Status
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Gene information
General information
TC012468 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8494731..8499517
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012468
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06459

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012468 RA
      MKKLHWFNV SSIGKPLKL FVTFALILT VLFITYFTY 
      LKPKPSPTK VPFQHRIFD EYKHMSHQE TPFKGAKIV 
      HLDLKGAPP KISYYKTLF SLLSKFGAT GVLIEYEDM 
      FPYNSPLLK NVSALNAYT VEDIHYINK LALENKLEV 
      IPLLQTFGH LEFLLKLEE FSELREVPE YPQVICPTH 
      EKTLSVLME MIDQVLQMH PHSKIIHIG ADEVYYLGF 
      CDRCANIMN KFNLSKNML FLEHINAIT TRIRKKHPH 
      LRILMWDDE FRSFSVKEL KSGFLDKGV QPVVWKYSK 
      DVYEELGPS LWEMYSEVF PKVWIASAF KGATGSNQY 
      VSDVTHYVQ NHRSWLSLM SEYSHRITF EGIIITGWQ 
      RYDHFAVLC ELLPVSLPS LAMSLRVIR GYQDSPLSP 
      PTEVAKLLG CEQPYALIG PVFGSPKCS YTGGDILEG 
      ALKLQQLKQ EYETILEDS RVKGWMSDY NKNHQFSNP 
      QHVQSVTAP LNRIKGELM QLDGEMTAA MYEVYDNYT 
      VSEWRETYI KPFEREVRA MVDARDKIL AKNYWPRRP 
      LLQDGDEL
    CDS sequence
    • >TC012468 RA
      atgaaaaaa ctccactgg ttcaacgta tcatccatt 
      ggcaaaccc ctaaaatta ttcgtaacc ttcgcactg 
      atcctgact gttcttttt attacgtat tttacttat 
      cttaaacct aaaccatcc ccaactaaa gtccctttc 
      caacaccga attttcgac gaatataaa cacatgtct 
      caccaagaa acgccgttc aagggggct aaaattgtg 
      cacttagac ttgaaaggg gcccctccg aaaatctcg 
      tactacaaa accctcttt tcgctttta tctaaattt 
      ggtgcgact ggagtgttg attgaatat gaagacatg 
      tttccgtac aattctcct ttgttgaaa aatgtatca 
      gcactgaat gcttacacg gttgaagat attcactac 
      ataaacaag ctagcgttg gaaaacaaa ctggaagtg 
      atcccattg ttgcaaacc ttcggccat ttagagttt 
      ctgttgaaa ctggaggag tttagcgaa ttgcgggaa 
      gtaccggag tatccgcaa gtgatttgt cccacgcat 
      gagaaaacc ttgagtgtt ttgatggaa atgatcgac 
      caagtgttg caaatgcat ccacattcc aagataatt 
      cacattggc gccgatgaa gtttactat ctgggattt 
      tgcgacagg tgtgccaac attatgaac aaattcaac 
      ttgtcaaaa aatatgctg tttttggaa catatcaac 
      gcaatcact actcgaata cgtaaaaaa catccccac 
      ttgcgtatt ctgatgtgg gacgatgaa ttcagatcg 
      ttcagtgtc aaagaactg aaaagtggg tttttggac 
      aagggcgtc caaccagtg gtttggaag tactcaaag 
      gacgtctac gaggaattg gggcccagc ttatgggaa 
      atgtactcg gaggtgttc cctaaagta tggatcgcg 
      agcgctttt aaaggagcc acaggtagc aaccagtac 
      gtctctgac gtcacccac tacgtccag aaccaccgc 
      tcctggcta tccctgatg tcggagtac tcccacagg 
      atcaccttc gagggcatc atcatcacg ggctggcag 
      cgctacgac cacttcgcc gtcctttgc gagcttctc 
      ccagtgagt ctcccctca ctagcaatg agcctccga 
      gtcatccgc ggctaccaa gactcgccc ctgagcccc 
      ccgaccgaa gtggccaaa cttctaggc tgcgagcag 
      ccctacgcc ctcatcggg cccgtgttc ggcagcccc 
      aagtgctcc tacacgggc ggcgacatc ctcgagggg 
      gccctcaag ctccagcag ctcaagcag gagtacgag 
      acgattcta gaggactcg agagtcaag ggctggatg 
      agcgattat aacaaaaac caccagttt tccaacccg 
      caacatgtg cagtccgtt acagcgccc ttgaaccgg 
      atcaagggc gagttgatg caactagac ggggaaatg 
      acggcagct atgtacgaa gtttacgat aattacacg 
      gttagtgag tggagagag acgtacatt aagccgttc 
      gagagggag gtcagggcc atggttgat gcgagagat 
      aaaattttg gcgaaaaat tattggccc aggaggccg 
      ttattgcaa gacggcgac gagttgtga
    mRNA sequence
    • >TC012468 RA
      caatataaa cataaccta aattttcca caacgactc 
      gattttaat ttctaacga aaaaaccaa tccgacatg 
      cgttcattt tcgaagaaa aaatgaaaa aactccact 
      ggttcaacg tatcatcca ttggcaaac ccctaaaat 
      tattcgtaa ccttcgcac tgatcctga ctgttcttt 
      ttattacgt attttactt atcttaaac ctaaaccat 
      ccccaacta aagtccctt tccaacacc gaattttcg 
      acgaatata aacacatgt ctcaccaag aaacgccgt 
      tcaaggggg ctaaaattg tgcacttag acttgaaag 
      gggcccctc cgaaaatct cgtactaca aaaccctct 
      tttcgcttt tatctaaat ttggtgcga ctggagtgt 
      tgattgaat atgaagaca tgtttccgt acaattctc 
      ctttgttga aaaatgtat cagcactga atgcttaca 
      cggttgaag atattcact acataaaca agctagcgt 
      tggaaaaca aactggaag tgatcccat tgttgcaaa 
      ccttcggcc atttagagt ttctgttga aactggagg 
      agtttagcg aattgcggg aagtaccgg agtatccgc 
      aagtgattt gtcccacgc atgagaaaa ccttgagtg 
      ttttgatgg aaatgatcg accaagtgt tgcaaatgc 
      atccacatt ccaagataa ttcacattg gcgccgatg 
      aagtttact atctgggat tttgcgaca ggtgtgcca 
      acattatga acaaattca acttgtcaa aaaatatgc 
      tgtttttgg aacatatca acgcaatca ctactcgaa 
      tacgtaaaa aacatcccc acttgcgta ttctgatgt 
      gggacgatg aattcagat cgttcagtg tcaaagaac 
      tgaaaagtg ggtttttgg acaagggcg tccaaccag 
      tggtttgga agtactcaa aggacgtct acgaggaat 
      tggggccca gcttatggg aaatgtact cggaggtgt 
      tccctaaag tatggatcg cgagcgctt ttaaaggag 
      ccacaggta gcaaccagt acgtctctg acgtcaccc 
      actacgtcc agaaccacc gctcctggc tatccctga 
      tgtcggagt actcccaca ggatcacct tcgagggca 
      tcatcatca cgggctggc agcgctacg accacttcg 
      ccgtccttt gcgagcttc tcccagtga gtctcccct 
      cactagcaa tgagcctcc gagtcatcc gcggctacc 
      aagactcgc ccctgagcc ccccgaccg aagtggcca 
      aacttctag gctgcgagc agccctacg ccctcatcg 
      ggcccgtgt tcggcagcc ccaagtgct cctacacgg 
      gcggcgaca tcctcgagg gggccctca agctccagc 
      agctcaagc aggagtacg agacgattc tagaggact 
      cgagagtca agggctgga tgagcgatt ataacaaaa 
      accaccagt tttccaacc cgcaacatg tgcagtccg 
      ttacagcgc ccttgaacc ggatcaagg gcgagttga 
      tgcaactag acggggaaa tgacggcag ctatgtacg 
      aagtttacg ataattaca cggttagtg agtggagag 
      agacgtaca ttaagccgt tcgagaggg aggtcaggg 
      ccatggttg atgcgagag ataaaattt tggcgaaaa 
      attattggc ccaggaggc cgttattgc aagacggcg 
      acgagttgt gaatgattt ttgtcaatt ttaagcaca 
      aaagcacag agaatctca cgtgaatct tttcaaatt 
      tacttactc aatattgtt acacgtata gcttctaag 
      tgccaaatg tttacatac tgatattat acaaaataa 
      tcttggggt ttttctagt ctagggggt tattgttat 
      ttatgtgac tgttttctg tattgtaat aaagcgttg 
      tgaatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY