATCLRW IDKNNSWVF ACRSGKHGN SSVVHCECN EFSVLAGYL RDYRSDLQK VEDAELELV LKSTLTIFI TVVVTFGLY LFLMLFALI GKKYQVYFL PDNDRKSRF AYLVVIKTG IGRYASTSS NVTVRLVGT KMASMPHVL NYPDPKMRI LQRNNREVF VLATSAHLG DITKIEIWF DCTGPNPDW YCRFIIVYD VQTTQQWYF NVRKRLAVT KGESYISLT PTPSGQEAP KRKYVPKFR IANHSWNLC QSELNFGYA KKLTAIFSS VFMTYLSAL LLHGTPSLA LKDGLDNCE FEVDSFTVL VGLLSALVA FTCHIAIAY CFRFSKVQF SRDKDYAKL SFHVTIICW LLLIVVMSV SISALVIMG FWVPPNHSL RWLTSSGIG VAAGAIVYE TLFLFFLSI LWNLRLEKG DIIEWGFKD IWKNVEYQR GFLHRKFGD FILRPYFLH LYQPLDPKR ITQKQVVVE RRRNVISEL EDLVMFGIY VVILYVVVW SNKDRLIIG SKETMQDLM AGNHTRTLA FRNAGTPLA FYDFLNETL VPVIQPTRW YGNYVVAEP GLMLDMNNK IIGIARLRQ HRLKKELCD PPEILAFLN LTCEPEITW WRQEHRPYG YGWGNFSSY FVFDRLQQI WRYSGAINT GTFPSIGEI SSYTGGGYV GYLGRTLYN TYANFRRFF QKVWIDHGS RCIFIEFLT YNTNYNVFN AIRLMYEHS ASGYSVNTV EVNAVRMLF VNRELESIT VIVFFMFVA WVGVLFFKQ SVGVIRKGK SFYKDVWFM VDCVIILMS VACTVLFVV RMKMIGRYL DHLDKLKHN EFSNYFFLF YLEDFLQVF AASLVCIAT VRLWKFLRF GLFFRMMEC TIWKSLGPL LSFSVAFIC LLSAFALPL VLLRGSEIP ILNRIITVV RPMFIMALS AGEFPLKEF IRNRSSAFY IVLYGLIVK IVFLIYIMI IMMAYSTVQ MEFSNELEV YTIKNYVQE RFNYFPKYL KYKYKRLRS GQTAHRQVE PKPDKFLYD NSFSVVTSR MMTMKYIVR CVFRNMVRR ESAGLADGD VRLMLGVSR RFLVKAPPK EVEVFFKGR VSGKKISLI DERKVGKIA SVVSALLAQ RGPEEAQHF ARKLLVGEQ
- >TC034437 RA
atgtcgaat tttgcgcaa ctcgcagta aggtgtttc cttctgtgt tcggcgcta aatttaaaa aatgttgct gaagggtgg aaaggagac ccccgaatt cataactgt ttctggaac tggaaaatc atggagcgc aatcctttt ccaacagat tacgcggtt caccttagt cccagatgg aacgattat caggaacgt ttaaatttt cctgacatg ccttttata aattacagt tataattgg gagatggaa caaattgac ggcaatacc gtgcacaaa gttgacgtt tctgaatgg atagactta ttacacaat gccaggatg atcattccg gcttttgcg ctccggccc ggaaaatac caaatctac cccaacatc aaaccccta acccccgat gttccgaag gcaataata aaattttgc cctatcttg tattattcg aaaaagccg acgattctg ttcgcgggc ggcgcccac cagatagtg cctccccaa agtgagctc atcgtcagc gctgaatcc tcctttgac acgaatctg cgcaagcgg agctacaaa gacctcgct ttcgagtgg agttgcaag cagaaaaaa tcgtcattt tgcaaacat ttgtccgtt tcgaccgac aaccgcctg gtgatcccc gccgcccac gtgctggac ggagatgtg atccaggtc tccgtgaag gccacaaca aaagactcc ggcaaaggc gacatcatc agctccatc acctccata aaaattatc gaacaggtc aaagaggac gactacctg tggaccatc gagaggaag gacaaaaag ccgctggat tttgactac gaaaagagc acgcaattc gggcgaaac gtgtccaag tttgtgatc gaggcgagc gccctcaga aagggcgac tacctggtg acggtgcgg ctcagagag tcgcaccct ttgaagggc tactcgcag ggcgagctc agcttcgcg ggcatgccc cgcatcagc aactgccag atctcgccc cccaagggc ctcacgcac cacttcttc aaggtgtcc tgcatccag accaacatc tacgagaac aacgtctac gagctgtac gccgtgaaa aagcccaac acggggaag ctggccgac gccgatttg gtggtttcg gggacgtat ccgctagtt aaccacgag gagttccag ctgcccgac tcggacacc tacgagtat attttgaaa attaagagt ttgggaggg gcaacgcag cagtttaag ctcaaagcc accatcacg tccgaaatg aagaaatac aacgattac aattttaaa atggcactc acgtatcgc tatgcgaaa cttggccac aatgaagtt cgcaagaat attttggcc gagaacgcc gacaagaga aaacgaggg ctgcatgtg ctgaaggcg gtgattgac gatatcgtc gatagaaaa tttaaaagt ccaggtctt aaggagttt ctcacaaag ttgaagttt aatgtggtg gaggatatg ttggcggcg ccgctggac gacctcgat caagtgctt caaatgagc aatgcagtt tcaaagctg gtaagtgac gacgctgcg cccgaaagt gatccactg ttctcgaaa ttcacgaca acattttgc aaaaaaatg atggacaaa cacctggag atggtccag aacaatgac tatcctctc gatctcgtc gataaagtc agcagaatg tcaagttct ataacgact tgtttggaa acgaacaca aatccaaat tacaaggcg atcaagccc ttggacgat ataaacata accacggag gctgcagtc atcgacgag ccaatcctc acggaaaac tatgaaaat tacgtcgac ttcggtgac gattattac aagaacttg gattacttg aaagaatca acctacaat attatcaat gcgtcggct caggcggcc aagaccatg gcgtttgtg gctgtggcc ggcgaaaaa ttcagcgaa accgaaggc ttgaaaagt aagacagtc gcggcgaag gattttggt tacgaattg gttaaagtt caagtgaaa tcgcacgga gttacagtc gttccgtct ccagacttc aaggacgac aaacattct tatgacatt ttgctcacc agctggtgg aaggacatc atgtggtgg aacccggcg aagaccaga gtctccact gagatcgcc agcgtgcaa ttctggcac tcctcgtgc aagcaacgc gtgacaaag ttcgaaaaa ccggccgac atctacttc caagtgaaa aacggtacc agatttaag ccgaaactg ctggaggac acgtttgtg gtaccggct gggtaccac ggcctgact ggtgagcct ttggaccgc agtgtgaag gtccgaagg attgcccta ccgcccttt caagcaata gacgtccag tttgctgtt tcggacgtt ttcgatgtc gtgtttacc aaatttgag ttcccgact tctgaacac tttgctaat gccaccaca atcaagcct ggaggcgcc acaagtttg agatttgtc aatgaccga aagtacgcc atttggggc tatgtgggg gttctagcc agcaagaaa gtgggctct gagggaagc ctcgcttat aatagtagt tacgtggtg gcaacgtgc cttcgatgg atcgataag aacaattcg tgggttttt gcctgtagg tcagggaaa cacggtaat agcagtgtt gttcattgc gaatgcaac gagttttcc gtactcgcg gggtacttg cgagattac cgctccgac ttgcaaaaa gtcgaagac gcggaactg gaactggtg cttaaaagt acccttact atttttata actgtcgta gttacattt gggctttat ttatttctc atgttattt gcattgatc gggaagaag taccaagtg tattttttg cccgacaat gaccgaaaa agccggttt gcgtatctt gttgttatt aaaactggg attggacga tatgcttct acctcttcc aacgtgacc gttcgtcta gttggtacc aaaatggcc agcatgccc catgttttg aactaccct gaccccaaa atgcgcatc ttgcaacga aacaaccgc gaagtgttt gtgttagcc acaagcgcc catttgggc gacatcacc aaaatcgag atttggttc gactgcact ggaccgaac cctgactgg tactgccgt ttcatcata gtttacgac gtccaaacg acacagcag tggtacttt aatgtgaga aagcggctg gctgtcacg aagggcgaa agctacatc tcgttaacc cccacaccg agcggacag gaagctcca aagaggaaa tacgtaccg aagtttcgg atcgccaac cacagctgg aacctgtgc caaagcgag ctgaacttc ggatacgcg aaaaaacta actgctatt ttctcctcg gtgtttatg acctacctc agcgccctt ctcctccac ggaacacca agtcttgcc ctaaaggac ggtctcgac aattgcgag tttgaagtc gattccttc acagtcctg gtgggtttg ttgagcgcg cttgtggcc tttacgtgc catattgcg atcgcgtac tgcttcagg ttttcaaaa gtgcagttt tcgcgagat aaagactac gccaagtta tcgtttcat gtaactata atttgttgg ttgctcctc attgttgtg atgagtgtc tccatttct gcattggtg attatgggc ttttgggtg ccacccaat cacagcttg cgatggttg acttccagt ggtatcggt gttgctgct ggtgccatt gtttacgaa acacttttc ttgttcttc ctcagtatt ttgtggaat ttgaggctt gaaaaggga gatataatc gagtggggt ttcaaagac atttggaaa aatgttgag taccagagg gggttcctg catcggaaa tttggtgat tttattttg aggccgtac ttcctgcac ttgtaccaa ccactggac cctaaacga attactcaa aaacaggtg gttgtggag cgccgccgc aacgtcata tccgaatta gaagacttg gtcatgttt ggaatctac gttgtgatt ttgtacgtt gtggtgtgg tcgaacaag gaccgactc atcatcggt agcaaggaa acgatgcag gacctaatg gccggcaat cacacccgc actttggca tttaggaac gctggaacc cctttagct ttttacgac tttctgaac gaaacttta gtaccagtg atacaacca acacggtgg tatggcaac tacgtggtc gctgagccg ggcttaatg cttgacatg aacaacaaa attattgga attgcccgt ttgcgccaa catcgactc aaaaaggaa ctctgtgat ccacccgaa attttggct tttttgaat ttgacgtgc gagccggaa attacgtgg tggcgccag gagcacagg ccatatggc tatggttgg ggcaatttc agcagttat ttcgtattc gatcggctg cagcagatt tggcgctat tcgggggct atcaatact ggcaccttt ccatcaatt ggtgaaata tcgtcgtac acgggcggc ggctacgtg ggctacctg ggccgcacc ctgtacaac acctacgcc aacttccgg cgctttttc caaaaagtc tggatcgac cacggcagt cgttgcatt ttcatcgaa tttctcact tacaacacc aactacaac gtcttcaac gccattaga ttgatgtac gagcacagt gccagcggc tacagtgtg aacacagtc gaagtgaac gccgttcgc atgctcttc gtcaatagg gagctggag agcatcacc gtgatcgtg ttcttcatg ttcgtcgcc tgggttggg gtgcttttc ttcaagcag tcggtcggc gtgattcgc aaagggaaa agcttttac aaagacgtg tggtttatg gtcgactgc gtcataata ctgatgagt gtggcttgc actgtgctg ttcgtggtg cggatgaag atgatcggg cggtatctc gatcacctg gacaagctg aagcacaac gagttctcc aattacttt ttcctgttt tatttggag gactttttg caagtgttc gccgccagt ttggtgtgc atcgccacc gtgcggttg tggaagttc ctccggttt gggctcttc ttccgcatg atggagtgc accatctgg aagagtctg ggaccgctg ctcagtttc tcggtcgct ttcatttgc ctgctgtcc gcctttgcc ctgcctctg gtgctgctc cgcggctcc gaaatcccc atactgaac agaataatc acggtggtg cgcccgatg ttcatcatg gcgctgagt gccggggag tttccgttg aaggagttc attcggaac agaagttcg gcgttttac atcgtcttg tacgggctt atcgtgaag atcgtcttc ctgatctat atcatgatc atcatgatg gcctactcc acggtccag atggagttt tccaacgaa cttgaagtt tacactatc aagaactac gtccaagag cgcttcaac tacttcccc aagtacctc aagtacaag tacaagagg ctgaggtcg ggccaaacc gcgcacagg caagtggag ccaaagccg gacaagttc ctgtacgac aacagtttc tcggtggtg acgtccagg atgatgacg atgaagtac atcgtccgg tgcgttttt cgcaacatg gtgaggcgg gagagcgcg ggactggcc gacggggac gtgagactg atgctggga gtgagtcgg cggtttttg gtgaaggcg ccgcccaaa gaagtggag gtgttcttc aaagggagg gtttcgggg aagaagatt tcgctgatt gacgaacgc aaagtgggg aagattgcg agtgtggtg agcgcgttg ctggcacag agaggcccc gaggaggca cagcatttt gctcgaaaa ctattagtc ggagaacaa taa
- >TC034437 RA
taatggtaa agaatgtag aaaaatgta gaagagtct tgaaactaa ttgtagaat ttatgaaaa aagttggct gtgctcacg tcaaaaatg tcgaatttt gcgcaactc gcagtaagg tgtttcctt ctgtgttcg gcgctaaat ttaaaaaat gttgctgaa gggtggaaa ggagacccc cgaattcat aactgtttc tggaactgg aaaatcatg gagcgcaat ccttttcca acagattac gcggttcac cttagtccc agatggaac gattatcag gaacgttta aattttcct gacatgcct tttataaat tacagttat aattgggag atggaacaa attgacggc aataccgtg cacaaagtt gacgtttct gaatggata gacttatta cacaatgcc aggatgatc attccggct tttgcgctc cggcccgga aaataccaa atctacccc aacatcaaa cccctaacc cccgatgtt ccgaaggca ataataaaa ttttgccct atcttgtat tattcgaaa aagccgacg attctgttc gcgggcggc gcccaccag atagtgcct ccccaaagt gagctcatc gtcagcgct gaatcctcc tttgacacg aatctgcgc aagcggagc tacaaagac ctcgctttc gagtggagt tgcaagcag aaaaaatcg tcattttgc aaacatttg tccgtttcg accgacaac cgcctggtg atccccgcc gcccacgtg ctggacgga gatgtgatc caggtctcc gtgaaggcc acaacaaaa gactccggc aaaggcgac atcatcagc tccatcacc tccataaaa attatcgaa caggtcaaa gaggacgac tacctgtgg accatcgag aggaaggac aaaaagccg ctggatttt gactacgaa aagagcacg caattcggg cgaaacgtg tccaagttt gtgatcgag gcgagcgcc ctcagaaag ggcgactac ctggtgacg gtgcggctc agagagtcg caccctttg aagggctac tcgcagggc gagctcagc ttcgcgggc atgccccgc atcagcaac tgccagatc tcgcccccc aagggcctc acgcaccac ttcttcaag gtgtcctgc atccagacc aacatctac gagaacaac gtctacgag ctgtacgcc gtgaaaaag cccaacacg gggaagctg gccgacgcc gatttggtg gtttcgggg acgtatccg ctagttaac cacgaggag ttccagctg cccgactcg gacacctac gagtatatt ttgaaaatt aagagtttg ggaggggca acgcagcag tttaagctc aaagccacc atcacgtcc gaaatgaag aaatacaac gattacaat tttaaaatg gcactcacg tatcgctat gcgaaactt ggccacaat gaagttcgc aagaatatt ttggccgag aacgccgac aagagaaaa cgagggctg catgtgctg aaggcggtg attgacgat atcgtcgat agaaaattt aaaagtcca ggtcttaag gagtttctc acaaagttg aagtttaat gtggtggag gatatgttg gcggcgccg ctggacgac ctcgatcaa gtgcttcaa atgagcaat gcagtttca aagctggta agtgacgac gctgcgccc gaaagtgat ccactgttc tcgaaattc acgacaaca ttttgcaaa aaaatgatg gacaaacac ctggagatg gtccagaac aatgactat cctctcgat ctcgtcgat aaagtcagc agaatgtca agttctata acgacttgt ttggaaacg aacacaaat ccaaattac aaggcgatc aagcccttg gacgatata aacataacc acggaggct gcagtcatc gacgagcca atcctcacg gaaaactat gaaaattac gtcgacttc ggtgacgat tattacaag aacttggat tacttgaaa gaatcaacc tacaatatt atcaatgcg tcggctcag gcggccaag accatggcg tttgtggct gtggccggc gaaaaattc agcgaaacc gaaggcttg aaaagtaag acagtcgcg gcgaaggat tttggttac gaattggtt aaagttcaa gtgaaatcg cacggagtt acagtcgtt ccgtctcca gacttcaag gacgacaaa cattcttat gacattttg ctcaccagc tggtggaag gacatcatg tggtggaac ccggcgaag accagagtc tccactgag atcgccagc gtgcaattc tggcactcc tcgtgcaag caacgcgtg acaaagttc gaaaaaccg gccgacatc tacttccaa gtgaaaaac ggtaccaga tttaagccg aaactgctg gaggacacg tttgtggta ccggctggg taccacggc ctgactggt gagcctttg gaccgcagt gtgaaggtc cgaaggatt gccctaccg ccctttcaa gcaatagac gtccagttt gctgtttcg gacgttttc gatgtcgtg tttaccaaa tttgagttc ccgacttct gaacacttt gctaatgcc accacaatc aagcctgga ggcgccaca agtttgaga tttgtcaat gaccgaaag tacgccatt tggggctat gtgggggtt ctagccagc aagaaagtg ggctctgag ggaagcctc gcttataat agtagttac gtggtggca acgtgcctt cgatggatc gataagaac aattcgtgg gtttttgcc tgtaggtca gggaaacac ggtaatagc agtgttgtt cattgcgaa tgcaacgag ttttccgta ctcgcgggg tacttgcga gattaccgc tccgacttg caaaaagtc gaagacgcg gaactggaa ctggtgctt aaaagtacc cttactatt tttataact gtcgtagtt acatttggg ctttattta tttctcatg ttatttgca ttgatcggg aagaagtac caagtgtat tttttgccc gacaatgac cgaaaaagc cggtttgcg tatcttgtt gttattaaa actgggatt ggacgatat gcttctacc tcttccaac gtgaccgtt cgtctagtt ggtaccaaa atggccagc atgccccat gttttgaac taccctgac cccaaaatg cgcatcttg caacgaaac aaccgcgaa gtgtttgtg ttagccaca agcgcccat ttgggcgac atcaccaaa atcgagatt tggttcgac tgcactgga ccgaaccct gactggtac tgccgtttc atcatagtt tacgacgtc caaacgaca cagcagtgg tactttaat gtgagaaag cggctggct gtcacgaag ggcgaaagc tacatctcg ttaaccccc acaccgagc ggacaggaa gctccaaag aggaaatac gtaccgaag tttcggatc gccaaccac agctggaac ctgtgccaa agcgagctg aacttcgga tacgcgaaa aaactaact gctattttc tcctcggtg tttatgacc tacctcagc gcccttctc ctccacgga acaccaagt cttgcccta aaggacggt ctcgacaat tgcgagttt gaagtcgat tccttcaca gtcctggtg ggtttgttg agcgcgctt gtggccttt acgtgccat attgcgatc gcgtactgc ttcaggttt tcaaaagtg cagttttcg cgagataaa gactacgcc aagttatcg tttcatgta actataatt tgttggttg ctcctcatt gttgtgatg agtgtctcc atttctgca ttggtgatt atgggcttt tgggtgcca cccaatcac agcttgcga tggttgact tccagtggt atcggtgtt gctgctggt gccattgtt tacgaaaca cttttcttg ttcttcctc agtattttg tggaatttg aggcttgaa aagggagat ataatcgag tggggtttc aaagacatt tggaaaaat gttgagtac cagaggggg ttcctgcat cggaaattt ggtgatttt attttgagg ccgtacttc ctgcacttg taccaacca ctggaccct aaacgaatt actcaaaaa caggtggtt gtggagcgc cgccgcaac gtcatatcc gaattagaa gacttggtc atgtttgga atctacgtt gtgattttg tacgttgtg gtgtggtcg aacaaggac cgactcatc atcggtagc aaggaaacg atgcaggac ctaatggcc ggcaatcac acccgcact ttggcattt aggaacgct ggaacccct ttagctttt tacgacttt ctgaacgaa actttagta ccagtgata caaccaaca cggtggtat ggcaactac gtggtcgct gagccgggc ttaatgctt gacatgaac aacaaaatt attggaatt gcccgtttg cgccaacat cgactcaaa aaggaactc tgtgatcca cccgaaatt ttggctttt ttgaatttg acgtgcgag ccggaaatt acgtggtgg cgccaggag cacaggcca tatggctat ggttggggc aatttcagc agttatttc gtattcgat cggctgcag cagatttgg cgctattcg ggggctatc aatactggc acctttcca tcaattggt gaaatatcg tcgtacacg ggcggcggc tacgtgggc tacctgggc cgcaccctg tacaacacc tacgccaac ttccggcgc tttttccaa aaagtctgg atcgaccac ggcagtcgt tgcattttc atcgaattt ctcacttac aacaccaac tacaacgtc ttcaacgcc attagattg atgtacgag cacagtgcc agcggctac agtgtgaac acagtcgaa gtgaacgcc gttcgcatg ctcttcgtc aatagggag ctggagagc atcaccgtg atcgtgttc ttcatgttc gtcgcctgg gttggggtg cttttcttc aagcagtcg gtcggcgtg attcgcaaa gggaaaagc ttttacaaa gacgtgtgg tttatggtc gactgcgtc ataatactg atgagtgtg gcttgcact gtgctgttc gtggtgcgg atgaagatg atcgggcgg tatctcgat cacctggac aagctgaag cacaacgag ttctccaat tactttttc ctgttttat ttggaggac tttttgcaa gtgttcgcc gccagtttg gtgtgcatc gccaccgtg cggttgtgg aagttcctc cggtttggg ctcttcttc cgcatgatg gagtgcacc atctggaag agtctggga ccgctgctc agtttctcg gtcgctttc atttgcctg ctgtccgcc tttgccctg cctctggtg ctgctccgc ggctccgaa atccccata ctgaacaga ataatcacg gtggtgcgc ccgatgttc atcatggcg ctgagtgcc ggggagttt ccgttgaag gagttcatt cggaacaga agttcggcg ttttacatc gtcttgtac gggcttatc gtgaagatc gtcttcctg atctatatc atgatcatc atgatggcc tactccacg gtccagatg gagttttcc aacgaactt gaagtttac actatcaag aactacgtc caagagcgc ttcaactac ttccccaag tacctcaag tacaagtac aagaggctg aggtcgggc caaaccgcg cacaggcaa gtggagcca aagccggac aagttcctg tacgacaac agtttctcg gtggtgacg tccaggatg atgacgatg aagtacatc gtccggtgc gtttttcgc aacatggtg aggcgggag agcgcggga ctggccgac ggggacgtg agactgatg ctgggagtg agtcggcgg tttttggtg aaggcgccg cccaaagaa gtggaggtg ttcttcaaa gggagggtt tcggggaag aagatttcg ctgattgac gaacgcaaa gtggggaag attgcgagt gtggtgagc gcgttgctg gcacagaga ggccccgag gaggcacag cattttgct cgaaaacta ttagtcgga gaacaataa tcttttttg aaaaaaata gataattta aagagcttt ccaacgata atacacttc atggggtta t
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