Status
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Gene information
General information
TC034392
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011928
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11530079..11533957
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034392
    iBeetle screen iB_06489

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034392 RA
      MLIYLFLSV ILSVSFLNA EENKTLFCI VDCDSPGFI 
      ILEPAIGKD STQVDSRSI FLDNSTVTF NTTLPDKWT 
      LISSLNNND NKFHEHVSD QRWETLPNS HSEIAKIMR 
      ALENATVSV FLSVRLNKN ETSLINGSK IGWNLLMKN 
      GSETYLCDN HQTSCTESH LRLEKSVSE LFNNLFSAL 
      WKLHDYTYF YTEKITNTE IYTKRESCS YVRKGCLVM 
      YLAMCKNCN MTVLLNPNT VAYSVNGRN NSIMGTGTW 
      MTVEIDLGN FTDCFSLAA ETIARNGSE DVFWAIGDT 
      IYISTTLSC HKNKRYISL DTTKKLTDF FCETVTNKP 
      LNFSTQLLK PLNKTEKFP LTTTKNDTV PTNKASSFS 
      VVYIIIIAL LITIIITTV VAVLCKRRF WARTPTEAS 
      VAFDSVRLL DIASNSETI DRKDWPLPI KRGEFEDYI 
      HKNCDLRES STELQLQFS ASILTKECN EGKKEANLA 
      KNRYPKILP YDDTRVKLK ANLGDTGTS DYINASYIK 
      GFNSDKEYI VTQNPLENT AVDFWRMIS QEQVEHIVM 
      ISNKEEGVH QYWPQFNKT ESYGGIEVS FTEHDMYEF 
      YDHRVFTIT CFGENRVIN QFHFHAWSK EMLAAYVFA 
      DFINRLLMI PHQSAPVVF HCKAGVGRS ATILLCDIA 
      LRSVAITGN FDLFSITKT MRECRVDMV QNIRQYVLA 
      HLIIYEALN YKDLAIGLD EDCENNFSK YFEGNLVKR 
      LWSSYLENR EWIDEVIMP YHICANYPK LNRYVDPNL 
      LPVEHSRVK LNGLEEADY IHAMYVNDY NQMKNFIVA 
      QQPMRATMT NFWLMVLEK SVTTIINLH ELDPRCGDL 
      CYYPVWNQK FLEINDDIL IKLDSEEQH RNYEKLTII 
      VTRNEQQRK IKLFCIKNW SKIKALPDS TDVVVSLWE 
      AMGDQNPDY PTLICCDDG IRASSFVAC FFYMADKMI 
      HERKCDVVG AVRAVRRTH HNFINFEQF VFLFHCAVA 
      FYKNCVE
    CDS sequence
    • >TC034392 RA
      atgttaatt tatttgttt ctttcggtt attttaagt 
      gtgtcattt ttaaatgct gaagagaat aagacttta 
      ttctgtatc gtggattgt gacagccct ggctttata 
      attttagag cctgccatt ggaaaagac tcgactcaa 
      gtagattct cgctcaatt tttctggac aacagcaca 
      gttacgttt aatactact cttccagat aagtggact 
      cttattagt tccttaaat aataatgac aataaattt 
      catgagcat gtctctgat cagcgttgg gaaacgttg 
      ccaaacagt cacagtgag atcgcaaaa attatgaga 
      gctttggaa aacgccaca gtatcagtt tttttaagc 
      gtgaggcta aataagaac gaaacatca ctaataaat 
      ggcagcaaa atagggtgg aatcttttg atgaaaaat 
      ggctcagaa acgtattta tgtgacaac caccaaaca 
      tcctgtacc gaatcacat ttacggtta gaaaaaagt 
      gtctctgag ttgttcaat aacttattc agtgcctta 
      tggaaactg cacgattac acgtatttt tacactgag 
      aagatcaca aataccgaa atttacaca aaaagggaa 
      agttgtagt tatgtacga aagggctgt cttgtaatg 
      tatctagca atgtgcaag aactgtaac atgacagtt 
      ttgttaaat ccaaacaca gttgcgtat tccgtcaat 
      ggaagaaac aatagcata atgggcact ggcacatgg 
      atgactgta gaaatagac ttgggcaat tttacagat 
      tgtttcagc cttgcagct gaaacaatc gctcgtaat 
      ggctcagaa gacgtattt tgggccatc ggtgacaca 
      atctacatt tcaaccact ctgagttgc cacaaaaat 
      aagcgctac atttcgctc gacaccacg aaaaaattg 
      actgatttt ttttgcgaa actgtgaca aacaagcca 
      cttaacttt agcacccag cttttgaaa cctttgaac 
      aagacagaa aaattccct ctaacaacc accaagaac 
      gatacagtt ccgaccaac aaagctagc tcgtttagt 
      gtagtttac ataattatt attgctcta ctaataaca 
      ataattata acaacagtg gttgcagtt ctttgtaaa 
      agacgattt tgggcacgg acaccaact gaagcaagt 
      gtggcgttt gattcggtg cgcttactt gacatagca 
      tccaactct gaaacaatt gatcgaaaa gactggcct 
      ttgccgatt aaacgcggc gaatttgaa gactacatt 
      cacaaaaac tgtgacttg agagaaagt tccactgag 
      ctccagtta caattttct gcaagtata ttgacgaaa 
      gagtgtaat gaggggaaa aaagaggcg aatttggcc 
      aaaaatcga tacccgaaa atacttcct tacgatgat 
      acccgagtt aagcttaaa gcaaattta ggtgataca 
      ggcactagt gactacatt aacgccagt tatatcaaa 
      ggatttaac agtgataag gaatacatc gtgacccaa 
      aacccgcta gaaaacacc gctgttgac ttctggagg 
      atgatttcg caggaacag gtcgaacac attgtgatg 
      atcagcaac aaagaggag ggggtccac caatattgg 
      ccccaattt aataaaacg gagtcgtac ggaggaatc 
      gaagtttcg ttcacagaa cacgacatg tacgaattc 
      tacgaccac cgtgtgttc accatcacg tgctttggt 
      gaaaacaga gtgataaac caatttcat tttcacgcc 
      tggtcgaaa gagatgctt gcagcgtat gtctttgcc 
      gatttcatc aaccggctc ctgatgatt ccgcaccaa 
      agcgcccct gtggtcttc cactgcaag gccggggtc 
      gggagatca gccactata cttttgtgc gacattgcc 
      ctgcgctca gttgccata actggcaat tttgacttg 
      ttctccatc acgaaaacc atgagggaa tgtcgcgtg 
      gacatggtc cagaatatt aggcaatac gttctcgcc 
      cacttaatt atctacgaa gctttgaat tacaaagat 
      ctggcgatt ggactggac gaggactgc gagaataat 
      ttcagtaaa tacttcgaa ggcaatttg gtgaaaagg 
      ctgtggtcc agttatttg gaaaatagg gagtggatt 
      gatgaagtt ataatgccc taccacatt tgcgcaaac 
      taccccaag ttgaatcgc tacgttgac cctaatctg 
      ctcccagtc gaacacagt cgggttaaa cttaacggt 
      ttggaagaa gctgactac attcacgca atgtatgtg 
      aacgattac aaccaaatg aagaacttt attgtggcc 
      cagcaacca atgagagcc acaatgacc aatttctgg 
      ttgatggtt ttagaaaaa tcggtcact acaattatt 
      aatttacac gaactggac ccgagatgt ggcgatttg 
      tgctattac ccagtgtgg aaccaaaaa tttttagaa 
      ataaacgat gacattttg atcaagttg gattcagag 
      gaacaacac agaaattac gaaaaatta accataatt 
      gtcacaaga aatgagcag caacgcaaa attaaactg 
      ttctgtatc aaaaactgg tcaaaaatt aaagctttg 
      cccgactct accgacgtt gttgtcagt ctgtgggaa 
      gccatgggg gaccaaaat ccagactat cctacacta 
      atctgttgc gatgatggg attagggcg agcagtttt 
      gtggcttgt tttttctac atggccgac aaaatgatt 
      cacgagagg aagtgtgac gtggttggg gccgtgagg 
      gccgttagg cggactcat cacaatttt attaatttc 
      gaacagttt gtttttctt ttccactgt gcagtagca 
      ttttataaa aattgcgtc gagtag
    mRNA sequence
    • >TC034392 RA
      ataagaaca cattttaaa gactgattt atgtttaat 
      ttaacgata aaatcactt atttgatta tttcggcat 
      cttgtaaaa atgttaatt tatttgttt ctttcggtt 
      attttaagt gtgtcattt ttaaatgct gaagagaat 
      aagacttta ttctgtatc gtggattgt gacagccct 
      ggctttata attttagag cctgccatt ggaaaagac 
      tcgactcaa gtagattct cgctcaatt tttctggac 
      aacagcaca gttacgttt aatactact cttccagat 
      aagtggact cttattagt tccttaaat aataatgac 
      aataaattt catgagcat gtctctgat cagcgttgg 
      gaaacgttg ccaaacagt cacagtgag atcgcaaaa 
      attatgaga gctttggaa aacgccaca gtatcagtt 
      tttttaagc gtgaggcta aataagaac gaaacatca 
      ctaataaat ggcagcaaa atagggtgg aatcttttg 
      atgaaaaat ggctcagaa acgtattta tgtgacaac 
      caccaaaca tcctgtacc gaatcacat ttacggtta 
      gaaaaaagt gtctctgag ttgttcaat aacttattc 
      agtgcctta tggaaactg cacgattac acgtatttt 
      tacactgag aagatcaca aataccgaa atttacaca 
      aaaagggaa agttgtagt tatgtacga aagggctgt 
      cttgtaatg tatctagca atgtgcaag aactgtaac 
      atgacagtt ttgttaaat ccaaacaca gttgcgtat 
      tccgtcaat ggaagaaac aatagcata atgggcact 
      ggcacatgg atgactgta gaaatagac ttgggcaat 
      tttacagat tgtttcagc cttgcagct gaaacaatc 
      gctcgtaat ggctcagaa gacgtattt tgggccatc 
      ggtgacaca atctacatt tcaaccact ctgagttgc 
      cacaaaaat aagcgctac atttcgctc gacaccacg 
      aaaaaattg actgatttt ttttgcgaa actgtgaca 
      aacaagcca cttaacttt agcacccag cttttgaaa 
      cctttgaac aagacagaa aaattccct ctaacaacc 
      accaagaac gatacagtt ccgaccaac aaagctagc 
      tcgtttagt gtagtttac ataattatt attgctcta 
      ctaataaca ataattata acaacagtg gttgcagtt 
      ctttgtaaa agacgattt tgggcacgg acaccaact 
      gaagcaagt gtggcgttt gattcggtg cgcttactt 
      gacatagca tccaactct gaaacaatt gatcgaaaa 
      gactggcct ttgccgatt aaacgcggc gaatttgaa 
      gactacatt cacaaaaac tgtgacttg agagaaagt 
      tccactgag ctccagtta caattttct gcaagtata 
      ttgacgaaa gagtgtaat gaggggaaa aaagaggcg 
      aatttggcc aaaaatcga tacccgaaa atacttcct 
      tacgatgat acccgagtt aagcttaaa gcaaattta 
      ggtgataca ggcactagt gactacatt aacgccagt 
      tatatcaaa ggatttaac agtgataag gaatacatc 
      gtgacccaa aacccgcta gaaaacacc gctgttgac 
      ttctggagg atgatttcg caggaacag gtcgaacac 
      attgtgatg atcagcaac aaagaggag ggggtccac 
      caatattgg ccccaattt aataaaacg gagtcgtac 
      ggaggaatc gaagtttcg ttcacagaa cacgacatg 
      tacgaattc tacgaccac cgtgtgttc accatcacg 
      tgctttggt gaaaacaga gtgataaac caatttcat 
      tttcacgcc tggtcgaaa gagatgctt gcagcgtat 
      gtctttgcc gatttcatc aaccggctc ctgatgatt 
      ccgcaccaa agcgcccct gtggtcttc cactgcaag 
      gccggggtc gggagatca gccactata cttttgtgc 
      gacattgcc ctgcgctca gttgccata actggcaat 
      tttgacttg ttctccatc acgaaaacc atgagggaa 
      tgtcgcgtg gacatggtc cagaatatt aggcaatac 
      gttctcgcc cacttaatt atctacgaa gctttgaat 
      tacaaagat ctggcgatt ggactggac gaggactgc 
      gagaataat ttcagtaaa tacttcgaa ggcaatttg 
      gtgaaaagg ctgtggtcc agttatttg gaaaatagg 
      gagtggatt gatgaagtt ataatgccc taccacatt 
      tgcgcaaac taccccaag ttgaatcgc tacgttgac 
      cctaatctg ctcccagtc gaacacagt cgggttaaa 
      cttaacggt ttggaagaa gctgactac attcacgca 
      atgtatgtg aacgattac aaccaaatg aagaacttt 
      attgtggcc cagcaacca atgagagcc acaatgacc 
      aatttctgg ttgatggtt ttagaaaaa tcggtcact 
      acaattatt aatttacac gaactggac ccgagatgt 
      ggcgatttg tgctattac ccagtgtgg aaccaaaaa 
      tttttagaa ataaacgat gacattttg atcaagttg 
      gattcagag gaacaacac agaaattac gaaaaatta 
      accataatt gtcacaaga aatgagcag caacgcaaa 
      attaaactg ttctgtatc aaaaactgg tcaaaaatt 
      aaagctttg cccgactct accgacgtt gttgtcagt 
      ctgtgggaa gccatgggg gaccaaaat ccagactat 
      cctacacta atctgttgc gatgatggg attagggcg 
      agcagtttt gtggcttgt tttttctac atggccgac 
      aaaatgatt cacgagagg aagtgtgac gtggttggg 
      gccgtgagg gccgttagg cggactcat cacaatttt 
      attaatttc gaacagttt gtttttctt ttccactgt 
      gcagtagca ttttataaa aattgcgtc gagtagcaa 
      atttagaaa gagaattcg agtttaggc cttttttag 
      cccaatttt ccccctaac tcataattt atttcgcaa 
      aatatgcga ctagactaa gtttttttc gaagacttt 
      agccagatt tttaggtaa aaacaagga aaaatcacc 
      aaaaagtta aaccaaaag tttaactct tggtgtagg 
      taagcacat tctaataac taggaaaat tctgcacat 
      taggtcaat aatatttgt gttgaagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY