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Gene information
General information
TC011831 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13037363..13039200
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC011831
      iBeetle screen iB_06505

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011831 RA
        MNESNSREL QRAQTHLRT LFRAFVQVS 
ESSLNRKNQ QLLDDLDKL EFGAPLPEL 
AQFAGELPE LPSVDPTEL RSEMVKALQ 
LLQVLKNKA DCPISRIVD EV
      CDS sequence
      • >TC011831 RA
        atgaacgag tcaaacagc cgggagctg caaagggcg 
cagacccac ttgcggacc cttttccgg gcgttcgtc 
caggtcagt gagagcagt ctgaaccgc aaaaaccag 
cagcttctg gacgacctc gacaagctc gagtttggg 
gcaccgctc ccggagttg gcccagttt gccggggag 
ttacccgag ttgccgtca gtggacccc acggagcta 
aggagcgag atggtcaag gcgcttcag ctgctccag 
gtgctcaag aacaaggcg gactgcccg atatcgcgt 
atcgttgat gaggtttga
      mRNA sequence
      • >TC011831 RA
        ccttatttc tcaatatgt gactttggg ccccaaaca 
gtccctgat aatgacccg gaggtcgaa tctttaatt 
tgagacgaa cgcggttgg cgacagtgt ccgccgcca 
ttgtcacga tttgaaaag ttaacctca cttgtgccg 
tcaaccgtc cagattatt tggctttaa atatgaacg 
agtcaaaca gccgggagc tgcaaaggg cgcagaccc 
acttgcgga cccttttcc gggcgttcg tccaggtca 
gtgagagca gtctgaacc gcaaaaacc agcagcttc 
tggacgacc tcgacaagc tcgagtttg gggcaccgc 
tcccggagt tggcccagt ttgccgggg agttacccg 
agttgccgt cagtggacc ccacggagc taaggagcg 
agatggtca aggcgcttc agctgctcc aggtgctca 
agaacaagg cggactgcc cgatatcgc gtatcgttg 
atgaggttt gagtaagtg agtggaggt cacattgaa 
aatttatta tccaaaagt tatgaagaa acatggatt 
aataatata caataaata ttcacatat aaaataaca 
tcaataagt aaaatatgg aacgttatt acctaccaa 
taattctgt aagatttgt caccgacaa atggaagaa 
taaatattt tctcataag aataattac aaagttaaa 
ttcaatagg aatgttgca aacatagtg aaacactga 
ctcaccaat tacgtgata accggggcg cggcgccct 
tttgcttaa ataagaaaa taacaaaca ttcattata 
tagggctct gcgttactg tctaacagt aagtaaaca 
ttacatatt tctatgttt gacactttc atgtcgcga 
aaatgccgt ccctacgtc taatgactg gatcctttc 
ttttaatat aatataatg cacagcccc ccaaaactg 
acgcgtcag gaggctgcg ataatcact actgagtta 
agctatgta taacctatg tcatgaaaa tatatactt 
taacattta ttcgtacgt acaataatt tctctatgg 
ataacgata tacagcata acaatttgt gtgtctact 
tatggcaag attgttcct acatgcatg gaaaaataa 
ataatatgg cactaatgt tacaaaatg ttccatata 
cagggcgag acaacaaca ggccaccgg agcacgttt 
gaaattatt aaaattgag gccatttta ataataccc 
aaatcgtcg gtgcaaaaa cacccactc tgtatattt 
agcaatttt cgaaacatt tcgtacgta taatcctaa 
aatacatct cgatatgca gtacaattt gtaaaatct 
taattctcg taaacaagt agaaattgt gccctttaa 
tagcaagta atattaagt aggttgtcg tataaaata 
caaaagtta taaatacac gctcaattg tttgcaggc 
tgcccaaca gtctttaag aaaataaac gcttccgtc 
aacaatatt catttggtg gtgggatta agttggcaa 
cggtgctcc aacacagtt aatatcacc aatcattca 
aggttgcca acttattcc ccgtccatt cacggtcag 
accggctgt ggaaattga cagttttgt aactggtga 
cttgatttc gaaaaatta actacggag gactataag 
ttaggagac gaaacgaga tacaaaaac gtacccaga 
aaatgagac tgctttaaa ttaatttaa attcaacca 
actgcctgt atgtttttg ttt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY