Status
Please wait ...
Gene information
General information
TC032110
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000643
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8589696..8595495
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032110
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06474

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032110 RA
      MDVINFCRF GYVLILASF FLMIWISLT GAREIDLVR 
      YPSCYFNSL CACSKSGPD LGIVRCQNI HLPRIPETV 
      NISKVFMLH LENNNLKVL EPYFLQSTG LYKIVISKN 
      PLSYIPDET FLGLERSLW ELDLSHNQL TRVPNRAIR 
      YLRKLRYLN LRGNNIARI SPENWRGLE NSLTTLILT 
      DNYITHLPT DSFSGLPMV ETIDLRGNN LKEIDPSVF 
      RDGMGRLSN LILADNQLS AIPYQALSF LKSLRELDL 
      SHNKINTMQ PAADVGVQN VNYNFLFNL DSLRLDYNQ 
      ITMLRAASF QYFNVLNRT YLDGNPLSL VEENAFRQA 
      KIKELYLRD CGLTEISPL AFSGLENFL EILDLSGNH 
      ISSLSDDVF QRLELLKTL SLRENTLKQ LNAVQSLNG 
      ARFSLYHLD LSGNENAPV SLQELKKLR NLRILSLSR 
      LSQPSLSAD DFRDFGIDL EELHVTYGG LQTIKNNAF 
      KYVHALKKL QLSDNVISS IESNAFVDI GDSLLTLKL 
      THALSSSVQ NFPSDAIKI LNRLEELDL SNNRLRNVP 
      DNSFHFLRS LKKVHLQDN TIEMIHRGT FQGDIHRDL 
      TEVYFSFNS VRNVQQHTF ADLIQLEQI HLDDNRIES 
      LERRAFMNL KSLKRLNLK GNKIATIAY ETFQNLPEL 
      EDLDLAYNS ISSLDFNIF DQVGSLGMF HVNMSHNKL 
      INLVVAPSV PFEQDTGLG GLQNIKVLD LSFNNITSV 
      AKQFFRPVE LSLMQLYLG HNKLLNATK DLFGNMPHL 
      QVLDLSHNS LYELDFDTF RNTKKLQWL DTSHNRISE 
      IPNDLFRFL GNLRIVDFS HNRLRSLPD NLFRETGLE 
      RLDVSHNLL GKLPLTSLS LASAQTLSE LDLSWNSIS 
      SLSHGGQLA RFKCLSWLD LSYNRLGQI DAGTFKGIP 
      RLASLNLGH NSQLTLEIN GLSFQGLEY TLLHLNLDN 
      VSLSQVPAL STPNLLSLS LAFNSLPTV ALEVAGNIS 
      SLRYLNLDY NDLSAVPIV THSLTELRH LSLEGNPIT 
      TLSNTSLLG AANQLEELN LKNIDLTVL ESGAFCKMQ 
      PLRTLKIGV YRNIKNFNI PSILQFNDG LKNLEIHVT 
      KDTDTLDNE MRGAWPLKL QNLTLSGKG LKKITGKMF 
      QGVRSPTFH LTVRNTSII KIPFDVFRN MLPVRNVSV 
      DVRDNILLK NLQNPSTGS KPGKPRSAF LTDLKLMGG 
      KWSCDCDLG WVEVWERKR RQYLCPSVA SSLDFEEYN 
      CRHTDDGLR NTLCANKNN RSLVEVLKS DIECGWSKA 
      SMEKLMIWL VVLCQIVVN FV
    CDS sequence
    • >TC032110 RA
      atggacgtg attaacttt tgccgcttt ggctacgtt 
      ctaatcctt gcaagtttt ttcctaatg atttggatt 
      tcgttaact ggggcgcgt gaaatagat ttagtgcgt 
      tatccttcc tgttatttt aattcactg tgtgcatgt 
      tcaaaaagt ggccccgat ttaggcata gttaggtgt 
      caaaacatc cacttgccc cgaattcca gaaactgta 
      aatatctcc aaagtgttt atgctacac ctggaaaat 
      aacaatttg aaagtcctt gagccgtat tttttgcaa 
      agtacaggg ttgtataaa attgtgata agcaagaat 
      cctctatca tacattcct gatgaaaca tttctgggg 
      cttgaacgg tccttgtgg gaattagac ctgagccac 
      aatcaatta acacgtgtt ccaaaccgc gcaatacga 
      tatttacga aaactgaga tatttaaac ttgagaggg 
      aacaatatt gcaaggata tcgcccgaa aattggaga 
      ggacttgag aactctctc acaactttg attctcact 
      gataattac attacccat ttgccgacc gactcattc 
      tctggttta ccaatggtt gagacaatc gatttgaga 
      ggaaacaac ttgaaagaa atcgatcct tcggttttc 
      agagatggc atgggccga ttatctaat ctgatccta 
      gccgataac caactatcc gcaattccg taccaagcg 
      ctctcattt ttaaaatcc ttgcgggaa ttagatttg 
      agtcacaat aaaattaat acgatgcaa ccggcagca 
      gacgtggga gttcaaaac gtgaattac aatttcctc 
      ttcaatttg gattcgtta agacttgac tataatcaa 
      ataacaatg ctgagggcc gcctccttc cagtacttc 
      aacgtcttg aacaggacg tacttggac ggcaatccc 
      ctgagcctt gttgaggaa aatgcgttc agacaagcg 
      aaaatcaaa gaactgtac ttgagagat tgcggtttg 
      acggaaata tctccgctg gccttttcc ggcctggag 
      aattttctc gaaattttg gacctctcg ggtaaccac 
      atttcgtcg ctttctgac gacgttttc caacgactg 
      gagctgctg aaaacgctg tcgctgcgc gaaaacact 
      ttgaaacaa ttaaacgcc gtgcagtca ctcaatggg 
      gcccgcttt tccctttac catctcgat ttgagtgga 
      aatgaaaat gccccagtc tcgctccaa gaactgaaa 
      aagctccgc aatttgaga atcttatca ctgtctcgc 
      ttatctcaa ccgagtctc tctgcggac gatttccgc 
      gattttgga atcgatttg gaagaactg cacgttacc 
      tatgggggg cttcaaaca atcaaaaat aacgcgttt 
      aaatacgtc catgctttg aaaaaatta caactttct 
      gataatgtc atttcaagc atcgaaagc aacgctttt 
      gttgatatt ggtgattcg ttactgacg ttgaaacta 
      acccatgct ctgtcctca tcggtccaa aattttcca 
      agtgacgct ataaaaatt ttaaatcga ctggaagag 
      ctcgacttg agcaacaac cggctcagg aacgtcccg 
      gacaattcc ttccacttc ctgagaagt ttgaaaaaa 
      gtgcacctg caggacaac accattgag atgatccac 
      agagggacg ttccagggg gacattcac cgcgatttg 
      accgaagtc tatttctcg tttaacagc gttcggaac 
      gtccaacaa cacacattt gccgatcta atccaactc 
      gagcaaatt cacttggac gacaaccga atcgaaagt 
      ctagaacga cgcgctttc atgaacctg aaaagcctg 
      aagcgactg aacttgaaa ggcaacaaa atcgccaca 
      atcgcgtac gaaactttc cagaatttg cccgaactt 
      gaggacttg gatttggcc tacaacagc atcagtagt 
      cttgatttt aacattttc gaccaagtg gggtcgctg 
      ggaatgttt catgttaac atgagccac aataaactg 
      atcaatttg gttgttgct ccttcggtg ccgtttgaa 
      caagataca gggcttggc ggcttgcag aacatcaaa 
      gtgttggac ttgtcgttc aacaacatc acgtcggtc 
      gccaaacag ttcttcagg ccggttgaa ttgtcccta 
      atgcagctt tacctcggg cataacaaa cttttaaac 
      gccactaag gaccttttc gggaacatg ccccacttg 
      caagtgctc gatttgtca cacaattct ttgtacgaa 
      ctggacttt gacactttc aggaatacg aaaaaactt 
      cagtggctt gatacgtcc cataatcgc atttccgag 
      atcccaaac gacttgttt cggtttttg ggcaacttg 
      aggattgtt gatttttct cataataga ctgcgatcc 
      ctacctgac aatttattc agagagacg ggccttgaa 
      cgcttggat gtgtcacac aatttactg ggcaaatta 
      ccacttaca agtctttcg cttgcgtcg gctcaaact 
      ttaagcgag ctggacttg tcctggaac agcatttcg 
      tccttatct cacggtgga caattagcg cggtttaaa 
      tgcctctcc tggctggac ttgtcttat aatcggttg 
      ggtcagatc gatgctggg acttttaag ggcatcccg 
      aggctggca agcctcaat ttgggtcac aacagccaa 
      ctcacctta gaaataaac ggtttgagc ttccaggga 
      ttggagtac accctcctc catttgaat ctggacaac 
      gtttcgttg tcccaggtg ccagcacta tcaacgcct 
      aatctactt tcactatca cttgctttt aattcttta 
      ccaactgtt gcacttgaa gtagcaggg aacatatca 
      tcgctgcgg tatttaaat ttggattat aatgacttg 
      agtgctgtt ccaatagtg acacactct ttgaccgaa 
      ttgaggcat ttgtcactc gaggggaat cccatcacg 
      actttgagc aacacgagt ttgctggga gccgcgaat 
      cagttggaa gaactcaac ttgaaaaac atcgacttg 
      acggttttg gaatcgggc gctttttgc aaaatgcag 
      cctttgagg acactcaag attggcgtt taccgcaat 
      attaaaaac tttaacata ccgtcaatt ttgcaattt 
      aatgatgga ctcaagaat ttggaaatt cacgtgact 
      aaagacacc gatacttta gacaatgag atgcgcggg 
      gcttggccc ttgaaactg caaaacctg actttgagc 
      ggaaaaggc ttgaagaaa atcacggga aaaatgttt 
      cagggggtt cgaagtccg acttttcat ctaactgtc 
      cgtaacacc agcattatt aaaatccct tttgacgtt 
      ttccgcaac atgttgcca gtgagaaac gtgagtgtc 
      gacgttcgg gataatatc cttctgaag aatttgcaa 
      aatccgtca actgggtcc aaacctggc aagcctagg 
      tcggcgttt ttgaccgat ttgaaactg atgggcgga 
      aaatggtct tgcgattgt gatttgggg tgggttgaa 
      gtttgggag aggaaacgg cggcaatat ttatgcccc 
      agtgttgct tcgagtctc gattttgag gaatataat 
      tgtaggcac actgatgat ggtcttagg aacacgttg 
      tgtgcgaat aaaaataac cggagtttg gttgaagtt 
      cttaagagt gatattgag tgtggttgg agtaaagct 
      tcgatggag aaattgatg atttggctc gttgtactt 
      tgccaaatt gttgtaaac tttgtttag
    mRNA sequence
    • >TC032110 RA
      tcattgttg tagtttatt gcaaactag tcgtagtta 
      gacgatgtg tgtgttttc cagtgtatt tcttaatta 
      gttaaaatg gacgtgatt aacttttgc cgctttggc 
      tacgttcta atccttgca agttttttc ctaatgatt 
      tggatttcg ttaactggg gcgcgtgaa atagattta 
      gtgcgttat ccttcctgt tattttaat tcactgtgt 
      gcatgttca aaaagtggc cccgattta ggcatagtt 
      aggtgtcaa aacatccac ttgccccga attccagaa 
      actgtaaat atctccaaa gtgtttatg ctacacctg 
      gaaaataac aatttgaaa gtccttgag ccgtatttt 
      ttgcaaagt acagggttg tataaaatt gtgataagc 
      aagaatcct ctatcatac attcctgat gaaacattt 
      ctggggctt gaacggtcc ttgtgggaa ttagacctg 
      agccacaat caattaaca cgtgttcca aaccgcgca 
      atacgatat ttacgaaaa ctgagatat ttaaacttg 
      agagggaac aatattgca aggatatcg cccgaaaat 
      tggagagga cttgagaac tctctcaca actttgatt 
      ctcactgat aattacatt acccatttg ccgaccgac 
      tcattctct ggtttacca atggttgag acaatcgat 
      ttgagagga aacaacttg aaagaaatc gatccttcg 
      gttttcaga gatggcatg ggccgatta tctaatctg 
      atcctagcc gataaccaa ctatccgca attccgtac 
      caagcgctc tcattttta aaatccttg cgggaatta 
      gatttgagt cacaataaa attaatacg atgcaaccg 
      gcagcagac gtgggagtt caaaacgtg aattacaat 
      ttcctcttc aatttggat tcgttaaga cttgactat 
      aatcaaata acaatgctg agggccgcc tccttccag 
      tacttcaac gtcttgaac aggacgtac ttggacggc 
      aatcccctg agccttgtt gaggaaaat gcgttcaga 
      caagcgaaa atcaaagaa ctgtacttg agagattgc 
      ggtttgacg gaaatatct ccgctggcc ttttccggc 
      ctggagaat tttctcgaa attttggac ctctcgggt 
      aaccacatt tcgtcgctt tctgacgac gttttccaa 
      cgactggag ctgctgaaa acgctgtcg ctgcgcgaa 
      aacactttg aaacaatta aacgccgtg cagtcactc 
      aatggggcc cgcttttcc ctttaccat ctcgatttg 
      agtggaaat gaaaatgcc ccagtctcg ctccaagaa 
      ctgaaaaag ctccgcaat ttgagaatc ttatcactg 
      tctcgctta tctcaaccg agtctctct gcggacgat 
      ttccgcgat tttggaatc gatttggaa gaactgcac 
      gttacctat ggggggctt caaacaatc aaaaataac 
      gcgtttaaa tacgtccat gctttgaaa aaattacaa 
      ctttctgat aatgtcatt tcaagcatc gaaagcaac 
      gcttttgtt gatattggt gattcgtta ctgacgttg 
      aaactaacc catgctctg tcctcatcg gtccaaaat 
      tttccaagt gacgctata aaaatttta aatcgactg 
      gaagagctc gacttgagc aacaaccgg ctcaggaac 
      gtcccggac aattccttc cacttcctg agaagtttg 
      aaaaaagtg cacctgcag gacaacacc attgagatg 
      atccacaga gggacgttc cagggggac attcaccgc 
      gatttgacc gaagtctat ttctcgttt aacagcgtt 
      cggaacgtc caacaacac acatttgcc gatctaatc 
      caactcgag caaattcac ttggacgac aaccgaatc 
      gaaagtcta gaacgacgc gctttcatg aacctgaaa 
      agcctgaag cgactgaac ttgaaaggc aacaaaatc 
      gccacaatc gcgtacgaa actttccag aatttgccc 
      gaacttgag gacttggat ttggcctac aacagcatc 
      agtagtctt gattttaac attttcgac caagtgggg 
      tcgctggga atgtttcat gttaacatg agccacaat 
      aaactgatc aatttggtt gttgctcct tcggtgccg 
      tttgaacaa gatacaggg cttggcggc ttgcagaac 
      atcaaagtg ttggacttg tcgttcaac aacatcacg 
      tcggtcgcc aaacagttc ttcaggccg gttgaattg 
      tccctaatg cagctttac ctcgggcat aacaaactt 
      ttaaacgcc actaaggac cttttcggg aacatgccc 
      cacttgcaa gtgctcgat ttgtcacac aattctttg 
      tacgaactg gactttgac actttcagg aatacgaaa 
      aaacttcag tggcttgat acgtcccat aatcgcatt 
      tccgagatc ccaaacgac ttgtttcgg tttttgggc 
      aacttgagg attgttgat ttttctcat aatagactg 
      cgatcccta cctgacaat ttattcaga gagacgggc 
      cttgaacgc ttggatgtg tcacacaat ttactgggc 
      aaattacca cttacaagt ctttcgctt gcgtcggct 
      caaacttta agcgagctg gacttgtcc tggaacagc 
      atttcgtcc ttatctcac ggtggacaa ttagcgcgg 
      tttaaatgc ctctcctgg ctggacttg tcttataat 
      cggttgggt cagatcgat gctgggact tttaagggc 
      atcccgagg ctggcaagc ctcaatttg ggtcacaac 
      agccaactc accttagaa ataaacggt ttgagcttc 
      cagggattg gagtacacc ctcctccat ttgaatctg 
      gacaacgtt tcgttgtcc caggtgcca gcactatca 
      acgcctaat ctactttca ctatcactt gcttttaat 
      tctttacca actgttgca cttgaagta gcagggaac 
      atatcatcg ctgcggtat ttaaatttg gattataat 
      gacttgagt gctgttcca atagtgaca cactctttg 
      accgaattg aggcatttg tcactcgag gggaatccc 
      atcacgact ttgagcaac acgagtttg ctgggagcc 
      gcgaatcag ttggaagaa ctcaacttg aaaaacatc 
      gacttgacg gttttggaa tcgggcgct ttttgcaaa 
      atgcagcct ttgaggaca ctcaagatt ggcgtttac 
      cgcaatatt aaaaacttt aacataccg tcaattttg 
      caatttaat gatggactc aagaatttg gaaattcac 
      gtgactaaa gacaccgat actttagac aatgagatg 
      cgcggggct tggcccttg aaactgcaa aacctgact 
      ttgagcgga aaaggcttg aagaaaatc acgggaaaa 
      atgtttcag ggggttcga agtccgact tttcatcta 
      actgtccgt aacaccagc attattaaa atccctttt 
      gacgttttc cgcaacatg ttgccagtg agaaacgtg 
      agtgtcgac gttcgggat aatatcctt ctgaagaat 
      ttgcaaaat ccgtcaact gggtccaaa cctggcaag 
      cctaggtcg gcgtttttg accgatttg aaactgatg 
      ggcggaaaa tggtcttgc gattgtgat ttggggtgg 
      gttgaagtt tgggagagg aaacggcgg caatattta 
      tgccccagt gttgcttcg agtctcgat tttgaggaa 
      tataattgt aggcacact gatgatggt cttaggaac 
      acgttgtgt gcgaataaa aataaccgg agtttggtt 
      gaagttctt aagagtgat attgagtgt ggttggagt 
      aaagcttcg atggagaaa ttgatgatt tggctcgtt 
      gtactttgc caaattgtt gtaaacttt gtttaggat 
      taatgcctt atgtgttcg ctgttgcac atcctcgac 
      ttgaaacga ataaactac tttagacgt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY