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Gene information
General information
TC011843 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12668698..12670594
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011843
    iBeetle screen iB_06503

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011843 RA
      MDLKNETPS KSKNLMSKV LSNPMNCDS PFRLLPNVS 
      TPPSRFAKI LNPFEPHLI DRMHLPTFS PSVFAQVST 
      PKTDKFKWT IDEISSLKP ADIDEDTLS QHVSDHDPT 
      IESLVQEKI DKFFKEKAI VPSPMTQPC QQLPALMSP 
      PRSDTCAQT ALSLPPVLP KHVEEALAP YFTYQEKDE 
      FGDSSLYRK LFEFEGHSS PATSSPAPS TGLSPIQFS 
      PEFSPHSPK AKLESTLEM PELRDCTLS PIGRSPLSR 
      SACRLSFSA HMSVDASMV VPDVNRSLQ VTRLEQEVS 
      FQPLEDGGP MDSLVNWDM EYKQVSILS RSPVSSPER 
      LDSSNGDTP HSRIFTSQR KRLSDSFKD EEFERDVEM 
      VEDVIMKRV KNRLGEDTT DGGYHTGGV TLCESSTHM 
      FASTPTKSK H
    CDS sequence
    • >TC011843 RA
      atggattta aaaaacgaa actccttcg aaatcgaag 
      aacctgatg agcaaagta ttaagcaac ccgatgaac 
      tgtgatagc cccttcaga ctcctgccc aacgtctcg 
      acgccgcct tcaaggttc gccaagatc ctaaacccc 
      ttcgagccc cacttgatc gaccgtatg cacctcccg 
      accttcagc cccagtgtt ttcgcccaa gtctcaacc 
      ccaaaaacg gacaaattc aagtggaca atcgacgag 
      atctcgagc ttgaagccg gccgacatc gacgaagac 
      acactctcg cagcacgtc tcggaccac gaccccaca 
      atcgagtcg ctcgtgcag gaaaaaatc gacaagttt 
      ttcaaggag aaagcgatc gtccccagc cccatgacc 
      caaccctgc cagcaactg cccgctttg atgtcgccc 
      ccgcgctcc gacacgtgc gcccagacc gcgctctcc 
      ctgccccca gtgctcccc aagcacgtg gaggaggcc 
      ctcgccccc tattttaca tatcaggag aaggacgag 
      tttggggac tcgtccctt taccgcaaa ctgttcgag 
      ttcgagggg cacagcagc cccgccacg tccagccct 
      gcgccctcc acggggctc tcccccatc cagttctcg 
      ccggagttc agcccgcat tcgcccaag gcgaagctc 
      gagagcacg ctggagatg cccgagttg cgggactgc 
      accctgtcc cccatcggg cgctcgccc ctgtcccgg 
      tccgcgtgc aggctcagc ttttcggcg cacatgtcg 
      gtggatgcg agcatggtg gtcccggac gtgaatagg 
      agtctccag gtgaccagg ctcgagcag gaggtttcg 
      ttccagccc ctggaggat gggggcccc atggactcg 
      ctggtcaac tgggacatg gagtataag caagtttcg 
      attttgtcg cggtctcca gtgagcagc cccgaacgc 
      ttggattct tcaaatggt gatacgccc cattcccga 
      attttcact agtcagaga aaaagactg tcggatagt 
      tttaaagac gaagagttt gagcgtgac gttgaaatg 
      gtggaggat gtgattatg aagcgggtg aagaataga 
      ctgggtgaa gacaccact gatggtggg tatcacacg 
      gggggcgtc accttgtgt gagagttcc acgcatatg 
      tttgcgtca acgccgacc aaaagcaag cactga
    mRNA sequence
    • >TC011843 RA
      ggcttagga agctaatat ttattatta caagagata 
      aaattagga aaagggacg tctagggag tttggtgtt 
      atttgcacc tggccacca cttggcgga aagtcaaac 
      ggttaaaag tcaaattcg aacgtcata acggtcaag 
      tgagagttt ttgtgttca ttttaattc taagtcgta 
      aaaaccgta cattttgtg ataccgtcg taatgttag 
      tgttagtta attaataaa atggattta aaaaacgaa 
      actccttcg aaatcgaag aacctgatg agcaaagta 
      ttaagcaac ccgatgaac tgtgatagc cccttcaga 
      ctcctgccc aacgtctcg acgccgcct tcaaggttc 
      gccaagatc ctaaacccc ttcgagccc cacttgatc 
      gaccgtatg cacctcccg accttcagc cccagtgtt 
      ttcgcccaa gtctcaacc ccaaaaacg gacaaattc 
      aagtggaca atcgacgag atctcgagc ttgaagccg 
      gccgacatc gacgaagac acactctcg cagcacgtc 
      tcggaccac gaccccaca atcgagtcg ctcgtgcag 
      gaaaaaatc gacaagttt ttcaaggag aaagcgatc 
      gtccccagc cccatgacc caaccctgc cagcaactg 
      cccgctttg atgtcgccc ccgcgctcc gacacgtgc 
      gcccagacc gcgctctcc ctgccccca gtgctcccc 
      aagcacgtg gaggaggcc ctcgccccc tattttaca 
      tatcaggag aaggacgag tttggggac tcgtccctt 
      taccgcaaa ctgttcgag ttcgagggg cacagcagc 
      cccgccacg tccagccct gcgccctcc acggggctc 
      tcccccatc cagttctcg ccggagttc agcccgcat 
      tcgcccaag gcgaagctc gagagcacg ctggagatg 
      cccgagttg cgggactgc accctgtcc cccatcggg 
      cgctcgccc ctgtcccgg tccgcgtgc aggctcagc 
      ttttcggcg cacatgtcg gtggatgcg agcatggtg 
      gtcccggac gtgaatagg agtctccag gtgaccagg 
      ctcgagcag gaggtttcg ttccagccc ctggaggat 
      gggggcccc atggactcg ctggtcaac tgggacatg 
      gagtataag caagtttcg attttgtcg cggtctcca 
      gtgagcagc cccgaacgc ttggattct tcaaatggt 
      gatacgccc cattcccga attttcact agtcagaga 
      aaaagactg tcggatagt tttaaagac gaagagttt 
      gagcgtgac gttgaaatg gtggaggat gtgattatg 
      aagcgggtg aagaataga ctgggtgaa gacaccact 
      gatggtggg tatcacacg gggggcgtc accttgtgt 
      gagagttcc acgcatatg tttgcgtca acgccgacc 
      aaaagcaag cactgaacg agattaagg cctgttttt 
      ttattaatt aattaacta gtggcaagg taagtttta 
      agatactat cgtgactaa aaatgcttg tgttcgttt 
      ttacaaaac tagaagtct tttttggtt tgtagtgga 
      caaaacaat tatttttgt ttatgtgca atcgcccca 
      ataaatgtt atacaagac t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY