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Gene information
General information
TC011905 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11843135..11848822
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011905
    iBeetle screen iB_06496

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011905 RA
      MVISMYKSV LISLCYLSV FAPEIDANC TIPVILRGK 
      WFSRENNVN NHVEINDRM MTDRGECVD IQDHFHVNY 
      TMLFKNYDC YTCVKIIVR TVNVLEKIE TPCFAVKHY 
      DASVDRVCR NLDNSQQII TLFSENYVP INCRSSLEG 
      VWNFAYQNR FKFTGECRH PDAQIKSCQ TAGSQFLIS 
      NQKFNITYK KCPGMTGTF DGVVEYSCL GAWFVGKNH 
      YFAVANTKE SRDDEKFRC LLKNRDDDL YIGVSITAE 
      CNTLKTVEQ SPERLHIWP IKSEVVEPG CRLPQNFTG 
      EWINTANID ADIVINETH IIETYYPDE GRFRRTIYV 
      CREQRDTRV MMARLTVDG CQKDYVCFD FVPQHHNVI 
      RYRKGIAVI KDDFPTVCS WVQFQNKEK WRYDLYLKR 
      NPVPIKCPL AGKFNFTQK GEVLFETRI LGGVTLSPR 
      PNTYCKQNI SDFSVCDTE QKEIAIDEN YCLSVDHLG 
      RPVDIYSDP DYKMKCVGF WKENLKSYL ITYDALDPY 
      SKYRCWVYQ RADLNRILM SQAIGPFCD LKQDVTSWN 
      HTEGAAVAI EMQEYERER DQCPMHFDD GSNPWQESE 
      DVVQVLTFG FLGTDSGVI TQPFILLFI GGIAILHIL 
      S
    CDS sequence
    • >TC011905 RA
      atggtgata tcaatgtac aaatccgtg cttatctcg 
      ctatgctat ctgtctgtg tttgcccct gaaatcgac 
      gcaaattgc acgataccg gtcattctc agggggaaa 
      tggttctcg cgtgaaaat aacgttaat aaccatgtt 
      gaaatcaac gatcggatg atgaccgat cggggggag 
      tgtgtggac atccaggac cacttccat gtcaattac 
      accatgctt ttcaagaac tacgactgt tacacgtgt 
      gtgaaaata atcgtccgc actgtgaat gtcttggag 
      aagattgaa accccgtgt ttcgccgtg aaacactac 
      gacgcatcg gtggaccgt gtgtgtcgc aatctggac 
      aacagtcag caaattata acgttgttt tcggaaaat 
      tacgtcccg attaactgt cggtcgtcg ctcgaagga 
      gtctggaat tttgcctat cagaacagg tttaagttc 
      acgggggag tgtcgccat ccagacgcc cagattaag 
      tcgtgccaa acggccgga tcgcagttt ttgatttcc 
      aaccaaaag ttcaacatc acgtacaag aagtgtccc 
      gggatgacg gggaccttt gatggggtc gttgagtat 
      agttgtctc ggggcgtgg tttgttggc aaaaaccac 
      tactttgcc gtggccaac acaaaggag tcacgtgac 
      gacgagaaa ttccgctgt ttgctcaaa aatcgggac 
      gatgatttg tacattggt gtctcgatc acggccgag 
      tgtaacacc ctgaagacc gtggagcag agcccggag 
      cggttgcac atatggcca atcaagagc gaggtggtg 
      gagcccggc tgccgcctc ccgcagaac ttcaccggc 
      gagtggatc aacacggcc aatattgac gccgacata 
      gtgataaac gagactcac atcatcgag acgtactac 
      ccggacgag ggtcgtttc cgaaggacg atttacgtg 
      tgccgggaa caaagggac actcgcgtg atgatggcg 
      cggttaaca gtcgacggc tgtcagaaa gactacgtc 
      tgtttcgac ttcgtcccg cagcaccac aacgtgatc 
      aggtaccgg aagggcatc gccgtgatc aaagacgac 
      ttccccacg gtgtgctcg tgggtgcag ttccagaac 
      aaggagaag tggcgctac gatttgtac ctgaagcgc 
      aatcctgtg ccgatcaag tgccctctc gccggaaaa 
      ttcaatttc actcaaaag ggagaagtg ctttttgaa 
      acacgtatt ttgggtggt gtcacgctc agtccacgc 
      cccaacacg tactgcaaa caaaacatt tccgatttt 
      tcggtctgt gataccgaa caaaaagag attgcaatt 
      gacgagaat tattgcctt tcggtggat cacctgggg 
      cggcctgtc gatatttac agtgatccc gattataaa 
      atgaaatgt gtcggcttt tggaaagag aatttaaaa 
      tcgtattta atcacgtat gatgcgctt gatccctat 
      tcgaagtat cgctgttgg gtgtatcag cgcgccgac 
      ttgaacaga attttaatg tcgcaagct atcgggccg 
      ttctgtgat ttgaaacaa gatgtgact tcgtggaat 
      cacacggag ggcgcggct gtcgccata gagatgcaa 
      gagtacgag agagaacgt gaccaatgt cccatgcac 
      tttgacgac ggcagcaat ccatggcag gaatctgaa 
      gacgtcgtc caggttctc acctttgga tttttaggg 
      actgactcg ggtgttatt acccaaccg tttattctt 
      ttatttatc ggcggtata gcgattcta cacattttg 
      agttaa
    mRNA sequence
    • >TC011905 RA
      cattaaagt ctggtgaaa aattttgtc ggaaaacgg 
      acgactttt ctcgtaaca tccccttct gactccagc 
      cgtactact ttaatctgc ccccataat ttacattcc 
      attcacgga cgtctttgt accacatac aggacgaaa 
      taaaaacat atttcatcc caggaaatt caatattat 
      ggctcctct tgcacgagg catttgcat tttatatca 
      gtcatggaa atagcattt tctgcttct ctatttact 
      ttgttttaa caaagtaca aacaatgtt ttcagattc 
      tggttgccg ttgcaggca accaagaat actgtcgtg 
      acgtcacga gtcgattac tcagtggtt ggggcgtgc 
      ctgccctta taattatct catttatgt gataaaatg 
      gtgatatca atgtacaaa tccgtgctt atctcgcta 
      tgctatctg tctgtgttt gcccctgaa atcgacgca 
      aattgcacg ataccggtc attctcagg gggaaatgg 
      ttctcgcgt gaaaataac gttaataac catgttgaa 
      atcaacgat cggatgatg accgatcgg ggggagtgt 
      gtggacatc caggaccac ttccatgtc aattacacc 
      atgcttttc aagaactac gactgttac acgtgtgtg 
      aaaataatc gtccgcact gtgaatgtc ttggagaag 
      attgaaacc ccgtgtttc gccgtgaaa cactacgac 
      gcatcggtg gaccgtgtg tgtcgcaat ctggacaac 
      agtcagcaa attataacg ttgttttcg gaaaattac 
      gtcccgatt aactgtcgg tcgtcgctc gaaggagtc 
      tggaatttt gcctatcag aacaggttt aagttcacg 
      ggggagtgt cgccatcca gacgcccag attaagtcg 
      tgccaaacg gccggatcg cagtttttg atttccaac 
      caaaagttc aacatcacg tacaagaag tgtcccggg 
      atgacgggg acctttgat ggggtcgtt gagtatagt 
      tgtctcggg gcgtggttt gttggcaaa aaccactac 
      tttgccgtg gccaacaca aaggagtca cgtgacgac 
      gagaaattc cgctgtttg ctcaaaaat cgggacgat 
      gatttgtac attggtgtc tcgatcacg gccgagtgt 
      aacaccctg aagaccgtg gagcagagc ccggagcgg 
      ttgcacata tggccaatc aagagcgag gtggtggag 
      cccggctgc cgcctcccg cagaacttc accggcgag 
      tggatcaac acggccaat attgacgcc gacatagtg 
      ataaacgag actcacatc atcgagacg tactacccg 
      gacgagggt cgtttccga aggacgatt tacgtgtgc 
      cgggaacaa agggacact cgcgtgatg atggcgcgg 
      ttaacagtc gacggctgt cagaaagac tacgtctgt 
      ttcgacttc gtcccgcag caccacaac gtgatcagg 
      taccggaag ggcatcgcc gtgatcaaa gacgacttc 
      cccacggtg tgctcgtgg gtgcagttc cagaacaag 
      gagaagtgg cgctacgat ttgtacctg aagcgcaat 
      cctgtgccg atcaagtgc cctctcgcc ggaaaattc 
      aatttcact caaaaggga gaagtgctt tttgaaaca 
      cgtattttg ggtggtgtc acgctcagt ccacgcccc 
      aacacgtac tgcaaacaa aacatttcc gatttttcg 
      gtctgtgat accgaacaa aaagagatt gcaattgac 
      gagaattat tgcctttcg gtggatcac ctggggcgg 
      cctgtcgat atttacagt gatcccgat tataaaatg 
      aaatgtgtc ggcttttgg aaagagaat ttaaaatcg 
      tatttaatc acgtatgat gcgcttgat ccctattcg 
      aagtatcgc tgttgggtg tatcagcgc gccgacttg 
      aacagaatt ttaatgtcg caagctatc gggccgttc 
      tgtgatttg aaacaagat gtgacttcg tggaatcac 
      acggagggc gcggctgtc gccatagag atgcaagag 
      tacgagaga gaacgtgac caatgtccc atgcacttt 
      gacgacggc agcaatcca tggcaggaa tctgaagac 
      gtcgtccag gttctcacc tttggattt ttagggact 
      gactcgggt gttattacc caaccgttt attctttta 
      tttatcggc ggtatagcg attctacac attttgagt 
      taatcgagt aactcaatt attttctgg tggcggagc 
      gggaaaagc tccattcat cgtgttatt ttttcgatt 
      gccaacatt ctatccaag tgttcgatt agaagatat 
      ttactcatt ccgtcttta ttcattgta attagtgat 
      aaaactgtt tatgtggcc ttgaatcgc cttgaactg 
      ttatgcaat ttgcatcat tatgtagtg tacaatttt 
      catctttgt gacaatcga tccgtccat ttatttgat 
      attttatat tttgatccg tccatttat ttgatttct 
      gtattgttt agtgttaaa gcaaattta ctaacgatt 
      gcgcacgat catcatcac tccgctaat agaagtgtc 
      atttatcgt taataaatc gacgtttta tacttaata 
      gtgttaagt caatttgta atattttgt atggtcaga 
      ttaaatatt tgttgagta c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY