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Gene information
General information
TC012014 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8501855..8506599
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012014
    iBeetle screen iB_06460

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012014 RA
      MLRYCQKIK GFCPTFVKL RQIHNFSPD KNYDVIVVG 
      GGHAGSEAS AAAARMGAQ TLLVTHKKE TVGEMSCNP 
      SFGGIGKGH LMREIDALD GICGRMCDL SGIQYKVLN 
      KRKGPAVWG YRAQIDRDL YKKHIQTEL FENTPNLDI 
      LVASVEDLI VENPTTNSN KQTVIDCHG IILSDGTKI 
      HAKSVIITT GTFLNGQIN IGTKIYPAG RIGDQPSIG 
      LAKTLAALS LKMGRLKTG TPPRLNSNT INYKVCDIQ 
      KGDNPPLPF SFMNDKVWI KPEDQLVCY LTKTSLDIE 
      PIIRNNLHV NRHVTEEVT GPRYCPSIE SKVLRFGGR 
      QHQVWLEPE GLTSDIVYP NGLSCTLPE ELQFELIHS 
      IPGLEKAEI TRPGYGVEY DFVDPRELQ TTLELKRIH 
      NLFLAGQIN GTTGYEEAA AQGIIAGIN AAALALGKS 
      PITVSRTEG YIGVLIDDL TTLGTNEPY RMFTSRAEF 
      RLTLRPDNA DQRLTAKGY QVGCVNKAR YDKMVAMQK 
      KLNDGISLL KSITKPART WRKLLKMAP SRNMMQKSA 
      FNMLDCYNE GITISMLAT VAPELLPLT HDPLLENRL 
      HIEATYEAA ASEQAVEVE EVRKEEALL IPKDLDYYS 
      DTLNLNSEE REKLCSIQP QTIAAASRI PGISPSSVF 
      RLLRFVKKN PAVQAV
    CDS sequence
    • >TC012014 RA
      atgttgcgt tattgtcag aaaatcaag ggtttttgc 
      ccaacattt gtgaaactg cggcaaata cacaacttt 
      tctcctgat aaaaactac gacgttatt gttgtgggt 
      ggtggtcat gctggaagt gaggcaagt gctgcagcg 
      gcccgaatg ggggctcaa acacttctc gtaacgcac 
      aaaaaagaa accgttggg gaaatgtca tgcaacccc 
      tcatttggg ggcatcgga aaggggcat ttgatgcga 
      gaaatagac gccttggat gggatttgc ggccgaatg 
      tgcgacctg tccggtatt cagtataaa gtactcaat 
      aaacgcaaa ggacccgca gtttggggt taccgcgct 
      cagatcgac agagacctc tacaagaaa cacatccaa 
      accgaactt tttgaaaac acaccaaac ttagacatt 
      ttagtggct tcagtcgaa gacttaatc gtcgaaaat 
      ccgacaaca aacagtaac aaacaaacc gtaatagac 
      tgtcacggt atcattcta agtgatggc acaaaaatc 
      cacgctaaa agtgttata ataaccact ggtactttc 
      cttaatggt caaataaat atcggtact aaaatatac 
      ccagctggg cgcataggc gaccaaccg tcgatcggt 
      ttagccaaa actttagcc gccttgagt ctcaaaatg 
      gggcgtttg aaaacaggg actcccccg agacttaat 
      tcaaatacg ataaattat aaagtctgt gatatacaa 
      aagggtgat aacccgccg ttacctttc tcgtttatg 
      aatgataaa gtctggatt aagcctgaa gatcagttg 
      gtttgttac ctaactaaa acctcgctg gatattgaa 
      ccgattatt aggaataat cttcacgtt aaccggcat 
      gtaaccgag gaagtaacc ggtccgagg tattgtccg 
      agtatcgaa tcaaaagtc ttgcggttt ggggggcgc 
      cagcaccag gtttggtta gaaccggaa ggtctcact 
      agtgatata gtttaccca aatgggttg tcttgcact 
      ctcccggaa gaacttcaa tttgagttg attcattcg 
      ataccgggg ttagaaaag gcggaaatc acacgacct 
      gggtatggg gttgaatac gattttgtg gatccccgg 
      gaattgcaa actaccctt gaattgaaa cgaatccac 
      aatttattc ctcgctggt caaattaac gggactaca 
      ggatacgag gaagcggca gctcagggg ataatcgct 
      ggaataaat gccgcagca cttgctttg ggtaaatcc 
      cccattacg gtgagtcgt accgaaggt tacatcggg 
      gttttaatc gacgatttg acaacgctt ggaaccaac 
      gaaccgtac cgaatgttc acgagtcga gccgaattc 
      cgactaact ttaaggccg gacaatgcc gatcaaaga 
      ctcacagct aaaggttat caagtaggt tgtgtcaat 
      aaagctagg tatgacaaa atggtggcc atgcaaaaa 
      aaactaaat gatggaata tctcttttg aaaagtata 
      acaaaacca gctcgaact tggagaaaa cttttgaaa 
      atggcgcca tctaggaat atgatgcaa aagtcggct 
      tttaacatg cttgattgc tacaatgag gggattacg 
      atttcgatg ttggcaaca gtggcaccg gaattgttg 
      ccactaaca catgacccc ctacttgaa aatcgctta 
      catatcgaa gctacctat gaagctgca gcttctgaa 
      caagccgtt gaagtggag gaagtgcga aaggaagaa 
      gctttgctc attccgaaa gacttagac tattattca 
      gatactctt aatttgaat tccgaagag agggaaaag 
      ttgtgttct atacaacca caaactatt gctgccgct 
      agtcgaatt ccgggaatt agtccgtct tctgttttt 
      agattgttg cgttttgtt aaaaaaaat ccagcggtt 
      caagccgta taa
    mRNA sequence
    • >TC012014 RA
      tttctaacc tcacttttg cattaattt tatttagtg 
      atttcagta ctcctagta cgtccctgt aattattta 
      cataattag taaactaag tgggcaatt attagtaat 
      tgtacggta aaatcttaa aaaatatct acaatcctg 
      gaatttttt tttaacata acctaactt tcccatgtt 
      caaaatgtt gcgttattg tcagaaaat caagggttt 
      ttgcccaac atttgtgaa actgcggca aatacacaa 
      cttttctcc tgataaaaa ctacgacgt tattgttgt 
      gggtggtgg tcatgctgg aagtgaggc aagtgctgc 
      agcggcccg aatgggggc tcaaacact tctcgtaac 
      gcacaaaaa agaaaccgt tggggaaat gtcatgcaa 
      cccctcatt tgggggcat cggaaaggg gcatttgat 
      gcgagaaat agacgcctt ggatgggat ttgcggccg 
      aatgtgcga cctgtccgg tattcagta taaagtact 
      caataaacg caaaggacc cgcagtttg gggttaccg 
      cgctcagat cgacagaga cctctacaa gaaacacat 
      ccaaaccga actttttga aaacacacc aaacttaga 
      cattttagt ggcttcagt cgaagactt aatcgtcga 
      aaatccgac aacaaacag taacaaaca aaccgtaat 
      agactgtca cggtatcat tctaagtga tggcacaaa 
      aatccacgc taaaagtgt tataataac cactggtac 
      tttccttaa tggtcaaat aaatatcgg tactaaaat 
      atacccagc tgggcgcat aggcgacca accgtcgat 
      cggtttagc caaaacttt agccgcctt gagtctcaa 
      aatggggcg tttgaaaac agggactcc cccgagact 
      taattcaaa tacgataaa ttataaagt ctgtgatat 
      acaaaaggg tgataaccc gccgttacc tttctcgtt 
      tatgaatga taaagtctg gattaagcc tgaagatca 
      gttggtttg ttacctaac taaaacctc gctggatat 
      tgaaccgat tattaggaa taatcttca cgttaaccg 
      gcatgtaac cgaggaagt aaccggtcc gaggtattg 
      tccgagtat cgaatcaaa agtcttgcg gtttggggg 
      gcgccagca ccaggtttg gttagaacc ggaaggtct 
      cactagtga tatagttta cccaaatgg gttgtcttg 
      cactctccc ggaagaact tcaatttga gttgattca 
      ttcgatacc ggggttaga aaaggcgga aatcacacg 
      acctgggta tggggttga atacgattt tgtggatcc 
      ccgggaatt gcaaactac ccttgaatt gaaacgaat 
      ccacaattt attcctcgc tggtcaaat taacgggac 
      tacaggata cgaggaagc ggcagctca ggggataat 
      cgctggaat aaatgccgc agcacttgc tttgggtaa 
      atcccccat tacggtgag tcgtaccga aggttacat 
      cggggtttt aatcgacga tttgacaac gcttggaac 
      caacgaacc gtaccgaat gttcacgag tcgagccga 
      attccgact aactttaag gccggacaa tgccgatca 
      aagactcac agctaaagg ttatcaagt aggttgtgt 
      caataaagc taggtatga caaaatggt ggccatgca 
      aaaaaaact aaatgatgg aatatctct tttgaaaag 
      tataacaaa accagctcg aacttggag aaaactttt 
      gaaaatggc gccatctag gaatatgat gcaaaagtc 
      ggcttttaa catgcttga ttgctacaa tgaggggat 
      tacgatttc gatgttggc aacagtggc accggaatt 
      gttgccact aacacatga ccccctact tgaaaatcg 
      cttacatat cgaagctac ctatgaagc tgcagcttc 
      tgaacaagc cgttgaagt ggaggaagt gcgaaagga 
      agaagcttt gctcattcc gaaagactt agactatta 
      ttcagatac tcttaattt gaattccga agagaggga 
      aaagttgtg ttctataca accacaaac tattgctgc 
      cgctagtcg aattccggg aattagtcc gtcttctgt 
      ttttagatt gttgcgttt tgttaaaaa aaatccagc 
      ggttcaagc cgtataaaa tataaaatt aacgaaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY