| General information | |||
| TC011886 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 12228718..12237182 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011886 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06498 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC011886 RA
MATLNPVQW EKPLSLDRQ SPSLHVLEV PLPSLQNPS FLHSQKLRE NLIESRKAF LRNLNEDEK LEPNNEPRK TPTKKISFK IKKTASIYR HNQKMSTIS ASKVAELTM KFNMINEKN SLLEQPKFR KLVKRVNSQ RSLDSDKVT KHSVKNVVT RKPSVKTKP YEDKEIVIV RRKPTIKRK PSRANLEKP VLVTEQNRP TTLPLDEPQ PPSTGTVRA AIEIFERRN SSPVPKPKV PEKPTKLKP DKTDEDLVQ IVNIPAVLP QRRCDSMYE TLNVTKTPL LKPTKSEET IVRPKPNTS FLWSINQDK SPSAKDDWK DLYDFVDPS PQVPPRAAP AKPPTPPKK TFMKPPPEE KIYEELNVS RDEDTISHS YEYCEGDDG YEICAKENI YETLPPPLL PRRQQQEPL PPRPPSRSS YCTIQNPEN VSNCYESIY NPESKSNES TYESIYGCQ IREGGSNRD SLVSSDQQS NSLYGRASL VGWGEDGTN PYSGKATSD LSTSDRSDD WVDMTDNED NDNNNEIII IRERQKAKK TTGWSQQFR EQWSKSPRK LPTDQRTDS DPDHLYERL CTNENTQDD DYDSFDSDS DSEGSFTKV NSHDSGLDI GNSQLPEPP NNQTYVLTR LAATAGKHM RKLRRNWSL TKNDISKSL SRMTKRKSR TDLAKHRKS SPEILQPEE PPPPCDNPP EAEAKTLPR KSFLTKFRR SMSLSAESA SELTSNLDK PKSTFYLTE IDIDKNESE RDSGASLSP VQRNSKVIT RPQSPPPPA PVREKKRST SWYAEVGLF KNSESNAQR RSNTFWYAE VGLYQTGMS TPSTSSAEN SGNTTNVSI KPDDFINQD NSEEYYNLK NGSDYYNDS INSFSSTET KKDQSLTTL ATDAHLRLE DEPLYQFYD AAVLESVCH DGVSDFDSD GYEEVGDNN SESTLSSRP SAMELVTPN KNLLTVSRT LWCEIPEVI QSSVLSTLS THQKKLQEA KFEMITSEA SYLNSLNVL CNHFVKSFE SLDILSKDE LEMLFGKVA DVKNCSEKL ISDLEKCWQ ENILLHGVC DIVQKHAEE NFHVYVPYC ENQILLDDT LRRIKERPN FLEQLKQLE SSSTCQSLT LYSFLMLPM QRITRWPLL VDAVLKRLS PQDSEYLTC QYALATLNK IVSQCNEGA RRKERENEM KKIVTQLEF AKGVPPIEI VSDNRWLIR SGTVTHMQQ RNDEIKLTF GKRFTKVTL HLFLFNDYL FVTKPKSEN LYTVVHYCA RNLVELSST DMLVSLPVK DTQGRHLLF LTILENQND KTVEFLLSC NSESDKERW IEALTPPKS EDPNETLYE CWDCPQVTA IHNYLASQP DELALSRGD VINVLRKMA DGWYHGERI RDGQTGWFP ANYTVEIAN PHVRSRNLK QRYRLLAFS ENYLKTK
- >TC011886 RA
atggcaacc ttaaatcca gttcagtgg gagaagcct ttgtcgttg gaccgacaa agtccatct ctccacgtc ctggaagtc ccgcttcca agcctccaa aacccgtcg ttcctccac tcacaaaag ctgcgagag aacctcatc gaatcccgc aaggcgttc cttcgcaac ctcaatgaa gacgaaaag ctagaaccc aacaatgaa ccccggaaa acccccacg aaaaaaatc agcttcaaa atcaaaaaa accgcctcg atctaccgc cacaaccag aaaatgtcc acgatcagt gcaagcaaa gtcgccgaa ctcacaatg aagttcaac atgatcaac gagaagaac tccctcctg gaacagccc aagttccgc aagctcgtc aagcgcgtc aacagccaa agaagctta gacagtgat aaagtgacc aagcacagt gttaagaac gtagtgacg cgcaaaccc agcgttaaa acgaaaccc tacgaggac aaagagatt gtcatagtg aggcgcaaa ccgacgatc aaacgcaaa cccagccgt gccaattta gaaaaaccg gtgctagtg accgaacaa aaccgcccc acaaccctc cccctcgac gaaccccaa ccgccgtcc acgggaacc gtccgtgcg gcgatcgaa attttcgaa cgcagaaac tcaagccca gtgccaaaa cccaaagtg cctgaaaaa ccaacgaaa ctaaaaccc gacaagact gacgaagat ttagtacaa atcgttaac attccggcg gtgctgccg caacgaaga tgtgattcc atgtacgaa acgctcaat gttacgaaa acaccactc ctaaaaccg accaagtcg gaggaaacc atcgttagg ccaaaaccc aacacttcc ttcctctgg agcatcaac caggacaag tcgccctcg gccaaagac gactggaag gacctctac gacttcgtc gacccctcc ccacaagtc cccccgagg gccgccccg gcgaaaccc ccaactccc ccgaaaaaa accttcatg aagcctcct cccgaggag aagatttac gaggagttg aatgtgagt cgggacgag gacacgatc agccacagc tacgagtac tgcgaaggg gatgatggg tacgagatc tgtgccaag gagaatatc tacgaaacg ctcccgccg ccgcttcta cccaggagg cagcagcag gaaccgctt ccgccaaga ccaccctcc cggagcagc tattgcacg attcaaaac ccggaaaac gtgtcaaat tgctacgaa tcgatctac aatcccgaa tccaagtcg aatgagagt acttatgag agtatctac gggtgccaa atccgcgag ggtggctcg aatcgcgat tctcttgtg tcctcggac cagcagagt aatagtttg tacgggagg gctagtcta gtggggtgg ggtgaggat gggacgaat ccctacagc ggcaaggcc acaagtgac ttaagcacg agtgaccgg agcgatgat tgggtcgat atgaccgat aatgaggat aatgacaac aacaatgaa atcatcata atccgcgag cgtcaaaag gcgaagaag actaccggt tggtcccag caatttcgg gagcaatgg tctaaatcg ccgcgaaaa ctcccaact gatcagcgt actgacagt gacccggac cacctctac gagagactt tgcaccaat gagaacacc caagacgac gattacgac tccttcgac agtgatagt gattcggaa ggttccttc actaaagtc aattcacat gacagcggc ctggacatc ggcaacagc caactcccc gaaccccca aacaaccaa acctacgtc ctgacccgt ctcgcggcc accgccggc aaacacatg cgcaagctg cgccgcaac tggtcactg accaaaaac gacatcagc aaatcactg agtcggatg accaaacgc aagtcgcgc acagacctc gccaaacac aggaaatct agtcccgaa attctccaa ccggaggag ccgccccct ccttgtgat aaccccccg gaggcggaa gccaagacc ctgcccagg aagagcttc ctgacgaag ttccggagg agcatgtct ctgagtgcc gagtcggcg agcgagctc acctcgaat ttggataag ccgaagagt acgttctac ttgacggaa attgacatc gataagaac gagtctgag agggacagc ggggcgtcg ttatcgccg gtgcagagg aactccaag gtcataacg cggccgcag agcccgccc ccgcccgca ccggtcagg gagaaaaag cggtcgact tcgtggtac gcagaagtg gggctgttt aaaaattcg gaaagcaac gcgcagagg aggagcaat acgttctgg tacgccgaa gtcgggctc taccagact gggatgagc accccgagc acgagttcg gcggaaaat agcggaaat acaaccaat gtttcgatt aaaccggac gattttatc aaccaggat aacagcgag gagtactac aatctcaag aacggctcg gattattac aatgacagt attaacagc tttagcagc acggagacc aagaaagat cagtctttg acgactctg gctactgac gctcacttg aggctggaa gatgagcct ttgtatcag ttttacgac gctgcggtt ttagagtcg gtttgtcac gatggtgtc tccgatttt gattccgac ggctatgaa gaagtcggt gataacaac tccgaatcc actctttcc tcaagacca tcagctatg gaactagtc acacccaac aaaaattta ctgacggtt tcaagaacc ctttggtgt gaaatacca gaagtgata cagtcgtcg gtgttaagc actttgagt acgcatcag aagaagttg caagaagct aagttcgaa atgatcacg tctgaagcc tcctacttg aactccctc aacgttctg tgcaaccac tttgtcaaa agtttcgaa agtttggat attttgtca aaggatgag ctcgaaatg ttgttcgga aaagtcgca gatgtgaaa aactgttcg gagaagctt atatccgac ttggagaag tgttggcag gaaaatatt ctgcttcat ggagtgtgt gatattgtt cagaagcac gctgaggag aatttccac gtgtatgtc ccctactgt gagaatcaa attttgtta gacgacacg ttaaggcgt atcaaggaa cgtccaaat tttctcgaa caattaaaa cagcttgaa tcaagctca acgtgtcag tcactcaca ctttactca tttttaatg ctcccaatg cagaggata acgaggtgg ccactgctt gtggacgcc gtcttgaag aggctctcg ccacaagac tccgaatat ttaacgtgt caatacgcg cttgcaacg ctcaataaa atcgtcagt cagtgcaac gaaggggca agacgtaaa gaacgcgaa aatgaaatg aaaaaaatc gtaacacag ttagagttc gccaagggg gttcccccg attgaaata gtgtcggac aatcggtgg ctcatccgc tccggtact gtaacccac atgcagcaa aggaacgat gagattaag ctgactttt ggaaaacgg ttcacgaaa gtgacgcta cacttgttt ttattcaac gattatttg tttgttaca aaaccgaaa agcgaaaat ttgtacacc gtggtccat tactgcgcc cgcaatttg gtggagttg agcagcacc gacatgttg gtgtccctc ccggtcaaa gacacgcaa ggtcgccac ttgctgttc ctcacaatt ttggaaaat cagaatgac aagactgtt gaatttttg ttgtcttgt aacagtgaa agcgacaag gagcgatgg atcgaggcg ctaacgccc ccaaaatct gaagaccca aacgaaact ctttacgaa tgttgggac tgtccgcaa gtaactgcc attcataat tatctggct agtcaaccc gacgaattg gctttatct cgaggggat gtgattaac gttctccgc aaaatggcc gatggttgg tatcacgga gagagaata agagatggc caaactggc tggttcccc gcgaattat actgtagaa atagcgaac cctcatgtc agatcacgt aatttgaaa cagcgatat cggttgttg gcgttttcg gaaaactat ttaaaaact aaatga
- >TC011886 RA
ctagacatt aagcacgtg tgtaaactg ggtttctgt gataatcca ataccagct acaatagct tgtcatcga tctacatgc tgtcatttt gttccagaa ttttgccaa ccatgagac cgcacatgt cgcatttga ataaaaaag tgtagtggc ctcgggacg atggcaacc ttaaatcca gttcagtgg gagaagcct ttgtcgttg gaccgacaa agtccatct ctccacgtc ctggaagtc ccgcttcca agcctccaa aacccgtcg ttcctccac tcacaaaag ctgcgagag aacctcatc gaatcccgc aaggcgttc cttcgcaac ctcaatgaa gacgaaaag ctagaaccc aacaatgaa ccccggaaa acccccacg aaaaaaatc agcttcaaa atcaaaaaa accgcctcg atctaccgc cacaaccag aaaatgtcc acgatcagt gcaagcaaa gtcgccgaa ctcacaatg aagttcaac atgatcaac gagaagaac tccctcctg gaacagccc aagttccgc aagctcgtc aagcgcgtc aacagccaa agaagctta gacagtgat aaagtgacc aagcacagt gttaagaac gtagtgacg cgcaaaccc agcgttaaa acgaaaccc tacgaggac aaagagatt gtcatagtg aggcgcaaa ccgacgatc aaacgcaaa cccagccgt gccaattta gaaaaaccg gtgctagtg accgaacaa aaccgcccc acaaccctc cccctcgac gaaccccaa ccgccgtcc acgggaacc gtccgtgcg gcgatcgaa attttcgaa cgcagaaac tcaagccca gtgccaaaa cccaaagtg cctgaaaaa ccaacgaaa ctaaaaccc gacaagact gacgaagat ttagtacaa atcgttaac attccggcg gtgctgccg caacgaaga tgtgattcc atgtacgaa acgctcaat gttacgaaa acaccactc ctaaaaccg accaagtcg gaggaaacc atcgttagg ccaaaaccc aacacttcc ttcctctgg agcatcaac caggacaag tcgccctcg gccaaagac gactggaag gacctctac gacttcgtc gacccctcc ccacaagtc cccccgagg gccgccccg gcgaaaccc ccaactccc ccgaaaaaa accttcatg aagcctcct cccgaggag aagatttac gaggagttg aatgtgagt cgggacgag gacacgatc agccacagc tacgagtac tgcgaaggg gatgatggg tacgagatc tgtgccaag gagaatatc tacgaaacg ctcccgccg ccgcttcta cccaggagg cagcagcag gaaccgctt ccgccaaga ccaccctcc cggagcagc tattgcacg attcaaaac ccggaaaac gtgtcaaat tgctacgaa tcgatctac aatcccgaa tccaagtcg aatgagagt acttatgag agtatctac gggtgccaa atccgcgag ggtggctcg aatcgcgat tctcttgtg tcctcggac cagcagagt aatagtttg tacgggagg gctagtcta gtggggtgg ggtgaggat gggacgaat ccctacagc ggcaaggcc acaagtgac ttaagcacg agtgaccgg agcgatgat tgggtcgat atgaccgat aatgaggat aatgacaac aacaatgaa atcatcata atccgcgag cgtcaaaag gcgaagaag actaccggt tggtcccag caatttcgg gagcaatgg tctaaatcg ccgcgaaaa ctcccaact gatcagcgt actgacagt gacccggac cacctctac gagagactt tgcaccaat gagaacacc caagacgac gattacgac tccttcgac agtgatagt gattcggaa ggttccttc actaaagtc aattcacat gacagcggc ctggacatc ggcaacagc caactcccc gaaccccca aacaaccaa acctacgtc ctgacccgt ctcgcggcc accgccggc aaacacatg cgcaagctg cgccgcaac tggtcactg accaaaaac gacatcagc aaatcactg agtcggatg accaaacgc aagtcgcgc acagacctc gccaaacac aggaaatct agtcccgaa attctccaa ccggaggag ccgccccct ccttgtgat aaccccccg gaggcggaa gccaagacc ctgcccagg aagagcttc ctgacgaag ttccggagg agcatgtct ctgagtgcc gagtcggcg agcgagctc acctcgaat ttggataag ccgaagagt acgttctac ttgacggaa attgacatc gataagaac gagtctgag agggacagc ggggcgtcg ttatcgccg gtgcagagg aactccaag gtcataacg cggccgcag agcccgccc ccgcccgca ccggtcagg gagaaaaag cggtcgact tcgtggtac gcagaagtg gggctgttt aaaaattcg gaaagcaac gcgcagagg aggagcaat acgttctgg tacgccgaa gtcgggctc taccagact gggatgagc accccgagc acgagttcg gcggaaaat agcggaaat acaaccaat gtttcgatt aaaccggac gattttatc aaccaggat aacagcgag gagtactac aatctcaag aacggctcg gattattac aatgacagt attaacagc tttagcagc acggagacc aagaaagat cagtctttg acgactctg gctactgac gctcacttg aggctggaa gatgagcct ttgtatcag ttttacgac gctgcggtt ttagagtcg gtttgtcac gatggtgtc tccgatttt gattccgac ggctatgaa gaagtcggt gataacaac tccgaatcc actctttcc tcaagacca tcagctatg gaactagtc acacccaac aaaaattta ctgacggtt tcaagaacc ctttggtgt gaaatacca gaagtgata cagtcgtcg gtgttaagc actttgagt acgcatcag aagaagttg caagaagct aagttcgaa atgatcacg tctgaagcc tcctacttg aactccctc aacgttctg tgcaaccac tttgtcaaa agtttcgaa agtttggat attttgtca aaggatgag ctcgaaatg ttgttcgga aaagtcgca gatgtgaaa aactgttcg gagaagctt atatccgac ttggagaag tgttggcag gaaaatatt ctgcttcat ggagtgtgt gatattgtt cagaagcac gctgaggag aatttccac gtgtatgtc ccctactgt gagaatcaa attttgtta gacgacacg ttaaggcgt atcaaggaa cgtccaaat tttctcgaa caattaaaa cagcttgaa tcaagctca acgtgtcag tcactcaca ctttactca tttttaatg ctcccaatg cagaggata acgaggtgg ccactgctt gtggacgcc gtcttgaag aggctctcg ccacaagac tccgaatat ttaacgtgt caatacgcg cttgcaacg ctcaataaa atcgtcagt cagtgcaac gaaggggca agacgtaaa gaacgcgaa aatgaaatg aaaaaaatc gtaacacag ttagagttc gccaagggg gttcccccg attgaaata gtgtcggac aatcggtgg ctcatccgc tccggtact gtaacccac atgcagcaa aggaacgat gagattaag ctgactttt ggaaaacgg ttcacgaaa gtgacgcta cacttgttt ttattcaac gattatttg tttgttaca aaaccgaaa agcgaaaat ttgtacacc gtggtccat tactgcgcc cgcaatttg gtggagttg agcagcacc gacatgttg gtgtccctc ccggtcaaa gacacgcaa ggtcgccac ttgctgttc ctcacaatt ttggaaaat cagaatgac aagactgtt gaatttttg ttgtcttgt aacagtgaa agcgacaag gagcgatgg atcgaggcg ctaacgccc ccaaaatct gaagaccca aacgaaact ctttacgaa tgttgggac tgtccgcaa gtaactgcc attcataat tatctggct agtcaaccc gacgaattg gctttatct cgaggggat gtgattaac gttctccgc aaaatggcc gatggttgg tatcacgga gagagaata agagatggc caaactggc tggttcccc gcgaattat actgtagaa atagcgaac cctcatgtc agatcacgt aatttgaaa cagcgatat cggttgttg gcgttttcg gaaaactat ttaaaaact aaatgaatt tgttaaatt ttttatata atttatgtt atggatgat tttatagac acgaaaggt agtgatatc aagcgtttt tattgattg tgcatctga taatatgaa caaactgta cataaataa aggaatacg aaataa
