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Gene information
General information
TC034884 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11676391..11686338
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC034884
      iBeetle screen iB_06493

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034884 RA
        MDKARLLEN NPLAKTPLN RPLSPSGFR 
ICTLCCYFK ITELTDSRT GGVLNAQLI 
VSFKDGDCN IAEELYITH SPIKVFFPP 
RQVRKNASD LSAKAGPGV GAAACFLLV 
QF
      CDS sequence
      • >TC034884 RA
        atggacaaa gcccgcctg ttggaaaac aatcctctg 
gcaaaaacg ccgctaaac cgccctttg agcccttcc 
ggttttaga atctgtacg ctttgttgt tactttaaa 
atcacagag cttacggat tctcgaact ggtggagta 
ttaaacgcc cagttaatt gtgagcttt aaggatggc 
gattgcaac atagctgaa gagctttac ataactcat 
tcgccgata aaggtcttt tttccgccg agacaagtg 
aggaaaaat gccagtgat ttatccgcg aaagccggc 
cctggagtg ggggcagcg gcgtgcttc ctactcgtc 
caattttaa
      mRNA sequence
      • >TC034884 RA
        ttttgtata atcacattt tgccaagtc atacttttg 
actttttct aaaaagtaa agtgctaat gaaaatact 
tttgggaag acaatcaat tttcggttg caataaaat 
aaatcgact cttttgcac attttcaaa cccaaaatt 
taaagaaaa accttttta cgtagtttc tacgtaaaa 
tgttaatat ctacaaggt gagctttat tgttaacac 
gctttacaa aaaatattt aaaaaaaac gttgaaaaa 
catagcaaa atgttttag tgggacaac aaacaaaat 
aatcagctg atcttttta acaaacaat tcggctaaa 
ttttaatac tattttaat ttttgctcg aaaaactta 
aacatatgt tttccattt tttttttaa agacacctc 
ttaagcaaa actgagtgc gacgtaaat tattcctaa 
gtttaaaaa caataatct ccaaaacca gtcatccaa 
ttacaaaaa ttccctcac gtttatttt aattaccat 
gttattttt attacgcca aagaataaa acataaaat 
tgcctaaca aactttcaa tttttagtg attcagtac 
ctaaataaa aacgtggca tgtgataac gtaaacggc 
tttaggttc aagtttagt tcttagtaa agggctttc 
agaagaaaa cttttataa aaacactcg ccttgtgta 
tatggcaca acatttagc aattcgcaa gactccgcc 
tgtactcaa aagacgaac gaatcgtac aagaatatc 
acacatcaa ttttcaaac agcatcggg gccagtctc 
agcactttt aaactaatc cacgagaat tttttcagt 
tctcctgaa acgaaactt tcacaaaag aaaggtgtc 
gggaaaact gaaaaccgc taccgtatg atgttttac 
tatagaatt aatatcgta attagggtt ctagtactt 
gacatattc gttaataaa aagttcatc taaaaaatg 
gaattaata aaatttgta acgtaagta taggtaagc 
ttaacattg attaattgt aattaacaa tctatatat 
tctaaaaat cacaaattc taattataa gacgatcca 
cgaatgcag agttccccg caatttccc gttttccat 
aatttcatg caaaataat tccatattg tggcgcgga 
aaatgttta tttatgcaa taaaaacca tgcacacgc 
gaagaactt gttgattat ttaaaataa gatttatgt 
aactgctgg cacagattc agaggcaca ctgtatata 
ataagggaa aaaacgtca tttaggccc atttgtaag 
tgctttcgt acaaattat gtcacttgg tgtaccacg 
ctgtaacga tttgtgtaa cgacaacaa atgttatta 
taaataaat catcataaa ccaccaccg agactttga 
ttcattccc cccaaatgg acaaagccc gcctgttgg 
aaaacaatc ctctggcaa aaacgccgc taaaccgcc 
ctttgagcc cttccggtt ttagaatct gtacgcttt 
gttgttact ttaaaatca cagagctta cggattctc 
gaactggtg gagtattaa acgcccagt taattgtga 
gctttaagg atggcgatt gcaacatag ctgaagagc 
tttacataa ctcattcgc cgataaagg tcttttttc 
cgccgagac aagtgagga aaaatgcca gtgatttat 
ccgcgaaag ccggccctg gagtggggg cagcggcgt 
gcttcctac tcgtccaat tttaaatat tgctccgaa 
tttatggaa atttatgtg tttacgtgt tcgaaagtg 
caggaagtg gtcctttcc gcatgtatg caattacat 
tcgagatgc aacttcctg ggggaaaaa tagttatga 
ggtcgagta ataaacagg gcgttacgg ctgtctctg 
caggtgttt gcaatttct taattttta cgaggggca 
ataaagtgt cttaattta c
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY