Status
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Gene information
General information
TC012523 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10987625..10991933
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012523
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06479

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012523 RA
      MFINERIVI LYGSQSGNA QDLAERIWR ESKHFHFKS 
      TVKSMDDYN VLELVSEQC VIFVCSTTG QGEEPDNMK 
      QFWRFLLRR NLPTDSLVN LKYAVFGLG DSSYTKFNF 
      AAKRLHKRL LQLGGRSLV PLGLGDDQH DLGYDGAAD 
      PWIESLWTK LLLIYPLPT GVQPLPKNL PIVPRWGVQ 
      TSSLSQNLA NKPKSIYHP IRQPDDFTV TVIENERTT 
      HFDHFQDVR LIKLQTKGQ GYSPGDVVL LRPKNLPWQ 
      VETFQNLLK SHNLHFDMT LKITQNDPD IPVPDVLTQ 
      ELTFQQLCE EYFDLMSIP RRHIFNILA QITDSELEK 
      EKCLEFTTA EGQDDLYTY CNRPKRNIV EVLQDFPHA 
      TKNLTKELL FEILPPIKP REFSIASNS KFHQNQIHI 
      LLAVVKYKT KLVKERFGL CSNYLAELK PGDQVSARL 
      KKGSFRFPA SDVPVIMVG PGTGVAPFR NFIYERVSA 
      GAASSGNLL LFFGCRNSN YDFHCKEDF LTLQKTGQL 
      NLIPAYSRE QEHKVYVQH KILENCDLV WDFLQRNGQ 
      IFIAGNAKL MPQEVRQAF VSVCERKMG KEEAEKFVQ 
      MMEKENRYQ TECWS
    CDS sequence
    • >TC012523 RA
      atgttcata aatgagaga attgtgatt ctctacggt 
      tctcagtcg ggcaatgcc caagattta gctgaacga 
      atttggcga gaatcgaaa catttccac ttcaaaagc 
      actgtaaag tctatggac gattacaat gttttagaa 
      ctagtcagt gagcagtgt gtgattttt gtttgttca 
      accactggc caaggtgag gagccggac aacatgaag 
      cagttttgg aggtttctg ttgcggaga aatctacca 
      acggactca ctagtcaat ttaaagtat gctgtgttt 
      ggattaggg gattctagt tacactaag tttaatttt 
      gctgcaaaa cggttacat aagaggttg ctacagttg 
      gggggtcgg tctttggtc cccttaggt ttaggtgac 
      gaccaacac gatttgggg tatgacggg gccgctgac 
      ccttggatc gagtccttg tggacgaaa ttgctactc 
      atttacccg ctgccaaca ggggttcag cctctgccc 
      aaaaacttg cctattgtg cctcgttgg ggcgtgcaa 
      acatcgagt ttgagccaa aacctcgca aacaaacct 
      aaatcaatt tatcacccc attcgccaa cctgacgat 
      ttcacagtc actgttatc gaaaatgaa agaaccact 
      cattttgac cacttccaa gacgtccgg ctcatcaaa 
      ctacaaacc aagggccag gggtattcc ccgggcgac 
      gtcgtcctt ttacgaccc aaaaacctg ccatggcaa 
      gtcgaaacg ttccaaaac ttgctcaaa tcgcacaat 
      ttacacttc gacatgacg ctcaaaatc acacaaaac 
      gaccccgac attcccgtc cccgacgtt ctaacccaa 
      gaactgacg tttcaacaa ctgtgtgaa gaatacttc 
      gacctaatg tcaatcccg cgaagacac atcttcaac 
      attctggcc caaatcacc gacagcgaa ttggagaaa 
      gagaagtgt ctcgaattc acaacggcc gaaggccag 
      gacgacctc tacacctac tgcaaccgg cccaagagg 
      aacatcgtg gaagtcctt caggacttc ccccacgcc 
      accaaaaat ctcaccaaa gagctgctc ttcgagatt 
      ttaccacca atcaaaccg cgcgaattt tcaatcgcc 
      agcaacagt aaattccac caaaaccaa atccacatt 
      cttctggcc gtcgtcaag tacaaaact aagttagtg 
      aaggaacgg ttcggccta tgttcgaat tatttagcc 
      gagttgaag ccgggcgat caagtgagc gcgaggctt 
      aagaagggg agtttccgt ttcccggca tcggacgtc 
      ccggtgatc atggtcggc cctggcacc ggcgtcgcc 
      cccttccgg aacttcatc tacgagcgc gttagcgca 
      ggtgcggcc tctagtggc aacctgttg ttatttttc 
      gggtgtcgc aactcaaat tacgatttt cattgcaag 
      gaggatttc ctcactttg caaaaaacg ggtcaatta 
      aacctgatc ccggcctat tccagggag caggagcac 
      aaagtttac gttcagcac aaaatcctg gaaaattgt 
      gacctagtg tgggacttt ttgcaacgc aatggccaa 
      attttcata gccgggaat gcgaagctg atgccacag 
      gaggtgagg caagcgttt gtttccgtt tgtgagagg 
      aaaatgggc aaggaagag gccgagaag tttgttcaa 
      atgatggag aaagagaat aggtaccag acggagtgc 
      tggtcgtga
    mRNA sequence
    • >TC012523 RA
      agtcaatcg tcaaattcc caatgtcaa aaacacgcg 
      ttgtgaggt taggtcagt gcggttacg tcagaatga 
      gtgaaaaaa aataatttt taaagtaag tgtaggcag 
      tttttagga ttcattgca ttttttgag ttatcaagc 
      agtattgat atcatgttc ataaatgag agaattgtg 
      attctctac ggttctcag tcgggcaat gcccaagat 
      ttagctgaa cgaatttgg cgagaatcg aaacatttc 
      cacttcaaa agcactgta aagtctatg gacgattac 
      aatgtttta gaactagtc agtgagcag tgtgtgatt 
      tttgtttgt tcaaccact ggccaaggt gaggagccg 
      gacaacatg aagcagttt tggaggttt ctgttgcgg 
      agaaatcta ccaacggac tcactagtc aatttaaag 
      tatgctgtg tttggatta ggggattct agttacact 
      aagtttaat tttgctgca aaacggtta cataagagg 
      ttgctacag ttggggggt cggtctttg gtcccctta 
      ggtttaggt gacgaccaa cacgatttg gggtatgac 
      ggggccgct gacccttgg atcgagtcc ttgtggacg 
      aaattgcta ctcatttac ccgctgcca acaggggtt 
      cagcctctg cccaaaaac ttgcctatt gtgcctcgt 
      tggggcgtg caaacatcg agtttgagc caaaacctc 
      gcaaacaaa cctaaatca atttatcac cccattcgc 
      caacctgac gatttcaca gtcactgtt atcgaaaat 
      gaaagaacc actcatttt gaccacttc caagacgtc 
      cggctcatc aaactacaa accaagggc caggggtat 
      tccccgggc gacgtcgtc cttttacga cccaaaaac 
      ctgccatgg caagtcgaa acgttccaa aacttgctc 
      aaatcgcac aatttacac ttcgacatg acgctcaaa 
      atcacacaa aacgacccc gacattccc gtccccgac 
      gttctaacc caagaactg acgtttcaa caactgtgt 
      gaagaatac ttcgaccta atgtcaatc ccgcgaaga 
      cacatcttc aacattctg gcccaaatc accgacagc 
      gaattggag aaagagaag tgtctcgaa ttcacaacg 
      gccgaaggc caggacgac ctctacacc tactgcaac 
      cggcccaag aggaacatc gtggaagtc cttcaggac 
      ttcccccac gccaccaaa aatctcacc aaagagctg 
      ctcttcgag attttacca ccaatcaaa ccgcgcgaa 
      ttttcaatc gccagcaac agtaaattc caccaaaac 
      caaatccac attcttctg gccgtcgtc aagtacaaa 
      actaagtta gtgaaggaa cggttcggc ctatgttcg 
      aattattta gccgagttg aagccgggc gatcaagtg 
      agcgcgagg cttaagaag gggagtttc cgtttcccg 
      gcatcggac gtcccggtg atcatggtc ggccctggc 
      accggcgtc gcccccttc cggaacttc atctacgag 
      cgcgttagc gcaggtgcg gcctctagt ggcaacctg 
      ttgttattt ttcgggtgt cgcaactca aattacgat 
      tttcattgc aaggaggat ttcctcact ttgcaaaaa 
      acgggtcaa ttaaacctg atcccggcc tattccagg 
      gagcaggag cacaaagtt tacgttcag cacaaaatc 
      ctggaaaat tgtgaccta gtgtgggac tttttgcaa 
      cgcaatggc caaattttc atagccggg aatgcgaag 
      ctgatgcca caggaggtg aggcaagcg tttgtttcc 
      gtttgtgag aggaaaatg ggcaaggaa gaggccgag 
      aagtttgtt caaatgatg gagaaagag aataggtac 
      cagacggag tgctggtcg tgatgtttt agcccaatt 
      ttgttgtgt ttgagattt tttaataaa cgttttgtt 
      actat
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