Status
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Gene information
General information
TC032111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8596037..8598138
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032111
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06477

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Adult morphology
    elytra shortened - Number of animals showing the phenotype: 10


    elytra not closed - Number of animals showing the phenotype: 10


    elytra surface irregular - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032111 RA
      MIFTATFLT LCLKLLLIP AYRSTDFEV HRNWMAITH 
      SLPLKQWYY ENTSEWTLD YPPLFAYFE YLLSLIASC 
      FDKKMLIVQ NLNYASDET ILFQRLSVI FTDLVFAYG 
      THKCCSSIQ KSWRTDVVL PILLITNCG LIMVDHIHF 
      QYNGILYGI LLLSLAYAI QGRYLLSGF WFAVLLNMK 
      HIYIYLAPA YFVFFLKNY CLNSKHSKF QIKNFIMLG 
      STVLAVFLA TFLPFHNQL GQVFARLFP FKRGLCHAY 
      WAPNFWALY NTLDKAAFF IMSKMGFLA KKSSVASMT 
      GGLVQEFSH EVLPNVTPL VTMILTGVV MLPALIKLW 
      SLNKHPSNF LRCIVLCAL SSFLFGWHV HEKAILMAI 
      IPLSILSIM DLSDAKTFI LLGIVGHYS LFPLLFPSS 
      LLFVQGLPV RRPQLFVSF VKLH
    CDS sequence
    • >TC032111 RA
      atgattttc acggccact tttctcacg ttatgtctc 
      aagctgctc ctgataccg gcttaccgg tctacagac 
      ttcgaagtt cacagaaac tggatggct atcacccac 
      agtttacct ttaaaacaa tggtattat gaaaatacg 
      tccgagtgg acccttgat tatccaccc cttttcgcc 
      tattttgaa tatttgttg tctttaatt gcgtcctgt 
      tttgataag aagatgctg atagtacaa aacctgaac 
      tatgcgtct gatgagacg attttgttt caaagattg 
      tctgtgatt ttcactgat ctagtgttt gcttacgga 
      acacacaag tgctgcagc tccattcaa aaaagttgg 
      aggaccgac gttgttctc cctattttg ctcatcaca 
      aactgtggc ttaatcatg gtggatcac atacatttc 
      cagtacaat ggaattttg tatggcatt ctgttatta 
      tcactcgca tatgcaata caggggaga tatttatta 
      tcgggattt tggttcgca gtccttttg aacatgaag 
      cacatttac atttattta gcaccggct tatttcgtc 
      ttcttcctc aaaaactac tgtctcaac tctaaacat 
      tccaaattc caaatcaaa aatttcatc atgttggga 
      tccacagtc ttggctgtt tttctcgca acgtttcta 
      ccattccac aaccaactg ggccaagtt ttcgcgcgg 
      ttgtttccc ttcaaaagg ggcctctgc cacgcgtat 
      tgggccccc aatttctgg gccctttac aacactctt 
      gataaagca gcatttttc ataatgtca aagatgggc 
      tttttggct aagaaaagc tccgtggcg tcgatgact 
      ggggggttg gtgcaggaa ttttcgcac gaagtcttg 
      ccaaatgtg acaccactg gtgacaatg attctgacg 
      ggggttgtg atgttacca gccttaata aaactgtgg 
      agtcttaat aaacaccca tcgaatttt ttaagatgc 
      attgtttta tgtgcgctg tcgtcgttt ttattcgga 
      tggcatgtg catgaaaaa gctattttg atggcaatc 
      attcctttg agcattttg tcaataatg gatttgagt 
      gacgccaaa acttttatt ttattagga atcgtcgga 
      cattattcc ttgttccca ctccttttt cctagttct 
      ctacttttt gtccaaggt ttacccgtt cgcaggcca 
      caattattc gtttccttt gttaaactg cactga
    mRNA sequence
    • >TC032111 RA
      gtgttgaca gttacataa cctcaacat ctagtcgtt 
      gcaatattt tctgtgata gtgatatga taacatcca 
      gtggtaaac accttccaa aaactccat ccctataat 
      gattttcac ggccacttt tctcacgtt atgtctcaa 
      gctgctcct gataccggc ttaccggtc tacagactt 
      cgaagttca cagaaactg gatggctat cacccacag 
      tttaccttt aaaacaatg gtattatga aaatacgtc 
      cgagtggac ccttgatta tccacccct tttcgccta 
      ttttgaata tttgttgtc tttaattgc gtcctgttt 
      tgataagaa gatgctgat agtacaaaa cctgaacta 
      tgcgtctga tgagacgat tttgtttca aagattgtc 
      tgtgatttt cactgatct agtgtttgc ttacggaac 
      acacaagtg ctgcagctc cattcaaaa aagttggag 
      gaccgacgt tgttctccc tattttgct catcacaaa 
      ctgtggctt aatcatggt ggatcacat acatttcca 
      gtacaatgg aattttgta tggcattct gttattatc 
      actcgcata tgcaataca ggggagata tttattatc 
      gggattttg gttcgcagt ccttttgaa catgaagca 
      catttacat ttatttagc accggctta tttcgtctt 
      cttcctcaa aaactactg tctcaactc taaacattc 
      caaattcca aatcaaaaa tttcatcat gttgggatc 
      cacagtctt ggctgtttt tctcgcaac gtttctacc 
      attccacaa ccaactggg ccaagtttt cgcgcggtt 
      gtttccctt caaaagggg cctctgcca cgcgtattg 
      ggcccccaa tttctgggc cctttacaa cactcttga 
      taaagcagc atttttcat aatgtcaaa gatgggctt 
      tttggctaa gaaaagctc cgtggcgtc gatgactgg 
      ggggttggt gcaggaatt ttcgcacga agtcttgcc 
      aaatgtgac accactggt gacaatgat tctgacggg 
      ggttgtgat gttaccagc cttaataaa actgtggag 
      tcttaataa acacccatc gaatttttt aagatgcat 
      tgttttatg tgcgctgtc gtcgttttt attcggatg 
      gcatgtgca tgaaaaagc tattttgat ggcaatcat 
      tcctttgag cattttgtc aataatgga tttgagtga 
      cgccaaaac ttttatttt attaggaat cgtcggaca 
      ttattcctt gttcccact cctttttcc tagttctct 
      actttttgt ccaaggttt acccgttcg caggccaca 
      attattcgt ttcctttgt taaactgca ctgaaagta 
      tttatattt tagggttta gtgtcgttt tcatgtatg 
      aaaattttt tacaccagt ttttaggcc taggcgcga 
      aattaccgt acttacctc ttctactca catcgttgt 
      attgttctc taggcgtta tctactcgt ggatgcgat 
      tttatatca attttttaa caagaacta gtgttgtac 
      aaacaaagc caaataaaa aggtacaat aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY