Status
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Gene information
General information
TC011807 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13325436..13329063
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011807
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06510

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011807 RA
      MLDLLPFLS TILPIIASS LVICVFVVN LYLRIRRKF 
      PISVNCWFC NSWTKVPYD DRDGFDCPT CSQYNGFTS 
      DGGYNKIIP EQHDEFMNK STRVKSRKS GASNGLCAL 
      CNNNQQLKV FQLAHFVPM NEENYDVEI EHFQEQLEK 
      AYKLCKKCD KVLKKTISK QNAWIFGNR VRDLCHNGI 
      SKIASQIRK ETKLTLVRK LDGCLSILC VLAVIFTTL 
      LGIRVFTRS LSSYVPTAY LPVYKSVVA LANTSKIIE 
      VKLFPFTKN LKIEPHFVI SGLGFLFQM IRMCLSQYN 
      VNTKVNQLL CWVILLLTS WLRFSKTYT PYILFIEAF 
      SCIYLLSTA FTKPEPKKP NPKPTGMRK LVKNLEYTT 
      SDISDCSDF EDISDKSNS SLNTATVLN SSFKSAPDL 
      NRSLNNLSL GFKPPPRPK PVLSPPKLQ TVNSWVAGG 
      FWREGEGLL LPPAQRTNL SRCSSESSG FGSQLEAPF 
      VPKYYEFDR FSHVSEPNF RGFHPRFDA YQRKSSENW 
      SEKINSQAM MSFRDLSLR SNY
    CDS sequence
    • >TC011807 RA
      atgttagat ttacttccc tttttatca actatttta 
      cccataata gcctcaagc cttgttatc tgcgttttt 
      gtggtaaat ctttatttg aggataaga cgcaaattt 
      ccaattagt gttaattgc tggttttgc aactcatgg 
      accaaagta ccatacgac gaccgggac ggtttcgat 
      tgcccaact tgctcgcaa tataacggt ttcacgagc 
      gacggcggt tacaataag ataatccca gagcaacat 
      gacgagttt atgaataaa tcgacgagg gttaaaagc 
      cgcaaaagt ggcgcctcg aatgggctg tgcgctctg 
      tgtaataac aaccagcag ttgaaggtg ttccaactc 
      gcccacttt gttcccatg aacgaggag aattacgac 
      gtggaaatt gagcatttc caggaacag ctggaaaag 
      gcgtacaag ttatgcaag aaatgtgac aaggtcttg 
      aaaaagacc ataagcaag caaaacgcc tggatattc 
      ggaaaccgc gtcagggat ttatgccac aacggaatt 
      tccaaaatt gcaagccag atcagaaag gagacaaaa 
      cttacacta gtgagaaaa cttgacggc tgtttatca 
      attttatgc gttctcgcg gttattttt accactttg 
      ttgggaatt agagttttt acgcgaagt ttatccagt 
      tatgttcca acggcctat ttacctgtt tacaaaagt 
      gttgttgct ctagccaac acttcaaaa atcatcgaa 
      gttaaattg ttccctttc acgaagaat ctcaaaatt 
      gaacctcat tttgtcatt tctggccta ggttttttg 
      tttcaaatg attagaatg tgtttgagt caatacaat 
      gtgaacacg aaagtgaat cagttgctt tgttgggtg 
      attcttttg ttaacgtcg tggctgcgt tttagcaaa 
      acttataca ccgtatatt ttatttatt gaggctttt 
      tcctgcatt tacctgctc agcactgcc ttcacaaaa 
      cccgagcca aagaagcct aaccctaag ccaaccggg 
      atgcgcaaa ctggtcaaa aacctggaa tacacaacc 
      agcgacatc tccgactgt agcgacttt gaagacatt 
      tcagacaag tccaactcg agccttaac actgccaca 
      gtacttaac tcaagtttc aaaagcgcc cccgacttg 
      aaccgcagc ctcaacaac ctgagccta ggcttcaag 
      ccgcccccg cgcccaaaa cccgtcttg tcgcccccc 
      aaactccaa accgtcaat tcgtgggtg gccggcggg 
      ttttggcgc gagggcgaa ggcctgctc ttgcccccg 
      gcgcagcgg accaacctc agtcggtgc tccagcgag 
      agctcgggc ttcggctcc caactggag gcccccttt 
      gtcccgaaa tactacgaa ttcgaccga ttctcgcac 
      gtttcggag ccgaacttc cgggggttt cacccgcgg 
      ttcgacgcc tatcagcgg aaaagctcc gaaaactgg 
      tcggagaaa atcaactcg caagccatg atgagtttc 
      cgggacttg agtctgcgc tcgaattat tga
    mRNA sequence
    • >TC011807 RA
      cgccggtct cttgtcggt gtaaggtta tgtccaaag 
      ccgccaagt tggggcgag tttgggcat tcccttgtg 
      ttcacaatg ttagattta cttcccttt ttatcaact 
      attttaccc ataatagcc tcaagcctt gttatctgc 
      gtttttgtg gtaaatctt tatttgagg ataagacgc 
      aaatttcca attagtgtt aattgctgg ttttgcaac 
      tcatggacc aaagtacca tacgacgac cgggacggt 
      ttcgattgc ccaacttgc tcgcaatat aacggtttc 
      acgagcgac ggcggttac aataagata atcccagag 
      caacatgac gagtttatg aataaatcg acgagggtt 
      aaaagccgc aaaagtggc gcctcgaat gggctgtgc 
      gctctgtgt aataacaac cagcagttg aaggtgttc 
      caactcgcc cactttgtt cccatgaac gaggagaat 
      tacgacgtg gaaattgag catttccag gaacagctg 
      gaaaaggcg tacaagtta tgcaagaaa tgtgacaag 
      gtcttgaaa aagaccata agcaagcaa aacgcctgg 
      atattcgga aaccgcgtc agggattta tgccacaac 
      ggaatttcc aaaattgca agccagatc agaaaggag 
      acaaaactt acactagtg agaaaactt gacggctgt 
      ttatcaatt ttatgcgtt ctcgcggtt atttttacc 
      actttgttg ggaattaga gtttttacg cgaagttta 
      tccagttat gttccaacg gcctattta cctgtttac 
      aaaagtgtt gttgctcta gccaacact tcaaaaatc 
      atcgaagtt aaattgttc cctttcacg aagaatctc 
      aaaattgaa cctcatttt gtcatttct ggcctaggt 
      tttttgttt caaatgatt agaatgtgt ttgagtcaa 
      tacaatgtg aacacgaaa gtgaatcag ttgctttgt 
      tgggtgatt cttttgtta acgtcgtgg ctgcgtttt 
      agcaaaact tatacaccg tatatttta tttattgag 
      gctttttcc tgcatttac ctgctcagc actgccttc 
      acaaaaccc gagccaaag aagcctaac cctaagcca 
      accgggatg cgcaaactg gtcaaaaac ctggaatac 
      acaaccagc gacatctcc gactgtagc gactttgaa 
      gacatttca gacaagtcc aactcgagc cttaacact 
      gccacagta cttaactca agtttcaaa agcgccccc 
      gacttgaac cgcagcctc aacaacctg agcctaggc 
      ttcaagccg cccccgcgc ccaaaaccc gtcttgtcg 
      ccccccaaa ctccaaacc gtcaattcg tgggtggcc 
      ggcgggttt tggcgcgag ggcgaaggc ctgctcttg 
      cccccggcg cagcggacc aacctcagt cggtgctcc 
      agcgagagc tcgggcttc ggctcccaa ctggaggcc 
      ccctttgtc ccgaaatac tacgaattc gaccgattc 
      tcgcacgtt tcggagccg aacttccgg gggtttcac 
      ccgcggttc gacgcctat cagcggaaa agctccgaa 
      aactggtcg gagaaaatc aactcgcaa gccatgatg 
      agtttccgg gacttgagt ctgcgctcg aattattga 
      cttttattt tgtgatttg gataagtgt taagtttgg 
      aaaaatcaa aaacgaagg gttttttat cactgccat 
      gagctacct cgacttttt gattttcta ctagattta 
      agaataacg cggtttgaa tttgccgcc acaactgcc 
      aaattaagg cacgacgtt tgaattaat tgtatgaga 
      taagctgta tttgtttta aatacattt ttttgaaac 
      ttttggttt ttctggtat gcattccca cgttatggg 
      gttcagatg ggggggtct ggggaccgg gaccaagtc 
      ttgaggggg taccacctt aaatgttgg tcggtttct 
      taagttaag ggccatggt tgttaggtt ggtagcgtc 
      cattatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY