Status
Please wait ...
Gene information
General information
TC012545 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11203464..11231438
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012545
    iBeetle screen iB_06486

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    Pupal morphology
    antenna size shortened - Number of animals showing the phenotype: 10

    elytra blistered - Number of animals showing the phenotype: 10

    legs shortened - Number of animals showing the phenotype: 10

    elytra orientation irregular - Number of animals showing the phenotype: 10
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012545 RA
      MRAGLCTVS APLAACSPA AALPPSARF LALHLLHQQ 
      TLYFIVQAK SRVRELYQQ TCLHFAQQG MLDTDLFGL 
      ALICDGEYL FAELDSKLS KYAPKSWRS SHSHGLDAN 
      GRPALAFYF RVQFYVDSP LLLRDETTR HHYYLQLRL 
      NASSGAAGD VAMHLAGLA LQADLGDRT DSTTFRPED 
      YVPPNLRGP AALRTIQVL HRSHRGLSK TEARSRFIT 
      EACQLEEPV NAHVFRLRA SKAEQAPGS ILLAVCAKG 
      LKVCPDNNP PSIFLWSSI GKLSFERKK FEIRTGHEK 
      LTLYTSSDE KSRLLLALC KAAHLFSMA VAPRLSEAR 
      REEEEKQRW DCDQRISVI SSTSSNTTS GIVSDRVHS 
      EDELEIMIT SPPAPSTES LALAHLLDS APASSRTSA 
      ASATAALSA LSLKEDEEE GTKPACSES SAKTAASGN 
      KCAGSQCSS SCSTVVVTP VQNKTKGRR PSTSSSLEL 
      GYSHTAQNS AVSDATCIE LDTREPVYT SGPAPSSHT 
      SGVYTLTSS DQYTLSSDQ FASQSDQVD GPFRDRSNS 
      NVSNSGSFH GDGSDPTDN KQALLSAEE LSDLIVGRY 
      PSCKSVSHT LDSDSDYVT LPSSYQGYV PVPPKRIDS 
      AAEQRFRPP PPPYGAQLV KTPPTEATE FDDQSFVSE 
      LSREPPPYP ENPTPVPPP VYPSEEAAA RFITTRSPS 
      ILTAHASSV GASSASYST PSSVPPIPP KIPRGVPPI 
      NAIITPNNY LDVAASKAS VLVPCTFLP PPPPAPRQP 
      PPPPPALAT VYTSQLSRS QIEQYKQQM YSDVDFVMF 
      PLKEPAISK QEYLEAKLA ALVQTQPVF YRSTSYLGR 
      YASSQNLSD TYVQLPVYG GLEPPPPPL PPNRPRFMR 
      TRSDDNILN SLDAPQPPF RRRLPPPPP VEEKPKCEA 
      KAECDKGLD IRTLREKSK KMDLPLISA LCNDRSLIK 
      QTKAFVVPK HPGEVTPRS LTSTRAKYP VSGLSSTRI 
      SKTTRKMPS ISHRHPGDK LPEIPRAQP NNYVTDPHA 
      KHKTMQSHS
    CDS sequence
    • >TC012545 RA
      atgcgcgcc ggcctgtgc acggtaagc gcccctctg 
      gccgcgtgc agcccggca gcagcgcta cccccgtcg 
      gctcgcttc ttggccctg catttgctg caccaacaa 
      accctctac ttcatcgta caggccaaa tccagggtc 
      agggaactg taccagcag acatgccta cacttcgcc 
      caacagggg atgctcgac accgacttg ttcgggctg 
      gccctcatc tgcgatggc gagtacctg ttcgccgag 
      ctcgatagt aagctctca aagtacgcg cccaaaagt 
      tggcgttcg tcgcacagc catggcttg gacgcaaac 
      gggcgtccg gccctcgcc ttctacttc cgcgtccag 
      ttctacgtg gacagccct ctcctcctg cgcgatgaa 
      acgacccgc caccactac taccttcag ctccgtctt 
      aacgcttct tctggagcc gctggcgac gtcgccatg 
      cacctggcc ggtctggcc ctccaggcg gacctcggc 
      gaccgaacc gactccacc acgttccgg cccgaggac 
      tacgtcccg ccgaaccta cgcgggccc gctgctctg 
      cgcaccatc caggtgctg caccgctcc caccgcggg 
      ctgtccaag accgaggcg cggtcccgg ttcataacg 
      gaggcctgc cagctggag gagcccgtc aacgcgcac 
      gtcttccgc ctgcgggcc tccaaggcc gagcaggcg 
      cccggctcc atcctcctg gccgtctgc gccaagggg 
      ctgaaggtc tgccccgac aacaacccg ccctcgatc 
      ttcctctgg agctccatc ggcaagctc agcttcgag 
      cgcaagaag ttcgagata cggacgggg cacgagaag 
      ctgacgctg tacaccagc agcgacgag aagagccgc 
      ctgctgctg gcgctgtgc aaggcggcg catctgttc 
      agcatggcc gtggcaccg aggctcagc gaggcgcgc 
      cgggaggag gaggagaag cagcgatgg gactgcgat 
      cagaggatt tcggtcatt tcgagcacc tcgtcgaac 
      acgacgtcg gggattgtc agcgaccgg gtgcacagc 
      gaggacgag ttggagatt atgatcacg agtccgccg 
      gcaccttct acggagagt ttggctctg gctcacctg 
      ctggacagt gcgccagct agcagccga acctcggcg 
      gcgtccgcg acggctgcg ctttccgca ctctcgctc 
      aaggaggac gaggaagag ggcaccaag ccggcgtgc 
      agcgagagt tcggccaaa acggccgcg agcgggaac 
      aagtgtgca ggatcgcaa tgcagctcg tcgtgcagt 
      acggtcgtc gtgacgccg gtgcaaaat aaaaccaaa 
      ggccgcaga ccctcaacc agcagcagc ctcgaactc 
      ggttactcc cacacggcc caaaactca gccgtcagt 
      gacgccacc tgcatcgag ctcgacacc cgcgagccc 
      gtctacacc tcgggcccg gcgcccagc agccacacg 
      agcggcgtc tacaccctc acctccagc gaccagtac 
      accctaagc tcggaccag ttcgccagt cagagcgac 
      caagtggac ggccccttc agagacagg tccaactcc 
      aatgtgagc aactcgggc tcgttccac ggcgatggc 
      agcgacccc accgacaac aaacaagcg ctgctgtcg 
      gccgaggaa ctctccgac ctgattgtg ggccgctac 
      cccagctgc aaaagcgtc agccatacg ctggattcg 
      gactctgat tacgtgacc cttccgtcg agttatcag 
      ggctacgtc ccggtgccc cctaagcgg atcgacagc 
      gcggcggag cagagattc aggcccccg ccgccccca 
      tacggggcc caattggtc aagacgccg ccaactgaa 
      gccacagag tttgacgac cagagtttt gtctcggag 
      ttgtcgcgg gagccgccc ccgtacccc gaaaacccg 
      accccggta ccaccccca gtgtacccc tccgaggag 
      gcggcagcc cggttcata accacccga tccccgagt 
      atcctaaca gcgcatgca agcagtgtt ggagcgtct 
      tcagcgagt tattcaacc ccaagttcc gttcctcca 
      atccccccc aaaatccca cgtggggtg cctccaatc 
      aatgccatt atcacacca aataattat ctggacgtt 
      gctgctagc aaagccagt gttttggtc ccttgtacc 
      ttcctccca cctccacct cctgccccc agacaacca 
      ccgccgccg cccccagcc ttggccacg gtctacacc 
      agccagctg tcccgctcc caaatcgaa caatacaaa 
      caacaaatg tacagtgac gttgatttc gtcatgttc 
      ccactgaag gaaccagca atcagcaaa caggaatat 
      ctggaagct aagctggca gcactggtc caaacccaa 
      ccggtgttt taccgcagc acctcctac ttgggccgt 
      tacgcctcc agccagaac ctctccgac acttacgtg 
      cagctcccg gtgtacggg gggctggag ccgccgccg 
      ccgccgctg cccccgaac cgcccgcgg ttcatgcgg 
      acgcgctcc gacgacaat atcttgaat tcgctggac 
      gcgccgcag ccgccgttc cggaggcgg ctgccgccg 
      ccgccgccc gtcgaggag aagcccaag tgcgaggcg 
      aaagccgag tgtgacaag gggttggat attcgtact 
      ttgagggaa aagagcaag aaaatggac ttgccgctg 
      atttccgcg ttgtgcaat gatcggtct ctgataaag 
      cagacgaaa gcgttcgtg gttccgaag catccgggc 
      gaggtgacg ccgcgatcg ttgactagc acgcgagcg 
      aagtaccca gtgtcgggc ctgagcagc accaggatc 
      agcaagacc acgaggaag atgccgtcg atcagccac 
      cggcacccc ggggacaag ctgccggag atcccgagg 
      gcgcagccg aacaattac gtgacggat ccgcacgcc 
      aaacacaaa actatgcag tcacattcg tga
    mRNA sequence
    • >TC012545 RA
      gtggaggtg ccccccgaa cgggccgct tccgcgcgc 
      agaaccgga ctgggcggg cgcattcca gcgatgttt 
      tgccgcgga gtgtacctg accgctcca gagttgaaa 
      tagggaatt tagtcgaag aacaccttt gccggttga 
      tagagacgg cgctctctg gctctgcga aagcacgtg 
      tgtggtgcc tgtgggtgc tctgtgttg tgaattcac 
      ccgaattgg gaattgcgc cggaatggg gctgcggag 
      ggacggttt ttcggattt ttctttcgg cacatggtg 
      agcgggcaa cccccttga agagatgag cgggtcatg 
      cgcgccggc ctgtgcacg gtaagcgcc cctctggcc 
      gcgtgcagc ccggcagca gcgctaccc ccgtcggct 
      cgcttcttg gccctgcat ttgctgcac caacaaacc 
      ctctacttc atcgtacag gccaaatcc agggtcagg 
      gaactgtac cagcagaca tgcctacac ttcgcccaa 
      caggggatg ctcgacacc gacttgttc gggctggcc 
      ctcatctgc gatggcgag tacctgttc gccgagctc 
      gatagtaag ctctcaaag tacgcgccc aaaagttgg 
      cgttcgtcg cacagccat ggcttggac gcaaacggg 
      cgtccggcc ctcgccttc tacttccgc gtccagttc 
      tacgtggac agccctctc ctcctgcgc gatgaaacg 
      acccgccac cactactac cttcagctc cgtcttaac 
      gcttcttct ggagccgct ggcgacgtc gccatgcac 
      ctggccggt ctggccctc caggcggac ctcggcgac 
      cgaaccgac tccaccacg ttccggccc gaggactac 
      gtcccgccg aacctacgc gggcccgct gctctgcgc 
      accatccag gtgctgcac cgctcccac cgcgggctg 
      tccaagacc gaggcgcgg tcccggttc ataacggag 
      gcctgccag ctggaggag cccgtcaac gcgcacgtc 
      ttccgcctg cgggcctcc aaggccgag caggcgccc 
      ggctccatc ctcctggcc gtctgcgcc aaggggctg 
      aaggtctgc cccgacaac aacccgccc tcgatcttc 
      ctctggagc tccatcggc aagctcagc ttcgagcgc 
      aagaagttc gagatacgg acggggcac gagaagctg 
      acgctgtac accagcagc gacgagaag agccgcctg 
      ctgctggcg ctgtgcaag gcggcgcat ctgttcagc 
      atggccgtg gcaccgagg ctcagcgag gcgcgccgg 
      gaggaggag gagaagcag cgatgggac tgcgatcag 
      aggatttcg gtcatttcg agcacctcg tcgaacacg 
      acgtcgggg attgtcagc gaccgggtg cacagcgag 
      gacgagttg gagattatg atcacgagt ccgccggca 
      ccttctacg gagagtttg gctctggct cacctgctg 
      gacagtgcg ccagctagc agccgaacc tcggcggcg 
      tccgcgacg gctgcgctt tccgcactc tcgctcaag 
      gaggacgag gaagagggc accaagccg gcgtgcagc 
      gagagttcg gccaaaacg gccgcgagc gggaacaag 
      tgtgcagga tcgcaatgc agctcgtcg tgcagtacg 
      gtcgtcgtg acgccggtg caaaataaa accaaaggc 
      cgcagaccc tcaaccagc agcagcctc gaactcggt 
      tactcccac acggcccaa aactcagcc gtcagtgac 
      gccacctgc atcgagctc gacacccgc gagcccgtc 
      tacacctcg ggcccggcg cccagcagc cacacgagc 
      ggcgtctac accctcacc tccagcgac cagtacacc 
      ctaagctcg gaccagttc gccagtcag agcgaccaa 
      gtggacggc cccttcaga gacaggtcc aactccaat 
      gtgagcaac tcgggctcg ttccacggc gatggcagc 
      gaccccacc gacaacaaa caagcgctg ctgtcggcc 
      gaggaactc tccgacctg attgtgggc cgctacccc 
      agctgcaaa agcgtcagc catacgctg gattcggac 
      tctgattac gtgaccctt ccgtcgagt tatcagggc 
      tacgtcccg gtgccccct aagcggatc gacagcgcg 
      gcggagcag agattcagg cccccgccg cccccatac 
      ggggcccaa ttggtcaag acgccgcca actgaagcc 
      acagagttt gacgaccag agttttgtc tcggagttg 
      tcgcgggag ccgcccccg taccccgaa aacccgacc 
      ccggtacca cccccagtg tacccctcc gaggaggcg 
      gcagcccgg ttcataacc acccgatcc ccgagtatc 
      ctaacagcg catgcaagc agtgttgga gcgtcttca 
      gcgagttat tcaacccca agttccgtt cctccaatc 
      ccccccaaa atcccacgt ggggtgcct ccaatcaat 
      gccattatc acaccaaat aattatctg gacgttgct 
      gctagcaaa gccagtgtt ttggtccct tgtaccttc 
      ctcccacct ccacctcct gcccccaga caaccaccg 
      ccgccgccc ccagccttg gccacggtc tacaccagc 
      cagctgtcc cgctcccaa atcgaacaa tacaaacaa 
      caaatgtac agtgacgtt gatttcgtc atgttccca 
      ctgaaggaa ccagcaatc agcaaacag gaatatctg 
      gaagctaag ctggcagca ctggtccaa acccaaccg 
      gtgttttac cgcagcacc tcctacttg ggccgttac 
      gcctccagc cagaacctc tccgacact tacgtgcag 
      ctcccggtg tacgggggg ctggagccg ccgccgccg 
      ccgctgccc ccgaaccgc ccgcggttc atgcggacg 
      cgctccgac gacaatatc ttgaattcg ctggacgcg 
      ccgcagccg ccgttccgg aggcggctg ccgccgccg 
      ccgcccgtc gaggagaag cccaagtgc gaggcgaaa 
      gccgagtgt gacaagggg ttggatatt cgtactttg 
      agggaaaag agcaagaaa atggacttg ccgctgatt 
      tccgcgttg tgcaatgat cggtctctg ataaagcag 
      acgaaagcg ttcgtggtt ccgaagcat ccgggcgag 
      gtgacgccg cgatcgttg actagcacg cgagcgaag 
      tacccagtg tcgggcctg agcagcacc aggatcagc 
      aagaccacg aggaagatg ccgtcgatc agccaccgg 
      caccccggg gacaagctg ccggagatc ccgagggcg 
      cagccgaac aattacgtg acggatccg cacgccaaa 
      cacaaaact atgcagtca cattcgtga actgagcgt 
      tccagtgct agagtagag gaattgtta tactcaggg 
      tgccatcat tgcaatatg ttaggttta ggaaggggc 
      cgatctttt gggcaaacg agggctggt tacgcattt 
      tgacgaaat atcggtgcc ttttgccat ccctgtatg 
      tacataaat ggtcatttt ttaataccg cacgtaagt 
      ttacagtac ataatgtat gtttagcaa taatgtata 
      cacttgctc aacttttat tattaaaat aattatatt 
      tttaacttt gttttatgt ttttttatt tatttcaag 
      catccgaaa ttcattcaa agcttcaat caaatgtac 
      ggtgctgcc ttcatttta aaaataaag caatctcca 
      caaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY