Status
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Gene information
General information
TC012484 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9180613..9195717
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012484
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06469

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012484 RA
      MQQPVIEEG AAYEPSVRS YDDLFPALP ESNTQSQNH 
      NTMGQWSNK MRVGSSVIT QVFRVPFEE RKLDGSQKF 
      GEGESIQTC ANIMKETGA HIEISHAKD QSLTFLVTG 
      KQNEVLEAR RKILTHFQT QASKQIAIP KEHHRWILG 
      KKGDRLKEL EKQTATKIS VPPMNDPSD IISITGTKE 
      GIEKAEHEI KVTSDQQSK KASERINVP KIYHPFIVG 
      PYNDYLNQL IAETGAKIN VPPPSVMKD EIFIAGEKE 
      GVLAAKAKI EAIYKQMEK KCTTVSVEV PKTQHKYVI 
      GPKGATIAE ILQTTGVSV EMPQGDSAT GTITLRGPH 
      DKLGLALSK VYEKANSVR SSDVEAPSW IHKYIIGRK 
      GQNIKEITQ NLPKVHVEF TEKEDKIKI EGPPEEVEK 
      AQEQIEKMA KDLIKKLIF IEMHVDPKL FKHIIGKNG 
      ANVNRLKEE FAVVINIDE SGLIRIEGH KEGVLTTKQ 
      ELEDRIKKL ENEKEKDVI IEQRHYKSI IGAKGENIK 
      EIREKFNQV QIYFPGAGD KNDIVKVRG PKEDVDKCC 
      RHLEKLVKE LNEQSYQIE VPIYKQFHK FIIGKGGAN 
      IRKIREETH TKIDLPAEG DKNDVITIT GKKEDVEEA 
      REKIRKIQD ELENIVSEE ITIPPKFYN SLIGAKGKL 
      IHSIMEDCG GVAIKFPSA DSKSDKVTI RGPKDDVDR 
      AKQQLLDLA NERQLASYT AEVRAKAQH HKFLIGKNG 
      ANIKKIRDS TGARIVFPS STDDDREII TIIGKKEAV 
      EEAKAALQA TIKDIDNII ESEMSVEPR HHKHFVARR 
      GEVLHKISD ECGGVMISF PRSGVDSDR VVLKGSKEC 
      IEAAKQRIN EIIQDLESM ITIECVIPQ NHHRTVMGA 
      KGFKVQGIT SDFDVQIKF PDRENTEEY PGHGQLNGD 
      INGGPVRQC DVIRITGKE ENCLKAKQA LLDLVPVTI 
      SVDVPYDLH RSIIGQKGR DVKELMDRY DVHIVLSPT 
      GVKEDVIKI TGTPSNVER AKEALLEKV QELEADRKD 
      RELKAFALK IEVNPEYHP KIIGKRGAV ITKIRKDHD 
      VQIIFPKKG DPDEQIITI TGYEENTHR AKEDIMKIV 
      NELNELVRE EVQIDSRVH SRIIGARGR NVRKIMEDY 
      RVDIKFPRS EDADPNLVI ITGHEENVV DAKEHLISL 
      EEQYLEDVA DQEDREKQH TLSFHFDSM TGGRSRDPN 
      SNGFVVQGG PWEQKAPNT ASVTEFPSF GRNTEEPQA 
      SPISGAWGS RR
    CDS sequence
    • >TC012484 RA
      atgcagcaa ccagtgatt gaagagggt gcggcttac 
      gagccttcc gtaagaagc tacgatgat ctatttcca 
      gctttgccc gaatcgaac acacaaagt caaaatcac 
      aacactatg ggacaatgg agtaataaa atgagagtc 
      ggttcgtcg gtcatcact caagtgttc cgggtacca 
      ttcgaagag cgcaagctg gatggcagt caaaaattc 
      ggcgagggc gaatctatc cagacctgc gcgaacatc 
      atgaaagag accggagct cacatcgaa atctcccac 
      gcgaaagac caatcgctc acattttta gtaaccgga 
      aagcagaac gaagtcttg gaagcccgc cgcaaaata 
      ctaacccat ttccaaacc caagccagc aaacaaata 
      gccatcccg aaagaacac caccgctgg atcttaggc 
      aagaaaggc gatcgtctc aaagaatta gaaaaacaa 
      acggctact aaaatctcc gtgccgccc atgaacgac 
      ccatcggac atcatcagc atcactggg acgaaagag 
      ggaatcgaa aaggccgaa cacgagatt aaagtcacc 
      tcagaccag cagtcgaag aaggcatca gaacgaatc 
      aacgtcccg aaaatctat catccgttt attgtgggg 
      ccttacaat gactatctt aaccaacta atcgcggaa 
      accggggct aaaatcaac gtcccccca ccgtccgta 
      atgaaagac gagattttt atcgccgga gagaaagag 
      ggcgtctta gccgccaaa gctaaaatc gaagccatc 
      tacaaacaa atggagaag aaatgcacg acggtttca 
      gtcgaagtt cctaaaacg cagcacaaa tacgtcatc 
      gggcctaaa ggggcgacg atagctgag attttacaa 
      acgacaggt gtgagtgtc gagatgcct caaggcgat 
      tcggccact ggaacaatc actctgaga ggacctcac 
      gataagctg ggtctggcc ttgagcaaa gtctacgaa 
      aaagccaac tcggtgcga tcgagcgat gtcgaagcg 
      ccatcttgg attcacaag tacatcatc gggcggaaa 
      gggcaaaac attaaagag attacgcag aatttgccc 
      aaagtacac gtcgagttc accgaaaaa gaagacaag 
      atcaagatt gaaggacca ccagaagag gtggagaag 
      gcgcaagaa caaatcgaa aagatggcc aaggatttg 
      atcaagaag cttatcttt atcgagatg cacgtcgat 
      ccgaagctc ttcaagcat atcattggg aaaaatggc 
      gcaaatgtg aatcgattg aaggaggag ttcgcagtc 
      gtgatcaac atcgacgag agcggcctt attcgcatc 
      gagggccac aaagaaggc gttttaacc acgaaacaa 
      gagctcgag gaccgtatt aagaagctc gaaaatgag 
      aaagaaaaa gacgtgata atcgagcaa cgccactac 
      aagagcatc atcggcgcg aaaggcgag aatatcaaa 
      gaaattcgt gaaaaattc aaccaagta caaatctat 
      ttccctggt gctggggat aaaaacgac attgtcaaa 
      gtccgcggt ccgaaagaa gacgtcgac aagtgttgc 
      cgccattta gagaaatta gtcaaagaa ctgaacgaa 
      cagtcatat caaatcgaa gtgccgatc tataaacag 
      ttccacaag tttattatc ggcaaaggt ggggccaat 
      atccggaaa atccgcgaa gagacacac acgaagatc 
      gatttgccg gctgaaggt gacaaaaat gatgtgatt 
      acgattacg ggtaagaaa gaagacgtg gaagaggcg 
      cgcgagaag attaggaag attcaagat gagcttgaa 
      aatatcgtc tcggaggag attacgatt cctccaaaa 
      ttttacaat tcgttaatt ggggcgaaa gggaaactt 
      attcattcg attatggag gattgcggt ggtgttgct 
      atcaagttc ccgagtgcc gatagtaag agtgataag 
      gtgaccatt agggggcct aaggatgat gtggatcgt 
      gccaaacaa cagttgctt gatttagct aacgaacgc 
      caacttgcc agttatacc gccgaggta cgtgccaaa 
      gctcaacac cacaagttc ctgattggc aaaaacggt 
      gccaatatt aagaaaatc cgcgattcg actggagct 
      agaatcgta tttccttca agtaccgac gacgatcga 
      gagattatc acaatcatc ggcaagaag gaggccgtg 
      gaagaggct aaagcggcc ctccaagcc accatcaaa 
      gatatcgac aatattatc gagagcgag atgtccgtg 
      gaaccccgc caccacaag cacttcgtg gcgcgtcgc 
      ggcgaagtc ctccacaag atcagcgac gaatgcggc 
      ggtgtgatg atctccttc cctcgttcg ggcgtcgac 
      agcgaccgc gtcgtcctc aaaggctcc aaggagtgc 
      atcgaggcg gcgaagcaa cgcatcaac gaaatcatc 
      caagacctc gaatcgatg atcaccatc gaatgcgtc 
      atcccgcag aaccaccac cgcaccgtc atgggcgcc 
      aaaggcttc aaggtgcaa ggcatcacc tccgacttc 
      gacgtccag atcaaattc cccgatcgg gagaacacg 
      gaagaatac cccggccat gggcagttg aacggcgac 
      atcaacggc gggccggtg cgccaatgc gacgtcatc 
      cgcatcacc ggtaaagaa gaaaactgc cttaaggcg 
      aagcaagct ctcttggat ttggtcccg gtgaccatc 
      agcgtggac gtgccctat gacttgcac aggtcgatc 
      attgggcaa aaaggccgc gatgtgaag gaattgatg 
      gacagatac gatgtccat attgtcctg tcgccgact 
      ggagtcaag gaagatgtg attaaaatc accggaacg 
      cccagtaat gtcgagcgg gctaaagag gcgttgcta 
      gaaaaggtg caagagttg gaagccgat aggaaagac 
      cgtgaattg aaggcgttt gcgttgaag attgaagtc 
      aatcctgag tatcatccg aagattatc ggcaaacgt 
      ggtgccgtt atcacgaaa attaggaag gatcatgac 
      gtgcagatt attttccct aaaaaaggt gatcccgac 
      gagcagata ataaccata accgggtac gaagagaac 
      acccacagg gcaaaagag gacataatg aaaatcgtc 
      aacgaactc aacgaactc gtccgcgag gaagtccaa 
      atcgactcg cgagtccac tcacgtatc ataggcgct 
      cgcggtcga aacgtccgc aaaataatg gaagattac 
      agagtggac ataaaattc cctcgttct gaagacgct 
      gatccgaat ctcgtcatc ataaccggc cacgaggag 
      aacgtcgtg gacgccaaa gagcacttg atcagcctg 
      gaagaacaa tacttggaa gacgtcgca gatcaagaa 
      gaccgagag aaacaacac accttgagc tttcacttc 
      gattctatg acaggtggc cgttctcgc gatcccaac 
      agcaatggc tttgtggtt caagggggc ccttgggag 
      cagaaggcc ccgaacacc gccagcgtg accgaattt 
      ccctcgttc ggacgcaac accgaggag cctcaagcc 
      tcccccatc tcgggggct tgggggagt cgccgttaa
    mRNA sequence
    • >TC012484 RA
      caaatgtca gaaattgcc acttgcctt gccagccca 
      tcattttga tttcatttc gtgcttggc agtcggtat 
      gagtggagt gtttggtgt catcccttg cttctaatt 
      aaatattta ctgttccag cgagacata accaagtaa 
      aactggagg aatttagga gatccaact ttttctagg 
      acggatttt ctgttttta cagtacatg ttgctgtaa 
      cgacttggc acgacacag gacaatccg aaaatgcag 
      caaccagtg attgaagag ggtgcggct tacgagcct 
      tccgtaaga agctacgat gatctattt ccagctttg 
      cccgaatcg aacacacaa agtcaaaat cacaacact 
      atgggacaa tggagtaat aaaatgaga gtcggttcg 
      tcggtcatc actcaagtg ttccgggta ccattcgaa 
      gagcgcaag ctggatggc agtcaaaaa ttcggcgag 
      ggcgaatct atccagacc tgcgcgaac atcatgaaa 
      gagaccgga gctcacatc gaaatctcc cacgcgaaa 
      gaccaatcg ctcacattt ttagtaacc ggaaagcag 
      aacgaagtc ttggaagcc cgccgcaaa atactaacc 
      catttccaa acccaagcc agcaaacaa atagccatc 
      ccgaaagaa caccaccgc tggatctta ggcaagaaa 
      ggcgatcgt ctcaaagaa ttagaaaaa caaacggct 
      actaaaatc tccgtgccg cccatgaac gacccatcg 
      gacatcatc agcatcact gggacgaaa gagggaatc 
      gaaaaggcc gaacacgag attaaagtc acctcagac 
      cagcagtcg aagaaggca tcagaacga atcaacgtc 
      ccgaaaatc tatcatccg tttattgtg gggccttac 
      aatgactat cttaaccaa ctaatcgcg gaaaccggg 
      gctaaaatc aacgtcccc ccaccgtcc gtaatgaaa 
      gacgagatt tttatcgcc ggagagaaa gagggcgtc 
      ttagccgcc aaagctaaa atcgaagcc atctacaaa 
      caaatggag aagaaatgc acgacggtt tcagtcgaa 
      gttcctaaa acgcagcac aaatacgtc atcgggcct 
      aaaggggcg acgatagct gagatttta caaacgaca 
      ggtgtgagt gtcgagatg cctcaaggc gattcggcc 
      actggaaca atcactctg agaggacct cacgataag 
      ctgggtctg gccttgagc aaagtctac gaaaaagcc 
      aactcggtg cgatcgagc gatgtcgaa gcgccatct 
      tggattcac aagtacatc atcgggcgg aaagggcaa 
      aacattaaa gagattacg cagaatttg cccaaagta 
      cacgtcgag ttcaccgaa aaagaagac aagatcaag 
      attgaagga ccaccagaa gaggtggag aaggcgcaa 
      gaacaaatc gaaaagatg gccaaggat ttgatcaag 
      aagcttatc tttatcgag atgcacgtc gatccgaag 
      ctcttcaag catatcatt gggaaaaat ggcgcaaat 
      gtgaatcga ttgaaggag gagttcgca gtcgtgatc 
      aacatcgac gagagcggc cttattcgc atcgagggc 
      cacaaagaa ggcgtttta accacgaaa caagagctc 
      gaggaccgt attaagaag ctcgaaaat gagaaagaa 
      aaagacgtg ataatcgag caacgccac tacaagagc 
      atcatcggc gcgaaaggc gagaatatc aaagaaatt 
      cgtgaaaaa ttcaaccaa gtacaaatc tatttccct 
      ggtgctggg gataaaaac gacattgtc aaagtccgc 
      ggtccgaaa gaagacgtc gacaagtgt tgccgccat 
      ttagagaaa ttagtcaaa gaactgaac gaacagtca 
      tatcaaatc gaagtgccg atctataaa cagttccac 
      aagtttatt atcggcaaa ggtggggcc aatatccgg 
      aaaatccgc gaagagaca cacacgaag atcgatttg 
      ccggctgaa ggtgacaaa aatgatgtg attacgatt 
      acgggtaag aaagaagac gtggaagag gcgcgcgag 
      aagattagg aagattcaa gatgagctt gaaaatatc 
      gtctcggag gagattacg attcctcca aaattttac 
      aattcgtta attggggcg aaagggaaa cttattcat 
      tcgattatg gaggattgc ggtggtgtt gctatcaag 
      ttcccgagt gccgatagt aagagtgat aaggtgacc 
      attaggggg cctaaggat gatgtggat cgtgccaaa 
      caacagttg cttgattta gctaacgaa cgccaactt 
      gccagttat accgccgag gtacgtgcc aaagctcaa 
      caccacaag ttcctgatt ggcaaaaac ggtgccaat 
      attaagaaa atccgcgat tcgactgga gctagaatc 
      gtatttcct tcaagtacc gacgacgat cgagagatt 
      atcacaatc atcggcaag aaggaggcc gtggaagag 
      gctaaagcg gccctccaa gccaccatc aaagatatc 
      gacaatatt atcgagagc gagatgtcc gtggaaccc 
      cgccaccac aagcacttc gtggcgcgt cgcggcgaa 
      gtcctccac aagatcagc gacgaatgc ggcggtgtg 
      atgatctcc ttccctcgt tcgggcgtc gacagcgac 
      cgcgtcgtc ctcaaaggc tccaaggag tgcatcgag 
      gcggcgaag caacgcatc aacgaaatc atccaagac 
      ctcgaatcg atgatcacc atcgaatgc gtcatcccg 
      cagaaccac caccgcacc gtcatgggc gccaaaggc 
      ttcaaggtg caaggcatc acctccgac ttcgacgtc 
      cagatcaaa ttccccgat cgggagaac acggaagaa 
      taccccggc catgggcag ttgaacggc gacatcaac 
      ggcgggccg gtgcgccaa tgcgacgtc atccgcatc 
      accggtaaa gaagaaaac tgccttaag gcgaagcaa 
      gctctcttg gatttggtc ccggtgacc atcagcgtg 
      gacgtgccc tatgacttg cacaggtcg atcattggg 
      caaaaaggc cgcgatgtg aaggaattg atggacaga 
      tacgatgtc catattgtc ctgtcgccg actggagtc 
      aaggaagat gtgattaaa atcaccgga acgcccagt 
      aatgtcgag cgggctaaa gaggcgttg ctagaaaag 
      gtgcaagag ttggaagcc gataggaaa gaccgtgaa 
      ttgaaggcg tttgcgttg aagattgaa gtcaatcct 
      gagtatcat ccgaagatt atcggcaaa cgtggtgcc 
      gttatcacg aaaattagg aaggatcat gacgtgcag 
      attattttc cctaaaaaa ggtgatccc gacgagcag 
      ataataacc ataaccggg tacgaagag aacacccac 
      agggcaaaa gaggacata atgaaaatc gtcaacgaa 
      ctcaacgaa ctcgtccgc gaggaagtc caaatcgac 
      tcgcgagtc cactcacgt atcataggc gctcgcggt 
      cgaaacgtc cgcaaaata atggaagat tacagagtg 
      gacataaaa ttccctcgt tctgaagac gctgatccg 
      aatctcgtc atcataacc ggccacgag gagaacgtc 
      gtggacgcc aaagagcac ttgatcagc ctggaagaa 
      caatacttg gaagacgtc gcagatcaa gaagaccga 
      gagaaacaa cacaccttg agctttcac ttcgattct 
      atgacaggt ggccgttct cgcgatccc aacagcaat 
      ggctttgtg gttcaaggg ggcccttgg gagcagaag 
      gccccgaac accgccagc gtgaccgaa tttccctcg 
      ttcggacgc aacaccgag gagcctcaa gcctccccc 
      atctcgggg gcttggggg agtcgccgt taagtcatc 
      aattcagct aagtctcta ctgtgattt gaactaatt 
      ttttgtttg attttgaaa cattgtaac ggtttcatc 
      aacttttcg tttcgggat tttttgtaa aatttggtg 
      aaattgacc gcgagacaa aagtcactg ccaaaaaca 
      agtagagaa ctgaagcta taattttcg aggttacgt 
      taagtatac taacctagt agtaagaat aacagttgc 
      tggtgcaac ctcttccct cactgtttg ctgcattca 
      atgataggc ctggcgacg acactggac tctcgatat 
      ggaaactga aatacacag acgcaagct atttctttt 
      tgacgatat tatgtcttc cctgtggcg agcagacta 
      ggcctagaa acggcacta atcacgtgg agatgttat 
      gatatttac tgttttatt ttaactagc tgtacatga 
      tccttttgt atattaatc tagttgatt taaacatat 
      tatattgtt atgcatagt tggatatat tatatatat 
      ttttaaagt tttatatat tttttcgtg tcataactt 
      ctctgctct tcgtaacga ggcggtgca tctgtgcct 
      tcctcattt ttatatgac cgtactgaa ctctcgtaa 
      ggcacattt tgcattttc cggagcttt tccttaaaa 
      gggtgaagg atttttttg ttttatgta tctattttt 
      attatcatt aatcataag aaattcaac tacaagaaa 
      cagactgaa atgtgccgc tgtttcggc actacttaa 
      ttattttgt gttttgtga agtggaatt ttgctgtta 
      ctgtatata attattata tgcacgagt ccttgcggt 
      ccattttgt tagcccagg actaacctg agggagaga 
      aatatatta atttaaaat aa
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