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Gene information
General information
TC012553 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11366596..11368206
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012553
    iBeetle screen iB_06487

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012553 RA
      MFQPEFQAV VLAAGRGSR MPEITSGKP KCLLPVGTK 
      PLVWYPLYK LQQSGFTDV ILVVLENHK SEIQATLDK 
      SELEIKIDY FPVSGKEDL GTADSLRLL HDRLKSDVL 
      VISCDFITD FSLKGVLDV FRMHDASVA SLFFHPHGG 
      ELTIPGPKS KHKPERDLV GIDAQTNRL VFLASASDF 
      ESELSLPRS LLKKHTHVK MYSNLVDSH VYVLKNWVV 
      KYLNSQPNF TTIKGELLP HIVKKQLSK PPKGAEGKS 
      IVSKCDSED IFNYAKEDP FSIIIRESS SYNDHIGDS 
      KPTYHGDSI RCYALIAPR DSFGVRVNT LATYWAVNS 
      KVSERWDKI TNGLSLVLR HPKSEIKSS QVDDKCVVW 
      EGAKLHEKT SFKNSVIGA NSEVCSFSR VFNCIVMNN 
      VTIKEKVAL ENCIVSDGV TIEKGCQIK GCLIGSHHL 
      VPENSEHSQ EVLTDSDRL MEF
    CDS sequence
    • >TC012553 RA
      atgtttcag cccgagttc caggccgtg gtcctggcg 
      gccggccgg ggctcccgc atgcccgag atcacctcc 
      ggcaagccc aagtgtctg ctgcccgtg gggacgaag 
      cccctcgtt tggtacccc ttgtacaag ctgcagcag 
      tccggtttc acagacgta attcttgtc gtgttagag 
      aatcacaaa agcgagatt caggccacg cttgacaaa 
      agcgagctt gagatcaaa atcgactac ttccccgtt 
      agcgggaag gaggactta ggcaccgcc gactccctc 
      agactcctc catgaccgg ctaaagtcc gacgttctt 
      gtaatttcg tgcgacttt atcactgat tttagccta 
      aaaggcgtt ttggacgtg ttccgaatg cacgatgcc 
      tcggtcgcg tctttgttc ttccaccct cacgggggc 
      gaattaaca atcccgggc cccaagtcc aaacacaag 
      ccggagcgc gatttggtc gggattgac gcgcaaaca 
      aaccgtttg gtgttttta gcgtccgcg agcgatttc 
      gagagcgaa ttatcgcta ccgcgctct ttgctcaag 
      aaacacact catgttaaa atgtacagc aatttggtc 
      gactcgcac gtgtacgtt ttgaaaaac tgggtggtc 
      aaatatttg aacagccag ccgaacttt acaacaatc 
      aagggcgag ctcttgccc cacattgtc aaaaaacag 
      ctctccaag cccccgaaa ggggccgag ggcaagtca 
      atagtcagt aaatgcgac agcgaggac atttttaat 
      tacgcgaaa gaggaccct ttcagtatt attataagg 
      gagtcctcg tcctacaac gaccatatc ggtgactcc 
      aaacccacg tatcacggt gatagcatc cggtgctac 
      gccttgata gccccccga gactcgttc ggggtgcga 
      gtcaacact ttagcgacg tactgggcc gttaatagc 
      aaagtcagc gaaaggtgg gacaaaatc acaaatggg 
      ctaagcctc gtcctcaga caccccaaa agtgagatt 
      aaatcgagc caagtggac gataagtgt gttgtttgg 
      gagggagcc aaactgcac gagaaaacc tcgtttaag 
      aactcggtt attggggct aacagtgaa gtttgcagt 
      ttttcgagg gtttttaat tgcattgtt atgaataat 
      gtgacgatt aaggagaag gtggcgttg gagaattgt 
      atagtttcg gatggagtg acgatcgag aaggggtgt 
      cagattaag ggctgtttg atcggcagt caccactta 
      gtgcccgaa aattcggaa catagtcaa gaggtgctc 
      acggattcg gaccgcttg atggaattc taa
    mRNA sequence
    • >TC012553 RA
      tgtgatata accacacat ttgttgcaa agttttgtg 
      acatatttt tgtgtgttt ttgcaagaa tgtttcagc 
      ccgagttcc aggccgtgg tcctggcgg ccggccggg 
      gctcccgca tgcccgaga tcacctccg gcaagccca 
      agtgtctgc tgcccgtgg ggacgaagc ccctcgttt 
      ggtacccct tgtacaagc tgcagcagt ccggtttca 
      cagacgtaa ttcttgtcg tgttagaga atcacaaaa 
      gcgagattc aggccacgc ttgacaaaa gcgagcttg 
      agatcaaaa tcgactact tccccgtta gcgggaagg 
      aggacttag gcaccgccg actccctca gactcctcc 
      atgaccggc taaagtccg acgttcttg taatttcgt 
      gcgacttta tcactgatt ttagcctaa aaggcgttt 
      tggacgtgt tccgaatgc acgatgcct cggtcgcgt 
      ctttgttct tccaccctc acgggggcg aattaacaa 
      tcccgggcc ccaagtcca aacacaagc cggagcgcg 
      atttggtcg ggattgacg cgcaaacaa accgtttgg 
      tgtttttag cgtccgcga gcgatttcg agagcgaat 
      tatcgctac cgcgctctt tgctcaaga aacacactc 
      atgttaaaa tgtacagca atttggtcg actcgcacg 
      tgtacgttt tgaaaaact gggtggtca aatatttga 
      acagccagc cgaacttta caacaatca agggcgagc 
      tcttgcccc acattgtca aaaaacagc tctccaagc 
      ccccgaaag gggccgagg gcaagtcaa tagtcagta 
      aatgcgaca gcgaggaca tttttaatt acgcgaaag 
      aggaccctt tcagtatta ttataaggg agtcctcgt 
      cctacaacg accatatcg gtgactcca aacccacgt 
      atcacggtg atagcatcc ggtgctacg ccttgatag 
      ccccccgag actcgttcg gggtgcgag tcaacactt 
      tagcgacgt actgggccg ttaatagca aagtcagcg 
      aaaggtggg acaaaatca caaatgggc taagcctcg 
      tcctcagac accccaaaa gtgagatta aatcgagcc 
      aagtggacg ataagtgtg ttgtttggg agggagcca 
      aactgcacg agaaaacct cgtttaaga actcggtta 
      ttggggcta acagtgaag tttgcagtt tttcgaggg 
      tttttaatt gcattgtta tgaataatg tgacgatta 
      aggagaagg tggcgttgg agaattgta tagtttcgg 
      atggagtga cgatcgaga aggggtgtc agattaagg 
      gctgtttga tcggcagtc accacttag tgcccgaaa 
      attcggaac atagtcaag aggtgctca cggattcgg 
      accgcttga tggaattct aatttttat ttttgtaca 
      taatttacg aataaaata ctaatgtga ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY