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Gene information
General information
TC012554 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11402226..11404242
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012554
    iBeetle screen iB_06488

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: Mostly between head and thorax.
    larva dorsal pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012554 RA
      MVKLTAELI QNSMQYINP VKDRELDLR GYRIPEVEN 
      LGATGDQFD TIDFSDNDV RKLDGFPYL KRLKCLLFN 
      NNRIVRIGE HLEEYIPNL ETLILTGNH LEELGDIEP 
      LCTLEKLTT VSLLHNPIT SKQHYRLYL IYKLPQLRL 
      LDFRKVTAK EREEARALF KSKKGKEIR KEIGKRAKT 
      FIPGGTGNS IAKSKGLTD EEIAKIREA INKASSLEE 
      VERLQKILQ SGQIPGQEE MNGYQNGQS MEYE
    CDS sequence
    • >TC012554 RA
      atggtgaag ctaacagcc gagctgatt caaaattcc 
      atgcaatac ataaatccg gtaaaagac cgcgagttg 
      gacttacga ggttatcgc atccccgaa gtggaaaat 
      ctgggggcc accggtgac cagttcgac acaattgac 
      ttttcggac aatgatgtg cgcaagttg gatggtttt 
      ccgtacttg aagcggttg aaatgtttg ctttttaac 
      aataaccgc attgtacgt attggggag catttggag 
      gagtacatt cctaatttg gagacgctg attttgact 
      gggaaccac ttggaagag ctgggtgat attgagccg 
      ttgtgcact ttggagaag ctcaccaca gttagtctt 
      ttacataac cctatcact tccaagcag cattacagg 
      ctataccta atttataag ttaccgcag ttaaggttg 
      ctcgatttt cgtaaagtc acagctaag gaacgggaa 
      gaggcgagg gctctgttt aagagtaag aaagggaag 
      gagattaga aaagaaatt gggaaaagg gcaaagacg 
      tttattccc ggaggtaca ggtaacagt attgccaaa 
      agtaaaggc cttacagac gaagaaatt gcgaaaatt 
      cgtgaagct atcaataag gccagttct ttggaagag 
      gttgaacgg ttacagaag attttacag tctggtcaa 
      attcctggt caagaagaa atgaacgga tatcaaaat 
      ggacaaagt atggaatat gaataa
    mRNA sequence
    • >TC012554 RA
      aattacaga aacctgtaa aggtagtcg gtgaaattg 
      atggcttgt gcgttgttt gtttgattg taatttttc 
      gctaaaacc taattatgg tgaagctaa cagccgagc 
      tgattcaaa attccatgc aatacataa atccggtaa 
      aagaccgcg agttggact tacgaggtt atcgcatcc 
      ccgaagtgg aaaatctgg gggccaccg gtgaccagt 
      tcgacacaa ttgactttt cggacaatg atgtgcgca 
      agttggatg gttttccgt acttgaagc ggttgaaat 
      gtttgcttt ttaacaata accgcattg tacgtattg 
      gggagcatt tggaggagt acattccta atttggaga 
      cgctgattt tgactggga accacttgg aagagctgg 
      gtgatattg agccgttgt gcactttgg agaagctca 
      ccacagtta gtcttttac ataacccta tcacttcca 
      agcagcatt acaggctat acctaattt ataagttac 
      cgcagttaa ggttgctcg attttcgta aagtcacag 
      ctaaggaac gggaagagg cgagggctc tgtttaaga 
      gtaagaaag ggaaggaga ttagaaaag aaattggga 
      aaagggcaa agacgttta ttcccggag gtacaggta 
      acagtattg ccaaaagta aaggcctta cagacgaag 
      aaattgcga aaattcgtg aagctatca ataaggcca 
      gttctttgg aagaggttg aacggttac agaagattt 
      tacagtctg gtcaaattc ctggtcaag aagaaatga 
      acggatatc aaaatggac aaagtatgg aatatgaat 
      aagtttgag gtaattttc agtcaggtg tacaattaa 
      ttagtttta tttataagt tccggttga taagagtat 
      tgtactaca ctaaataca aggttgaga agcgagcgg 
      aattaattt ttcattttt tctaataaa gcaatacgt 
      acaaaaact gggtattat ttccaatct ttcccgact 
      gaaacaatg cctttctgg caagattta tctgtttca 
      gaaattaat tgaacgaaa taagctaac aaaaaattt 
      tgttttgca gctaatttt tcgaaaaaa attagtttt 
      tagttttca cagcgagca aattttgac tcagtttaa 
      tgatttttc ctcttcatt aactgaaat aaaacagtt 
      ttttaaatt ttgccgatt ttaaatttc tgtctgtaa 
      acatgtaaa aaataaaaa attgttttt atatcacaa 
      ccgtcttgc gaatacttt ttagtggct actcgtgtc 
      attttaaga gcgccttgg aaattgtgt ctaaagtga 
      ccgaaatac ttttcaaag tgtgtggta atagtggtt 
      ttagaatat ttcaaagtt caaataatt tttttcatt 
      tcaaaatgt tttagttga tttagaatt atttcttta 
      acaattcgc gtcacttat tttatcttt ttaatttta 
      cataatttg tttgttgtt gttttttac aaaaaatga 
      ctagaaaaa ataggtata cttattttt gttgaaatg 
      aaaaataca aaaaagttc attggcatt aatgtacaa 
      tttaaagcg tgaatctaa atttattat ttagatttt 
      tttattttg cacgttatt agttttctc ttctttttt 
      tattcattt actctatat tacagcccc gatagttga 
      ccctgattt caattaaat ttttaaatg attagttgt 
      ttagtcggt cgctgatta atacaaaat agactttag 
      tcgtatctt cgcttttgt aaaaaacaa tttgggtta 
      ttaaatgaa tgatgtgtt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY