Status
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Gene information
General information
TC000635 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8739004..8763276
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000635
    iBeetle screen iB_06507

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03056

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000635 RA
      MRWSTLWWF AAVLAAALL QENGGEAAR TSRSNDMLE 
RLIKLDEMY SAVARPSVR SGPTQVDSM GPKVSIRVR 
TSATGCPFE DEEFSTVDF APFPAPSPV DNHTDFLSS 
KLMQITCYL LVTGCTGHV SAMGFVLLL LLLMCGSMS 
AFQDCGRSV RRSRQPRVG ALGRIIHGK QSVRGAWPW 
QVSLQLLHP QFGFLGHWC GGVLISPEW LLTAAHCVN 
NDLFNLPLA ALWTAVLGD WDRDVEEKS EQRIPVEEI 
ILHERFHNF QHDIALMKL SRPVKLARD SRVRAVCLP 
PSRLAYNQT DLCIATGWG RDAEDGMLA GKLLEARVP 
LHDNAVCRK KYGHAVSIR SGHMCAGHL DGSSGTCVG 
DSGGPLQCA MRDGRWMLA GITSFGSGC AKPGFPDVY 
TRLSYYLPW IQSKTRLKQ P
    CDS sequence
    • >TC000635 RA
      atgaggtgg tcgactctg tggtggttt gctgcggtc 
cttgccgcc gctctgctg caggaaaac gggggcgaa 
gccgcccgc acttctcgg agcaacgat atgttggag 
agattgatc aaattggac gaaatgtac tccgcagtg 
gccaggcct agtgtgcgc agcggaccg acccaagtt 
gatagtatg ggtcccaag gtcagtatc agagtgagg 
acgtcggcg actggttgc ccgttcgaa gatgaggaa 
ttctctacc gtagatttc gcccctttt ccagccccc 
agccccgta gacaatcac actgacttc ttatcatca 
aagctcatg cagataacc tgttactta ttagttacg 
gggtgcact ggacatgtt agtgctatg ggcttcgtc 
ttgctgctg ctcctcctg atgtgtggg tcaatgtca 
gcgttccaa gattgtgga aggagcgtc aggagatcc 
agacagccc cgcgtcggc gcccttgga cgcatcatc 
cacggcaag cagagtgtc agaggtgcg tggccgtgg 
caggtctcc ctgcagttg ttgcacccc caattcggg 
tttctgggt cactggtgc gggggtgtg ttgatatcg 
cccgaatgg ttgttaacg gcggctcat tgcgtcaac 
aacgacttg tttaacctt cccctggcc gccctctgg 
acggccgtt ttgggcgac tgggaccgc gacgtggag 
gaaaagtcc gagcagcgc attccagtc gaagaaata 
atcctgcac gagcgattt cacaatttt caacacgac 
attgctcta atgaaactc tcacggcca gtgaaatta 
gcaagagac agccgcgtt cgagctgtt tgcctgcct 
cccagccga ttagcatac aatcagacc gacctgtgt 
atcgccacc ggctggggc cgggacgcc gaggacggc 
atgctggcc gggaagctg ctggaggcg agagtgccg 
ctgcacgac aacgccgtc tgccggaaa aagtacggg 
catgccgtg tcgatccgg tcggggcat atgtgcgcc 
gggcacctg gacggctcg agcggcacc tgtgttggc 
gactccggc gggccgctc cagtgcgcc atgcgggac 
ggcaggtgg atgctggcg gggatcacc tccttcggc 
tccgggtgc gccaagccc ggattcccg gacgtctac 
acgcgactc tcctattat ctcccgtgg attcagtcc 
aaaacgcgg ctgaagcaa ccttga
    mRNA sequence
    • >TC000635 RA
      agtctcaac gcaacatat ccaaagttt caacaggtg 
atcttccga gtgctaccg gcacactta ccgttcgaa 
gttcgcagg aatatgagg tggtcgact ctgtggtgg 
tttgctgcg gtccttgcc gccgctctg ctgcaggaa 
aacgggggc gaagccgcc cgcacttct cggagcaac 
gatatgttg gagagattg atcaaattg gacgaaatg 
tactccgca gtggccagg cctagtgtg cgcagcgga 
ccgacccaa gttgatagt atgggtccc aaggtcagt 
atcagagtg aggacgtcg gcgactggt tgcccgttc 
gaagatgag gaattctct accgtagat ttcgcccct 
tttccagcc cccagcccc gtagacaat cacactgac 
ttcttatca tcaaagctc atgcagata acctgttac 
ttattagtt acggggtgc actggacat gttagtgct 
atgggcttc gtcttgctg ctgctcctc ctgatgtgt 
gggtcaatg tcagcgttc caagattgt ggaaggagc 
gtcaggaga tccagacag ccccgcgtc ggcgccctt 
ggacgcatc atccacggc aagcagagt gtcagaggt 
gcgtggccg tggcaggtc tccctgcag ttgttgcac 
ccccaattc gggtttctg ggtcactgg tgcgggggt 
gtgttgata tcgcccgaa tggttgtta acggcggct 
cattgcgtc aacaacgac ttgtttaac cttcccctg 
gccgccctc tggacggcc gttttgggc gactgggac 
cgcgacgtg gaggaaaag tccgagcag cgcattcca 
gtcgaagaa ataatcctg cacgagcga tttcacaat 
tttcaacac gacattgct ctaatgaaa ctctcacgg 
ccagtgaaa ttagcaaga gacagccgc gttcgagct 
gtttgcctg cctcccagc cgattagca tacaatcag 
accgacctg tgtatcgcc accggctgg ggccgggac 
gccgaggac ggcatgctg gccgggaag ctgctggag 
gcgagagtg ccgctgcac gacaacgcc gtctgccgg 
aaaaagtac gggcatgcc gtgtcgatc cggtcgggg 
catatgtgc gccgggcac ctggacggc tcgagcggc 
acctgtgtt ggcgactcc ggcgggccg ctccagtgc 
gccatgcgg gacggcagg tggatgctg gcggggatc 
acctccttc ggctccggg tgcgccaag cccggattc 
ccggacgtc tacacgcga ctctcctat tatctcccg 
tggattcag tccaaaacg cggctgaag caaccttga 
ctgacactg acccgatct cagatgtga tagtaattg 
ttcgtgaaa gtaacaaac ttgtaaata aaatgtata 
taaaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY