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Gene information
General information
TC012678 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13050119..13052610
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012678
    iBeetle screen iB_06506

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012678 RA
      MDFTSYRRT VRKAIAEEP LSELYPHDV SMYRESPKS 
      EISILEFEQ IALDRLKLL RIIEQAAPK GNRLYSDQW 
      KTCIKDDLA KAGLKKYVR LMGGTSGTS DLDYQARKA 
      DYLSHFILR LAYCRSEQL RRWFLSREL EWFWIRFTA 
      QSREGVKNF LRLYKFSYT PISEEEKVS LRHELEAST 
      AGLILFEET EFFKVPFTE VCSLVRGRK VFLKAGFAY 
      IPSSELVVC IQSKFRAEL SEELTWYSH RIHLFDDER 
      IGPLLSNFH NLHTGQNYR VRENHESVD PADLDAYAK 
      KHFPLCMKH MHEVLRSEH HHKHQGRLT YGLFLKAIG 
      LLYEDALKF WRDEFTKKM DLNQFEKGY VYNIKHMYG 
      LVGKRASYS PYSCVKIIT SSVGPGEHH GCPFKHWDV 
      NLVKQKVIE CGVSVDGAN EIKQLVSDG HYQLACGKY 
      FEYTHGKAL GNGINHPNQ YFEESMNIE KGASGNKK
    CDS sequence
    • >TC012678 RA
      atggatttc acgtcgtat cgccgcacg gtgcgaaaa 
      gccatcgcc gaggagccc ctctccgag ctgtacccc 
      cacgacgtg agcatgtac cgcgaaagc cccaaaagc 
      gagatttcc atcctggaa ttcgagcaa atcgccctg 
      gaccggctc aaattgctg cgaattatc gagcaagcg 
      gcccccaaa ggcaaccgg ttgtacagc gaccaatgg 
      aagacgtgc attaaggat gacttggcc aaggcaggg 
      ttgaaaaaa tacgtgcgc ttgatgggg ggcaccagt 
      ggcacgagc gacttggat taccaagcc cgcaaggcc 
      gactacctc tcacacttt atcctccgg ctggcgtat 
      tgccgctcg gagcaattg cgacggtgg tttttgagc 
      agggaactc gagtggttt tggatccgt ttcacggct 
      cagtcgcgc gagggggtc aagaatttt ttacgactt 
      tataaattc tcatatact cctatttcg gaggaggag 
      aaggtttcg ctaaggcat gaactggag gcgtcaact 
      gcggggttg attctgttc gaggagact gaatttttt 
      aaagtgcct tttactgaa gtttgctcg ttggtgagg 
      ggccggaaa gtgtttctt aaggctggg tttgcctat 
      attccgtcc agcgagctg gtggtctgc atccagtcg 
      aagttcagg gctgaatta agcgaagag ctaacttgg 
      tacagccac cgcatccac cttttcgac gacgagcgt 
      attgggccc cttttgtcc aatttccac aatttgcac 
      acgggccaa aattaccga gtccgtgaa aaccacgaa 
      agtgtcgat ccagcagat ctggacgca tatgctaaa 
      aaacacttt cctctctgc atgaaacac atgcatgag 
      gttttgcgg tctgaacac caccataaa caccaaggc 
      cgtctaact tacggtctt ttcttgaaa gctatcggg 
      cttttgtac gaagatgcg ctcaaattt tggagggat 
      gagttcacg aaaaaaatg gatttgaat cagtttgag 
      aagggttac gtttacaat atcaaacac atgtatggg 
      ttggtgggg aaaagggcc agttattcg ccctacagt 
      tgtgtgaaa attattacg agtagtgtg gggcctggg 
      gagcatcat ggctgtccg tttaaacac tgggatgtt 
      aatcttgtt aagcagaaa gttatcgaa tgtggtgtg 
      agcgttgat ggtgctaat gaaatcaag cagcttgtt 
      tctgatggt cattatcaa ctagcttgt ggaaaatat 
      ttcgaatac actcacgga aaagcgctg ggtaatgga 
      attaatcac cctaatcag tattttgaa gagagcatg 
      aatattgaa aaaggagct tcaggcaat aagaaataa
    mRNA sequence
    • >TC012678 RA
      ggggtggaa cgccacacc tggaaaata tctgagcgt 
      tggttttgc ccccaaaaa ccgggcagt gattttagc 
      tagtcccca gatattggt gccagtttt tggcgggat 
      accatttaa atgtgaggt taagtgagt taagtttta 
      actaaacac tggaaattg tgaattttc gccatggat 
      ttcacgtcg tatcgccgc acggtgcga aaagccatc 
      gccgaggag cccctctcc gagctgtac ccccacgac 
      gtgagcatg taccgcgaa agccccaaa agcgagatt 
      tccatcctg gaattcgag caaatcgcc ctggaccgg 
      ctcaaattg ctgcgaatt atcgagcaa gcggccccc 
      aaaggcaac cggttgtac agcgaccaa tggaagacg 
      tgcattaag gatgacttg gccaaggca gggttgaaa 
      aaatacgtg cgcttgatg gggggcacc agtggcacg 
      agcgacttg gattaccaa gcccgcaag gccgactac 
      ctctcacac tttatcctc cggctggcg tattgccgc 
      tcggagcaa ttgcgacgg tggtttttg agcagggaa 
      ctcgagtgg ttttggatc cgtttcacg gctcagtcg 
      cgcgagggg gtcaagaat tttttacga ctttataaa 
      ttctcatat actcctatt tcggaggag gagaaggtt 
      tcgctaagg catgaactg gaggcgtca actgcgggg 
      ttgattctg ttcgaggag actgaattt tttaaagtg 
      ccttttact gaagtttgc tcgttggtg aggggccgg 
      aaagtgttt cttaaggct gggtttgcc tatattccg 
      tccagcgag ctggtggtc tgcatccag tcgaagttc 
      agggctgaa ttaagcgaa gagctaact tggtacagc 
      caccgcatc caccttttc gacgacgag cgtattggg 
      ccccttttg tccaatttc cacaatttg cacacgggc 
      caaaattac cgagtccgt gaaaaccac gaaagtgtc 
      gatccagca gatctggac gcatatgct aaaaaacac 
      tttcctctc tgcatgaaa cacatgcat gaggttttg 
      cggtctgaa caccaccat aaacaccaa ggccgtcta 
      acttacggt cttttcttg aaagctatc gggcttttg 
      tacgaagat gcgctcaaa ttttggagg gatgagttc 
      acgaaaaaa atggatttg aatcagttt gagaagggt 
      tacgtttac aatatcaaa cacatgtat gggttggtg 
      gggaaaagg gccagttat tcgccctac agttgtgtg 
      aaaattatt acgagtagt gtggggcct ggggagcat 
      catggctgt ccgtttaaa cactgggat gttaatctt 
      gttaagcag aaagttatc gaatgtggt gtgagcgtt 
      gatggtgct aatgaaatc aagcagctt gtttctgat 
      ggtcattat caactagct tgtggaaaa tatttcgaa 
      tacactcac ggaaaagcg ctgggtaat ggaattaat 
      caccctaat cagtatttt gaagagagc atgaatatt 
      gaaaaagga gcttcaggc aataagaaa taatatctg 
      ttactacaa ttaaacgtt attttttaa taatttatt 
      ttattttac ccttcagtc taaattgca tccgttttt 
      ttaatttgg ttttaatag tcccaaaga ctttaattg 
      ttgatacat gctttcacg ttgtatttt atagttaaa 
      taaatattt tggagaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY