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Gene information
General information
TC011946 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11124044..11125852
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011946
    iBeetle screen iB_06484

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011946 RA
      MLPVLLVLV LAAVNPCIS DDDISVDKP KPTPTPYHD 
      WDYIVYSQR WPITGCSQW KEKSPKNTC NLPQDNSTW 
      VVHGLWPTK TGQKGPLFC PSALHFDPE QLRPVMKEM 
      QDFWPNVEA NTKPDSFWK HEWGKHGTC AASLPVLNS 
      VINYFKKGL EWNQQYKIS ALLAKSKIV PNPQGYNIS 
      EIYQAVRSA TGKNPIVQC VVDEKKQSL ISEIQICFD 
      KTLDLIHCN VTGALPDGE ILTDCSRKK DVMYFATVP 
      NNASLELEF DEFFGDHEP SIDTCIENY YLERELLKV 
      YRLIKFLMW FTL
    CDS sequence
    • >TC011946 RA
      atgttgccc gttctcctg gtcctggtt ctagcagct 
      gtcaaccct tgcatatcc gatgacgac atttccgtg 
      gataagccc aaaccgacc ccgacccct taccacgac 
      tgggactac atcgtctac agccagcgt tggcccatc 
      acgggctgc tcccagtgg aaggagaag agccccaag 
      aacacgtgc aacctgccc caggacaac tccacctgg 
      gtcgtgcac gggctctgg cccaccaag acggggcaa 
      aaggggccg ctgttctgc ccctcggcc ctgcacttc 
      gaccccgag cagctgcgg cccgtcatg aaggagatg 
      caggatttc tggcccaac gtggaggcg aacaccaag 
      cccgactcg ttctggaag cacgagtgg ggcaagcac 
      ggcacttgc gccgccagt ctcccggtt ttaaacagc 
      gtgatcaac tacttcaaa aaggggcta gagtggaac 
      cagcagtac aaaatctca gctttgctc gcgaaaagc 
      aagatcgtc cccaatccg caagggtac aacatctcg 
      gagatctac caagcggtg agaagcgcc acggggaag 
      aaccccatc gtccagtgc gttgtggac gaaaagaag 
      caatcccta attagcgaa atacaaatt tgcttcgac 
      aaaacgcta gatttgatc cactgtaac gtcactggc 
      gcgctaccc gatggcgag atcttgacc gattgtagc 
      aggaaaaaa gatgtgatg tatttcgcg acagttcct 
      aataacgcc agtttggag ctggagttt gacgagttt 
      tttggagac cacgaacct agcatagac acctgtata 
      gagaactac tacttagaa cgggaattg ttgaaggtt 
      tatcgctta attaaattt ttaatgtgg ttcacattg 
      tag
    mRNA sequence
    • >TC011946 RA
      cgacgcgaa cttggaaaa caaaatccc actcaccga 
      gttattagt aattactca aatattgct ccttttcgg 
      gtttttctt ccaagatta acctcaaac ttttcaaaa 
      acaaacaca aaactggcc acgaaaatg gcgactgcg 
      ccatctaca cagtcactt gttttccaa aatctagaa 
      accaggcga cttgattcg ccaatcgac gccacctat 
      tggccacag aagacacac gacctgacg tgaacgtca 
      aaaatgaca tttcaaata aaacaacaa aggaggaag 
      ataccattc ccaggaaaa tgcatataa tacgataaa 
      taacgcgaa attttaagt gattttagt tccggttca 
      aaattatta tgttgcccg ttctcctgg tcctggttc 
      tagcagctg tcaaccctt gcatatccg atgacgaca 
      tttccgtgg ataagccca aaccgaccc cgacccctt 
      accacgact gggactaca tcgtctaca gccagcgtt 
      ggcccatca cgggctgct cccagtgga aggagaaga 
      gccccaaga acacgtgca acctgcccc aggacaact 
      ccacctggg tcgtgcacg ggctctggc ccaccaaga 
      cggggcaaa aggggccgc tgttctgcc cctcggccc 
      tgcacttcg accccgagc agctgcggc ccgtcatga 
      aggagatgc aggatttct ggcccaacg tggaggcga 
      acaccaagc ccgactcgt tctggaagc acgagtggg 
      gcaagcacg gcacttgcg ccgccagtc tcccggttt 
      taaacagcg tgatcaact acttcaaaa aggggctag 
      agtggaacc agcagtaca aaatctcag ctttgctcg 
      cgaaaagca agatcgtcc ccaatccgc aagggtaca 
      acatctcgg agatctacc aagcggtga gaagcgcca 
      cggggaaga accccatcg tccagtgcg ttgtggacg 
      aaaagaagc aatccctaa ttagcgaaa tacaaattt 
      gcttcgaca aaacgctag atttgatcc actgtaacg 
      tcactggcg cgctacccg atggcgaga tcttgaccg 
      attgtagca ggaaaaaag atgtgatgt atttcgcga 
      cagttccta ataacgcca gtttggagc tggagtttg 
      acgagtttt ttggagacc acgaaccta gcatagaca 
      cctgtatag agaactact acttagaac gggaattgt 
      tgaaggttt atcgcttaa ttaaatttt taatgtggt 
      tcacattgt aggaaaaac taacattct gtaaaatat 
      cacaaagta ggggatttt tttgtataa attgtctag 
      ttgtctaca gttacgtaa ctcaatatt tcaatgaaa 
      gcacattaa ttacgtgtg taaaaatgc aaaacaatt 
      gcacaactc cgcctagtc cctgcatca aaataaggc 
      gtgaaattg caggaactc tacttacgt aatgtttaa 
      ttttatcgt gagtagagt tcttgcagt cagttttat 
      ttttttaag aatcatcaa ttgttcgta atgacgtaa 
      ctattaaat taagaacac aaataaaac gaatgtggg 
      gggaaatga aacatgcct agattttag gaataattg 
      tcatggttt tcagttcac tcgttaatt tgcataaaa 
      ccggtcttt gtaattacg tggtaagtt gtagaaatt 
      catttgttt tgatttaag tctgaacag gagagcatt 
      aattgtaat aattgaaaa taaaatgtg gtcgatggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY