| General information | |||
| TC012484 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 9180613..9195717 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012484 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06469 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 6 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC012484 RA
MQQPVIEEG AAYEPSVRS YDDLFPALP ESNTQSQNH NTMGQWSNK MRVGSSVIT QVFRVPFEE RKLDGSQKF GEGESIQTC ANIMKETGA HIEISHAKD QSLTFLVTG KQNEVLEAR RKILTHFQT QASKQIAIP KEHHRWILG KKGDRLKEL EKQTATKIS VPPMNDPSD IISITGTKE GIEKAEHEI KVTSDQQSK KASERINVP KIYHPFIVG PYNDYLNQL IAETGAKIN VPPPSVMKD EIFIAGEKE GVLAAKAKI EAIYKQMEK KCTTVSVEV PKTQHKYVI GPKGATIAE ILQTTGVSV EMPQGDSAT GTITLRGPH DKLGLALSK VYEKANSVR SSDVEAPSW IHKYIIGRK GQNIKEITQ NLPKVHVEF TEKEDKIKI EGPPEEVEK AQEQIEKMA KDLIKKLIF IEMHVDPKL FKHIIGKNG ANVNRLKEE FAVVINIDE SGLIRIEGH KEGVLTTKQ ELEDRIKKL ENEKEKDVI IEQRHYKSI IGAKGENIK EIREKFNQV QIYFPGAGD KNDIVKVRG PKEDVDKCC RHLEKLVKE LNEQSYQIE VPIYKQFHK FIIGKGGAN IRKIREETH TKIDLPAEG DKNDVITIT GKKEDVEEA REKIRKIQD ELENIVSEE ITIPPKFYN SLIGAKGKL IHSIMEDCG GVAIKFPSA DSKSDKVTI RGPKDDVDR AKQQLLDLA NERQLASYT AEVRAKAQH HKFLIGKNG ANIKKIRDS TGARIVFPS STDDDREII TIIGKKEAV EEAKAALQA TIKDIDNII ESEMSVEPR HHKHFVARR GEVLHKISD ECGGVMISF PRSGVDSDR VVLKGSKEC IEAAKQRIN EIIQDLESM ITIECVIPQ NHHRTVMGA KGFKVQGIT SDFDVQIKF PDRENTEEY PGHGQLNGD INGGPVRQC DVIRITGKE ENCLKAKQA LLDLVPVTI SVDVPYDLH RSIIGQKGR DVKELMDRY DVHIVLSPT GVKEDVIKI TGTPSNVER AKEALLEKV QELEADRKD RELKAFALK IEVNPEYHP KIIGKRGAV ITKIRKDHD VQIIFPKKG DPDEQIITI TGYEENTHR AKEDIMKIV NELNELVRE EVQIDSRVH SRIIGARGR NVRKIMEDY RVDIKFPRS EDADPNLVI ITGHEENVV DAKEHLISL EEQYLEDVA DQEDREKQH TLSFHFDSM TGGRSRDPN SNGFVVQGG PWEQKAPNT ASVTEFPSF GRNTEEPQA SPISGAWGS RR
- >TC012484 RA
atgcagcaa ccagtgatt gaagagggt gcggcttac gagccttcc gtaagaagc tacgatgat ctatttcca gctttgccc gaatcgaac acacaaagt caaaatcac aacactatg ggacaatgg agtaataaa atgagagtc ggttcgtcg gtcatcact caagtgttc cgggtacca ttcgaagag cgcaagctg gatggcagt caaaaattc ggcgagggc gaatctatc cagacctgc gcgaacatc atgaaagag accggagct cacatcgaa atctcccac gcgaaagac caatcgctc acattttta gtaaccgga aagcagaac gaagtcttg gaagcccgc cgcaaaata ctaacccat ttccaaacc caagccagc aaacaaata gccatcccg aaagaacac caccgctgg atcttaggc aagaaaggc gatcgtctc aaagaatta gaaaaacaa acggctact aaaatctcc gtgccgccc atgaacgac ccatcggac atcatcagc atcactggg acgaaagag ggaatcgaa aaggccgaa cacgagatt aaagtcacc tcagaccag cagtcgaag aaggcatca gaacgaatc aacgtcccg aaaatctat catccgttt attgtgggg ccttacaat gactatctt aaccaacta atcgcggaa accggggct aaaatcaac gtcccccca ccgtccgta atgaaagac gagattttt atcgccgga gagaaagag ggcgtctta gccgccaaa gctaaaatc gaagccatc tacaaacaa atggagaag aaatgcacg acggtttca gtcgaagtt cctaaaacg cagcacaaa tacgtcatc gggcctaaa ggggcgacg atagctgag attttacaa acgacaggt gtgagtgtc gagatgcct caaggcgat tcggccact ggaacaatc actctgaga ggacctcac gataagctg ggtctggcc ttgagcaaa gtctacgaa aaagccaac tcggtgcga tcgagcgat gtcgaagcg ccatcttgg attcacaag tacatcatc gggcggaaa gggcaaaac attaaagag attacgcag aatttgccc aaagtacac gtcgagttc accgaaaaa gaagacaag atcaagatt gaaggacca ccagaagag gtggagaag gcgcaagaa caaatcgaa aagatggcc aaggatttg atcaagaag cttatcttt atcgagatg cacgtcgat ccgaagctc ttcaagcat atcattggg aaaaatggc gcaaatgtg aatcgattg aaggaggag ttcgcagtc gtgatcaac atcgacgag agcggcctt attcgcatc gagggccac aaagaaggc gttttaacc acgaaacaa gagctcgag gaccgtatt aagaagctc gaaaatgag aaagaaaaa gacgtgata atcgagcaa cgccactac aagagcatc atcggcgcg aaaggcgag aatatcaaa gaaattcgt gaaaaattc aaccaagta caaatctat ttccctggt gctggggat aaaaacgac attgtcaaa gtccgcggt ccgaaagaa gacgtcgac aagtgttgc cgccattta gagaaatta gtcaaagaa ctgaacgaa cagtcatat caaatcgaa gtgccgatc tataaacag ttccacaag tttattatc ggcaaaggt ggggccaat atccggaaa atccgcgaa gagacacac acgaagatc gatttgccg gctgaaggt gacaaaaat gatgtgatt acgattacg ggtaagaaa gaagacgtg gaagaggcg cgcgagaag attaggaag attcaagat gagcttgaa aatatcgtc tcggaggag attacgatt cctccaaaa ttttacaat tcgttaatt ggggcgaaa gggaaactt attcattcg attatggag gattgcggt ggtgttgct atcaagttc ccgagtgcc gatagtaag agtgataag gtgaccatt agggggcct aaggatgat gtggatcgt gccaaacaa cagttgctt gatttagct aacgaacgc caacttgcc agttatacc gccgaggta cgtgccaaa gctcaacac cacaagttc ctgattggc aaaaacggt gccaatatt aagaaaatc cgcgattcg actggagct agaatcgta tttccttca agtaccgac gacgatcga gagattatc acaatcatc ggcaagaag gaggccgtg gaagaggct aaagcggcc ctccaagcc accatcaaa gatatcgac aatattatc gagagcgag atgtccgtg gaaccccgc caccacaag cacttcgtg gcgcgtcgc ggcgaagtc ctccacaag atcagcgac gaatgcggc ggtgtgatg atctccttc cctcgttcg ggcgtcgac agcgaccgc gtcgtcctc aaaggctcc aaggagtgc atcgaggcg gcgaagcaa cgcatcaac gaaatcatc caagacctc gaatcgatg atcaccatc gaatgcgtc atcccgcag aaccaccac cgcaccgtc atgggcgcc aaaggcttc aaggtgcaa ggcatcacc tccgacttc gacgtccag atcaaattc cccgatcgg gagaacacg gaagaatac cccggccat gggcagttg aacggcgac atcaacggc gggccggtg cgccaatgc gacgtcatc cgcatcacc ggtaaagaa gaaaactgc cttaaggcg aagcaagct ctcttggat ttggtcccg gtgaccatc agcgtggac gtgccctat gacttgcac aggtcgatc attgggcaa aaaggccgc gatgtgaag gaattgatg gacagatac gatgtccat attgtcctg tcgccgact ggagtcaag gaagatgtg attaaaatc accggaacg cccagtaat gtcgagcgg gctaaagag gcgttgcta gaaaaggtg caagagttg gaagccgat aggaaagac cgtgaattg aaggcgttt gcgttgaag attgaagtc aatcctgag tatcatccg aagattatc ggcaaacgt ggtgccgtt atcacgaaa attaggaag gatcatgac gtgcagatt attttccct aaaaaaggt gatcccgac gagcagata ataaccata accgggtac gaagagaac acccacagg gcaaaagag gacataatg aaaatcgtc aacgaactc aacgaactc gtccgcgag gaagtccaa atcgactcg cgagtccac tcacgtatc ataggcgct cgcggtcga aacgtccgc aaaataatg gaagattac agagtggac ataaaattc cctcgttct gaagacgct gatccgaat ctcgtcatc ataaccggc cacgaggag aacgtcgtg gacgccaaa gagcacttg atcagcctg gaagaacaa tacttggaa gacgtcgca gatcaagaa gaccgagag aaacaacac accttgagc tttcacttc gattctatg acaggtggc cgttctcgc gatcccaac agcaatggc tttgtggtt caagggggc ccttgggag cagaaggcc ccgaacacc gccagcgtg accgaattt ccctcgttc ggacgcaac accgaggag cctcaagcc tcccccatc tcgggggct tgggggagt cgccgttaa
- >TC012484 RA
caaatgtca gaaattgcc acttgcctt gccagccca tcattttga tttcatttc gtgcttggc agtcggtat gagtggagt gtttggtgt catcccttg cttctaatt aaatattta ctgttccag cgagacata accaagtaa aactggagg aatttagga gatccaact ttttctagg acggatttt ctgttttta cagtacatg ttgctgtaa cgacttggc acgacacag gacaatccg aaaatgcag caaccagtg attgaagag ggtgcggct tacgagcct tccgtaaga agctacgat gatctattt ccagctttg cccgaatcg aacacacaa agtcaaaat cacaacact atgggacaa tggagtaat aaaatgaga gtcggttcg tcggtcatc actcaagtg ttccgggta ccattcgaa gagcgcaag ctggatggc agtcaaaaa ttcggcgag ggcgaatct atccagacc tgcgcgaac atcatgaaa gagaccgga gctcacatc gaaatctcc cacgcgaaa gaccaatcg ctcacattt ttagtaacc ggaaagcag aacgaagtc ttggaagcc cgccgcaaa atactaacc catttccaa acccaagcc agcaaacaa atagccatc ccgaaagaa caccaccgc tggatctta ggcaagaaa ggcgatcgt ctcaaagaa ttagaaaaa caaacggct actaaaatc tccgtgccg cccatgaac gacccatcg gacatcatc agcatcact gggacgaaa gagggaatc gaaaaggcc gaacacgag attaaagtc acctcagac cagcagtcg aagaaggca tcagaacga atcaacgtc ccgaaaatc tatcatccg tttattgtg gggccttac aatgactat cttaaccaa ctaatcgcg gaaaccggg gctaaaatc aacgtcccc ccaccgtcc gtaatgaaa gacgagatt tttatcgcc ggagagaaa gagggcgtc ttagccgcc aaagctaaa atcgaagcc atctacaaa caaatggag aagaaatgc acgacggtt tcagtcgaa gttcctaaa acgcagcac aaatacgtc atcgggcct aaaggggcg acgatagct gagatttta caaacgaca ggtgtgagt gtcgagatg cctcaaggc gattcggcc actggaaca atcactctg agaggacct cacgataag ctgggtctg gccttgagc aaagtctac gaaaaagcc aactcggtg cgatcgagc gatgtcgaa gcgccatct tggattcac aagtacatc atcgggcgg aaagggcaa aacattaaa gagattacg cagaatttg cccaaagta cacgtcgag ttcaccgaa aaagaagac aagatcaag attgaagga ccaccagaa gaggtggag aaggcgcaa gaacaaatc gaaaagatg gccaaggat ttgatcaag aagcttatc tttatcgag atgcacgtc gatccgaag ctcttcaag catatcatt gggaaaaat ggcgcaaat gtgaatcga ttgaaggag gagttcgca gtcgtgatc aacatcgac gagagcggc cttattcgc atcgagggc cacaaagaa ggcgtttta accacgaaa caagagctc gaggaccgt attaagaag ctcgaaaat gagaaagaa aaagacgtg ataatcgag caacgccac tacaagagc atcatcggc gcgaaaggc gagaatatc aaagaaatt cgtgaaaaa ttcaaccaa gtacaaatc tatttccct ggtgctggg gataaaaac gacattgtc aaagtccgc ggtccgaaa gaagacgtc gacaagtgt tgccgccat ttagagaaa ttagtcaaa gaactgaac gaacagtca tatcaaatc gaagtgccg atctataaa cagttccac aagtttatt atcggcaaa ggtggggcc aatatccgg aaaatccgc gaagagaca cacacgaag atcgatttg ccggctgaa ggtgacaaa aatgatgtg attacgatt acgggtaag aaagaagac gtggaagag gcgcgcgag aagattagg aagattcaa gatgagctt gaaaatatc gtctcggag gagattacg attcctcca aaattttac aattcgtta attggggcg aaagggaaa cttattcat tcgattatg gaggattgc ggtggtgtt gctatcaag ttcccgagt gccgatagt aagagtgat aaggtgacc attaggggg cctaaggat gatgtggat cgtgccaaa caacagttg cttgattta gctaacgaa cgccaactt gccagttat accgccgag gtacgtgcc aaagctcaa caccacaag ttcctgatt ggcaaaaac ggtgccaat attaagaaa atccgcgat tcgactgga gctagaatc gtatttcct tcaagtacc gacgacgat cgagagatt atcacaatc atcggcaag aaggaggcc gtggaagag gctaaagcg gccctccaa gccaccatc aaagatatc gacaatatt atcgagagc gagatgtcc gtggaaccc cgccaccac aagcacttc gtggcgcgt cgcggcgaa gtcctccac aagatcagc gacgaatgc ggcggtgtg atgatctcc ttccctcgt tcgggcgtc gacagcgac cgcgtcgtc ctcaaaggc tccaaggag tgcatcgag gcggcgaag caacgcatc aacgaaatc atccaagac ctcgaatcg atgatcacc atcgaatgc gtcatcccg cagaaccac caccgcacc gtcatgggc gccaaaggc ttcaaggtg caaggcatc acctccgac ttcgacgtc cagatcaaa ttccccgat cgggagaac acggaagaa taccccggc catgggcag ttgaacggc gacatcaac ggcgggccg gtgcgccaa tgcgacgtc atccgcatc accggtaaa gaagaaaac tgccttaag gcgaagcaa gctctcttg gatttggtc ccggtgacc atcagcgtg gacgtgccc tatgacttg cacaggtcg atcattggg caaaaaggc cgcgatgtg aaggaattg atggacaga tacgatgtc catattgtc ctgtcgccg actggagtc aaggaagat gtgattaaa atcaccgga acgcccagt aatgtcgag cgggctaaa gaggcgttg ctagaaaag gtgcaagag ttggaagcc gataggaaa gaccgtgaa ttgaaggcg tttgcgttg aagattgaa gtcaatcct gagtatcat ccgaagatt atcggcaaa cgtggtgcc gttatcacg aaaattagg aaggatcat gacgtgcag attattttc cctaaaaaa ggtgatccc gacgagcag ataataacc ataaccggg tacgaagag aacacccac agggcaaaa gaggacata atgaaaatc gtcaacgaa ctcaacgaa ctcgtccgc gaggaagtc caaatcgac tcgcgagtc cactcacgt atcataggc gctcgcggt cgaaacgtc cgcaaaata atggaagat tacagagtg gacataaaa ttccctcgt tctgaagac gctgatccg aatctcgtc atcataacc ggccacgag gagaacgtc gtggacgcc aaagagcac ttgatcagc ctggaagaa caatacttg gaagacgtc gcagatcaa gaagaccga gagaaacaa cacaccttg agctttcac ttcgattct atgacaggt ggccgttct cgcgatccc aacagcaat ggctttgtg gttcaaggg ggcccttgg gagcagaag gccccgaac accgccagc gtgaccgaa tttccctcg ttcggacgc aacaccgag gagcctcaa gcctccccc atctcgggg gcttggggg agtcgccgt taagtcatc aattcagct aagtctcta ctgtgattt gaactaatt ttttgtttg attttgaaa cattgtaac ggtttcatc aacttttcg tttcgggat tttttgtaa aatttggtg aaattgacc gcgagacaa aagtcactg ccaaaaaca agtagagaa ctgaagcta taattttcg aggttacgt taagtatac taacctagt agtaagaat aacagttgc tggtgcaac ctcttccct cactgtttg ctgcattca atgataggc ctggcgacg acactggac tctcgatat ggaaactga aatacacag acgcaagct atttctttt tgacgatat tatgtcttc cctgtggcg agcagacta ggcctagaa acggcacta atcacgtgg agatgttat gatatttac tgttttatt ttaactagc tgtacatga tccttttgt atattaatc tagttgatt taaacatat tatattgtt atgcatagt tggatatat tatatatat ttttaaagt tttatatat tttttcgtg tcataactt ctctgctct tcgtaacga ggcggtgca tctgtgcct tcctcattt ttatatgac cgtactgaa ctctcgtaa ggcacattt tgcattttc cggagcttt tccttaaaa gggtgaagg atttttttg ttttatgta tctattttt attatcatt aatcataag aaattcaac tacaagaaa cagactgaa atgtgccgc tgtttcggc actacttaa ttattttgt gttttgtga agtggaatt ttgctgtta ctgtatata attattata tgcacgagt ccttgcggt ccattttgt tagcccagg actaacctg agggagaga aatatatta atttaaaat aa
