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Gene information
General information
TC012019 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7801886..7803553
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012019
    iBeetle screen iB_06457

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012019 RA
      MGEIKTEPL DGAEDQKEE ELTYEQKVN 
NCNTIAKPM ASKKLTKKV HKLVKKAVK 
HKTYVRNGL KDVQKRIRK GETGIVIFA 
GDITPIDIM CHLPAVCED RDIPYVYVP 
SRRDLGGAM GIMRGCMLA LVRPHDDYK 
DMFNELKDE IKTLAVEL
    CDS sequence
    • >TC012019 RA
      atgggtgag ataaaaacg gagcctctg gacggggct 
gaggaccag aaagaggaa gaattgacc tacgagcaa 
aaagtcaac aactgtaat acaatagca aagcctatg 
gcgtcgaaa aaattgaca aaaaaagta cacaaattg 
gtgaaaaaa gccgtaaaa cacaaaacg tacgtccgg 
aacgggctg aaggatgtg caaaaacgg attcgcaag 
ggggagaca gggattgtg atcttcgct ggagacatt 
actcccatc gatataatg tgccactta ccggcggtt 
tgtgaggac agagacatt ccgtacgtg tatgtgcct 
agtaggcgg gacttgggg ggcgcaatg gggatcatg 
aggggctgc atgttggcg ttagtgcgc ccccatgac 
gactacaag gatatgttt aacgagtta aaggatgaa 
atcaaaact ttggctgta gaattgtaa
    mRNA sequence
    • >TC012019 RA
      caaaaaatt caaattaaa attttgccg cttgaaact 
actaaagcg ccacctaca gttcgatca tttgttgtg 
ttcccaaca attcattaa ttaaaaaat taagacaag 
tttacgact aattagtgt gtgaaacag agcaagtaa 
gtcttgttg ttgacacta cctaacctc aaatttttg 
atttgcgtt gcacgtgtt aaaccatgg gtgagataa 
aaacggagc ctctggacg gggctgagg accagaaag 
aggaagaat tgacctacg agcaaaaag tcaacaact 
gtaatacaa tagcaaagc ctatggcgt cgaaaaaat 
tgacaaaaa aagtacaca aattggtga aaaaagccg 
taaaacaca aaacgtacg tccggaacg ggctgaagg 
atgtgcaaa aacggattc gcaaggggg agacaggga 
ttgtgatct tcgctggag acattactc ccatcgata 
taatgtgcc acttaccgg cggtttgtg aggacagag 
acattccgt acgtgtatg tgcctagta ggcgggact 
tggggggcg caatgggga tcatgaggg gctgcatgt 
tggcgttag tgcgccccc atgacgact acaaggata 
tgtttaacg agttaaagg atgaaatca aaactttgg 
ctgtagaat tgtaagtta ataaaaagc tggcgttta 
atacaaata tttattaga atagcaaat tattgcaca 
catacatga ttgttgagt aggtagatg tttatataa 
ggaaaatca tacacatct ccctatagt ttgccttaa 
aatgagaaa caaaccata aataaaatt tatattcca 
ctggcatta ataggtgag ggtgcatat ttcagaatt 
ggtgcttag actggaaaa gcgtcaatt tgtggaata 
taaacttta cgttaaaat cagttggtg tcacctagt 
tcgagccga aagcaaact ttaatgtag gctatactg 
acttccaat ttccgagat ggtgctctt tatacagtg 
aactaaaaa ctacaccta acgtaacac agtatactt 
tgtatagtc cacactaac ttaaaaaat aataaacga 
acacataag atgacaagt ttgcgtaat aaaaaacgg 
ggagcgcct aacttgtcg tcaaaaagt tggtcctaa 
aacgaataa ataaaaaga cagtgagac actgatctt 
taatattcg cacatgagt agaaaaatc cttaatatg 
ggagtcggg aaaactaaa ccgaccgtc ctagcacca 
cctaaaact aaaattgac gcggcgtct acttgatgt 
agaaaaata ggtgtttcg tcgatattc tctcgctaa 
gccacctag tgcactcct ggggacact cgaaagcag 
cagtcaggt cggtctaac cttccagga tacgaagtc 
taaaacgtg caagtgtac agagtacag gtaaccgat 
ctgaagatg ttgtctcca tgaaacaaa cccctgtct 
tgctgtcgt ccctctatc cacaaccat tgccaccaa 
taaatcatt attacatcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY