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Gene information
General information
TC011945 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11154320..11180712
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011945
    iBeetle screen iB_06485

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    prosternal process potentiallyshortened - Number of animals showing the phenotype: 10


    elytra not closed - Number of animals showing the phenotype: 8


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011945 RA
      MTAPNNKKH ASSTESSSG SSDSSSSSS SSSGSDSSS 
      SSDSESSSS QESTKQQSG SELKKSATI QKNPRKSSD 
      SLKTGTSKS SDPKPAPKP KTQAPKTGK QPAKTTIYS 
      SEDDDKPKP SAKRKSTAK PKSSATITP KAATKPLPK 
      PAVKNVQNK GAKGKEPPK KVVGKNAKN DSANKKKSI 
      FSPENSSES DDAAKKSRP KPAPPKPRP KAAARTVEK 
      PKPVEKKPE KPAPKSKAV VSSASSATS STASSSSSD 
      SESSAESSS TTRKMSDKK ITKKTLVAA KDRNVSDSE 
      TESTKQVTR KLTRSASTR KSKHVLGKS VYSDTDSDT 
      ESTKRSLSR SPVKRAPVT TKGKTKNNK RNDAKSRAE 
      EIIIIEERK CPLEGCNSV GHLSGKYEK HFTLEACPM 
      YHNLTPAQC KEELLERKK REEMRRKAL EFHRKSPRP 
      QPTQEQKQY LQKIRDIHL KFKSEPMEE TKPQNLDKN 
      REPDLSNFV PDYDLKLFR DAQALASEK IEEDLKTQP 
      NTKGTKCIE MGKFEMEVW YQSPYPEDY ARLPKLYIC 
      EYCLRYMKT RTVLQRHVV KCVWRHPPG EEVYRKDKI 
      SVWEVDGKR YKQYCQNLC LLAKFFLDH KTLYYDVEP 
      FLFYVMTIV DTEGCHTVG YFSKEKNSF LNYNVSCIL 
      TLPPYQRQG YGRLLIEFS YLLTKVEGK IGSPEKPLS 
      DLGLISYRS YWKDVLLAY LCSGPGTQL SVKDISQEM 
      AIHSYDIVS TLQALGMMK YWKGKHIIL KKQDVLDEY 
      IERVKKRGS MVKEVDRSC LRWTPPQPA SL
    CDS sequence
    • >TC011945 RA
      atgactgca cccaacaac aagaagcac gccagctca 
      accgagagc tcctccggc tcaagcgac tcttccagc 
      tccagctct tcgtcaagc ggttccgac tcgtcctca 
      tccagcgat agcgaatcg tccagttcg caagaatcc 
      accaaacaa caatccgga tccgaatta aaaaagagc 
      gcaacgatc cagaaaaac ccgcgcaaa agctcagac 
      agcctcaaa accggcact tccaagtca tcagacccc 
      aaaccggcc cctaaaccc aaaacccaa gcccccaaa 
      accgggaaa caaccggcc aaaaccacc atctactcg 
      tcggaagat gacgacaaa cctaaacct agcgcgaag 
      cgcaaaagc acagcaaag cctaaaagc agcgccacg 
      atcacgcct aaagccgcc actaagccg ctgcctaaa 
      ccggccgtt aaaaacgtg caaaataag ggcgccaaa 
      gggaaggaa ccaccgaag aaggtggtc gggaaaaac 
      gcaaagaat gattccgcc aacaagaaa aaaagcatt 
      ttctcgccg gaaaatagc tccgaatcc gacgatgcg 
      gccaagaag agtcggccg aagcccgcg ccacctaaa 
      ccccggccc aaggctgcg gccagaacg gtcgagaag 
      cccaaacct gtggagaag aagcccgaa aaaccggcg 
      cctaaatct aaggccgtg gtcagttct gcgagttcg 
      gctacgtcc tcaaccgcc tctagtagc agctctgat 
      agtgagagt agcgccgaa tcgtccagc actactcgg 
      aaaatgagc gataaaaaa attacgaaa aaaactttg 
      gttgctgcc aaagatcga aatgtgtct gattcggag 
      acggaaagc acgaaacaa gtcactcgg aagttgact 
      cgctccgct agcacccga aaatccaag catgttttg 
      ggcaaaagc gtctattcc gacacggat tcggacacc 
      gaaagcacg aaaagatcg ctgagtcgg tcccccgtc 
      aagcgcgcc ccagtcacc acaaaaggc aaaaccaag 
      aacaataaa cgcaacgac gccaagtcg cgagccgag 
      gaaataata ataatcgag gagcgcaaa tgccccctg 
      gaaggctgc aacagtgtg ggccacctg agcggcaaa 
      tacgagaaa cacttcact ctggaagcc tgccccatg 
      taccacaac ctgacgccg gcccagtgc aaggaagag 
      ctgctggaa cgcaaaaag cgcgaagaa atgcggcgg 
      aaagccctg gagttccac cggaaatcg ccgcggccg 
      cagccaacc caagagcag aagcaatac ttgcagaaa 
      atccgcgac attcactta aaattcaaa tcagagccg 
      atggaagag acaaaaccg caaaatctc gacaaaaac 
      cgcgagccg gatttatcg aatttcgtg cccgattac 
      gatctgaag ctgttcagg gacgcacag gcgttggcg 
      agcgagaag atcgaggag gatttgaag acgcagccg 
      aacaccaaa gggacgaaa tgcatcgaa atgggcaag 
      tttgagatg gaggtctgg taccagagt ccctatcct 
      gaggattac gcgcgcttg ccgaaactt tatatctgc 
      gagtactgc ttgcggtac atgaaaacg aggactgtt 
      ctgcagaga catgtcgtt aagtgtgtc tggagacac 
      ccgcccggg gaggaagtc taccgcaaa gataaaata 
      agtgtgtgg gaagtggac gggaaacgg tataaacag 
      tactgccag aatttgtgt ctgttggcg aagtttttt 
      ctggatcat aagacgctc tattatgac gttgagccg 
      ttcttgttc tatgtcatg acgattgtg gacacggag 
      ggctgtcac accgtggga tatttcagc aaggaaaag 
      aactccttc ctgaattac aacgtatcc tgtatcctg 
      actttgcca ccctatcag cggcaagga tatggaagg 
      cttcttatc gaattcagt tacttgctg acgaaagtc 
      gagggaaag atcggctcc cccgagaag cccttgagc 
      gacttggga ttgatttct tatcgctcg tattggaaa 
      gacgtcctg ctggcgtac ctgtgctct ggaccgggc 
      acgcagtta tcagtgaaa gatatcagc caagagatg 
      gcgatacac tcttatgac atcgtctcg acgctgcaa 
      gcgctcggg atgatgaaa tattggaag ggaaagcac 
      atcatcctc aagaagcag gacgttctg gacgaatac 
      atcgagcgc gtcaagaag cggggctcc atggtcaag 
      gaagtggac cgctcctgt ctcaggtgg acgccgccc 
      cagcccgca tccctctga
    mRNA sequence
    • >TC011945 RA
      caactaacc taaagcaca actgtcagt ggattgtca 
      agtgctttg ttgatattt tttgtaaat aaagcgttt 
      aatcaacaa agggcatgt ccaacatga ctgcaccca 
      acaacaaga agcacgcca gctcaaccg agagctcct 
      ccggctcaa gcgactctt ccagctcca gctcttcgt 
      caagcggtt ccgactcgt cctcatcca gcgatagcg 
      aatcgtcca gttcgcaag aatccacca aacaacaat 
      ccggatccg aattaaaaa agagcgcaa cgatccaga 
      aaaacccgc gcaaaagct cagacagcc tcaaaaccg 
      gcacttcca agtcatcag accccaaac cggccccta 
      aacccaaaa cccaagccc ccaaaaccg ggaaacaac 
      cggccaaaa ccaccatct actcgtcgg aagatgacg 
      acaaaccta aacctagcg cgaagcgca aaagcacag 
      caaagccta aaagcagcg ccacgatca cgcctaaag 
      ccgccacta agccgctgc ctaaaccgg ccgttaaaa 
      acgtgcaaa ataagggcg ccaaaggga aggaaccac 
      cgaagaagg tggtcggga aaaacgcaa agaatgatt 
      ccgccaaca agaaaaaaa gcattttct cgccggaaa 
      atagctccg aatccgacg atgcggcca agaagagtc 
      ggccgaagc ccgcgccac ctaaacccc ggcccaagg 
      ctgcggcca gaacggtcg agaagccca aacctgtgg 
      agaagaagc ccgaaaaac cggcgccta aatctaagg 
      ccgtggtca gttctgcga gttcggcta cgtcctcaa 
      ccgcctcta gtagcagct ctgatagtg agagtagcg 
      ccgaatcgt ccagcacta ctcggaaaa tgagcgata 
      aaaaaatta cgaaaaaaa ctttggttg ctgccaaag 
      atcgaaatg tgtctgatt cggagacgg aaagcacga 
      aacaagtca ctcggaagt tgactcgct ccgctagca 
      cccgaaaat ccaagcatg ttttgggca aaagcgtct 
      attccgaca cggattcgg acaccgaaa gcacgaaaa 
      gatcgctga gtcggtccc ccgtcaagc gcgccccag 
      tcaccacaa aaggcaaaa ccaagaaca ataaacgca 
      acgacgcca agtcgcgag ccgaggaaa taataataa 
      tcgaggagc gcaaatgcc ccctggaag gctgcaaca 
      gtgtgggcc acctgagcg gcaaatacg agaaacact 
      tcactctgg aagcctgcc ccatgtacc acaacctga 
      cgccggccc agtgcaagg aagagctgc tggaacgca 
      aaaagcgcg aagaaatgc ggcggaaag ccctggagt 
      tccaccgga aatcgccgc ggccgcagc caacccaag 
      agcagaagc aatacttgc agaaaatcc gcgacattc 
      acttaaaat tcaaatcag agccgatgg aagagacaa 
      aaccgcaaa atctcgaca aaaaccgcg agccggatt 
      tatcgaatt tcgtgcccg attacgatc tgaagctgt 
      tcagggacg cacaggcgt tggcgagcg agaagatcg 
      aggaggatt tgaagacgc agccgaaca ccaaaggga 
      cgaaatgca tcgaaatgg gcaagtttg agatggagg 
      tctggtacc agagtccct atcctgagg attacgcgc 
      gcttgccga aactttata tctgcgagt actgcttgc 
      ggtacatga aaacgagga ctgttctgc agagacatg 
      tcgttaagt gtgtctgga gacacccgc ccggggagg 
      aagtctacc gcaaagata aaataagtg tgtgggaag 
      tggacggga aacggtata aacagtact gccagaatt 
      tgtgtctgt tggcgaagt tttttctgg atcataaga 
      cgctctatt atgacgttg agccgttct tgttctatg 
      tcatgacga ttgtggaca cggagggct gtcacaccg 
      tgggatatt tcagcaagg aaaagaact ccttcctga 
      attacaacg tatcctgta tcctgactt tgccaccct 
      atcagcggc aaggatatg gaaggcttc ttatcgaat 
      tcagttact tgctgacga aagtcgagg gaaagatcg 
      gctcccccg agaagccct tgagcgact tgggattga 
      tttcttatc gctcgtatt ggaaagacg tcctgctgg 
      cgtacctgt gctctggac cgggcacgc agttatcag 
      tgaaagata tcagccaag agatggcga tacactctt 
      atgacatcg tctcgacgc tgcaagcgc tcgggatga 
      tgaaatatt ggaagggaa agcacatca tcctcaaga 
      agcaggacg ttctggacg aatacatcg agcgcgtca 
      agaagcggg gctccatgg tcaaggaag tggaccgct 
      cctgtctca ggtggacgc cgccccagc ccgcatccc 
      tctgagagc cccccaatt aaactgccg attttggaa 
      gacaatcat aggggcctt gaggggacg acaagctcg 
      tgggcccca caattttac gctgcaccc ccaaagctc 
      accgatttt caaagataa aataaccgc gttaacatg 
      aagggtctg gtggaaaat tttacccct tcattgatg 
      atcatttca taggttaag attgttgat tcaatgtaa 
      ataaaaaca cgtaatctc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY