Status
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Gene information
General information
TC011945 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11154320..11180712
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011945
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06485

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    prosternal process potentiallyshortened - Number of animals showing the phenotype: 10


    elytra not closed - Number of animals showing the phenotype: 8


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011945 RA
      MTAPNNKKH ASSTESSSG SSDSSSSSS SSSGSDSSS 
SSDSESSSS QESTKQQSG SELKKSATI QKNPRKSSD 
SLKTGTSKS SDPKPAPKP KTQAPKTGK QPAKTTIYS 
SEDDDKPKP SAKRKSTAK PKSSATITP KAATKPLPK 
PAVKNVQNK GAKGKEPPK KVVGKNAKN DSANKKKSI 
FSPENSSES DDAAKKSRP KPAPPKPRP KAAARTVEK 
PKPVEKKPE KPAPKSKAV VSSASSATS STASSSSSD 
SESSAESSS TTRKMSDKK ITKKTLVAA KDRNVSDSE 
TESTKQVTR KLTRSASTR KSKHVLGKS VYSDTDSDT 
ESTKRSLSR SPVKRAPVT TKGKTKNNK RNDAKSRAE 
EIIIIEERK CPLEGCNSV GHLSGKYEK HFTLEACPM 
YHNLTPAQC KEELLERKK REEMRRKAL EFHRKSPRP 
QPTQEQKQY LQKIRDIHL KFKSEPMEE TKPQNLDKN 
REPDLSNFV PDYDLKLFR DAQALASEK IEEDLKTQP 
NTKGTKCIE MGKFEMEVW YQSPYPEDY ARLPKLYIC 
EYCLRYMKT RTVLQRHVV KCVWRHPPG EEVYRKDKI 
SVWEVDGKR YKQYCQNLC LLAKFFLDH KTLYYDVEP 
FLFYVMTIV DTEGCHTVG YFSKEKNSF LNYNVSCIL 
TLPPYQRQG YGRLLIEFS YLLTKVEGK IGSPEKPLS 
DLGLISYRS YWKDVLLAY LCSGPGTQL SVKDISQEM 
AIHSYDIVS TLQALGMMK YWKGKHIIL KKQDVLDEY 
IERVKKRGS MVKEVDRSC LRWTPPQPA SL
    CDS sequence
    • >TC011945 RA
      atgactgca cccaacaac aagaagcac gccagctca 
accgagagc tcctccggc tcaagcgac tcttccagc 
tccagctct tcgtcaagc ggttccgac tcgtcctca 
tccagcgat agcgaatcg tccagttcg caagaatcc 
accaaacaa caatccgga tccgaatta aaaaagagc 
gcaacgatc cagaaaaac ccgcgcaaa agctcagac 
agcctcaaa accggcact tccaagtca tcagacccc 
aaaccggcc cctaaaccc aaaacccaa gcccccaaa 
accgggaaa caaccggcc aaaaccacc atctactcg 
tcggaagat gacgacaaa cctaaacct agcgcgaag 
cgcaaaagc acagcaaag cctaaaagc agcgccacg 
atcacgcct aaagccgcc actaagccg ctgcctaaa 
ccggccgtt aaaaacgtg caaaataag ggcgccaaa 
gggaaggaa ccaccgaag aaggtggtc gggaaaaac 
gcaaagaat gattccgcc aacaagaaa aaaagcatt 
ttctcgccg gaaaatagc tccgaatcc gacgatgcg 
gccaagaag agtcggccg aagcccgcg ccacctaaa 
ccccggccc aaggctgcg gccagaacg gtcgagaag 
cccaaacct gtggagaag aagcccgaa aaaccggcg 
cctaaatct aaggccgtg gtcagttct gcgagttcg 
gctacgtcc tcaaccgcc tctagtagc agctctgat 
agtgagagt agcgccgaa tcgtccagc actactcgg 
aaaatgagc gataaaaaa attacgaaa aaaactttg 
gttgctgcc aaagatcga aatgtgtct gattcggag 
acggaaagc acgaaacaa gtcactcgg aagttgact 
cgctccgct agcacccga aaatccaag catgttttg 
ggcaaaagc gtctattcc gacacggat tcggacacc 
gaaagcacg aaaagatcg ctgagtcgg tcccccgtc 
aagcgcgcc ccagtcacc acaaaaggc aaaaccaag 
aacaataaa cgcaacgac gccaagtcg cgagccgag 
gaaataata ataatcgag gagcgcaaa tgccccctg 
gaaggctgc aacagtgtg ggccacctg agcggcaaa 
tacgagaaa cacttcact ctggaagcc tgccccatg 
taccacaac ctgacgccg gcccagtgc aaggaagag 
ctgctggaa cgcaaaaag cgcgaagaa atgcggcgg 
aaagccctg gagttccac cggaaatcg ccgcggccg 
cagccaacc caagagcag aagcaatac ttgcagaaa 
atccgcgac attcactta aaattcaaa tcagagccg 
atggaagag acaaaaccg caaaatctc gacaaaaac 
cgcgagccg gatttatcg aatttcgtg cccgattac 
gatctgaag ctgttcagg gacgcacag gcgttggcg 
agcgagaag atcgaggag gatttgaag acgcagccg 
aacaccaaa gggacgaaa tgcatcgaa atgggcaag 
tttgagatg gaggtctgg taccagagt ccctatcct 
gaggattac gcgcgcttg ccgaaactt tatatctgc 
gagtactgc ttgcggtac atgaaaacg aggactgtt 
ctgcagaga catgtcgtt aagtgtgtc tggagacac 
ccgcccggg gaggaagtc taccgcaaa gataaaata 
agtgtgtgg gaagtggac gggaaacgg tataaacag 
tactgccag aatttgtgt ctgttggcg aagtttttt 
ctggatcat aagacgctc tattatgac gttgagccg 
ttcttgttc tatgtcatg acgattgtg gacacggag 
ggctgtcac accgtggga tatttcagc aaggaaaag 
aactccttc ctgaattac aacgtatcc tgtatcctg 
actttgcca ccctatcag cggcaagga tatggaagg 
cttcttatc gaattcagt tacttgctg acgaaagtc 
gagggaaag atcggctcc cccgagaag cccttgagc 
gacttggga ttgatttct tatcgctcg tattggaaa 
gacgtcctg ctggcgtac ctgtgctct ggaccgggc 
acgcagtta tcagtgaaa gatatcagc caagagatg 
gcgatacac tcttatgac atcgtctcg acgctgcaa 
gcgctcggg atgatgaaa tattggaag ggaaagcac 
atcatcctc aagaagcag gacgttctg gacgaatac 
atcgagcgc gtcaagaag cggggctcc atggtcaag 
gaagtggac cgctcctgt ctcaggtgg acgccgccc 
cagcccgca tccctctga
    mRNA sequence
    • >TC011945 RA
      caactaacc taaagcaca actgtcagt ggattgtca 
agtgctttg ttgatattt tttgtaaat aaagcgttt 
aatcaacaa agggcatgt ccaacatga ctgcaccca 
acaacaaga agcacgcca gctcaaccg agagctcct 
ccggctcaa gcgactctt ccagctcca gctcttcgt 
caagcggtt ccgactcgt cctcatcca gcgatagcg 
aatcgtcca gttcgcaag aatccacca aacaacaat 
ccggatccg aattaaaaa agagcgcaa cgatccaga 
aaaacccgc gcaaaagct cagacagcc tcaaaaccg 
gcacttcca agtcatcag accccaaac cggccccta 
aacccaaaa cccaagccc ccaaaaccg ggaaacaac 
cggccaaaa ccaccatct actcgtcgg aagatgacg 
acaaaccta aacctagcg cgaagcgca aaagcacag 
caaagccta aaagcagcg ccacgatca cgcctaaag 
ccgccacta agccgctgc ctaaaccgg ccgttaaaa 
acgtgcaaa ataagggcg ccaaaggga aggaaccac 
cgaagaagg tggtcggga aaaacgcaa agaatgatt 
ccgccaaca agaaaaaaa gcattttct cgccggaaa 
atagctccg aatccgacg atgcggcca agaagagtc 
ggccgaagc ccgcgccac ctaaacccc ggcccaagg 
ctgcggcca gaacggtcg agaagccca aacctgtgg 
agaagaagc ccgaaaaac cggcgccta aatctaagg 
ccgtggtca gttctgcga gttcggcta cgtcctcaa 
ccgcctcta gtagcagct ctgatagtg agagtagcg 
ccgaatcgt ccagcacta ctcggaaaa tgagcgata 
aaaaaatta cgaaaaaaa ctttggttg ctgccaaag 
atcgaaatg tgtctgatt cggagacgg aaagcacga 
aacaagtca ctcggaagt tgactcgct ccgctagca 
cccgaaaat ccaagcatg ttttgggca aaagcgtct 
attccgaca cggattcgg acaccgaaa gcacgaaaa 
gatcgctga gtcggtccc ccgtcaagc gcgccccag 
tcaccacaa aaggcaaaa ccaagaaca ataaacgca 
acgacgcca agtcgcgag ccgaggaaa taataataa 
tcgaggagc gcaaatgcc ccctggaag gctgcaaca 
gtgtgggcc acctgagcg gcaaatacg agaaacact 
tcactctgg aagcctgcc ccatgtacc acaacctga 
cgccggccc agtgcaagg aagagctgc tggaacgca 
aaaagcgcg aagaaatgc ggcggaaag ccctggagt 
tccaccgga aatcgccgc ggccgcagc caacccaag 
agcagaagc aatacttgc agaaaatcc gcgacattc 
acttaaaat tcaaatcag agccgatgg aagagacaa 
aaccgcaaa atctcgaca aaaaccgcg agccggatt 
tatcgaatt tcgtgcccg attacgatc tgaagctgt 
tcagggacg cacaggcgt tggcgagcg agaagatcg 
aggaggatt tgaagacgc agccgaaca ccaaaggga 
cgaaatgca tcgaaatgg gcaagtttg agatggagg 
tctggtacc agagtccct atcctgagg attacgcgc 
gcttgccga aactttata tctgcgagt actgcttgc 
ggtacatga aaacgagga ctgttctgc agagacatg 
tcgttaagt gtgtctgga gacacccgc ccggggagg 
aagtctacc gcaaagata aaataagtg tgtgggaag 
tggacggga aacggtata aacagtact gccagaatt 
tgtgtctgt tggcgaagt tttttctgg atcataaga 
cgctctatt atgacgttg agccgttct tgttctatg 
tcatgacga ttgtggaca cggagggct gtcacaccg 
tgggatatt tcagcaagg aaaagaact ccttcctga 
attacaacg tatcctgta tcctgactt tgccaccct 
atcagcggc aaggatatg gaaggcttc ttatcgaat 
tcagttact tgctgacga aagtcgagg gaaagatcg 
gctcccccg agaagccct tgagcgact tgggattga 
tttcttatc gctcgtatt ggaaagacg tcctgctgg 
cgtacctgt gctctggac cgggcacgc agttatcag 
tgaaagata tcagccaag agatggcga tacactctt 
atgacatcg tctcgacgc tgcaagcgc tcgggatga 
tgaaatatt ggaagggaa agcacatca tcctcaaga 
agcaggacg ttctggacg aatacatcg agcgcgtca 
agaagcggg gctccatgg tcaaggaag tggaccgct 
cctgtctca ggtggacgc cgccccagc ccgcatccc 
tctgagagc cccccaatt aaactgccg attttggaa 
gacaatcat aggggcctt gaggggacg acaagctcg 
tgggcccca caattttac gctgcaccc ccaaagctc 
accgatttt caaagataa aataaccgc gttaacatg 
aagggtctg gtggaaaat tttacccct tcattgatg 
atcatttca taggttaag attgttgat tcaatgtaa 
ataaaaaca cgtaatctc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY