Status
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Gene information
General information
TC011972 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9591269..9615043
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011972
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06471

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011972 RA
      MNKSPPTDC NGPIQMSEV SVIDNKAWK CSEANNGYL 
      KQKSVPEES LLGSTAVTT VVQVSGAGT KTMVVPRNK 
      QAKDKCLVA AVFALIIFS LVLLFMVFS TRNHQCIDS 
      RSSVCLTEE CVRTAASLL GAMDHTADP CVDFFQYAC 
      GTWNKKHVI PEDRSSIST FEVMADQLQ VILKGVLEE 
      PINLEDNEA TRKAKTFYN TCMDILQIR KIGDAPLRE 
      VLEGLGGWP VTKPDWTPP SFSIEVLLG KIRGVYNEG 
      VLIEQWVGP DDKNSSVNI IQMDQMQLA LPSRDYYLK 
      ASSEGDMTA YHKYMTNIA VLLGANKTT AKEELQEVV 
      DFERRLANA SLPEADRHD TSAIYRKLT LQELQTIVP 
      QIKWLEYLR SFLDVDIDD QEPVVAYGL SYFIEMGKI 
      LAETNQRVI HNYVLWRLI MSLSPHMID DYQKERVEF 
      RKILQGVLS ERHRWSQCV EWTNKKLGM AVGALFIRD 
      NFNHDSKET ALTMIHTIR EAFNELLAE NDWMDDETR 
      AVAKEKADA MNERIGYPQ LITNKQELV KEYAALNIT 
      KTEFITNVL NILKYDAEQ NLQKLRQPV DKDKWSTEP 
      AVVNAFYNP NKNDIVFPA GILQPLFYS QHFPKSLNY 
      GGIGVVIGH EITHGFDDK GRQFDKDGN MMQWWNNAT 
      IRAFRERTQ CIIDQYSRY KIDEVGLYV NGRMTQGEN 
      IADNGGLKQ SFRAYRKWV SQHGEEPDL PGLNLTHDQ 
      LFFLNYAQI WCGSMRPED ALTKVRSSV HSPGPIRVL 
      GPLSNSWDF AKAYDCPLG SPMNPTNKC SVW
    CDS sequence
    • >TC011972 RA
      atgaataag tcacccccc actgactgt aatggacca 
      atccaaatg agtgaagtg tctgtgata gacaataaa 
      gcgtggaaa tgttcggaa gcgaacaac ggctaccta 
      aagcaaaaa agcgttcct gaagagtcg ctcctcggc 
      tccaccgcc gtgacgact gtcgtccaa gtctccgga 
      gccggaacc aaaaccatg gtggttccc cggaataaa 
      caagccaaa gacaaatgc cttgttgcg gccgttttc 
      gcactcatc attttctct ctagtctta ctcttcatg 
      gtgttcagc actcgaaac caccaatgt atcgatagc 
      agaagtagc gtttgtctt acggaggaa tgcgtgagg 
      acagcggcg tcccttttg ggggccatg gaccacacg 
      gcggacccc tgcgtggac tttttccaa tacgcctgt 
      ggtacgtgg aacaagaag cacgtgatc cccgaagac 
      cgcagctca atcagcacg ttcgaagta atggcagac 
      cagctccaa gtgatcctg aaaggcgtc ctcgaggag 
      cccattaac ctcgaagac aacgaggcg acacgcaaa 
      gccaaaacc ttctacaac acgtgcatg gatattctc 
      cagattcgg aaaattggg gacgcccca ctacgggag 
      gttctggag gggcttggg ggctggccg gtgaccaaa 
      ccagactgg actccgccc agcttctcc atcgaggtg 
      cttctgggg aagatcaga ggggtgtac aacgagggg 
      gtgctcatc gagcagtgg gtgggcccg gacgacaag 
      aacagctca gttaacatc atccagatg gaccaaatg 
      cagttggcg ttgcccagt cgcgattac tatcttaag 
      gcgagcagt gagggagac atgactgct tatcacaag 
      tatatgacg aacattgct gtcctcctt ggtgccaat 
      aaaactaca gccaaggaa gagctacaa gaagtggtc 
      gattttgaa cgaagactg gcaaatgcg tccttaccg 
      gaagctgac cgccatgac acttccgcc atttaccga 
      aaactaact ttacaagaa ttacaaaca atcgtccct 
      caaatcaaa tggctggaa tacctccgt tctttcctc 
      gacgttgac atcgacgac caagagccc gtcgttgct 
      tacggactt tcctatttc atcgaaatg ggaaaaatc 
      ctcgccgag acaaaccag agagttatt cacaattat 
      gtcctgtgg cgtctcatt atgtccctg tcgcctcac 
      atgattgac gattatcag aaggaacgc gtcgagttt 
      cgaaaaatt ctccaagga gtgctcagt gagcggcac 
      cgatggtcc caatgcgtc gagtggact aataaaaaa 
      cttggaatg gctgttgga gcacttttc atcagggat 
      aacttcaat cacgatagc aaagaaact gcacttacg 
      atgatacat acgatccga gaggcgttt aatgagctc 
      ttggctgag aatgattgg atggatgac gaaacgaga 
      gccgtggca aaggaaaag gcagatgct atgaatgag 
      aggattggg tatccgcaa ttaatcacg aacaagcag 
      gagttggtc aaggagtac gctgctttg aatattacc 
      aaaaccgag ttcattacg aacgtttta aacattcta 
      aaatacgac gccgagcag aatctgcaa aaattgaga 
      cagccagtc gataaagac aaatggtcg acggaaccg 
      gcggtcgtt aacgccttc tacaacccg aacaagaac 
      gatatcgtg tttcccgcc gggatcctg caaccgctc 
      ttctacagt cagcatttt cccaaatct ctgaattac 
      ggcggaatt ggagtggtc ataggtcac gaaataacg 
      cacggtttc gacgataaa ggccggcag tttgataag 
      gatgggaat atgatgcaa tggtggaat aacgcgacg 
      atccgggct ttccgggag cgaacccaa tgcataatt 
      gaccagtac tcgcgatac aaaattgat gaagtgggg 
      ctctacgtc aacgggcgc atgacccaa ggcgagaac 
      atcgccgac aacggcgga ctgaagcag tcattccgg 
      gcgtaccgt aaatgggtg tcccagcac ggggaagag 
      ccggacttg cccggtttg aacctaacc catgaccag 
      ctgtttttc ctcaactac gcccagatc tggtgcggg 
      tctatgcgg ccagaggat gcactgacc aaagtccgc 
      tccagcgtg cactcgccc ggacccatc cgggtgttg 
      ggtcctctc tccaactcg tgggatttc gccaaagct 
      tacgattgc ccgctcgga tcgccgatg aacccgact 
      aataaatgt agcgtttgg taa
    mRNA sequence
    • >TC011972 RA
      tgacgcagt ttaggtgct cttggcgaa actaaatcg 
      ataaagacg cggcggtcg tgcttcaca ctcgatgat 
      ttgctgccg tggggtata gattttccg actgctttt 
      tagctcttt tattgatgg tgaaacggg gaaatttgg 
      ggacaaaga gggccgaaa agacataac ccgcttggg 
      gaagaaaat tcgactttg tttgtgttg gtggtcgac 
      ggttcggga cacgtgtct cggaatttt ttcattttc 
      aaaaataat tcagtttaa tcaaatttg agttttgat 
      gagcaaaaa atgacttgc tgtcagtcg ccctatacg 
      tagcttcgg cccccatga cttaacccc cgtcccccg 
      atgaataag tcacccccc actgactgt aatggacca 
      atccaaatg agtgaagtg tctgtgata gacaataaa 
      gcgtggaaa tgttcggaa gcgaacaac ggctaccta 
      aagcaaaaa agcgttcct gaagagtcg ctcctcggc 
      tccaccgcc gtgacgact gtcgtccaa gtctccgga 
      gccggaacc aaaaccatg gtggttccc cggaataaa 
      caagccaaa gacaaatgc cttgttgcg gccgttttc 
      gcactcatc attttctct ctagtctta ctcttcatg 
      gtgttcagc actcgaaac caccaatgt atcgatagc 
      agaagtagc gtttgtctt acggaggaa tgcgtgagg 
      acagcggcg tcccttttg ggggccatg gaccacacg 
      gcggacccc tgcgtggac tttttccaa tacgcctgt 
      ggtacgtgg aacaagaag cacgtgatc cccgaagac 
      cgcagctca atcagcacg ttcgaagta atggcagac 
      cagctccaa gtgatcctg aaaggcgtc ctcgaggag 
      cccattaac ctcgaagac aacgaggcg acacgcaaa 
      gccaaaacc ttctacaac acgtgcatg gatattctc 
      cagattcgg aaaattggg gacgcccca ctacgggag 
      gttctggag gggcttggg ggctggccg gtgaccaaa 
      ccagactgg actccgccc agcttctcc atcgaggtg 
      cttctgggg aagatcaga ggggtgtac aacgagggg 
      gtgctcatc gagcagtgg gtgggcccg gacgacaag 
      aacagctca gttaacatc atccagatg gaccaaatg 
      cagttggcg ttgcccagt cgcgattac tatcttaag 
      gcgagcagt gagggagac atgactgct tatcacaag 
      tatatgacg aacattgct gtcctcctt ggtgccaat 
      aaaactaca gccaaggaa gagctacaa gaagtggtc 
      gattttgaa cgaagactg gcaaatgcg tccttaccg 
      gaagctgac cgccatgac acttccgcc atttaccga 
      aaactaact ttacaagaa ttacaaaca atcgtccct 
      caaatcaaa tggctggaa tacctccgt tctttcctc 
      gacgttgac atcgacgac caagagccc gtcgttgct 
      tacggactt tcctatttc atcgaaatg ggaaaaatc 
      ctcgccgag acaaaccag agagttatt cacaattat 
      gtcctgtgg cgtctcatt atgtccctg tcgcctcac 
      atgattgac gattatcag aaggaacgc gtcgagttt 
      cgaaaaatt ctccaagga gtgctcagt gagcggcac 
      cgatggtcc caatgcgtc gagtggact aataaaaaa 
      cttggaatg gctgttgga gcacttttc atcagggat 
      aacttcaat cacgatagc aaagaaact gcacttacg 
      atgatacat acgatccga gaggcgttt aatgagctc 
      ttggctgag aatgattgg atggatgac gaaacgaga 
      gccgtggca aaggaaaag gcagatgct atgaatgag 
      aggattggg tatccgcaa ttaatcacg aacaagcag 
      gagttggtc aaggagtac gctgctttg aatattacc 
      aaaaccgag ttcattacg aacgtttta aacattcta 
      aaatacgac gccgagcag aatctgcaa aaattgaga 
      cagccagtc gataaagac aaatggtcg acggaaccg 
      gcggtcgtt aacgccttc tacaacccg aacaagaac 
      gatatcgtg tttcccgcc gggatcctg caaccgctc 
      ttctacagt cagcatttt cccaaatct ctgaattac 
      ggcggaatt ggagtggtc ataggtcac gaaataacg 
      cacggtttc gacgataaa ggccggcag tttgataag 
      gatgggaat atgatgcaa tggtggaat aacgcgacg 
      atccgggct ttccgggag cgaacccaa tgcataatt 
      gaccagtac tcgcgatac aaaattgat gaagtgggg 
      ctctacgtc aacgggcgc atgacccaa ggcgagaac 
      atcgccgac aacggcgga ctgaagcag tcattccgg 
      gcgtaccgt aaatgggtg tcccagcac ggggaagag 
      ccggacttg cccggtttg aacctaacc catgaccag 
      ctgtttttc ctcaactac gcccagatc tggtgcggg 
      tctatgcgg ccagaggat gcactgacc aaagtccgc 
      tccagcgtg cactcgccc ggacccatc cgggtgttg 
      ggtcctctc tccaactcg tgggatttc gccaaagct 
      tacgattgc ccgctcgga tcgccgatg aacccgact 
      aataaatgt agcgtttgg taaaatggg actttcacg 
      cgctctatg tcaagtgtc tcgttgtat ttgcaaaaa 
      tttaacact ttcatctat attatcgcc gggcctttg 
      tagcggggc ccacaccta acttgtgca atgtaaata 
      ttattgttt tatataaaa ctgtacgca attttacat 
      gtaataaac ctacttttc ctattctaa acatggtct 
      gtactaata tacaaagta tgtattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY