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Gene information
General information
TC012508 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9624906..9631499
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012508
    iBeetle screen iB_06472

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012508 RA
      MAASDSAGD DSLYPIAVL IDELKNEDV QLRLNSIKK 
      LSTIALALG VERTRSELI PFLTETIYD EDEVLLALA 
      EQLGSFITL VGGPEYAYC LLPPLESLA TVEETVVRD 
      KAVESLRAV AQQHSPADL ENHFVPLVQ RLSAGDWFT 
      SRTSACGLF SVCYPRVSA QMKADLRSH FRALCQDDT 
      PMVRRAAAT KLGELAQVV ELEYLKTDL IPMFLSLTQ 
      DEQDSVRLL AVEACVSFA SLFQQEDIE MFVMPTVRN 
      CTGDTSWRV RYMVADKFT DLQKAVGPE ITRTDLVPA 
      FQNLLKDTE AEVRAAAAN KVKDFCQNL DKAHQESII 
      MNNILPCVK ELVADPNQH VKSALASVI MGLSPILGR 
      HNTIEHLLP LFLTQLKDE CPEVRLNII SNLDCVNEV 
      IGIQQLSQS LLPAIVELA EDSKWRVRS AIIEYMPLL 
      AGQLGREFF DEKLNALCM TWLMDHVFA IREAATLNL 
      RKLVDQFGA EWAETTIIP KVLAMSRDQ NYLYRMTCL 
      FCINVLAEA CGSDITTRL LLPTVLSMA NDKVANVRF 
      NVAKTLQKI APQLDQAVI QPQVKPVLD KLTADSDID 
      VKYSASEAI AGIAALG
    CDS sequence
    • >TC012508 RA
      atggcagcc agcgattca gcaggcgac gattccttg 
      taccccatc gccgtccta atcgacgag ctcaaaaac 
      gaggatgtc cagctccga ctcaactcc atcaagaag 
      ctctccacg atcgcattg gccctgggg gtggagcgc 
      acccgctcc gagctcatc cccttcctc acggagacc 
      atctacgac gaggacgag gtgctccta gcccttgcc 
      gagcaactg gggagtttc atcacgtta gttggcggg 
      cctgaatac gcctactgt cttttaccg ccgcttgag 
      agcctggcc actgttgag gaaacagtc gtcagggac 
      aaggcagtg gagtcgttg agggcggtg gcgcagcaa 
      cacagccca gctgattta gaaaaccac tttgtgccc 
      ctagtccag agactgtca gcaggggat tggtttact 
      tcgcggact tcggcatgt gggctattc tccgtttgt 
      tacccccgc gtgtcggct caaatgaag gcggatttg 
      cgctcgcat tttcgggct ttgtgtcag gacgacacg 
      ccgatggtt aggcgagct gccgccacg aaattaggc 
      gaattggca caagtcgtc gagttggag tatttaaaa 
      accgattta attccgatg tttttgagt ctgacccaa 
      gatgaacaa gattcagtt aggcttttg gcagttgag 
      gcttgtgtc agttttgcc tcacttttt caacaagag 
      gatatcgag atgtttgtg atgccgact gttaggaat 
      tgcaccggt gatacatca tggcgtgtg cgctacatg 
      gttgcggac aaattcacc gatttgcaa aaggccgta 
      ggaccagaa atcacccga accgattta gtccccgcc 
      tttcagaac ctcctcaag gacactgaa gccgaagtt 
      cgggccgca gccgccaat aaagtcaaa gatttctgt 
      caaaacttg gacaaagct catcaggag agcatcatt 
      atgaacaat atccttccc tgcgttaaa gaactcgtc 
      gccgatcct aaccaacat gtcaagtcg gccttggct 
      tcggtgata atgggcctg agccctatt ctcggccgt 
      cacaatact atcgaacat ctccttcct ttgtttttg 
      actcaattg aaagacgaa tgtcctgaa gttcgcttg 
      aatatcatc tcgaatcta gattgcgtg aatgaggtc 
      ataggaatc cagcaattg tctcagagt ttattgccg 
      gccatcgtc gaattggcc gaggattca aaatggcgc 
      gtccgttcg gccatcatc gaatacatg ccattgttg 
      gctggccaa ttgggacgc gagtttttc gatgagaaa 
      ttaaacgca ctgtgcatg acttggttg atggatcat 
      gtgttcgct attcgtgaa gcggcaact ttgaatttg 
      aggaagttg gtggatcag tttggggct gaatgggcc 
      gaaaccacg atcattccg aaggttttg gcaatgtcc 
      cgtgaccag aattatttg tataggatg acttgtctg 
      ttttgcatc aatgtgttg gcagaggcc tgcggtagc 
      gatattacg acgcgattg ttgttacca actgttctg 
      agtatggcg aatgataaa gtcgccaat gtaaggttt 
      aacgtcgct aaaacgtta caaaaaatc gcaccgcag 
      ttggaccaa gcggtcatc cagccccaa gttaagcct 
      gttttagac aaattaaca gcggatagc gatatcgat 
      gtcaagtat tcggcgtcc gaagccatc gctgggatt 
      gctgcgtta ggttga
    mRNA sequence
    • >TC012508 RA
      tacgaacgg caactcgtc agaggcact tttagtggc 
      gtttgacaa cgaattttg aaattagtg tgtgtcagt 
      gactgtatt tacaaacac taaagacga cggtatcgg 
      cgatttcaa cctcaaccg gactagggt ctacaatgg 
      cagccagcg attcagcag gcgacgatt ccttgtacc 
      ccatcgccg tcctaatcg acgagctca aaaacgagg 
      atgtccagc tccgactca actccatca agaagctct 
      ccacgatcg cattggccc tgggggtgg agcgcaccc 
      gctccgagc tcatcccct tcctcacgg agaccatct 
      acgacgagg acgaggtgc tcctagccc ttgccgagc 
      aactgggga gtttcatca cgttagttg gcgggcctg 
      aatacgcct actgtcttt taccgccgc ttgagagcc 
      tggccactg ttgaggaaa cagtcgtca gggacaagg 
      cagtggagt cgttgaggg cggtggcgc agcaacaca 
      gcccagctg atttagaaa accactttg tgcccctag 
      tccagagac tgtcagcag gggattggt ttacttcgc 
      ggacttcgg catgtgggc tattctccg tttgttacc 
      cccgcgtgt cggctcaaa tgaaggcgg atttgcgct 
      cgcattttc gggctttgt gtcaggacg acacgccga 
      tggttaggc gagctgccg ccacgaaat taggcgaat 
      tggcacaag tcgtcgagt tggagtatt taaaaaccg 
      atttaattc cgatgtttt tgagtctga cccaagatg 
      aacaagatt cagttaggc ttttggcag ttgaggctt 
      gtgtcagtt ttgcctcac tttttcaac aagaggata 
      tcgagatgt ttgtgatgc cgactgtta ggaattgca 
      ccggtgata catcatggc gtgtgcgct acatggttg 
      cggacaaat tcaccgatt tgcaaaagg ccgtaggac 
      cagaaatca cccgaaccg atttagtcc ccgcctttc 
      agaacctcc tcaaggaca ctgaagccg aagttcggg 
      ccgcagccg ccaataaag tcaaagatt tctgtcaaa 
      acttggaca aagctcatc aggagagca tcattatga 
      acaatatcc ttccctgcg ttaaagaac tcgtcgccg 
      atcctaacc aacatgtca agtcggcct tggcttcgg 
      tgataatgg gcctgagcc ctattctcg gccgtcaca 
      atactatcg aacatctcc ttcctttgt ttttgactc 
      aattgaaag acgaatgtc ctgaagttc gcttgaata 
      tcatctcga atctagatt gcgtgaatg aggtcatag 
      gaatccagc aattgtctc agagtttat tgccggcca 
      tcgtcgaat tggccgagg attcaaaat ggcgcgtcc 
      gttcggcca tcatcgaat acatgccat tgttggctg 
      gccaattgg gacgcgagt ttttcgatg agaaattaa 
      acgcactgt gcatgactt ggttgatgg atcatgtgt 
      tcgctattc gtgaagcgg caactttga atttgagga 
      agttggtgg atcagtttg gggctgaat gggccgaaa 
      ccacgatca ttccgaagg ttttggcaa tgtcccgtg 
      accagaatt atttgtata ggatgactt gtctgtttt 
      gcatcaatg tgttggcag aggcctgcg gtagcgata 
      ttacgacgc gattgttgt taccaactg ttctgagta 
      tggcgaatg ataaagtcg ccaatgtaa ggtttaacg 
      tcgctaaaa cgttacaaa aaatcgcac cgcagttgg 
      accaagcgg tcatccagc cccaagtta agcctgttt 
      tagacaaat taacagcgg atagcgata tcgatgtca 
      agtattcgg cgtccgaag ccatcgctg ggattgctg 
      cgttaggtt gaagaggcg tttgcgcaa gagctgaaa 
      aaaagtgaa aggatctga aaatataca tacaacata 
      ataaattaa tctcgtaga ttgtaatat tatatattt 
      gttgtgctc catatgatt taaaacaat gtgtaatat 
      agcgtcttt agaaaaaaa acctataaa aacctgttc 
      actatttcg tcactttat cctttttat actttactt 
      tttttgtaa ttgttttcg aagcgtttt ttagttaat 
      tgttttagt attacatga taaggaaac tcggccctg 
      atccggttt cggattagt tttactctt cttttgtat 
      taattaaag aataaacaa tttcaaaca ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY