Status
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Gene information
General information
TC012523 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10987625..10991933
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012523
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06479

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012523 RA
      MFINERIVI LYGSQSGNA QDLAERIWR ESKHFHFKS 
TVKSMDDYN VLELVSEQC VIFVCSTTG QGEEPDNMK 
QFWRFLLRR NLPTDSLVN LKYAVFGLG DSSYTKFNF 
AAKRLHKRL LQLGGRSLV PLGLGDDQH DLGYDGAAD 
PWIESLWTK LLLIYPLPT GVQPLPKNL PIVPRWGVQ 
TSSLSQNLA NKPKSIYHP IRQPDDFTV TVIENERTT 
HFDHFQDVR LIKLQTKGQ GYSPGDVVL LRPKNLPWQ 
VETFQNLLK SHNLHFDMT LKITQNDPD IPVPDVLTQ 
ELTFQQLCE EYFDLMSIP RRHIFNILA QITDSELEK 
EKCLEFTTA EGQDDLYTY CNRPKRNIV EVLQDFPHA 
TKNLTKELL FEILPPIKP REFSIASNS KFHQNQIHI 
LLAVVKYKT KLVKERFGL CSNYLAELK PGDQVSARL 
KKGSFRFPA SDVPVIMVG PGTGVAPFR NFIYERVSA 
GAASSGNLL LFFGCRNSN YDFHCKEDF LTLQKTGQL 
NLIPAYSRE QEHKVYVQH KILENCDLV WDFLQRNGQ 
IFIAGNAKL MPQEVRQAF VSVCERKMG KEEAEKFVQ 
MMEKENRYQ TECWS
    CDS sequence
    • >TC012523 RA
      atgttcata aatgagaga attgtgatt ctctacggt 
tctcagtcg ggcaatgcc caagattta gctgaacga 
atttggcga gaatcgaaa catttccac ttcaaaagc 
actgtaaag tctatggac gattacaat gttttagaa 
ctagtcagt gagcagtgt gtgattttt gtttgttca 
accactggc caaggtgag gagccggac aacatgaag 
cagttttgg aggtttctg ttgcggaga aatctacca 
acggactca ctagtcaat ttaaagtat gctgtgttt 
ggattaggg gattctagt tacactaag tttaatttt 
gctgcaaaa cggttacat aagaggttg ctacagttg 
gggggtcgg tctttggtc cccttaggt ttaggtgac 
gaccaacac gatttgggg tatgacggg gccgctgac 
ccttggatc gagtccttg tggacgaaa ttgctactc 
atttacccg ctgccaaca ggggttcag cctctgccc 
aaaaacttg cctattgtg cctcgttgg ggcgtgcaa 
acatcgagt ttgagccaa aacctcgca aacaaacct 
aaatcaatt tatcacccc attcgccaa cctgacgat 
ttcacagtc actgttatc gaaaatgaa agaaccact 
cattttgac cacttccaa gacgtccgg ctcatcaaa 
ctacaaacc aagggccag gggtattcc ccgggcgac 
gtcgtcctt ttacgaccc aaaaacctg ccatggcaa 
gtcgaaacg ttccaaaac ttgctcaaa tcgcacaat 
ttacacttc gacatgacg ctcaaaatc acacaaaac 
gaccccgac attcccgtc cccgacgtt ctaacccaa 
gaactgacg tttcaacaa ctgtgtgaa gaatacttc 
gacctaatg tcaatcccg cgaagacac atcttcaac 
attctggcc caaatcacc gacagcgaa ttggagaaa 
gagaagtgt ctcgaattc acaacggcc gaaggccag 
gacgacctc tacacctac tgcaaccgg cccaagagg 
aacatcgtg gaagtcctt caggacttc ccccacgcc 
accaaaaat ctcaccaaa gagctgctc ttcgagatt 
ttaccacca atcaaaccg cgcgaattt tcaatcgcc 
agcaacagt aaattccac caaaaccaa atccacatt 
cttctggcc gtcgtcaag tacaaaact aagttagtg 
aaggaacgg ttcggccta tgttcgaat tatttagcc 
gagttgaag ccgggcgat caagtgagc gcgaggctt 
aagaagggg agtttccgt ttcccggca tcggacgtc 
ccggtgatc atggtcggc cctggcacc ggcgtcgcc 
cccttccgg aacttcatc tacgagcgc gttagcgca 
ggtgcggcc tctagtggc aacctgttg ttatttttc 
gggtgtcgc aactcaaat tacgatttt cattgcaag 
gaggatttc ctcactttg caaaaaacg ggtcaatta 
aacctgatc ccggcctat tccagggag caggagcac 
aaagtttac gttcagcac aaaatcctg gaaaattgt 
gacctagtg tgggacttt ttgcaacgc aatggccaa 
attttcata gccgggaat gcgaagctg atgccacag 
gaggtgagg caagcgttt gtttccgtt tgtgagagg 
aaaatgggc aaggaagag gccgagaag tttgttcaa 
atgatggag aaagagaat aggtaccag acggagtgc 
tggtcgtga
    mRNA sequence
    • >TC012523 RA
      agtcaatcg tcaaattcc caatgtcaa aaacacgcg 
ttgtgaggt taggtcagt gcggttacg tcagaatga 
gtgaaaaaa aataatttt taaagtaag tgtaggcag 
tttttagga ttcattgca ttttttgag ttatcaagc 
agtattgat atcatgttc ataaatgag agaattgtg 
attctctac ggttctcag tcgggcaat gcccaagat 
ttagctgaa cgaatttgg cgagaatcg aaacatttc 
cacttcaaa agcactgta aagtctatg gacgattac 
aatgtttta gaactagtc agtgagcag tgtgtgatt 
tttgtttgt tcaaccact ggccaaggt gaggagccg 
gacaacatg aagcagttt tggaggttt ctgttgcgg 
agaaatcta ccaacggac tcactagtc aatttaaag 
tatgctgtg tttggatta ggggattct agttacact 
aagtttaat tttgctgca aaacggtta cataagagg 
ttgctacag ttggggggt cggtctttg gtcccctta 
ggtttaggt gacgaccaa cacgatttg gggtatgac 
ggggccgct gacccttgg atcgagtcc ttgtggacg 
aaattgcta ctcatttac ccgctgcca acaggggtt 
cagcctctg cccaaaaac ttgcctatt gtgcctcgt 
tggggcgtg caaacatcg agtttgagc caaaacctc 
gcaaacaaa cctaaatca atttatcac cccattcgc 
caacctgac gatttcaca gtcactgtt atcgaaaat 
gaaagaacc actcatttt gaccacttc caagacgtc 
cggctcatc aaactacaa accaagggc caggggtat 
tccccgggc gacgtcgtc cttttacga cccaaaaac 
ctgccatgg caagtcgaa acgttccaa aacttgctc 
aaatcgcac aatttacac ttcgacatg acgctcaaa 
atcacacaa aacgacccc gacattccc gtccccgac 
gttctaacc caagaactg acgtttcaa caactgtgt 
gaagaatac ttcgaccta atgtcaatc ccgcgaaga 
cacatcttc aacattctg gcccaaatc accgacagc 
gaattggag aaagagaag tgtctcgaa ttcacaacg 
gccgaaggc caggacgac ctctacacc tactgcaac 
cggcccaag aggaacatc gtggaagtc cttcaggac 
ttcccccac gccaccaaa aatctcacc aaagagctg 
ctcttcgag attttacca ccaatcaaa ccgcgcgaa 
ttttcaatc gccagcaac agtaaattc caccaaaac 
caaatccac attcttctg gccgtcgtc aagtacaaa 
actaagtta gtgaaggaa cggttcggc ctatgttcg 
aattattta gccgagttg aagccgggc gatcaagtg 
agcgcgagg cttaagaag gggagtttc cgtttcccg 
gcatcggac gtcccggtg atcatggtc ggccctggc 
accggcgtc gcccccttc cggaacttc atctacgag 
cgcgttagc gcaggtgcg gcctctagt ggcaacctg 
ttgttattt ttcgggtgt cgcaactca aattacgat 
tttcattgc aaggaggat ttcctcact ttgcaaaaa 
acgggtcaa ttaaacctg atcccggcc tattccagg 
gagcaggag cacaaagtt tacgttcag cacaaaatc 
ctggaaaat tgtgaccta gtgtgggac tttttgcaa 
cgcaatggc caaattttc atagccggg aatgcgaag 
ctgatgcca caggaggtg aggcaagcg tttgtttcc 
gtttgtgag aggaaaatg ggcaaggaa gaggccgag 
aagtttgtt caaatgatg gagaaagag aataggtac 
cagacggag tgctggtcg tgatgtttt agcccaatt 
ttgttgtgt ttgagattt tttaataaa cgttttgtt 
actat
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