Status
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Gene information
General information
TC016289 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10244782..10252460
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016289
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06476

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016289 RA
      MGDNDPPVK RYRRDEKDQ EKSDSDDNY VPYVPVKER 
      KKQKLLKLG RLGQLKESE LNKPKSSSE NETNDDEEE 
      DEVWGRKNN ISLLDQHTE LKKLAEAKK VSAVERQLK 
      KEEEILEIV AEKKALMGV SELAKGIQY SDPIKTSWR 
      PPRYVLAMP ENRHEKIRN ELRILVEGE DIPPPLKTF 
      REMKLHEGI ISGLKEKKI KKPTPIQIQ GIPTVLSGR 
      DMIGIAFTG SGKTLVFVL PLIMFCLEQ EVKLPFIKN 
      EGPYGLIIC PSRELAKQT FDIIQHFCN SLRKHGMPE 
      IRTSLAIGG VPVSEAVDV IQRGVHIMV ATPGRLMDM 
      LEKKIVKLS VCRYLCMDE ADRMIDLGF EEDVRTIFS 
      YFNGQRQTL LFSATMPKK IQNFARSAL VKPVTINVG 
      RAGAASMNV IQEVEYVKQ EAKVVYLLE CLQKTPPPV 
      LIFAEKKQD VDAIHEYLL LKGVEAVAI HGGKDQEER 
      SRSVEAFRK HEKDVLVAT DVASKGLDF PDIQHVINY 
      DMPDDVENY VHRIGRTGR SGKQGLATT FINKSNDES 
      VLLDLKHLL MEAKQKVPA FLSELCSES EKYLDLGDE 
      RGCSYCGGL GHRITDCPK LEALQNKQA SNIGRRDYL 
      ANTAADY
    CDS sequence
    • >TC016289 RA
      atgggtgat aatgatcct ccagtaaag agatacaga 
      cgtgacgag aaggatcaa gaaaaatca gattcggac 
      gataattac gtcccatac gtgccagtc aaagaacgc 
      aagaagcaa aagcttttg aaactcgga cgattaggc 
      cagctaaaa gagtctgaa ttgaacaaa ccaaaatca 
      tctagcgag aacgaaaca aacgatgac gaagaagag 
      gatgaagta tgggggcgc aagaacaac atttcactt 
      ttggaccaa catacagaa cttaaaaaa ttggcagaa 
      gccaaaaag gtaagcgcc gtggaacgc caattaaaa 
      aaagaagag gaaattctg gaaatagta gcggaaaag 
      aaagccttg atgggcgtt tctgagttg gccaaaggc 
      atacaatac agcgacccg attaaaacc agttggcga 
      ccccctcgt tacgtgctt gcaatgccg gaaaatcgc 
      catgaaaaa atccgaaac gaactccgg attctagtc 
      gaaggtgaa gacattcct ccaccactt aaaaccttt 
      cgcgaaatg aaactacac gaagggatc ataagcgga 
      ctgaaagaa aagaaaatt aagaaacca acaccgatt 
      cagattcaa ggcattcca acagtttta tcaggtcgg 
      gatatgatt gggatagca ttcacgggt tcaggcaaa 
      acactagtt tttgttttg cctctcatc atgttttgt 
      ttggaacag gaagttaaa ttgcccttt attaaaaat 
      gaagggccc tatggactt ataatttgc ccttcgcga 
      gagttggca aagcaaact ttcgatatc atacaacat 
      ttttgtaac agtctgagg aagcatgga atgcccgaa 
      attaggacg tctttagcc attggtgga gtgcctgtt 
      tcagaagct gttgatgtt atacagaga ggtgtgcat 
      attatggtc gccactcca ggacgatta atggacatg 
      cttgagaaa aaaatcgta aaactatcg gtttgtcga 
      tatttgtgt atggatgaa gcggatcgt atgattgac 
      ttgggtttc gaagaggac gttagaaca attttttca 
      tattttaat ggccaaagg cagactttg cttttctca 
      gctactatg cccaagaaa atacaaaat tttgcacgg 
      tcagcctta gtcaaacct gtaacgatc aacgtagga 
      cgggctgga gcagcctca atgaatgtt atccaggag 
      gtggaatac gttaaacaa gaagcaaaa gtggtttac 
      ttgctagaa tgtttgcaa aaaacaccg ccacccgtt 
      ttaattttc gcggagaaa aaacaagac gtggatgcc 
      attcatgaa taccttctt ttgaaagga gttgaagct 
      gttgcaatt catggtggc aaagaccaa gaagaacgt 
      tcgagatcc gttgaagca ttcaggaaa cacgaaaaa 
      gatgttctt gtagctaca gatgtggcc tctaaagga 
      ttagatttt cctgatatc caacatgtt ataaattac 
      gatatgccc gacgatgtg gaaaattat gttcaccga 
      attgggaga actggcaga tccggcaaa caaggccta 
      gctaccaca ttcataaac aaatccaac gacgaatct 
      gtcctcctc gacctgaaa cacctcctt atggaagcc 
      aaacaaaag gtccccgct ttcttgagc gaattatgc 
      tcagaaagt gagaaatat ctagacttg ggagatgag 
      agaggttgt agctattgc ggaggtttg ggtcacaga 
      atcaccgac tgtccgaaa ttggaagca cttcaaaac 
      aaacaagcg tcaaacatt ggcaggagg gattatttg 
      gccaatact gcagcagat tattag
    mRNA sequence
    • >TC016289 RA
      ggcgagaaa taatacagt ttgacaagg gggcgacct 
      tgagcataa cctcaaaaa atacgtttt gtttatgtg 
      tgaatttct tgaaaatgg gtgataatg atcctccag 
      taaagagat acagacgtg acgagaagg atcaagaaa 
      aatcagatt cggacgata attacgtcc catacgtgc 
      cagtcaaag aacgcaaga agcaaaagc ttttgaaac 
      tcggacgat taggccagc taaaagagt ctgaattga 
      acaaaccaa aatcatcta gcgagaacg aaacaaacg 
      atgacgaag aagaggatg aagtatggg ggcgcaaga 
      acaacattt cacttttgg accaacata cagaactta 
      aaaaattgg cagaagcca aaaaggtaa gcgccgtgg 
      aacgccaat taaaaaaag aagaggaaa ttctggaaa 
      tagtagcgg aaaagaaag ccttgatgg gcgtttctg 
      agttggcca aaggcatac aatacagcg acccgatta 
      aaaccagtt ggcgacccc ctcgttacg tgcttgcaa 
      tgccggaaa atcgccatg aaaaaatcc gaaacgaac 
      tccggattc tagtcgaag gtgaagaca ttcctccac 
      cacttaaaa cctttcgcg aaatgaaac tacacgaag 
      ggatcataa gcggactga aagaaaaga aaattaaga 
      aaccaacac cgattcaga ttcaaggca ttccaacag 
      ttttatcag gtcgggata tgattggga tagcattca 
      cgggttcag gcaaaacac tagtttttg ttttgcctc 
      tcatcatgt tttgtttgg aacaggaag ttaaattgc 
      cctttatta aaaatgaag ggccctatg gacttataa 
      tttgccctt cgcgagagt tggcaaagc aaactttcg 
      atatcatac aacattttt gtaacagtc tgaggaagc 
      atggaatgc ccgaaatta ggacgtctt tagccattg 
      gtggagtgc ctgtttcag aagctgttg atgttatac 
      agagaggtg tgcatatta tggtcgcca ctccaggac 
      gattaatgg acatgcttg agaaaaaaa tcgtaaaac 
      tatcggttt gtcgatatt tgtgtatgg atgaagcgg 
      atcgtatga ttgacttgg gtttcgaag aggacgtta 
      gaacaattt tttcatatt ttaatggcc aaaggcaga 
      ctttgcttt tctcagcta ctatgccca agaaaatac 
      aaaattttg cacggtcag ccttagtca aacctgtaa 
      cgatcaacg taggacggg ctggagcag cctcaatga 
      atgttatcc aggaggtgg aatacgtta aacaagaag 
      caaaagtgg tttacttgc tagaatgtt tgcaaaaaa 
      caccgccac ccgttttaa ttttcgcgg agaaaaaac 
      aagacgtgg atgccattc atgaatacc ttcttttga 
      aaggagttg aagctgttg caattcatg gtggcaaag 
      accaagaag aacgttcga gatccgttg aagcattca 
      ggaaacacg aaaaagatg ttcttgtag ctacagatg 
      tggcctcta aaggattag attttcctg atatccaac 
      atgttataa attacgata tgcccgacg atgtggaaa 
      attatgttc accgaattg ggagaactg gcagatccg 
      gcaaacaag gcctagcta ccacattca taaacaaat 
      ccaacgacg aatctgtcc tcctcgacc tgaaacacc 
      tccttatgg aagccaaac aaaaggtcc ccgctttct 
      tgagcgaat tatgctcag aaagtgaga aatatctag 
      acttgggag atgagagag gttgtagct attgcggag 
      gtttgggtc acagaatca ccgactgtc cgaaattgg 
      aagcacttc aaaacaaac aagcgtcaa acattggca 
      ggagggatt atttggcca atactgcag cagattatt 
      agattttaa taacatttt attaatatt cccaaatct 
      tacaatata aaatattac atttacgga aaatattat 
      gatttatat tatgtatta caaattgaa ttcattctt 
      aaaaaaatc gtaagcttt atttcgtta agcatgtgc 
      gccaataac aacttctgt agcatgttt ctataacga 
      gtgacaaaa aaatctaca ttgactaga agtattaac 
      cacgtagtt tcaaaacat ttaagcaca gctttctta 
      atcagagaa ggaagtgtt tcttcactc tgcattttt 
      tacaaaaaa aactcttgt cttgtttga ttagacgga 
      tccacatca tttccccaa acactgcgc aactgagaa 
      agattttga tcaaaatta aaaacggtc aatgatgtg 
      aaaataatg tctctaacg ctaggggca cacttaaat 
      tgtaatgtc ggtggaaca aattcagga taaattata 
      gcttattag actagatag ctacagccg tacgttaaa 
      aaaaaatgc ttttgagta gctttggaa aaaattaaa 
      atatttaat agcaaacgc tgatttttg tgtgacaat 
      tagtttttg agatatagt aaaaagtac ttataataa 
      acagactca ctgtcgcca acattcggc aagacatat 
      attttttaa atactgtta atattacaa tatatatca 
      ctatttcat ataattcat acgaaaatg tgccaatga 
      gatggtttc gaagttttt aaattgaga aactgttaa 
      tttttgtgg agcaatggt atggatatt tctgtttat 
      taataacta ttttattca tagggtgtt ttagaaaca 
      aaattaata gtaatgtaa taataatga ataataata 
      ataaataac aaaactatt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY