| General information | |||
| TC016289 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 10244782..10252460 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC016289 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06476 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
- >TC016289 RA
MGDNDPPVK RYRRDEKDQ EKSDSDDNY VPYVPVKER KKQKLLKLG RLGQLKESE LNKPKSSSE NETNDDEEE DEVWGRKNN ISLLDQHTE LKKLAEAKK VSAVERQLK KEEEILEIV AEKKALMGV SELAKGIQY SDPIKTSWR PPRYVLAMP ENRHEKIRN ELRILVEGE DIPPPLKTF REMKLHEGI ISGLKEKKI KKPTPIQIQ GIPTVLSGR DMIGIAFTG SGKTLVFVL PLIMFCLEQ EVKLPFIKN EGPYGLIIC PSRELAKQT FDIIQHFCN SLRKHGMPE IRTSLAIGG VPVSEAVDV IQRGVHIMV ATPGRLMDM LEKKIVKLS VCRYLCMDE ADRMIDLGF EEDVRTIFS YFNGQRQTL LFSATMPKK IQNFARSAL VKPVTINVG RAGAASMNV IQEVEYVKQ EAKVVYLLE CLQKTPPPV LIFAEKKQD VDAIHEYLL LKGVEAVAI HGGKDQEER SRSVEAFRK HEKDVLVAT DVASKGLDF PDIQHVINY DMPDDVENY VHRIGRTGR SGKQGLATT FINKSNDES VLLDLKHLL MEAKQKVPA FLSELCSES EKYLDLGDE RGCSYCGGL GHRITDCPK LEALQNKQA SNIGRRDYL ANTAADY
- >TC016289 RA
atgggtgat aatgatcct ccagtaaag agatacaga cgtgacgag aaggatcaa gaaaaatca gattcggac gataattac gtcccatac gtgccagtc aaagaacgc aagaagcaa aagcttttg aaactcgga cgattaggc cagctaaaa gagtctgaa ttgaacaaa ccaaaatca tctagcgag aacgaaaca aacgatgac gaagaagag gatgaagta tgggggcgc aagaacaac atttcactt ttggaccaa catacagaa cttaaaaaa ttggcagaa gccaaaaag gtaagcgcc gtggaacgc caattaaaa aaagaagag gaaattctg gaaatagta gcggaaaag aaagccttg atgggcgtt tctgagttg gccaaaggc atacaatac agcgacccg attaaaacc agttggcga ccccctcgt tacgtgctt gcaatgccg gaaaatcgc catgaaaaa atccgaaac gaactccgg attctagtc gaaggtgaa gacattcct ccaccactt aaaaccttt cgcgaaatg aaactacac gaagggatc ataagcgga ctgaaagaa aagaaaatt aagaaacca acaccgatt cagattcaa ggcattcca acagtttta tcaggtcgg gatatgatt gggatagca ttcacgggt tcaggcaaa acactagtt tttgttttg cctctcatc atgttttgt ttggaacag gaagttaaa ttgcccttt attaaaaat gaagggccc tatggactt ataatttgc ccttcgcga gagttggca aagcaaact ttcgatatc atacaacat ttttgtaac agtctgagg aagcatgga atgcccgaa attaggacg tctttagcc attggtgga gtgcctgtt tcagaagct gttgatgtt atacagaga ggtgtgcat attatggtc gccactcca ggacgatta atggacatg cttgagaaa aaaatcgta aaactatcg gtttgtcga tatttgtgt atggatgaa gcggatcgt atgattgac ttgggtttc gaagaggac gttagaaca attttttca tattttaat ggccaaagg cagactttg cttttctca gctactatg cccaagaaa atacaaaat tttgcacgg tcagcctta gtcaaacct gtaacgatc aacgtagga cgggctgga gcagcctca atgaatgtt atccaggag gtggaatac gttaaacaa gaagcaaaa gtggtttac ttgctagaa tgtttgcaa aaaacaccg ccacccgtt ttaattttc gcggagaaa aaacaagac gtggatgcc attcatgaa taccttctt ttgaaagga gttgaagct gttgcaatt catggtggc aaagaccaa gaagaacgt tcgagatcc gttgaagca ttcaggaaa cacgaaaaa gatgttctt gtagctaca gatgtggcc tctaaagga ttagatttt cctgatatc caacatgtt ataaattac gatatgccc gacgatgtg gaaaattat gttcaccga attgggaga actggcaga tccggcaaa caaggccta gctaccaca ttcataaac aaatccaac gacgaatct gtcctcctc gacctgaaa cacctcctt atggaagcc aaacaaaag gtccccgct ttcttgagc gaattatgc tcagaaagt gagaaatat ctagacttg ggagatgag agaggttgt agctattgc ggaggtttg ggtcacaga atcaccgac tgtccgaaa ttggaagca cttcaaaac aaacaagcg tcaaacatt ggcaggagg gattatttg gccaatact gcagcagat tattag
- >TC016289 RA
ggcgagaaa taatacagt ttgacaagg gggcgacct tgagcataa cctcaaaaa atacgtttt gtttatgtg tgaatttct tgaaaatgg gtgataatg atcctccag taaagagat acagacgtg acgagaagg atcaagaaa aatcagatt cggacgata attacgtcc catacgtgc cagtcaaag aacgcaaga agcaaaagc ttttgaaac tcggacgat taggccagc taaaagagt ctgaattga acaaaccaa aatcatcta gcgagaacg aaacaaacg atgacgaag aagaggatg aagtatggg ggcgcaaga acaacattt cacttttgg accaacata cagaactta aaaaattgg cagaagcca aaaaggtaa gcgccgtgg aacgccaat taaaaaaag aagaggaaa ttctggaaa tagtagcgg aaaagaaag ccttgatgg gcgtttctg agttggcca aaggcatac aatacagcg acccgatta aaaccagtt ggcgacccc ctcgttacg tgcttgcaa tgccggaaa atcgccatg aaaaaatcc gaaacgaac tccggattc tagtcgaag gtgaagaca ttcctccac cacttaaaa cctttcgcg aaatgaaac tacacgaag ggatcataa gcggactga aagaaaaga aaattaaga aaccaacac cgattcaga ttcaaggca ttccaacag ttttatcag gtcgggata tgattggga tagcattca cgggttcag gcaaaacac tagtttttg ttttgcctc tcatcatgt tttgtttgg aacaggaag ttaaattgc cctttatta aaaatgaag ggccctatg gacttataa tttgccctt cgcgagagt tggcaaagc aaactttcg atatcatac aacattttt gtaacagtc tgaggaagc atggaatgc ccgaaatta ggacgtctt tagccattg gtggagtgc ctgtttcag aagctgttg atgttatac agagaggtg tgcatatta tggtcgcca ctccaggac gattaatgg acatgcttg agaaaaaaa tcgtaaaac tatcggttt gtcgatatt tgtgtatgg atgaagcgg atcgtatga ttgacttgg gtttcgaag aggacgtta gaacaattt tttcatatt ttaatggcc aaaggcaga ctttgcttt tctcagcta ctatgccca agaaaatac aaaattttg cacggtcag ccttagtca aacctgtaa cgatcaacg taggacggg ctggagcag cctcaatga atgttatcc aggaggtgg aatacgtta aacaagaag caaaagtgg tttacttgc tagaatgtt tgcaaaaaa caccgccac ccgttttaa ttttcgcgg agaaaaaac aagacgtgg atgccattc atgaatacc ttcttttga aaggagttg aagctgttg caattcatg gtggcaaag accaagaag aacgttcga gatccgttg aagcattca ggaaacacg aaaaagatg ttcttgtag ctacagatg tggcctcta aaggattag attttcctg atatccaac atgttataa attacgata tgcccgacg atgtggaaa attatgttc accgaattg ggagaactg gcagatccg gcaaacaag gcctagcta ccacattca taaacaaat ccaacgacg aatctgtcc tcctcgacc tgaaacacc tccttatgg aagccaaac aaaaggtcc ccgctttct tgagcgaat tatgctcag aaagtgaga aatatctag acttgggag atgagagag gttgtagct attgcggag gtttgggtc acagaatca ccgactgtc cgaaattgg aagcacttc aaaacaaac aagcgtcaa acattggca ggagggatt atttggcca atactgcag cagattatt agattttaa taacatttt attaatatt cccaaatct tacaatata aaatattac atttacgga aaatattat gatttatat tatgtatta caaattgaa ttcattctt aaaaaaatc gtaagcttt atttcgtta agcatgtgc gccaataac aacttctgt agcatgttt ctataacga gtgacaaaa aaatctaca ttgactaga agtattaac cacgtagtt tcaaaacat ttaagcaca gctttctta atcagagaa ggaagtgtt tcttcactc tgcattttt tacaaaaaa aactcttgt cttgtttga ttagacgga tccacatca tttccccaa acactgcgc aactgagaa agattttga tcaaaatta aaaacggtc aatgatgtg aaaataatg tctctaacg ctaggggca cacttaaat tgtaatgtc ggtggaaca aattcagga taaattata gcttattag actagatag ctacagccg tacgttaaa aaaaaatgc ttttgagta gctttggaa aaaattaaa atatttaat agcaaacgc tgatttttg tgtgacaat tagtttttg agatatagt aaaaagtac ttataataa acagactca ctgtcgcca acattcggc aagacatat attttttaa atactgtta atattacaa tatatatca ctatttcat ataattcat acgaaaatg tgccaatga gatggtttc gaagttttt aaattgaga aactgttaa tttttgtgg agcaatggt atggatatt tctgtttat taataacta ttttattca tagggtgtt ttagaaaca aaattaata gtaatgtaa taataatga ataataata ataaataac aaaactatt a
