Status
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Gene information
General information
TC011886 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12228718..12237182
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011886
    iBeetle screen iB_06498

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011886 RA
      MATLNPVQW EKPLSLDRQ SPSLHVLEV PLPSLQNPS 
      FLHSQKLRE NLIESRKAF LRNLNEDEK LEPNNEPRK 
      TPTKKISFK IKKTASIYR HNQKMSTIS ASKVAELTM 
      KFNMINEKN SLLEQPKFR KLVKRVNSQ RSLDSDKVT 
      KHSVKNVVT RKPSVKTKP YEDKEIVIV RRKPTIKRK 
      PSRANLEKP VLVTEQNRP TTLPLDEPQ PPSTGTVRA 
      AIEIFERRN SSPVPKPKV PEKPTKLKP DKTDEDLVQ 
      IVNIPAVLP QRRCDSMYE TLNVTKTPL LKPTKSEET 
      IVRPKPNTS FLWSINQDK SPSAKDDWK DLYDFVDPS 
      PQVPPRAAP AKPPTPPKK TFMKPPPEE KIYEELNVS 
      RDEDTISHS YEYCEGDDG YEICAKENI YETLPPPLL 
      PRRQQQEPL PPRPPSRSS YCTIQNPEN VSNCYESIY 
      NPESKSNES TYESIYGCQ IREGGSNRD SLVSSDQQS 
      NSLYGRASL VGWGEDGTN PYSGKATSD LSTSDRSDD 
      WVDMTDNED NDNNNEIII IRERQKAKK TTGWSQQFR 
      EQWSKSPRK LPTDQRTDS DPDHLYERL CTNENTQDD 
      DYDSFDSDS DSEGSFTKV NSHDSGLDI GNSQLPEPP 
      NNQTYVLTR LAATAGKHM RKLRRNWSL TKNDISKSL 
      SRMTKRKSR TDLAKHRKS SPEILQPEE PPPPCDNPP 
      EAEAKTLPR KSFLTKFRR SMSLSAESA SELTSNLDK 
      PKSTFYLTE IDIDKNESE RDSGASLSP VQRNSKVIT 
      RPQSPPPPA PVREKKRST SWYAEVGLF KNSESNAQR 
      RSNTFWYAE VGLYQTGMS TPSTSSAEN SGNTTNVSI 
      KPDDFINQD NSEEYYNLK NGSDYYNDS INSFSSTET 
      KKDQSLTTL ATDAHLRLE DEPLYQFYD AAVLESVCH 
      DGVSDFDSD GYEEVGDNN SESTLSSRP SAMELVTPN 
      KNLLTVSRT LWCEIPEVI QSSVLSTLS THQKKLQEA 
      KFEMITSEA SYLNSLNVL CNHFVKSFE SLDILSKDE 
      LEMLFGKVA DVKNCSEKL ISDLEKCWQ ENILLHGVC 
      DIVQKHAEE NFHVYVPYC ENQILLDDT LRRIKERPN 
      FLEQLKQLE SSSTCQSLT LYSFLMLPM QRITRWPLL 
      VDAVLKRLS PQDSEYLTC QYALATLNK IVSQCNEGA 
      RRKERENEM KKIVTQLEF AKGVPPIEI VSDNRWLIR 
      SGTVTHMQQ RNDEIKLTF GKRFTKVTL HLFLFNDYL 
      FVTKPKSEN LYTVVHYCA RNLVELSST DMLVSLPVK 
      DTQGRHLLF LTILENQND KTVEFLLSC NSESDKERW 
      IEALTPPKS EDPNETLYE CWDCPQVTA IHNYLASQP 
      DELALSRGD VINVLRKMA DGWYHGERI RDGQTGWFP 
      ANYTVEIAN PHVRSRNLK QRYRLLAFS ENYLKTK
    CDS sequence
    • >TC011886 RA
      atggcaacc ttaaatcca gttcagtgg gagaagcct 
      ttgtcgttg gaccgacaa agtccatct ctccacgtc 
      ctggaagtc ccgcttcca agcctccaa aacccgtcg 
      ttcctccac tcacaaaag ctgcgagag aacctcatc 
      gaatcccgc aaggcgttc cttcgcaac ctcaatgaa 
      gacgaaaag ctagaaccc aacaatgaa ccccggaaa 
      acccccacg aaaaaaatc agcttcaaa atcaaaaaa 
      accgcctcg atctaccgc cacaaccag aaaatgtcc 
      acgatcagt gcaagcaaa gtcgccgaa ctcacaatg 
      aagttcaac atgatcaac gagaagaac tccctcctg 
      gaacagccc aagttccgc aagctcgtc aagcgcgtc 
      aacagccaa agaagctta gacagtgat aaagtgacc 
      aagcacagt gttaagaac gtagtgacg cgcaaaccc 
      agcgttaaa acgaaaccc tacgaggac aaagagatt 
      gtcatagtg aggcgcaaa ccgacgatc aaacgcaaa 
      cccagccgt gccaattta gaaaaaccg gtgctagtg 
      accgaacaa aaccgcccc acaaccctc cccctcgac 
      gaaccccaa ccgccgtcc acgggaacc gtccgtgcg 
      gcgatcgaa attttcgaa cgcagaaac tcaagccca 
      gtgccaaaa cccaaagtg cctgaaaaa ccaacgaaa 
      ctaaaaccc gacaagact gacgaagat ttagtacaa 
      atcgttaac attccggcg gtgctgccg caacgaaga 
      tgtgattcc atgtacgaa acgctcaat gttacgaaa 
      acaccactc ctaaaaccg accaagtcg gaggaaacc 
      atcgttagg ccaaaaccc aacacttcc ttcctctgg 
      agcatcaac caggacaag tcgccctcg gccaaagac 
      gactggaag gacctctac gacttcgtc gacccctcc 
      ccacaagtc cccccgagg gccgccccg gcgaaaccc 
      ccaactccc ccgaaaaaa accttcatg aagcctcct 
      cccgaggag aagatttac gaggagttg aatgtgagt 
      cgggacgag gacacgatc agccacagc tacgagtac 
      tgcgaaggg gatgatggg tacgagatc tgtgccaag 
      gagaatatc tacgaaacg ctcccgccg ccgcttcta 
      cccaggagg cagcagcag gaaccgctt ccgccaaga 
      ccaccctcc cggagcagc tattgcacg attcaaaac 
      ccggaaaac gtgtcaaat tgctacgaa tcgatctac 
      aatcccgaa tccaagtcg aatgagagt acttatgag 
      agtatctac gggtgccaa atccgcgag ggtggctcg 
      aatcgcgat tctcttgtg tcctcggac cagcagagt 
      aatagtttg tacgggagg gctagtcta gtggggtgg 
      ggtgaggat gggacgaat ccctacagc ggcaaggcc 
      acaagtgac ttaagcacg agtgaccgg agcgatgat 
      tgggtcgat atgaccgat aatgaggat aatgacaac 
      aacaatgaa atcatcata atccgcgag cgtcaaaag 
      gcgaagaag actaccggt tggtcccag caatttcgg 
      gagcaatgg tctaaatcg ccgcgaaaa ctcccaact 
      gatcagcgt actgacagt gacccggac cacctctac 
      gagagactt tgcaccaat gagaacacc caagacgac 
      gattacgac tccttcgac agtgatagt gattcggaa 
      ggttccttc actaaagtc aattcacat gacagcggc 
      ctggacatc ggcaacagc caactcccc gaaccccca 
      aacaaccaa acctacgtc ctgacccgt ctcgcggcc 
      accgccggc aaacacatg cgcaagctg cgccgcaac 
      tggtcactg accaaaaac gacatcagc aaatcactg 
      agtcggatg accaaacgc aagtcgcgc acagacctc 
      gccaaacac aggaaatct agtcccgaa attctccaa 
      ccggaggag ccgccccct ccttgtgat aaccccccg 
      gaggcggaa gccaagacc ctgcccagg aagagcttc 
      ctgacgaag ttccggagg agcatgtct ctgagtgcc 
      gagtcggcg agcgagctc acctcgaat ttggataag 
      ccgaagagt acgttctac ttgacggaa attgacatc 
      gataagaac gagtctgag agggacagc ggggcgtcg 
      ttatcgccg gtgcagagg aactccaag gtcataacg 
      cggccgcag agcccgccc ccgcccgca ccggtcagg 
      gagaaaaag cggtcgact tcgtggtac gcagaagtg 
      gggctgttt aaaaattcg gaaagcaac gcgcagagg 
      aggagcaat acgttctgg tacgccgaa gtcgggctc 
      taccagact gggatgagc accccgagc acgagttcg 
      gcggaaaat agcggaaat acaaccaat gtttcgatt 
      aaaccggac gattttatc aaccaggat aacagcgag 
      gagtactac aatctcaag aacggctcg gattattac 
      aatgacagt attaacagc tttagcagc acggagacc 
      aagaaagat cagtctttg acgactctg gctactgac 
      gctcacttg aggctggaa gatgagcct ttgtatcag 
      ttttacgac gctgcggtt ttagagtcg gtttgtcac 
      gatggtgtc tccgatttt gattccgac ggctatgaa 
      gaagtcggt gataacaac tccgaatcc actctttcc 
      tcaagacca tcagctatg gaactagtc acacccaac 
      aaaaattta ctgacggtt tcaagaacc ctttggtgt 
      gaaatacca gaagtgata cagtcgtcg gtgttaagc 
      actttgagt acgcatcag aagaagttg caagaagct 
      aagttcgaa atgatcacg tctgaagcc tcctacttg 
      aactccctc aacgttctg tgcaaccac tttgtcaaa 
      agtttcgaa agtttggat attttgtca aaggatgag 
      ctcgaaatg ttgttcgga aaagtcgca gatgtgaaa 
      aactgttcg gagaagctt atatccgac ttggagaag 
      tgttggcag gaaaatatt ctgcttcat ggagtgtgt 
      gatattgtt cagaagcac gctgaggag aatttccac 
      gtgtatgtc ccctactgt gagaatcaa attttgtta 
      gacgacacg ttaaggcgt atcaaggaa cgtccaaat 
      tttctcgaa caattaaaa cagcttgaa tcaagctca 
      acgtgtcag tcactcaca ctttactca tttttaatg 
      ctcccaatg cagaggata acgaggtgg ccactgctt 
      gtggacgcc gtcttgaag aggctctcg ccacaagac 
      tccgaatat ttaacgtgt caatacgcg cttgcaacg 
      ctcaataaa atcgtcagt cagtgcaac gaaggggca 
      agacgtaaa gaacgcgaa aatgaaatg aaaaaaatc 
      gtaacacag ttagagttc gccaagggg gttcccccg 
      attgaaata gtgtcggac aatcggtgg ctcatccgc 
      tccggtact gtaacccac atgcagcaa aggaacgat 
      gagattaag ctgactttt ggaaaacgg ttcacgaaa 
      gtgacgcta cacttgttt ttattcaac gattatttg 
      tttgttaca aaaccgaaa agcgaaaat ttgtacacc 
      gtggtccat tactgcgcc cgcaatttg gtggagttg 
      agcagcacc gacatgttg gtgtccctc ccggtcaaa 
      gacacgcaa ggtcgccac ttgctgttc ctcacaatt 
      ttggaaaat cagaatgac aagactgtt gaatttttg 
      ttgtcttgt aacagtgaa agcgacaag gagcgatgg 
      atcgaggcg ctaacgccc ccaaaatct gaagaccca 
      aacgaaact ctttacgaa tgttgggac tgtccgcaa 
      gtaactgcc attcataat tatctggct agtcaaccc 
      gacgaattg gctttatct cgaggggat gtgattaac 
      gttctccgc aaaatggcc gatggttgg tatcacgga 
      gagagaata agagatggc caaactggc tggttcccc 
      gcgaattat actgtagaa atagcgaac cctcatgtc 
      agatcacgt aatttgaaa cagcgatat cggttgttg 
      gcgttttcg gaaaactat ttaaaaact aaatga
    mRNA sequence
    • >TC011886 RA
      ctagacatt aagcacgtg tgtaaactg ggtttctgt 
      gataatcca ataccagct acaatagct tgtcatcga 
      tctacatgc tgtcatttt gttccagaa ttttgccaa 
      ccatgagac cgcacatgt cgcatttga ataaaaaag 
      tgtagtggc ctcgggacg atggcaacc ttaaatcca 
      gttcagtgg gagaagcct ttgtcgttg gaccgacaa 
      agtccatct ctccacgtc ctggaagtc ccgcttcca 
      agcctccaa aacccgtcg ttcctccac tcacaaaag 
      ctgcgagag aacctcatc gaatcccgc aaggcgttc 
      cttcgcaac ctcaatgaa gacgaaaag ctagaaccc 
      aacaatgaa ccccggaaa acccccacg aaaaaaatc 
      agcttcaaa atcaaaaaa accgcctcg atctaccgc 
      cacaaccag aaaatgtcc acgatcagt gcaagcaaa 
      gtcgccgaa ctcacaatg aagttcaac atgatcaac 
      gagaagaac tccctcctg gaacagccc aagttccgc 
      aagctcgtc aagcgcgtc aacagccaa agaagctta 
      gacagtgat aaagtgacc aagcacagt gttaagaac 
      gtagtgacg cgcaaaccc agcgttaaa acgaaaccc 
      tacgaggac aaagagatt gtcatagtg aggcgcaaa 
      ccgacgatc aaacgcaaa cccagccgt gccaattta 
      gaaaaaccg gtgctagtg accgaacaa aaccgcccc 
      acaaccctc cccctcgac gaaccccaa ccgccgtcc 
      acgggaacc gtccgtgcg gcgatcgaa attttcgaa 
      cgcagaaac tcaagccca gtgccaaaa cccaaagtg 
      cctgaaaaa ccaacgaaa ctaaaaccc gacaagact 
      gacgaagat ttagtacaa atcgttaac attccggcg 
      gtgctgccg caacgaaga tgtgattcc atgtacgaa 
      acgctcaat gttacgaaa acaccactc ctaaaaccg 
      accaagtcg gaggaaacc atcgttagg ccaaaaccc 
      aacacttcc ttcctctgg agcatcaac caggacaag 
      tcgccctcg gccaaagac gactggaag gacctctac 
      gacttcgtc gacccctcc ccacaagtc cccccgagg 
      gccgccccg gcgaaaccc ccaactccc ccgaaaaaa 
      accttcatg aagcctcct cccgaggag aagatttac 
      gaggagttg aatgtgagt cgggacgag gacacgatc 
      agccacagc tacgagtac tgcgaaggg gatgatggg 
      tacgagatc tgtgccaag gagaatatc tacgaaacg 
      ctcccgccg ccgcttcta cccaggagg cagcagcag 
      gaaccgctt ccgccaaga ccaccctcc cggagcagc 
      tattgcacg attcaaaac ccggaaaac gtgtcaaat 
      tgctacgaa tcgatctac aatcccgaa tccaagtcg 
      aatgagagt acttatgag agtatctac gggtgccaa 
      atccgcgag ggtggctcg aatcgcgat tctcttgtg 
      tcctcggac cagcagagt aatagtttg tacgggagg 
      gctagtcta gtggggtgg ggtgaggat gggacgaat 
      ccctacagc ggcaaggcc acaagtgac ttaagcacg 
      agtgaccgg agcgatgat tgggtcgat atgaccgat 
      aatgaggat aatgacaac aacaatgaa atcatcata 
      atccgcgag cgtcaaaag gcgaagaag actaccggt 
      tggtcccag caatttcgg gagcaatgg tctaaatcg 
      ccgcgaaaa ctcccaact gatcagcgt actgacagt 
      gacccggac cacctctac gagagactt tgcaccaat 
      gagaacacc caagacgac gattacgac tccttcgac 
      agtgatagt gattcggaa ggttccttc actaaagtc 
      aattcacat gacagcggc ctggacatc ggcaacagc 
      caactcccc gaaccccca aacaaccaa acctacgtc 
      ctgacccgt ctcgcggcc accgccggc aaacacatg 
      cgcaagctg cgccgcaac tggtcactg accaaaaac 
      gacatcagc aaatcactg agtcggatg accaaacgc 
      aagtcgcgc acagacctc gccaaacac aggaaatct 
      agtcccgaa attctccaa ccggaggag ccgccccct 
      ccttgtgat aaccccccg gaggcggaa gccaagacc 
      ctgcccagg aagagcttc ctgacgaag ttccggagg 
      agcatgtct ctgagtgcc gagtcggcg agcgagctc 
      acctcgaat ttggataag ccgaagagt acgttctac 
      ttgacggaa attgacatc gataagaac gagtctgag 
      agggacagc ggggcgtcg ttatcgccg gtgcagagg 
      aactccaag gtcataacg cggccgcag agcccgccc 
      ccgcccgca ccggtcagg gagaaaaag cggtcgact 
      tcgtggtac gcagaagtg gggctgttt aaaaattcg 
      gaaagcaac gcgcagagg aggagcaat acgttctgg 
      tacgccgaa gtcgggctc taccagact gggatgagc 
      accccgagc acgagttcg gcggaaaat agcggaaat 
      acaaccaat gtttcgatt aaaccggac gattttatc 
      aaccaggat aacagcgag gagtactac aatctcaag 
      aacggctcg gattattac aatgacagt attaacagc 
      tttagcagc acggagacc aagaaagat cagtctttg 
      acgactctg gctactgac gctcacttg aggctggaa 
      gatgagcct ttgtatcag ttttacgac gctgcggtt 
      ttagagtcg gtttgtcac gatggtgtc tccgatttt 
      gattccgac ggctatgaa gaagtcggt gataacaac 
      tccgaatcc actctttcc tcaagacca tcagctatg 
      gaactagtc acacccaac aaaaattta ctgacggtt 
      tcaagaacc ctttggtgt gaaatacca gaagtgata 
      cagtcgtcg gtgttaagc actttgagt acgcatcag 
      aagaagttg caagaagct aagttcgaa atgatcacg 
      tctgaagcc tcctacttg aactccctc aacgttctg 
      tgcaaccac tttgtcaaa agtttcgaa agtttggat 
      attttgtca aaggatgag ctcgaaatg ttgttcgga 
      aaagtcgca gatgtgaaa aactgttcg gagaagctt 
      atatccgac ttggagaag tgttggcag gaaaatatt 
      ctgcttcat ggagtgtgt gatattgtt cagaagcac 
      gctgaggag aatttccac gtgtatgtc ccctactgt 
      gagaatcaa attttgtta gacgacacg ttaaggcgt 
      atcaaggaa cgtccaaat tttctcgaa caattaaaa 
      cagcttgaa tcaagctca acgtgtcag tcactcaca 
      ctttactca tttttaatg ctcccaatg cagaggata 
      acgaggtgg ccactgctt gtggacgcc gtcttgaag 
      aggctctcg ccacaagac tccgaatat ttaacgtgt 
      caatacgcg cttgcaacg ctcaataaa atcgtcagt 
      cagtgcaac gaaggggca agacgtaaa gaacgcgaa 
      aatgaaatg aaaaaaatc gtaacacag ttagagttc 
      gccaagggg gttcccccg attgaaata gtgtcggac 
      aatcggtgg ctcatccgc tccggtact gtaacccac 
      atgcagcaa aggaacgat gagattaag ctgactttt 
      ggaaaacgg ttcacgaaa gtgacgcta cacttgttt 
      ttattcaac gattatttg tttgttaca aaaccgaaa 
      agcgaaaat ttgtacacc gtggtccat tactgcgcc 
      cgcaatttg gtggagttg agcagcacc gacatgttg 
      gtgtccctc ccggtcaaa gacacgcaa ggtcgccac 
      ttgctgttc ctcacaatt ttggaaaat cagaatgac 
      aagactgtt gaatttttg ttgtcttgt aacagtgaa 
      agcgacaag gagcgatgg atcgaggcg ctaacgccc 
      ccaaaatct gaagaccca aacgaaact ctttacgaa 
      tgttgggac tgtccgcaa gtaactgcc attcataat 
      tatctggct agtcaaccc gacgaattg gctttatct 
      cgaggggat gtgattaac gttctccgc aaaatggcc 
      gatggttgg tatcacgga gagagaata agagatggc 
      caaactggc tggttcccc gcgaattat actgtagaa 
      atagcgaac cctcatgtc agatcacgt aatttgaaa 
      cagcgatat cggttgttg gcgttttcg gaaaactat 
      ttaaaaact aaatgaatt tgttaaatt ttttatata 
      atttatgtt atggatgat tttatagac acgaaaggt 
      agtgatatc aagcgtttt tattgattg tgcatctga 
      taatatgaa caaactgta cataaataa aggaatacg 
      aaataa
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